TM7 phylum sp. oral taxon 352
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 703 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A563CM93|A0A563CM93_9BACT Uncharacterized protein OS=TM7 phylum sp. oral taxon 352 OX=713054 GN=EUA74_03565 PE=4 SV=1
MM1 pKa = 7.44 NSNNTSLGISLRR13 pKa = 11.84 TMVFTISACAVFVLTLGTTIGAIVCSTALLVAAQALTIPLGWVYY57 pKa = 10.47 FSSTGKK63 pKa = 10.16 IPTVALKK70 pKa = 9.04 VTAILGTVVYY80 pKa = 10.29 VALLASNIDD89 pKa = 3.6 TLITDD94 pKa = 3.72 WGDD97 pKa = 2.85 LTTAFNVVIDD107 pKa = 4.07 GEE109 pKa = 4.66 GWADD113 pKa = 3.4 ALAFTLFMCTLPVVTAVIAPVPPEE137 pKa = 4.01 RR138 pKa = 11.84 DD139 pKa = 3.2 DD140 pKa = 4.01 YY141 pKa = 12.29 GNDD144 pKa = 4.16 DD145 pKa = 3.69 EE146 pKa = 5.91 DD147 pKa = 3.67
Molecular weight: 15.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.694
IPC_protein 3.643
Toseland 3.427
ProMoST 3.77
Dawson 3.668
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.478
Grimsley 3.338
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.77
Patrickios 0.693
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.775
Protein with the highest isoelectric point:
>tr|A0A563CB94|A0A563CB94_9BACT Single-stranded-DNA-specific exonuclease RecJ OS=TM7 phylum sp. oral taxon 352 OX=713054 GN=recJ PE=3 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.06 RR4 pKa = 11.84 TFQPHH9 pKa = 4.1 TRR11 pKa = 11.84 HH12 pKa = 5.99 RR13 pKa = 11.84 AKK15 pKa = 9.66 THH17 pKa = 5.06 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 VSTKK26 pKa = 9.98 AGRR29 pKa = 11.84 MVLKK33 pKa = 10.41 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 LKK38 pKa = 10.74 GRR40 pKa = 11.84 AKK42 pKa = 10.19 IAII45 pKa = 4.11
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
703
0
703
207660
14
1277
295.4
32.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.164 ± 0.098
0.645 ± 0.027
5.958 ± 0.082
6.502 ± 0.102
3.798 ± 0.062
6.789 ± 0.084
1.7 ± 0.037
7.368 ± 0.082
7.005 ± 0.109
9.153 ± 0.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.414 ± 0.039
4.591 ± 0.061
3.781 ± 0.058
3.603 ± 0.056
5.107 ± 0.071
6.671 ± 0.086
5.435 ± 0.067
7.112 ± 0.082
1.048 ± 0.04
3.155 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here