Amino acid dipepetide frequency for Geobacillus kaustophilus (strain HTA426)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.1AlaAla: 11.1 ± 0.173	0.824AlaCys: 0.824 ± 0.033	4.487AlaAsp: 4.487 ± 0.072	6.389AlaGlu: 6.389 ± 0.094	3.736AlaPhe: 3.736 ± 0.065	6.567AlaGly: 6.567 ± 0.093	1.776AlaHis: 1.776 ± 0.045	6.052AlaIle: 6.052 ± 0.083	5.696AlaLys: 5.696 ± 0.081	9.77AlaLeu: 9.77 ± 0.113	2.618AlaMet: 2.618 ± 0.059	2.763AlaAsn: 2.763 ± 0.057	3.307AlaPro: 3.307 ± 0.077	2.565AlaGln: 2.565 ± 0.056	4.868AlaArg: 4.868 ± 0.077	4.373AlaSer: 4.373 ± 0.073	3.621AlaThr: 3.621 ± 0.072	7.992AlaVal: 7.992 ± 0.108	1.04AlaTrp: 1.04 ± 0.034	2.712AlaTyr: 2.712 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.643CysAla: 0.643 ± 0.028	0.153CysCys: 0.153 ± 0.012	0.432CysAsp: 0.432 ± 0.021	0.547CysGlu: 0.547 ± 0.024	0.408CysPhe: 0.408 ± 0.021	0.806CysGly: 0.806 ± 0.035	0.243CysHis: 0.243 ± 0.016	0.459CysIle: 0.459 ± 0.021	0.282CysLys: 0.282 ± 0.016	0.908CysLeu: 0.908 ± 0.027	0.171CysMet: 0.171 ± 0.012	0.246CysAsn: 0.246 ± 0.016	0.513CysPro: 0.513 ± 0.027	0.347CysGln: 0.347 ± 0.022	0.629CysArg: 0.629 ± 0.026	0.48CysSer: 0.48 ± 0.024	0.45CysThr: 0.45 ± 0.02	0.522CysVal: 0.522 ± 0.023	0.107CysTrp: 0.107 ± 0.01	0.276CysTyr: 0.276 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.779AspAla: 3.779 ± 0.065	0.417AspCys: 0.417 ± 0.023	2.712AspAsp: 2.712 ± 0.063	4.766AspGlu: 4.766 ± 0.083	1.884AspPhe: 1.884 ± 0.046	4.213AspGly: 4.213 ± 0.079	1.113AspHis: 1.113 ± 0.035	3.516AspIle: 3.516 ± 0.065	2.03AspLys: 2.03 ± 0.051	4.39AspLeu: 4.39 ± 0.073	1.135AspMet: 1.135 ± 0.04	1.152AspAsn: 1.152 ± 0.038	2.349AspPro: 2.349 ± 0.048	1.435AspGln: 1.435 ± 0.039	3.02AspArg: 3.02 ± 0.06	1.79AspSer: 1.79 ± 0.044	1.735AspThr: 1.735 ± 0.044	4.52AspVal: 4.52 ± 0.063	0.742AspTrp: 0.742 ± 0.031	1.922AspTyr: 1.922 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	7.621GluAla: 7.621 ± 0.097	0.413GluCys: 0.413 ± 0.02	2.799GluAsp: 2.799 ± 0.057	6.736GluGlu: 6.736 ± 0.116	2.14GluPhe: 2.14 ± 0.047	4.258GluGly: 4.258 ± 0.075	1.699GluHis: 1.699 ± 0.045	4.21GluIle: 4.21 ± 0.075	5.273GluLys: 5.273 ± 0.085	7.282GluLeu: 7.282 ± 0.097	2.237GluMet: 2.237 ± 0.053	2.318GluAsn: 2.318 ± 0.053	2.405GluPro: 2.405 ± 0.046	4.279GluGln: 4.279 ± 0.077	5.989GluArg: 5.989 ± 0.096	2.511GluSer: 2.511 ± 0.05	4.252GluThr: 4.252 ± 0.072	4.698GluVal: 4.698 ± 0.08	1.169GluTrp: 1.169 ± 0.032	1.895GluTyr: 1.895 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	4.065PheAla: 4.065 ± 0.067	0.446PheCys: 0.446 ± 0.021	2.111PheAsp: 2.111 ± 0.047	2.136PheGlu: 2.136 ± 0.05	2.243PhePhe: 2.243 ± 0.059	3.308PheGly: 3.308 ± 0.057	1.107PheHis: 1.107 ± 0.034	2.609PheIle: 2.609 ± 0.065	1.307PheLys: 1.307 ± 0.037	4.49PheLeu: 4.49 ± 0.089	0.888PheMet: 0.888 ± 0.028	0.999PheAsn: 0.999 ± 0.028	1.818PhePro: 1.818 ± 0.042	1.398PheGln: 1.398 ± 0.039	2.366PheArg: 2.366 ± 0.052	2.755PheSer: 2.755 ± 0.062	2.108PheThr: 2.108 ± 0.048	3.438PheVal: 3.438 ± 0.061	0.569PheTrp: 0.569 ± 0.025	1.515PheTyr: 1.515 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	6.357GlyAla: 6.357 ± 0.089	0.738GlyCys: 0.738 ± 0.024	3.366GlyAsp: 3.366 ± 0.066	4.916GlyGlu: 4.916 ± 0.071	2.961GlyPhe: 2.961 ± 0.053	5.429GlyGly: 5.429 ± 0.091	1.621GlyHis: 1.621 ± 0.044	4.864GlyIle: 4.864 ± 0.072	4.827GlyLys: 4.827 ± 0.083	6.849GlyLeu: 6.849 ± 0.094	2.336GlyMet: 2.336 ± 0.057	2.148GlyAsn: 2.148 ± 0.062	2.135GlyPro: 2.135 ± 0.055	2.451GlyGln: 2.451 ± 0.057	4.434GlyArg: 4.434 ± 0.073	3.362GlySer: 3.362 ± 0.06	3.984GlyThr: 3.984 ± 0.07	5.878GlyVal: 5.878 ± 0.09	1.033GlyTrp: 1.033 ± 0.035	2.772GlyTyr: 2.772 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.656HisAla: 1.656 ± 0.042	0.249HisCys: 0.249 ± 0.015	1.088HisAsp: 1.088 ± 0.033	1.644HisGlu: 1.644 ± 0.043	1.009HisPhe: 1.009 ± 0.032	1.859HisGly: 1.859 ± 0.048	0.834HisHis: 0.834 ± 0.039	1.538HisIle: 1.538 ± 0.04	0.724HisLys: 0.724 ± 0.026	2.344HisLeu: 2.344 ± 0.055	0.56HisMet: 0.56 ± 0.027	0.64HisAsn: 0.64 ± 0.025	1.566HisPro: 1.566 ± 0.038	0.855HisGln: 0.855 ± 0.028	1.505HisArg: 1.505 ± 0.041	1.055HisSer: 1.055 ± 0.029	0.955HisThr: 0.955 ± 0.032	1.729HisVal: 1.729 ± 0.041	0.329HisTrp: 0.329 ± 0.021	0.943HisTyr: 0.943 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.553IleAla: 6.553 ± 0.096	0.581IleCys: 0.581 ± 0.023	4.03IleAsp: 4.03 ± 0.076	5.086IleGlu: 5.086 ± 0.077	2.226IlePhe: 2.226 ± 0.059	5.478IleGly: 5.478 ± 0.083	1.614IleHis: 1.614 ± 0.042	4.036IleIle: 4.036 ± 0.073	2.683IleLys: 2.683 ± 0.065	4.658IleLeu: 4.658 ± 0.086	1.239IleMet: 1.239 ± 0.04	1.93IleAsn: 1.93 ± 0.049	3.052IlePro: 3.052 ± 0.054	2.182IleGln: 2.182 ± 0.049	4.032IleArg: 4.032 ± 0.071	3.333IleSer: 3.333 ± 0.058	2.995IleThr: 2.995 ± 0.058	5.539IleVal: 5.539 ± 0.078	0.578IleTrp: 0.578 ± 0.023	1.962IleTyr: 1.962 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.868LysAla: 4.868 ± 0.082	0.281LysCys: 0.281 ± 0.018	2.378LysAsp: 2.378 ± 0.052	5.531LysGlu: 5.531 ± 0.085	1.265LysPhe: 1.265 ± 0.03	3.919LysGly: 3.919 ± 0.068	1.095LysHis: 1.095 ± 0.032	3.025LysIle: 3.025 ± 0.066	4.164LysLys: 4.164 ± 0.078	4.673LysLeu: 4.673 ± 0.068	1.617LysMet: 1.617 ± 0.039	1.843LysAsn: 1.843 ± 0.049	2.372LysPro: 2.372 ± 0.047	3.372LysGln: 3.372 ± 0.069	4.206LysArg: 4.206 ± 0.067	2.117LysSer: 2.117 ± 0.051	3.492LysThr: 3.492 ± 0.058	3.667LysVal: 3.667 ± 0.069	0.8LysTrp: 0.8 ± 0.025	1.62LysTyr: 1.62 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	10.274LeuAla: 10.274 ± 0.118	0.865LeuCys: 0.865 ± 0.027	4.887LeuAsp: 4.887 ± 0.07	6.729LeuGlu: 6.729 ± 0.084	5.005LeuPhe: 5.005 ± 0.085	6.798LeuGly: 6.798 ± 0.09	2.261LeuHis: 2.261 ± 0.052	5.714LeuIle: 5.714 ± 0.075	5.208LeuLys: 5.208 ± 0.08	10.755LeuLeu: 10.755 ± 0.147	2.205LeuMet: 2.205 ± 0.051	2.872LeuAsn: 2.872 ± 0.054	4.653LeuPro: 4.653 ± 0.066	3.28LeuGln: 3.28 ± 0.062	5.545LeuArg: 5.545 ± 0.08	6.147LeuSer: 6.147 ± 0.093	4.895LeuThr: 4.895 ± 0.067	6.992LeuVal: 6.992 ± 0.099	1.077LeuTrp: 1.077 ± 0.037	3.228LeuTyr: 3.228 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.868MetAla: 2.868 ± 0.06	0.156MetCys: 0.156 ± 0.013	1.275MetAsp: 1.275 ± 0.038	2.041MetGlu: 2.041 ± 0.052	0.961MetPhe: 0.961 ± 0.03	1.626MetGly: 1.626 ± 0.045	0.449MetHis: 0.449 ± 0.022	1.774MetIle: 1.774 ± 0.045	2.135MetLys: 2.135 ± 0.049	2.251MetLeu: 2.251 ± 0.056	0.948MetMet: 0.948 ± 0.035	1.235MetAsn: 1.235 ± 0.036	1.157MetPro: 1.157 ± 0.037	0.823MetGln: 0.823 ± 0.029	1.413MetArg: 1.413 ± 0.042	1.319MetSer: 1.319 ± 0.037	1.622MetThr: 1.622 ± 0.038	1.654MetVal: 1.654 ± 0.04	0.219MetTrp: 0.219 ± 0.015	0.711MetTyr: 0.711 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.541AsnAla: 2.541 ± 0.052	0.225AsnCys: 0.225 ± 0.017	1.672AsnAsp: 1.672 ± 0.037	2.803AsnGlu: 2.803 ± 0.059	1.006AsnPhe: 1.006 ± 0.035	3.076AsnGly: 3.076 ± 0.057	0.686AsnHis: 0.686 ± 0.027	2.202AsnIle: 2.202 ± 0.048	1.593AsnLys: 1.593 ± 0.045	2.233AsnLeu: 2.233 ± 0.051	0.87AsnMet: 0.87 ± 0.038	1.024AsnAsn: 1.024 ± 0.039	1.753AsnPro: 1.753 ± 0.042	1.083AsnGln: 1.083 ± 0.039	1.817AsnArg: 1.817 ± 0.043	1.231AsnSer: 1.231 ± 0.036	1.318AsnThr: 1.318 ± 0.038	2.615AsnVal: 2.615 ± 0.053	0.357AsnTrp: 0.357 ± 0.02	0.912AsnTyr: 0.912 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	3.247ProAla: 3.247 ± 0.059	0.31ProCys: 0.31 ± 0.018	2.483ProAsp: 2.483 ± 0.05	3.152ProGlu: 3.152 ± 0.061	2.334ProPhe: 2.334 ± 0.047	2.783ProGly: 2.783 ± 0.06	1.175ProHis: 1.175 ± 0.034	2.625ProIle: 2.625 ± 0.045	2.34ProLys: 2.34 ± 0.05	4.797ProLeu: 4.797 ± 0.068	1.01ProMet: 1.01 ± 0.033	1.675ProAsn: 1.675 ± 0.045	1.779ProPro: 1.779 ± 0.052	1.187ProGln: 1.187 ± 0.036	1.816ProArg: 1.816 ± 0.047	2.664ProSer: 2.664 ± 0.053	2.105ProThr: 2.105 ± 0.055	3.265ProVal: 3.265 ± 0.058	0.466ProTrp: 0.466 ± 0.021	1.62ProTyr: 1.62 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.573GlnAla: 3.573 ± 0.067	0.309GlnCys: 0.309 ± 0.019	1.207GlnAsp: 1.207 ± 0.037	2.571GlnGlu: 2.571 ± 0.062	1.543GlnPhe: 1.543 ± 0.042	2.297GlnGly: 2.297 ± 0.045	0.833GlnHis: 0.833 ± 0.031	2.179GlnIle: 2.179 ± 0.045	2.345GlnLys: 2.345 ± 0.06	4.265GlnLeu: 4.265 ± 0.079	1.13GlnMet: 1.13 ± 0.031	1.094GlnAsn: 1.094 ± 0.034	1.412GlnPro: 1.412 ± 0.042	1.711GlnGln: 1.711 ± 0.055	2.168GlnArg: 2.168 ± 0.051	1.676GlnSer: 1.676 ± 0.046	2.147GlnThr: 2.147 ± 0.049	2.148GlnVal: 2.148 ± 0.055	0.616GlnTrp: 0.616 ± 0.023	1.297GlnTyr: 1.297 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	4.503ArgAla: 4.503 ± 0.069	0.565ArgCys: 0.565 ± 0.023	2.723ArgAsp: 2.723 ± 0.058	4.476ArgGlu: 4.476 ± 0.076	2.703ArgPhe: 2.703 ± 0.05	3.588ArgGly: 3.588 ± 0.058	1.659ArgHis: 1.659 ± 0.044	3.58ArgIle: 3.58 ± 0.058	3.492ArgLys: 3.492 ± 0.068	7.118ArgLeu: 7.118 ± 0.104	1.786ArgMet: 1.786 ± 0.042	1.706ArgAsn: 1.706 ± 0.039	2.526ArgPro: 2.526 ± 0.055	2.888ArgGln: 2.888 ± 0.058	4.597ArgArg: 4.597 ± 0.094	2.802ArgSer: 2.802 ± 0.052	2.888ArgThr: 2.888 ± 0.054	3.796ArgVal: 3.796 ± 0.066	0.943ArgTrp: 0.943 ± 0.036	2.415ArgTyr: 2.415 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	4.138SerAla: 4.138 ± 0.074	0.446SerCys: 0.446 ± 0.02	2.272SerAsp: 2.272 ± 0.046	3.026SerGlu: 3.026 ± 0.052	2.747SerPhe: 2.747 ± 0.054	4.112SerGly: 4.112 ± 0.068	1.114SerHis: 1.114 ± 0.033	3.217SerIle: 3.217 ± 0.061	2.256SerLys: 2.256 ± 0.055	5.565SerLeu: 5.565 ± 0.08	1.272SerMet: 1.272 ± 0.031	1.336SerAsn: 1.336 ± 0.036	2.337SerPro: 2.337 ± 0.054	1.415SerGln: 1.415 ± 0.037	2.695SerArg: 2.695 ± 0.045	2.942SerSer: 2.942 ± 0.068	2.251SerThr: 2.251 ± 0.053	3.769SerVal: 3.769 ± 0.068	0.636SerTrp: 0.636 ± 0.028	1.687SerTyr: 1.687 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	4.489ThrAla: 4.489 ± 0.077	0.392ThrCys: 0.392 ± 0.023	2.4ThrAsp: 2.4 ± 0.051	2.981ThrGlu: 2.981 ± 0.053	2.304ThrPhe: 2.304 ± 0.047	3.91ThrGly: 3.91 ± 0.075	0.983ThrHis: 0.983 ± 0.033	3.798ThrIle: 3.798 ± 0.057	3.042ThrLys: 3.042 ± 0.058	4.752ThrLeu: 4.752 ± 0.074	1.36ThrMet: 1.36 ± 0.037	1.868ThrAsn: 1.868 ± 0.04	2.335ThrPro: 2.335 ± 0.055	1.057ThrGln: 1.057 ± 0.033	2.052ThrArg: 2.052 ± 0.05	2.427ThrSer: 2.427 ± 0.058	2.553ThrThr: 2.553 ± 0.059	4.691ThrVal: 4.691 ± 0.071	0.589ThrTrp: 0.589 ± 0.026	1.802ThrTyr: 1.802 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	6.498ValAla: 6.498 ± 0.093	0.795ValCys: 0.795 ± 0.032	3.826ValAsp: 3.826 ± 0.057	5.022ValGlu: 5.022 ± 0.075	3.176ValPhe: 3.176 ± 0.059	5.018ValGly: 5.018 ± 0.075	1.758ValHis: 1.758 ± 0.046	5.397ValIle: 5.397 ± 0.082	4.522ValLys: 4.522 ± 0.067	7.524ValLeu: 7.524 ± 0.093	1.979ValMet: 1.979 ± 0.039	2.572ValAsn: 2.572 ± 0.058	3.491ValPro: 3.491 ± 0.073	2.503ValGln: 2.503 ± 0.056	4.385ValArg: 4.385 ± 0.07	4.138ValSer: 4.138 ± 0.062	4.022ValThr: 4.022 ± 0.069	5.985ValVal: 5.985 ± 0.087	0.975ValTrp: 0.975 ± 0.035	2.682ValTyr: 2.682 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.909TrpAla: 0.909 ± 0.038	0.105TrpCys: 0.105 ± 0.012	0.518TrpAsp: 0.518 ± 0.025	0.859TrpGlu: 0.859 ± 0.031	0.553TrpPhe: 0.553 ± 0.024	0.722TrpGly: 0.722 ± 0.027	0.289TrpHis: 0.289 ± 0.016	0.819TrpIle: 0.819 ± 0.028	0.906TrpLys: 0.906 ± 0.031	1.779TrpLeu: 1.779 ± 0.051	0.44TrpMet: 0.44 ± 0.02	0.526TrpAsn: 0.526 ± 0.02	0.472TrpPro: 0.472 ± 0.024	0.577TrpGln: 0.577 ± 0.026	0.839TrpArg: 0.839 ± 0.033	0.566TrpSer: 0.566 ± 0.026	0.671TrpThr: 0.671 ± 0.027	0.806TrpVal: 0.806 ± 0.03	0.201TrpTrp: 0.201 ± 0.014	0.364TrpTyr: 0.364 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.575TyrAla: 2.575 ± 0.054	0.354TyrCys: 0.354 ± 0.019	1.957TyrAsp: 1.957 ± 0.044	2.776TyrGlu: 2.776 ± 0.057	1.471TyrPhe: 1.471 ± 0.045	2.695TyrGly: 2.695 ± 0.049	0.772TyrHis: 0.772 ± 0.028	2.171TyrIle: 2.171 ± 0.047	1.48TyrLys: 1.48 ± 0.038	2.988TyrLeu: 2.988 ± 0.066	0.837TyrMet: 0.837 ± 0.029	1.068TyrAsn: 1.068 ± 0.033	1.402TyrPro: 1.402 ± 0.041	1.116TyrGln: 1.116 ± 0.038	2.373TyrArg: 2.373 ± 0.051	1.545TyrSer: 1.545 ± 0.042	1.72TyrThr: 1.72 ± 0.049	2.562TyrVal: 2.562 ± 0.05	0.474TyrTrp: 0.474 ± 0.023	1.307TyrTyr: 1.307 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3516 proteins (1005871 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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