Lactobacillus prophage Lj771

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9D9K7|A9D9K7_9CAUD Uncharacterized protein OS=Lactobacillus prophage Lj771 OX=139871 GN=LJ771_008 PE=4 SV=1
MM1 pKa = 8.13VEE3 pKa = 4.25IMTPAQAAAFRR14 pKa = 11.84EE15 pKa = 4.03QRR17 pKa = 11.84LKK19 pKa = 11.04EE20 pKa = 4.06EE21 pKa = 3.72QRR23 pKa = 11.84KK24 pKa = 7.98YY25 pKa = 10.94AEE27 pKa = 4.54RR28 pKa = 11.84GISTALEE35 pKa = 3.99GWTLVTIGDD44 pKa = 4.2SDD46 pKa = 4.39CNYY49 pKa = 10.04YY50 pKa = 10.41SYY52 pKa = 11.57KK53 pKa = 10.54HH54 pKa = 5.85FVVTQIFGMGIDD66 pKa = 3.87NYY68 pKa = 10.16ISKK71 pKa = 9.05TGWDD75 pKa = 3.58EE76 pKa = 4.2KK77 pKa = 11.32EE78 pKa = 4.29LIEE81 pKa = 6.01FLATDD86 pKa = 4.75DD87 pKa = 5.73DD88 pKa = 4.72PNEE91 pKa = 4.68DD92 pKa = 3.26PWKK95 pKa = 10.28EE96 pKa = 3.64DD97 pKa = 3.05VINYY101 pKa = 9.55FDD103 pKa = 5.06GIEE106 pKa = 3.92GNYY109 pKa = 10.01

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9D9U4|A9D9U4_9CAUD Uncharacterized protein OS=Lactobacillus prophage Lj771 OX=139871 GN=LJ771_037 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84LGHH5 pKa = 6.59VDD7 pKa = 3.59DD8 pKa = 5.48AQFQQFASRR17 pKa = 11.84VRR19 pKa = 11.84QKK21 pKa = 10.51IDD23 pKa = 2.77SGYY26 pKa = 10.69VKK28 pKa = 10.54QEE30 pKa = 3.82LGKK33 pKa = 9.29SSKK36 pKa = 10.69RR37 pKa = 11.84IGTQSLRR44 pKa = 11.84ILKK47 pKa = 10.5ANTPVKK53 pKa = 10.25QGNLRR58 pKa = 11.84RR59 pKa = 11.84SWTAEE64 pKa = 3.71GPSYY68 pKa = 10.83GGSGWTIKK76 pKa = 10.58LINNAEE82 pKa = 4.04YY83 pKa = 10.77ASWVEE88 pKa = 4.07SGHH91 pKa = 5.66RR92 pKa = 11.84QTPGRR97 pKa = 11.84YY98 pKa = 8.52VPVLKK103 pKa = 10.59KK104 pKa = 10.63CLVRR108 pKa = 11.84DD109 pKa = 3.88WVPGQFYY116 pKa = 9.23MKK118 pKa = 10.4KK119 pKa = 10.09SIPQIQRR126 pKa = 11.84QLPQLVTEE134 pKa = 4.78GLWGLKK140 pKa = 10.3DD141 pKa = 3.58LFEE144 pKa = 4.45

Molecular weight: 16.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12149

69

1138

216.9

24.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.523 ± 0.77

0.56 ± 0.093

7.21 ± 0.393

6.075 ± 0.567

3.613 ± 0.175

6.601 ± 0.594

1.358 ± 0.151

6.322 ± 0.239

8.577 ± 0.463

8.569 ± 0.403

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.289

6.05 ± 0.492

3.029 ± 0.194

4.116 ± 0.203

3.943 ± 0.335

6.033 ± 0.227

5.984 ± 0.403

6.651 ± 0.364

1.424 ± 0.166

3.885 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski