Microvirgula aerodenitrificans

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S0P9I2|A0A2S0P9I2_9NEIS Cytochrome c oxidase assembly protein CtaG OS=Microvirgula aerodenitrificans OX=57480 GN=DAI18_07740 PE=3 SV=1
MM1 pKa = 7.62SATLLDD7 pKa = 3.94LAKK10 pKa = 10.63SFVDD14 pKa = 4.11GEE16 pKa = 4.21ISASDD21 pKa = 3.62FSDD24 pKa = 3.06IYY26 pKa = 10.28INLWRR31 pKa = 11.84VEE33 pKa = 4.09RR34 pKa = 11.84DD35 pKa = 2.86SGIILNDD42 pKa = 3.38EE43 pKa = 4.17PHH45 pKa = 7.05LSEE48 pKa = 4.48CLSSIFCAADD58 pKa = 3.7MYY60 pKa = 11.28CADD63 pKa = 3.93DD64 pKa = 3.77EE65 pKa = 4.43TRR67 pKa = 11.84EE68 pKa = 4.1VYY70 pKa = 10.17EE71 pKa = 5.01FNDD74 pKa = 3.51SQLKK78 pKa = 10.66NEE80 pKa = 4.31ILSIIDD86 pKa = 3.17RR87 pKa = 11.84CLL89 pKa = 3.05

Molecular weight: 10.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S0P8T9|A0A2S0P8T9_9NEIS EscG/YscG/SsaH family type III secretion system needle protein co-chaperone OS=Microvirgula aerodenitrificans OX=57480 GN=DAI18_06945 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVVKK11 pKa = 10.69RR12 pKa = 11.84KK13 pKa = 7.73RR14 pKa = 11.84THH16 pKa = 5.59GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.54TRR25 pKa = 11.84GGQSVIGARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3570

0

3570

1163100

30

4445

325.8

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.239 ± 0.066

0.997 ± 0.014

5.857 ± 0.035

4.921 ± 0.034

3.466 ± 0.028

8.304 ± 0.045

2.277 ± 0.02

4.625 ± 0.034

2.874 ± 0.036

11.305 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.02

2.673 ± 0.03

5.106 ± 0.036

3.827 ± 0.029

7.317 ± 0.047

5.582 ± 0.035

5.172 ± 0.033

7.326 ± 0.037

1.389 ± 0.019

2.402 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski