Capybara microvirus Cap3_SP_297
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4I0|A0A4P8W4I0_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_297 OX=2585424 PE=4 SV=1
MM1 pKa = 7.16 TRR3 pKa = 11.84 YY4 pKa = 9.31 FLYY7 pKa = 10.99 LEE9 pKa = 5.26 FPRR12 pKa = 11.84 EE13 pKa = 4.06 MYY15 pKa = 10.77 LDD17 pKa = 4.16 SPSKK21 pKa = 9.58 MRR23 pKa = 11.84 KK24 pKa = 9.23 LFLDD28 pKa = 3.87 FVDD31 pKa = 4.01 YY32 pKa = 11.32 VRR34 pKa = 11.84 LFFINRR40 pKa = 11.84 NFYY43 pKa = 10.97 SFTIDD48 pKa = 3.4 YY49 pKa = 10.14 VRR51 pKa = 11.84 EE52 pKa = 4.31 LILVDD57 pKa = 4.08 VNSSRR62 pKa = 11.84 YY63 pKa = 10.25 DD64 pKa = 3.5 EE65 pKa = 4.41 VDD67 pKa = 3.23 SLLGEE72 pKa = 4.53 LEE74 pKa = 4.56 DD75 pKa = 5.49 SISTTKK81 pKa = 10.2 FDD83 pKa = 3.09 SYY85 pKa = 11.71 YY86 pKa = 11.19 SVFF89 pKa = 3.46
Molecular weight: 10.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.402
IPC2_protein 4.546
IPC_protein 4.418
Toseland 4.228
ProMoST 4.546
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.291
Rodwell 4.24
Grimsley 4.139
Solomon 4.38
Lehninger 4.329
Nozaki 4.495
DTASelect 4.698
Thurlkill 4.266
EMBOSS 4.304
Sillero 4.533
Patrickios 3.35
IPC_peptide 4.38
IPC2_peptide 4.507
IPC2.peptide.svr19 4.494
Protein with the highest isoelectric point:
>tr|A0A4P8W4S1|A0A4P8W4S1_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_297 OX=2585424 PE=4 SV=1
MM1 pKa = 7.96 SILNLLNFFSKK12 pKa = 10.54 FKK14 pKa = 10.65 PFLKK18 pKa = 9.95 KK19 pKa = 10.56 VYY21 pKa = 10.4 SFTPLVLSVIAIFISLSCLRR41 pKa = 11.84 VSRR44 pKa = 11.84 SALEE48 pKa = 4.15 KK49 pKa = 10.34 SSHH52 pKa = 5.58 AVEE55 pKa = 4.02 ICSNYY60 pKa = 10.37 SSDD63 pKa = 3.06 ISSLVDD69 pKa = 3.24 EE70 pKa = 4.49 VRR72 pKa = 11.84 KK73 pKa = 7.51 MTEE76 pKa = 3.89 TIEE79 pKa = 4.5 NRR81 pKa = 11.84 LDD83 pKa = 3.57 EE84 pKa = 4.33 QNEE87 pKa = 4.28 MLSHH91 pKa = 7.07 IYY93 pKa = 10.39 GDD95 pKa = 4.01 YY96 pKa = 10.67 FISIYY101 pKa = 11.23 GNGNKK106 pKa = 9.24 VDD108 pKa = 3.79 GKK110 pKa = 10.68 NFGKK114 pKa = 9.3 TSKK117 pKa = 10.58 KK118 pKa = 10.52
Molecular weight: 13.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.158
IPC2_protein 8.112
IPC_protein 8.17
Toseland 8.609
ProMoST 8.799
Dawson 8.902
Bjellqvist 8.887
Wikipedia 8.96
Rodwell 9.004
Grimsley 8.521
Solomon 9.151
Lehninger 9.136
Nozaki 9.033
DTASelect 8.756
Thurlkill 8.858
EMBOSS 9.092
Sillero 9.077
Patrickios 4.558
IPC_peptide 9.136
IPC2_peptide 7.658
IPC2.peptide.svr19 7.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1973
89
784
394.6
44.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.815 ± 1.142
1.368 ± 0.47
6.842 ± 0.322
4.105 ± 0.473
7.805 ± 0.797
4.866 ± 0.715
1.926 ± 0.604
6.133 ± 0.375
4.764 ± 1.001
8.92 ± 0.497
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.484 ± 0.269
6.386 ± 0.461
4.257 ± 0.73
2.636 ± 0.659
4.308 ± 0.329
11.809 ± 0.926
4.967 ± 0.416
5.981 ± 0.433
0.456 ± 0.084
5.17 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here