Avon-Heathcote Estuary associated circular virus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IM87|A0A0C5IM87_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 4 OX=1618255 PE=4 SV=1
MM1 pKa = 6.07THH3 pKa = 6.82IYY5 pKa = 10.77KK6 pKa = 9.54MADD9 pKa = 2.93VRR11 pKa = 11.84KK12 pKa = 9.24RR13 pKa = 11.84AYY15 pKa = 10.99CMTIHH20 pKa = 6.1NWSEE24 pKa = 3.86EE25 pKa = 4.12TVNQLKK31 pKa = 9.87NLKK34 pKa = 8.99YY35 pKa = 10.0QYY37 pKa = 10.75LIIGNEE43 pKa = 4.1TCPTTDD49 pKa = 2.91RR50 pKa = 11.84KK51 pKa = 10.23HH52 pKa = 5.79LQVYY56 pKa = 10.21AYY58 pKa = 9.05FKK60 pKa = 10.6NALSFKK66 pKa = 10.05SIKK69 pKa = 10.15KK70 pKa = 8.94QFPTAHH76 pKa = 6.55IEE78 pKa = 4.1ASKK81 pKa = 9.4GTPNHH86 pKa = 6.14NKK88 pKa = 9.18TYY90 pKa = 10.22CSKK93 pKa = 10.91EE94 pKa = 3.72DD95 pKa = 3.34VLFEE99 pKa = 4.48EE100 pKa = 5.74GTFPEE105 pKa = 3.94QGKK108 pKa = 10.28RR109 pKa = 11.84SDD111 pKa = 3.47IEE113 pKa = 3.96VCRR116 pKa = 11.84EE117 pKa = 3.69IVQSGAGMRR126 pKa = 11.84EE127 pKa = 4.28VVNHH131 pKa = 5.56CQSVQGMRR139 pKa = 11.84IAEE142 pKa = 4.32SYY144 pKa = 10.68LKK146 pKa = 10.58YY147 pKa = 10.23HH148 pKa = 6.53EE149 pKa = 5.41KK150 pKa = 8.84PRR152 pKa = 11.84KK153 pKa = 8.33WKK155 pKa = 8.82PTVQWFYY162 pKa = 11.59GEE164 pKa = 4.25SRR166 pKa = 11.84SGKK169 pKa = 7.01TRR171 pKa = 11.84EE172 pKa = 4.02AYY174 pKa = 9.51EE175 pKa = 4.01ILGDD179 pKa = 4.76DD180 pKa = 5.52CYY182 pKa = 11.42TCLNTGKK189 pKa = 9.59WFEE192 pKa = 4.75GYY194 pKa = 9.39DD195 pKa = 3.2AHH197 pKa = 7.01EE198 pKa = 4.67NVLIDD203 pKa = 5.24DD204 pKa = 3.76IRR206 pKa = 11.84KK207 pKa = 9.63SFMPYY212 pKa = 10.56DD213 pKa = 3.91EE214 pKa = 5.32FIKK217 pKa = 11.14LIDD220 pKa = 3.62RR221 pKa = 11.84YY222 pKa = 10.27AFRR225 pKa = 11.84RR226 pKa = 11.84RR227 pKa = 11.84MLRR230 pKa = 11.84KK231 pKa = 9.61FPPVFSKK238 pKa = 10.78KK239 pKa = 10.32DD240 pKa = 3.52NYY242 pKa = 10.8HH243 pKa = 6.61LLLSPRR249 pKa = 11.84RR250 pKa = 11.84CLEE253 pKa = 3.53KK254 pKa = 10.26QGRR257 pKa = 11.84YY258 pKa = 7.91

Molecular weight: 30.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IM87|A0A0C5IM87_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 4 OX=1618255 PE=4 SV=1
MM1 pKa = 7.37YY2 pKa = 8.85MYY4 pKa = 10.65NYY6 pKa = 7.69TTMPYY11 pKa = 10.26KK12 pKa = 10.47EE13 pKa = 4.09GTRR16 pKa = 11.84FARR19 pKa = 11.84KK20 pKa = 7.88SVRR23 pKa = 11.84YY24 pKa = 9.41ARR26 pKa = 11.84RR27 pKa = 11.84GAMARR32 pKa = 11.84YY33 pKa = 8.96VPKK36 pKa = 10.59NKK38 pKa = 9.71KK39 pKa = 9.38GKK41 pKa = 9.44RR42 pKa = 11.84ALNIANVIKK51 pKa = 10.59DD52 pKa = 3.23VALIKK57 pKa = 10.59RR58 pKa = 11.84SLNTEE63 pKa = 3.78KK64 pKa = 10.84KK65 pKa = 10.6VINQTLLGLSANRR78 pKa = 11.84GSSLVQALEE87 pKa = 4.12YY88 pKa = 10.16PVNQGLTDD96 pKa = 3.34KK97 pKa = 10.71TRR99 pKa = 11.84IGSHH103 pKa = 5.76IKK105 pKa = 9.03VTGLNLRR112 pKa = 11.84MRR114 pKa = 11.84LRR116 pKa = 11.84LHH118 pKa = 6.93ASANEE123 pKa = 3.85NVTNKK128 pKa = 9.8LQNVRR133 pKa = 11.84YY134 pKa = 8.23KK135 pKa = 11.03LYY137 pKa = 10.54VIWMKK142 pKa = 10.71DD143 pKa = 3.01ASVLLTPQDD152 pKa = 3.64VLEE155 pKa = 4.5EE156 pKa = 4.53DD157 pKa = 3.47FDD159 pKa = 4.92GNYY162 pKa = 10.3SVSSPFNKK170 pKa = 9.94YY171 pKa = 9.03NYY173 pKa = 10.2KK174 pKa = 10.68NFIITNKK181 pKa = 10.04FEE183 pKa = 4.09GTMKK187 pKa = 9.73FTQGLHH193 pKa = 6.29DD194 pKa = 4.01LRR196 pKa = 11.84ANEE199 pKa = 4.11VEE201 pKa = 3.87EE202 pKa = 3.82FRR204 pKa = 11.84YY205 pKa = 10.32INAHH209 pKa = 6.33KK210 pKa = 10.5NLSLKK215 pKa = 9.84MDD217 pKa = 4.35FRR219 pKa = 11.84SSDD222 pKa = 3.75DD223 pKa = 4.3PILPGVLEE231 pKa = 4.38TYY233 pKa = 10.27KK234 pKa = 10.66PYY236 pKa = 11.21LFLLSDD242 pKa = 3.3ASEE245 pKa = 4.22RR246 pKa = 11.84DD247 pKa = 3.3GDD249 pKa = 3.89EE250 pKa = 5.51KK251 pKa = 10.86IDD253 pKa = 3.74FDD255 pKa = 4.25IMSKK259 pKa = 9.28MFYY262 pKa = 10.67VDD264 pKa = 3.1NN265 pKa = 4.62

Molecular weight: 30.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

523

258

265

261.5

30.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.971 ± 0.446

1.53 ± 0.991

5.354 ± 0.443

6.31 ± 0.91

4.398 ± 0.16

4.971 ± 0.043

2.486 ± 0.633

5.163 ± 0.166

9.369 ± 0.202

8.222 ± 1.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.25 ± 0.339

6.31 ± 1.291

3.442 ± 0.274

3.059 ± 0.515

7.075 ± 0.183

5.927 ± 0.316

5.354 ± 0.046

5.545 ± 0.564

0.956 ± 0.375

6.31 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski