Rodent associated circovirus 1
Average proteome isoelectric point is 7.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4MWX5|A0A2H4MWX5_9CIRC ATP-dependent helicase Rep OS=Rodent associated circovirus 1 OX=2560708 PE=3 SV=1
MM1 pKa = 7.4 AQAQILQQRR10 pKa = 11.84 LVKK13 pKa = 10.48 QGARR17 pKa = 11.84 GRR19 pKa = 11.84 WCFTINNYY27 pKa = 7.26 TAEE30 pKa = 4.14 EE31 pKa = 4.02 EE32 pKa = 4.22 QAVIALAGEE41 pKa = 4.34 AKK43 pKa = 10.6 YY44 pKa = 10.04 LICGRR49 pKa = 11.84 EE50 pKa = 3.93 VGEE53 pKa = 4.53 NGTPHH58 pKa = 6.46 LQGFVNLKK66 pKa = 8.66 KK67 pKa = 10.33 KK68 pKa = 10.48 KK69 pKa = 9.97 RR70 pKa = 11.84 FAAMKK75 pKa = 8.46 EE76 pKa = 3.96 ALGGRR81 pKa = 11.84 CHH83 pKa = 7.71 LEE85 pKa = 3.51 PARR88 pKa = 11.84 GDD90 pKa = 3.43 DD91 pKa = 4.68 CSNKK95 pKa = 10.16 DD96 pKa = 3.56 YY97 pKa = 10.78 CSKK100 pKa = 11.16 GGDD103 pKa = 3.21 ILIEE107 pKa = 4.02 EE108 pKa = 4.72 GVPQRR113 pKa = 11.84 EE114 pKa = 4.16 RR115 pKa = 11.84 QRR117 pKa = 11.84 TDD119 pKa = 2.95 LKK121 pKa = 10.64 QACEE125 pKa = 4.05 LVTQGGDD132 pKa = 2.95 IRR134 pKa = 11.84 HH135 pKa = 5.68 VARR138 pKa = 11.84 DD139 pKa = 3.61 YY140 pKa = 11.1 PEE142 pKa = 4.1 VFVRR146 pKa = 11.84 YY147 pKa = 9.36 HH148 pKa = 6.75 RR149 pKa = 11.84 GLLALQLYY157 pKa = 10.01 HH158 pKa = 7.78 PDD160 pKa = 3.2 LCLPRR165 pKa = 11.84 QWKK168 pKa = 8.43 TEE170 pKa = 3.65 VFVYY174 pKa = 10.19 VGPPGVGKK182 pKa = 10.62 SRR184 pKa = 11.84 IVQAKK189 pKa = 10.17 APIGYY194 pKa = 7.81 WKK196 pKa = 10.03 PRR198 pKa = 11.84 GKK200 pKa = 9.03 WWDD203 pKa = 3.98 GYY205 pKa = 10.81 HH206 pKa = 6.46 GNSDD210 pKa = 3.91 VILDD214 pKa = 4.7 DD215 pKa = 4.21 FYY217 pKa = 11.95 GWLPFDD223 pKa = 5.03 DD224 pKa = 4.89 LLRR227 pKa = 11.84 MCDD230 pKa = 3.54 RR231 pKa = 11.84 YY232 pKa = 10.52 PLTVEE237 pKa = 4.33 TKK239 pKa = 10.58 GGTTQFLAKK248 pKa = 10.19 RR249 pKa = 11.84 IFITSNKK256 pKa = 9.5 SPSEE260 pKa = 4.11 WYY262 pKa = 10.28 SDD264 pKa = 3.6 EE265 pKa = 3.98 ITNKK269 pKa = 9.76 DD270 pKa = 3.09 ALYY273 pKa = 10.56 RR274 pKa = 11.84 RR275 pKa = 11.84 LTEE278 pKa = 3.61 LHH280 pKa = 6.29 MWTGTCFEE288 pKa = 4.75 HH289 pKa = 7.1 PPPRR293 pKa = 11.84 VMWNHH298 pKa = 7.32 KK299 pKa = 9.92 INYY302 pKa = 8.23
Molecular weight: 34.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.756
IPC2_protein 7.966
IPC_protein 7.834
Toseland 7.527
ProMoST 8.463
Dawson 8.551
Bjellqvist 8.858
Wikipedia 8.492
Rodwell 8.58
Grimsley 7.512
Solomon 8.639
Lehninger 8.639
Nozaki 9.019
DTASelect 8.595
Thurlkill 8.653
EMBOSS 8.741
Sillero 8.96
Patrickios 4.317
IPC_peptide 8.639
IPC2_peptide 7.805
IPC2.peptide.svr19 7.955
Protein with the highest isoelectric point:
>tr|A0A2H4MWX5|A0A2H4MWX5_9CIRC ATP-dependent helicase Rep OS=Rodent associated circovirus 1 OX=2560708 PE=3 SV=1
MM1 pKa = 7.4 AQAQILQQRR10 pKa = 11.84 LVKK13 pKa = 10.48 QGARR17 pKa = 11.84 GRR19 pKa = 11.84 WCFTINNYY27 pKa = 7.26 TAEE30 pKa = 4.14 EE31 pKa = 4.02 EE32 pKa = 4.22 QAVIALAGEE41 pKa = 4.34 AKK43 pKa = 10.6 YY44 pKa = 10.04 LICGRR49 pKa = 11.84 EE50 pKa = 3.93 VGEE53 pKa = 4.53 NGTPHH58 pKa = 6.46 LQGFVNLKK66 pKa = 8.66 KK67 pKa = 10.33 KK68 pKa = 10.48 KK69 pKa = 9.97 RR70 pKa = 11.84 FAAMKK75 pKa = 8.46 EE76 pKa = 3.96 ALGGRR81 pKa = 11.84 CHH83 pKa = 7.71 LEE85 pKa = 3.51 PARR88 pKa = 11.84 GDD90 pKa = 3.43 DD91 pKa = 4.68 CSNKK95 pKa = 10.16 DD96 pKa = 3.56 YY97 pKa = 10.78 CSKK100 pKa = 11.16 GGDD103 pKa = 3.21 ILIEE107 pKa = 4.02 EE108 pKa = 4.72 GVPQRR113 pKa = 11.84 EE114 pKa = 4.16 RR115 pKa = 11.84 QRR117 pKa = 11.84 TDD119 pKa = 2.95 LKK121 pKa = 10.64 QACEE125 pKa = 4.05 LVTQGGDD132 pKa = 2.95 IRR134 pKa = 11.84 HH135 pKa = 5.68 VARR138 pKa = 11.84 DD139 pKa = 3.61 YY140 pKa = 11.1 PEE142 pKa = 4.1 VFVRR146 pKa = 11.84 YY147 pKa = 9.36 HH148 pKa = 6.75 RR149 pKa = 11.84 GLLALQLYY157 pKa = 10.01 HH158 pKa = 7.78 PDD160 pKa = 3.2 LCLPRR165 pKa = 11.84 QWKK168 pKa = 8.43 TEE170 pKa = 3.65 VFVYY174 pKa = 10.19 VGPPGVGKK182 pKa = 10.62 SRR184 pKa = 11.84 IVQAKK189 pKa = 10.17 APIGYY194 pKa = 7.81 WKK196 pKa = 10.03 PRR198 pKa = 11.84 GKK200 pKa = 9.03 WWDD203 pKa = 3.98 GYY205 pKa = 10.81 HH206 pKa = 6.46 GNSDD210 pKa = 3.91 VILDD214 pKa = 4.7 DD215 pKa = 4.21 FYY217 pKa = 11.95 GWLPFDD223 pKa = 5.03 DD224 pKa = 4.89 LLRR227 pKa = 11.84 MCDD230 pKa = 3.54 RR231 pKa = 11.84 YY232 pKa = 10.52 PLTVEE237 pKa = 4.33 TKK239 pKa = 10.58 GGTTQFLAKK248 pKa = 10.19 RR249 pKa = 11.84 IFITSNKK256 pKa = 9.5 SPSEE260 pKa = 4.11 WYY262 pKa = 10.28 SDD264 pKa = 3.6 EE265 pKa = 3.98 ITNKK269 pKa = 9.76 DD270 pKa = 3.09 ALYY273 pKa = 10.56 RR274 pKa = 11.84 RR275 pKa = 11.84 LTEE278 pKa = 3.61 LHH280 pKa = 6.29 MWTGTCFEE288 pKa = 4.75 HH289 pKa = 7.1 PPPRR293 pKa = 11.84 VMWNHH298 pKa = 7.32 KK299 pKa = 9.92 INYY302 pKa = 8.23
Molecular weight: 34.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.756
IPC2_protein 7.966
IPC_protein 7.834
Toseland 7.527
ProMoST 8.463
Dawson 8.551
Bjellqvist 8.858
Wikipedia 8.492
Rodwell 8.58
Grimsley 7.512
Solomon 8.639
Lehninger 8.639
Nozaki 9.019
DTASelect 8.595
Thurlkill 8.653
EMBOSS 8.741
Sillero 8.96
Patrickios 4.317
IPC_peptide 8.639
IPC2_peptide 7.805
IPC2.peptide.svr19 7.955
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
302
302
302
302.0
34.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.291 ± 0.0
2.98 ± 0.0
5.629 ± 0.0
6.291 ± 0.0
3.311 ± 0.0
8.94 ± 0.0
2.98 ± 0.0
4.636 ± 0.0
6.623 ± 0.0
8.609 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.656 ± 0.0
3.311 ± 0.0
5.298 ± 0.0
4.967 ± 0.0
7.616 ± 0.0
2.649 ± 0.0
4.967 ± 0.0
5.629 ± 0.0
2.98 ± 0.0
4.636 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here