Maize-associated pteridovirus
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q9D1K2|A0A3Q9D1K2_9VIRU RNA helicase OS=Maize-associated pteridovirus OX=2497338 PE=4 SV=1
MM1 pKa = 7.24 FPEE4 pKa = 5.81 SIAQMLKK11 pKa = 9.63 SRR13 pKa = 11.84 VRR15 pKa = 11.84 RR16 pKa = 11.84 SASAAVFAAAAASADD31 pKa = 3.53 AVAGTLRR38 pKa = 11.84 EE39 pKa = 4.13 FEE41 pKa = 3.99 ISLARR46 pKa = 11.84 DD47 pKa = 3.47 LDD49 pKa = 3.82 AVEE52 pKa = 4.54 YY53 pKa = 10.45 EE54 pKa = 4.39 SFVTDD59 pKa = 3.7 SEE61 pKa = 4.77 GVPAMVDD68 pKa = 3.2 DD69 pKa = 5.61 APITRR74 pKa = 11.84 CGPARR79 pKa = 11.84 YY80 pKa = 8.74 VWGVAALSGYY90 pKa = 8.03 TPTVIGRR97 pKa = 11.84 LFRR100 pKa = 11.84 HH101 pKa = 5.91 KK102 pKa = 10.56 RR103 pKa = 11.84 PMGISGSVEE112 pKa = 3.72 PLSIDD117 pKa = 4.15 DD118 pKa = 3.97 YY119 pKa = 11.57 TDD121 pKa = 2.88 VNYY124 pKa = 10.61 CVEE127 pKa = 3.85 HH128 pKa = 7.37 DD129 pKa = 3.7 IFYY132 pKa = 10.43 EE133 pKa = 4.3 CTQEE137 pKa = 4.32 SVCMRR142 pKa = 11.84 CVDD145 pKa = 3.84 AGVMFTPNVLDD156 pKa = 4.2 RR157 pKa = 11.84 DD158 pKa = 4.05 LVPYY162 pKa = 9.53 TRR164 pKa = 11.84 IRR166 pKa = 11.84 GSEE169 pKa = 4.46 LIPTYY174 pKa = 10.69 VGRR177 pKa = 11.84 EE178 pKa = 3.96 YY179 pKa = 11.34 LNGIPRR185 pKa = 11.84 HH186 pKa = 5.15 HH187 pKa = 7.63 WYY189 pKa = 10.6 LSSQSVLTCACGSSDD204 pKa = 4.98 HH205 pKa = 6.55 EE206 pKa = 4.6 FEE208 pKa = 4.91 FDD210 pKa = 3.48 GFPPIWFKK218 pKa = 11.15 PPASFAYY225 pKa = 10.36 ALFPTSLRR233 pKa = 11.84 EE234 pKa = 3.91 EE235 pKa = 5.03 GEE237 pKa = 4.18 TSSLVLSSFGYY248 pKa = 9.92 IVGRR252 pKa = 11.84 YY253 pKa = 8.11 GKK255 pKa = 9.27 RR256 pKa = 11.84 QQSQVPAGQVARR268 pKa = 11.84 SSVSLGLL275 pKa = 3.7
Molecular weight: 30.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.965
IPC2_protein 5.13
IPC_protein 5.054
Toseland 5.003
ProMoST 5.207
Dawson 5.054
Bjellqvist 5.181
Wikipedia 4.94
Rodwell 4.978
Grimsley 4.94
Solomon 5.054
Lehninger 5.016
Nozaki 5.181
DTASelect 5.334
Thurlkill 5.016
EMBOSS 4.978
Sillero 5.258
Patrickios 3.452
IPC_peptide 5.067
IPC2_peptide 5.258
IPC2.peptide.svr19 5.197
Protein with the highest isoelectric point:
>tr|A0A3Q9D1K1|A0A3Q9D1K1_9VIRU ORF2 OS=Maize-associated pteridovirus OX=2497338 PE=4 SV=1
MM1 pKa = 7.86 ASGSKK6 pKa = 10.34 AKK8 pKa = 10.8 SPLDD12 pKa = 3.5 KK13 pKa = 11.26 LLDD16 pKa = 3.47 QALALGFDD24 pKa = 4.49 VKK26 pKa = 10.93 RR27 pKa = 11.84 KK28 pKa = 10.12 GNDD31 pKa = 3.29 GASFSQRR38 pKa = 11.84 DD39 pKa = 3.46 GASKK43 pKa = 10.96 VKK45 pKa = 10.12 ILKK48 pKa = 8.86 TFLANQRR55 pKa = 11.84 KK56 pKa = 9.03 KK57 pKa = 10.86 NKK59 pKa = 9.43 GVAADD64 pKa = 3.72 VPGQSHH70 pKa = 5.58 VANPARR76 pKa = 11.84 VVQEE80 pKa = 3.41 QSARR84 pKa = 11.84 LEE86 pKa = 4.29 WIPLISKK93 pKa = 7.62 WTALDD98 pKa = 3.25 HH99 pKa = 5.6 TKK101 pKa = 10.37 EE102 pKa = 4.09 YY103 pKa = 10.71 QRR105 pKa = 11.84 LPMTSEE111 pKa = 3.53 IQKK114 pKa = 10.27 EE115 pKa = 4.35 KK116 pKa = 9.12 VHH118 pKa = 6.69 KK119 pKa = 10.56 LVFSLKK125 pKa = 9.82 IAGSVALTEE134 pKa = 4.26 PVAKK138 pKa = 9.95 VVPVFNTNIKK148 pKa = 10.82 GDD150 pKa = 4.05 DD151 pKa = 3.68 LKK153 pKa = 10.33 TASVEE158 pKa = 4.21 SDD160 pKa = 3.65 FSSLIISNQNGEE172 pKa = 4.21 SPYY175 pKa = 10.3 VLEE178 pKa = 4.38 FAEE181 pKa = 4.69 PCTTEE186 pKa = 5.0 LIGAKK191 pKa = 10.01 LSMVVATGNTEE202 pKa = 4.22 VGTEE206 pKa = 3.68 WLYY209 pKa = 11.06 YY210 pKa = 8.38 KK211 pKa = 10.61 AWMSAEE217 pKa = 4.43 VASHH221 pKa = 7.03 ASPNLEE227 pKa = 4.0 NAFVAALLDD236 pKa = 3.96 SCLSRR241 pKa = 11.84 AFNN244 pKa = 3.89
Molecular weight: 26.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.838
IPC2_protein 7.922
IPC_protein 7.936
Toseland 8.58
ProMoST 8.39
Dawson 8.829
Bjellqvist 8.726
Wikipedia 8.858
Rodwell 9.019
Grimsley 8.448
Solomon 9.048
Lehninger 9.048
Nozaki 8.975
DTASelect 8.639
Thurlkill 8.799
EMBOSS 9.048
Sillero 8.99
Patrickios 5.105
IPC_peptide 9.048
IPC2_peptide 7.38
IPC2.peptide.svr19 7.538
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2706
244
1862
676.5
75.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.948 ± 1.102
2.106 ± 0.368
6.245 ± 0.498
5.617 ± 0.179
4.582 ± 0.304
5.728 ± 1.155
2.402 ± 0.393
5.026 ± 0.279
5.876 ± 0.932
8.684 ± 0.519
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.03 ± 0.34
3.732 ± 0.521
4.324 ± 0.472
2.993 ± 0.284
5.58 ± 0.651
8.537 ± 0.697
5.765 ± 0.318
8.389 ± 0.267
1.072 ± 0.097
3.363 ± 0.435
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here