Listeria phage PSU-VKH-LP041

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UUT6|A0A2U8UUT6_9CAUD Putative portal protein OS=Listeria phage PSU-VKH-LP041 OX=2202248 PE=4 SV=1
MM1 pKa = 7.81EE2 pKa = 5.94IYY4 pKa = 9.28ITTNNEE10 pKa = 3.52GFLTGYY16 pKa = 7.94STSEE20 pKa = 3.86CTPGKK25 pKa = 10.46KK26 pKa = 8.89IDD28 pKa = 3.7IDD30 pKa = 3.75EE31 pKa = 4.44NDD33 pKa = 3.43AFFQRR38 pKa = 11.84GFASYY43 pKa = 10.25KK44 pKa = 10.08YY45 pKa = 9.95KK46 pKa = 10.9ANQLIFDD53 pKa = 4.1EE54 pKa = 4.56NKK56 pKa = 9.91EE57 pKa = 4.44KK58 pKa = 10.74EE59 pKa = 4.16FQYY62 pKa = 11.23EE63 pKa = 4.03KK64 pKa = 10.24TLQEE68 pKa = 4.04AMLSTEE74 pKa = 4.22EE75 pKa = 4.16QLLTTQEE82 pKa = 4.23ALVEE86 pKa = 4.21LYY88 pKa = 9.68EE89 pKa = 4.24QNTKK93 pKa = 10.75LEE95 pKa = 4.03QQLIDD100 pKa = 3.61TQLAMVDD107 pKa = 3.47MYY109 pKa = 10.94EE110 pKa = 4.31ANLL113 pKa = 3.6

Molecular weight: 13.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UV77|A0A2U8UV77_9CAUD Uncharacterized protein OS=Listeria phage PSU-VKH-LP041 OX=2202248 PE=4 SV=1
MM1 pKa = 6.82YY2 pKa = 10.44VKK4 pKa = 10.39NRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 8.02VKK11 pKa = 10.33RR12 pKa = 11.84KK13 pKa = 8.46ITIKK17 pKa = 10.49QNSKK21 pKa = 10.83RR22 pKa = 11.84EE23 pKa = 3.98LDD25 pKa = 3.4NAVYY29 pKa = 10.19QFILANPNVEE39 pKa = 5.14FKK41 pKa = 11.21HH42 pKa = 5.38EE43 pKa = 3.97MRR45 pKa = 11.84LKK47 pKa = 9.54KK48 pKa = 8.57TWRR51 pKa = 11.84GKK53 pKa = 9.07WKK55 pKa = 9.61WILKK59 pKa = 10.04IEE61 pKa = 4.04ITKK64 pKa = 10.25VV65 pKa = 2.9

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

15184

41

1569

187.5

21.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.093 ± 0.511

0.757 ± 0.128

5.842 ± 0.31

8.509 ± 0.473

3.892 ± 0.157

5.736 ± 0.56

1.475 ± 0.123

7.514 ± 0.276

9.444 ± 0.411

7.943 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.134

6.29 ± 0.226

2.766 ± 0.176

3.392 ± 0.179

4.116 ± 0.243

6.599 ± 0.266

5.73 ± 0.237

6.072 ± 0.296

1.06 ± 0.114

3.471 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski