Paenibacillus rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6961 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R1ENB0|A0A1R1ENB0_9BACL Uncharacterized protein OS=Paenibacillus rhizosphaerae OX=297318 GN=BK138_19560 PE=4 SV=1
MM1 pKa = 7.36FVVCKK6 pKa = 10.53DD7 pKa = 3.57HH8 pKa = 7.6VEE10 pKa = 3.96LAIDD14 pKa = 3.67MFVDD18 pKa = 4.63EE19 pKa = 5.7YY20 pKa = 11.26EE21 pKa = 4.41DD22 pKa = 4.32APDD25 pKa = 3.86IVDD28 pKa = 3.83LKK30 pKa = 10.11EE31 pKa = 4.31TEE33 pKa = 4.15FSDD36 pKa = 3.06WDD38 pKa = 3.87PPVQCAHH45 pKa = 6.96CEE47 pKa = 3.92QPAKK51 pKa = 10.55FLVVV55 pKa = 3.43

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R1ENV7|A0A1R1ENV7_9BACL Bifunctional ligase/repressor BirA OS=Paenibacillus rhizosphaerae OX=297318 GN=birA PE=3 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6961

0

6961

2180809

26

2221

313.3

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.376 ± 0.035

0.752 ± 0.008

5.257 ± 0.023

6.629 ± 0.032

4.09 ± 0.022

7.72 ± 0.028

2.177 ± 0.016

6.444 ± 0.029

5.15 ± 0.029

10.033 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.016

3.551 ± 0.022

4.274 ± 0.023

3.923 ± 0.017

5.182 ± 0.028

6.285 ± 0.026

5.296 ± 0.025

7.082 ± 0.022

1.367 ± 0.013

3.501 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski