Chryseobacterium lacus
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2165 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A368MX06|A0A368MX06_9FLAO Type II toxin-antitoxin system RelE/ParE family toxin OS=Chryseobacterium lacus OX=2058346 GN=DQ356_08510 PE=4 SV=1
MM1 pKa = 6.55 KK2 pKa = 9.78 TIYY5 pKa = 10.06 IDD7 pKa = 3.45 FTDD10 pKa = 3.67 IGDD13 pKa = 3.74 VEE15 pKa = 5.01 DD16 pKa = 6.03 FYY18 pKa = 11.88 LQLKK22 pKa = 9.78 EE23 pKa = 4.82 KK24 pKa = 10.17 IQLPEE29 pKa = 3.84 HH30 pKa = 6.61 FGNNLDD36 pKa = 3.75 ALYY39 pKa = 10.75 DD40 pKa = 3.95 VISGHH45 pKa = 7.16 LEE47 pKa = 3.98 LPLHH51 pKa = 6.33 LEE53 pKa = 4.31 FVNMRR58 pKa = 11.84 VDD60 pKa = 3.3 QLEE63 pKa = 4.12 DD64 pKa = 3.73 FEE66 pKa = 5.05 EE67 pKa = 4.16 VLITLEE73 pKa = 3.91 EE74 pKa = 5.11 AEE76 pKa = 4.82 DD77 pKa = 3.89 EE78 pKa = 4.55 VEE80 pKa = 4.8 GFSFSYY86 pKa = 10.82 YY87 pKa = 10.35 LEE89 pKa = 4.23 QYY91 pKa = 10.76 EE92 pKa = 4.52 DD93 pKa = 3.34
Molecular weight: 11.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.821
IPC_protein 3.757
Toseland 3.579
ProMoST 3.846
Dawson 3.732
Bjellqvist 3.923
Wikipedia 3.63
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 3.999
Thurlkill 3.617
EMBOSS 3.643
Sillero 3.872
Patrickios 0.896
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A368MV71|A0A368MV71_9FLAO Integrase (Fragment) OS=Chryseobacterium lacus OX=2058346 GN=DQ356_11150 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPSEE10 pKa = 3.59 RR11 pKa = 11.84 KK12 pKa = 9.33 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 10.03 HH17 pKa = 3.97 GFRR20 pKa = 11.84 EE21 pKa = 4.33 RR22 pKa = 11.84 MSTPNGRR29 pKa = 11.84 RR30 pKa = 11.84 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.98 GRR40 pKa = 11.84 KK41 pKa = 8.71 SLTVSAMRR49 pKa = 11.84 AKK51 pKa = 10.48 RR52 pKa = 3.38
Molecular weight: 6.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 10.774
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.223
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.959
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.11
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2165
0
2165
706491
24
2328
326.3
36.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.59 ± 0.048
0.733 ± 0.016
5.128 ± 0.032
6.936 ± 0.074
5.375 ± 0.044
6.473 ± 0.057
1.918 ± 0.026
7.669 ± 0.046
7.502 ± 0.065
9.062 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.573 ± 0.026
5.801 ± 0.059
3.681 ± 0.033
3.765 ± 0.034
3.778 ± 0.039
6.292 ± 0.041
5.662 ± 0.07
6.093 ± 0.044
1.013 ± 0.017
3.956 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here