TM7 phage DolZOral124_53_65

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PI02|A0A2H4PI02_9CAUD Uncharacterized protein OS=TM7 phage DolZOral124_53_65 OX=2053587 GN=CQ476_32 PE=4 SV=1
MM1 pKa = 7.2MGGGIGSIAAYY12 pKa = 9.93GAFSWGQNVIAVGGDD27 pKa = 3.43SSSDD31 pKa = 3.25SHH33 pKa = 6.14GQWWTNASVEE43 pKa = 4.22NPQDD47 pKa = 3.27WTDD50 pKa = 3.53LPKK53 pKa = 10.1NTSEE57 pKa = 4.27WNDD60 pKa = 3.38SATEE64 pKa = 4.15QPAEE68 pKa = 3.79WHH70 pKa = 5.97EE71 pKa = 4.02

Molecular weight: 7.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PHT3|A0A2H4PHT3_9CAUD Uncharacterized protein OS=TM7 phage DolZOral124_53_65 OX=2053587 GN=CQ476_26 PE=4 SV=1
MM1 pKa = 7.12TIEE4 pKa = 4.18VEE6 pKa = 4.18SDD8 pKa = 2.86NTGPQEE14 pKa = 3.68VDD16 pKa = 2.83VRR18 pKa = 11.84TKK20 pKa = 11.05AEE22 pKa = 3.85RR23 pKa = 11.84TRR25 pKa = 11.84DD26 pKa = 3.31KK27 pKa = 11.21LLAKK31 pKa = 10.7DD32 pKa = 3.41PDD34 pKa = 3.24HH35 pKa = 6.64YY36 pKa = 11.21RR37 pKa = 11.84RR38 pKa = 11.84IGHH41 pKa = 6.3NGGLKK46 pKa = 10.13SSSRR50 pKa = 11.84PFKK53 pKa = 10.72DD54 pKa = 3.1PEE56 pKa = 3.98VARR59 pKa = 11.84FAVNMRR65 pKa = 11.84WEE67 pKa = 4.18KK68 pKa = 10.7EE69 pKa = 3.92RR70 pKa = 11.84ALKK73 pKa = 9.84KK74 pKa = 9.96ANRR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 9.35RR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 3.11

Molecular weight: 9.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

11896

30

694

237.9

26.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.65 ± 0.445

0.58 ± 0.105

7.011 ± 0.229

5.968 ± 0.542

3.178 ± 0.197

7.683 ± 0.501

1.69 ± 0.149

5.775 ± 0.273

6.044 ± 0.415

6.969 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.234

4.901 ± 0.234

4.371 ± 0.322

4.548 ± 0.338

5.666 ± 0.287

6.548 ± 0.282

6.481 ± 0.353

6.288 ± 0.317

1.345 ± 0.188

3.875 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski