Candidatus [Bacteroides] periocalifornicus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidetes incertae sedis

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1579 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4B934|A0A0Q4B934_9BACT Uncharacterized protein OS=Candidatus [Bacteroides] periocalifornicus OX=1702214 GN=AL399_02645 PE=4 SV=1
MM1 pKa = 6.95NTLKK5 pKa = 10.75GLYY8 pKa = 9.16PDD10 pKa = 4.4YY11 pKa = 10.64EE12 pKa = 4.46PNIAFYY18 pKa = 10.3NAEE21 pKa = 4.14VEE23 pKa = 4.67DD24 pKa = 4.59EE25 pKa = 4.38PACVMVDD32 pKa = 3.9LNLEE36 pKa = 3.87EE37 pKa = 4.23YY38 pKa = 10.91APVEE42 pKa = 4.22GYY44 pKa = 10.75EE45 pKa = 4.19YY46 pKa = 10.19ICVVKK51 pKa = 10.61VLAPEE56 pKa = 4.45GASINGDD63 pKa = 3.37KK64 pKa = 10.49IGRR67 pKa = 11.84LDD69 pKa = 3.71EE70 pKa = 4.63KK71 pKa = 10.44LTEE74 pKa = 4.52HH75 pKa = 6.94LAEE78 pKa = 4.88AYY80 pKa = 9.5GAVWAGSLNWDD91 pKa = 3.78DD92 pKa = 4.23EE93 pKa = 4.55VHH95 pKa = 5.71IFYY98 pKa = 10.95YY99 pKa = 9.24LTEE102 pKa = 4.94EE103 pKa = 3.85IDD105 pKa = 3.34QRR107 pKa = 11.84EE108 pKa = 4.24ILQFPPVKK116 pKa = 9.96AAKK119 pKa = 9.24YY120 pKa = 9.36DD121 pKa = 4.24CIVEE125 pKa = 4.24IAPEE129 pKa = 4.36PEE131 pKa = 3.44WDD133 pKa = 3.42TYY135 pKa = 11.51LALLYY140 pKa = 10.02PDD142 pKa = 4.61DD143 pKa = 4.12YY144 pKa = 11.88GRR146 pKa = 11.84LEE148 pKa = 3.81IEE150 pKa = 3.82NRR152 pKa = 11.84EE153 pKa = 4.17LLGEE157 pKa = 4.16AQGEE161 pKa = 4.18GDD163 pKa = 3.75DD164 pKa = 3.95FSRR167 pKa = 11.84ARR169 pKa = 11.84LVTHH173 pKa = 6.4SSYY176 pKa = 11.13FDD178 pKa = 3.3RR179 pKa = 11.84QEE181 pKa = 3.93DD182 pKa = 3.61LKK184 pKa = 11.41AFCTEE189 pKa = 3.81VEE191 pKa = 4.03RR192 pKa = 11.84LGFRR196 pKa = 11.84IVEE199 pKa = 4.28LEE201 pKa = 4.02DD202 pKa = 3.89TPDD205 pKa = 3.04VDD207 pKa = 5.02ADD209 pKa = 3.77FPYY212 pKa = 9.56GVEE215 pKa = 3.96YY216 pKa = 11.06AKK218 pKa = 10.77VHH220 pKa = 5.7TLLDD224 pKa = 3.5IPKK227 pKa = 8.36ITQEE231 pKa = 4.08LLEE234 pKa = 4.69LTLAHH239 pKa = 6.39NGYY242 pKa = 10.48YY243 pKa = 10.34DD244 pKa = 3.23GWLNTSDD251 pKa = 5.64ADD253 pKa = 4.78IDD255 pKa = 4.02DD256 pKa = 4.07LL257 pKa = 5.61

Molecular weight: 29.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4B339|A0A0Q4B339_9BACT Nitrogen fixation protein NifU OS=Candidatus [Bacteroides] periocalifornicus OX=1702214 GN=AL399_07790 PE=4 SV=1
MM1 pKa = 7.85AEE3 pKa = 3.62QIARR7 pKa = 11.84IRR9 pKa = 11.84LIHH12 pKa = 4.95TAKK15 pKa = 10.42VGEE18 pKa = 4.21KK19 pKa = 10.57FEE21 pKa = 4.27IASVRR26 pKa = 11.84GLGSLTRR33 pKa = 11.84RR34 pKa = 11.84GIARR38 pKa = 11.84SSKK41 pKa = 10.26GGHH44 pKa = 5.38TLRR47 pKa = 11.84SASPLCDD54 pKa = 3.35NMDD57 pKa = 5.33GIHH60 pKa = 6.23LWWRR64 pKa = 11.84EE65 pKa = 3.75NLRR68 pKa = 11.84VAKK71 pKa = 7.9MVCVYY76 pKa = 10.75GEE78 pKa = 3.73PDD80 pKa = 2.85GRR82 pKa = 11.84GNLRR86 pKa = 11.84VAKK89 pKa = 9.2TPPLQSALAGMLLLVALGVVDD110 pKa = 5.68KK111 pKa = 11.37INVFCGGLPMQGRR124 pKa = 11.84GAPRR128 pKa = 11.84RR129 pKa = 11.84YY130 pKa = 10.35KK131 pKa = 10.89NGGDD135 pKa = 3.07HH136 pKa = 6.95ALNRR140 pKa = 11.84EE141 pKa = 3.9GGLIHH146 pKa = 7.5RR147 pKa = 11.84DD148 pKa = 3.29AGNSS152 pKa = 3.32

Molecular weight: 16.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1579

0

1579

605109

56

3444

383.2

42.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.151 ± 0.05

1.063 ± 0.02

4.882 ± 0.04

6.266 ± 0.053

3.782 ± 0.037

8.148 ± 0.055

2.023 ± 0.027

5.097 ± 0.052

4.324 ± 0.055

10.409 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.033

3.553 ± 0.04

4.638 ± 0.038

4.049 ± 0.034

6.268 ± 0.066

5.846 ± 0.05

5.532 ± 0.07

7.648 ± 0.055

1.256 ± 0.022

3.776 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski