Roseburia sp. CAG:45

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2315 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6NDA0|R6NDA0_9FIRM Transcriptional regulator MarR family OS=Roseburia sp. CAG:45 OX=1262947 GN=BN662_01280 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.26KK3 pKa = 10.38KK4 pKa = 10.72LISTLLCVSMVAAMVAGCGSGDD26 pKa = 3.66DD27 pKa = 4.1TKK29 pKa = 11.36DD30 pKa = 3.51ANNDD34 pKa = 3.09ASNTEE39 pKa = 4.03GTADD43 pKa = 4.34ADD45 pKa = 3.83TDD47 pKa = 3.82TDD49 pKa = 3.8SGSDD53 pKa = 3.33STEE56 pKa = 3.75VEE58 pKa = 3.96NTITGDD64 pKa = 3.57PSADD68 pKa = 3.36DD69 pKa = 4.85AFVVWGWNDD78 pKa = 3.8DD79 pKa = 3.35IKK81 pKa = 11.45NILDD85 pKa = 3.66GPFAEE90 pKa = 5.14QYY92 pKa = 10.09PDD94 pKa = 3.12LAKK97 pKa = 10.57RR98 pKa = 11.84IVFVNAGGSDD108 pKa = 4.07YY109 pKa = 10.9YY110 pKa = 8.83QTKK113 pKa = 9.63IDD115 pKa = 5.09EE116 pKa = 4.66ILDD119 pKa = 4.14DD120 pKa = 5.29PDD122 pKa = 4.42NEE124 pKa = 4.8LYY126 pKa = 9.9PDD128 pKa = 4.09LMGLEE133 pKa = 3.98VDD135 pKa = 4.07YY136 pKa = 11.22VLKK139 pKa = 10.78YY140 pKa = 11.07VNSDD144 pKa = 2.6WLTSVGDD151 pKa = 4.07CGITADD157 pKa = 5.17DD158 pKa = 4.17YY159 pKa = 12.05ANQYY163 pKa = 9.89QYY165 pKa = 11.82NLDD168 pKa = 3.83LGTVSGEE175 pKa = 4.28DD176 pKa = 3.12SSDD179 pKa = 3.56ANNVKK184 pKa = 10.48ALFWQATPGCFQLRR198 pKa = 11.84ADD200 pKa = 4.07LCEE203 pKa = 4.77KK204 pKa = 10.89YY205 pKa = 10.79LGTTDD210 pKa = 3.76PAEE213 pKa = 4.31LSTMFSTWDD222 pKa = 4.15GILEE226 pKa = 4.25AARR229 pKa = 11.84KK230 pKa = 10.03VNDD233 pKa = 3.89ASSGKK238 pKa = 10.12CKK240 pKa = 10.37LFSGYY245 pKa = 9.58DD246 pKa = 2.98EE247 pKa = 4.7CFRR250 pKa = 11.84VLSNSRR256 pKa = 11.84ATGWYY261 pKa = 10.02GDD263 pKa = 4.36DD264 pKa = 5.14DD265 pKa = 5.61VITVDD270 pKa = 5.47DD271 pKa = 4.52NMTEE275 pKa = 4.05YY276 pKa = 10.42MDD278 pKa = 4.17LAKK281 pKa = 10.42TMVDD285 pKa = 2.88EE286 pKa = 4.56GLTYY290 pKa = 9.62EE291 pKa = 4.13TDD293 pKa = 2.78QWSTDD298 pKa = 2.44WYY300 pKa = 10.94ANMEE304 pKa = 4.31GDD306 pKa = 4.65GEE308 pKa = 4.45TSNAAVAYY316 pKa = 8.55CGCPWFTYY324 pKa = 9.57WSLKK328 pKa = 9.1DD329 pKa = 3.13TWKK332 pKa = 11.22GNTILVNAPEE342 pKa = 3.88QFYY345 pKa = 10.47WGGTGLAATANCADD359 pKa = 4.14KK360 pKa = 11.13EE361 pKa = 4.26MAGTIIKK368 pKa = 10.6AFTCDD373 pKa = 2.84TDD375 pKa = 4.49FMVSINAKK383 pKa = 10.42NSDD386 pKa = 3.39FVNNKK391 pKa = 9.03EE392 pKa = 4.02AVKK395 pKa = 10.39KK396 pKa = 10.39ISEE399 pKa = 4.28AGATCDD405 pKa = 3.72YY406 pKa = 10.32LYY408 pKa = 10.82EE409 pKa = 4.43AAGQDD414 pKa = 2.66IMAFYY419 pKa = 11.04LPMADD424 pKa = 5.11SISAKK429 pKa = 10.3NATAEE434 pKa = 4.06DD435 pKa = 3.92QNINSAWSTQVKK447 pKa = 9.92EE448 pKa = 3.98YY449 pKa = 11.05AAGNKK454 pKa = 10.24DD455 pKa = 3.71KK456 pKa = 10.39DD457 pKa = 3.54TAIADD462 pKa = 4.19FKK464 pKa = 11.35SAVHH468 pKa = 7.06DD469 pKa = 3.94SYY471 pKa = 12.12SYY473 pKa = 11.45LKK475 pKa = 11.11AEE477 pKa = 4.2

Molecular weight: 52.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6NZ56|R6NZ56_9FIRM Chorismate synthase OS=Roseburia sp. CAG:45 OX=1262947 GN=aroC PE=3 SV=1
MM1 pKa = 7.64HH2 pKa = 7.44KK3 pKa = 10.88GKK5 pKa = 8.15MTFQPKK11 pKa = 8.98NRR13 pKa = 11.84QRR15 pKa = 11.84NKK17 pKa = 7.97VHH19 pKa = 6.65GFRR22 pKa = 11.84KK23 pKa = 9.94RR24 pKa = 11.84MSTAGGRR31 pKa = 11.84KK32 pKa = 8.62VLAARR37 pKa = 11.84RR38 pKa = 11.84LKK40 pKa = 10.61GRR42 pKa = 11.84KK43 pKa = 8.87KK44 pKa = 10.59LSAA47 pKa = 3.95

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2315

0

2315

734199

29

2707

317.1

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.622 ± 0.049

1.404 ± 0.021

5.682 ± 0.047

8.047 ± 0.057

3.972 ± 0.042

6.967 ± 0.046

1.768 ± 0.025

7.54 ± 0.052

7.034 ± 0.045

8.824 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.201 ± 0.028

4.481 ± 0.038

3.062 ± 0.029

3.257 ± 0.032

4.061 ± 0.038

5.623 ± 0.046

5.48 ± 0.041

6.919 ± 0.045

0.789 ± 0.018

4.266 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski