Amino acid dipepetide frequency for Ustilago hordei (strain Uh4875-4) (Barley covered smut fungus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.894AlaAla: 12.894 ± 0.102	0.963AlaCys: 0.963 ± 0.018	4.995AlaAsp: 4.995 ± 0.046	5.736AlaGlu: 5.736 ± 0.048	3.158AlaPhe: 3.158 ± 0.034	6.875AlaGly: 6.875 ± 0.05	2.048AlaHis: 2.048 ± 0.026	4.048AlaIle: 4.048 ± 0.035	4.998AlaLys: 4.998 ± 0.044	8.256AlaLeu: 8.256 ± 0.057	2.004AlaMet: 2.004 ± 0.024	3.546AlaAsn: 3.546 ± 0.035	5.764AlaPro: 5.764 ± 0.061	4.303AlaGln: 4.303 ± 0.04	5.788AlaArg: 5.788 ± 0.053	11.4AlaSer: 11.4 ± 0.091	6.272AlaThr: 6.272 ± 0.049	5.368AlaVal: 5.368 ± 0.043	1.056AlaTrp: 1.056 ± 0.018	2.069AlaTyr: 2.069 ± 0.025	0.0AlaXaa: 0.0 ± 0.0
Cys	0.819CysAla: 0.819 ± 0.015	0.203CysCys: 0.203 ± 0.007	0.519CysAsp: 0.519 ± 0.012	0.452CysGlu: 0.452 ± 0.012	0.444CysPhe: 0.444 ± 0.012	0.698CysGly: 0.698 ± 0.015	0.302CysHis: 0.302 ± 0.009	0.613CysIle: 0.613 ± 0.014	0.527CysLys: 0.527 ± 0.014	1.058CysLeu: 1.058 ± 0.02	0.228CysMet: 0.228 ± 0.007	0.41CysAsn: 0.41 ± 0.01	0.551CysPro: 0.551 ± 0.013	0.391CysGln: 0.391 ± 0.01	0.621CysArg: 0.621 ± 0.014	0.973CysSer: 0.973 ± 0.017	0.673CysThr: 0.673 ± 0.014	0.608CysVal: 0.608 ± 0.013	0.169CysTrp: 0.169 ± 0.006	0.286CysTyr: 0.286 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	5.832AspAla: 5.832 ± 0.04	0.515AspCys: 0.515 ± 0.012	4.533AspAsp: 4.533 ± 0.064	4.398AspGlu: 4.398 ± 0.048	1.803AspPhe: 1.803 ± 0.024	3.919AspGly: 3.919 ± 0.038	1.235AspHis: 1.235 ± 0.02	2.214AspIle: 2.214 ± 0.022	2.336AspLys: 2.336 ± 0.03	5.052AspLeu: 5.052 ± 0.035	1.076AspMet: 1.076 ± 0.016	1.657AspAsn: 1.657 ± 0.024	3.35AspPro: 3.35 ± 0.031	2.164AspGln: 2.164 ± 0.03	3.076AspArg: 3.076 ± 0.035	4.405AspSer: 4.405 ± 0.034	2.727AspThr: 2.727 ± 0.025	3.255AspVal: 3.255 ± 0.029	0.762AspTrp: 0.762 ± 0.014	1.248AspTyr: 1.248 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	6.219GluAla: 6.219 ± 0.049	0.503GluCys: 0.503 ± 0.012	3.791GluAsp: 3.791 ± 0.042	5.727GluGlu: 5.727 ± 0.073	1.463GluPhe: 1.463 ± 0.022	3.808GluGly: 3.808 ± 0.044	1.333GluHis: 1.333 ± 0.019	2.372GluIle: 2.372 ± 0.024	3.299GluLys: 3.299 ± 0.037	5.086GluLeu: 5.086 ± 0.045	1.351GluMet: 1.351 ± 0.019	1.646GluAsn: 1.646 ± 0.021	2.465GluPro: 2.465 ± 0.027	2.925GluGln: 2.925 ± 0.028	3.924GluArg: 3.924 ± 0.038	4.036GluSer: 4.036 ± 0.037	2.98GluThr: 2.98 ± 0.027	3.197GluVal: 3.197 ± 0.032	0.751GluTrp: 0.751 ± 0.016	1.277GluTyr: 1.277 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.136PheAla: 3.136 ± 0.026	0.425PheCys: 0.425 ± 0.01	2.138PheAsp: 2.138 ± 0.023	1.817PheGlu: 1.817 ± 0.022	1.287PhePhe: 1.287 ± 0.02	2.725PheGly: 2.725 ± 0.038	0.779PheHis: 0.779 ± 0.014	1.34PheIle: 1.34 ± 0.023	1.282PheLys: 1.282 ± 0.018	2.862PheLeu: 2.862 ± 0.036	0.592PheMet: 0.592 ± 0.014	1.212PheAsn: 1.212 ± 0.022	1.532PhePro: 1.532 ± 0.022	1.142PheGln: 1.142 ± 0.015	1.727PheArg: 1.727 ± 0.019	2.879PheSer: 2.879 ± 0.03	1.759PheThr: 1.759 ± 0.02	2.064PheVal: 2.064 ± 0.024	0.465PheTrp: 0.465 ± 0.012	0.875PheTyr: 0.875 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	6.338GlyAla: 6.338 ± 0.054	0.701GlyCys: 0.701 ± 0.017	3.314GlyAsp: 3.314 ± 0.034	3.831GlyGlu: 3.831 ± 0.043	2.419GlyPhe: 2.419 ± 0.029	6.801GlyGly: 6.801 ± 0.085	1.584GlyHis: 1.584 ± 0.02	2.847GlyIle: 2.847 ± 0.032	3.858GlyLys: 3.858 ± 0.041	5.859GlyLeu: 5.859 ± 0.041	1.529GlyMet: 1.529 ± 0.025	2.352GlyAsn: 2.352 ± 0.029	3.238GlyPro: 3.238 ± 0.04	2.681GlyGln: 2.681 ± 0.028	4.163GlyArg: 4.163 ± 0.038	6.782GlySer: 6.782 ± 0.061	3.699GlyThr: 3.699 ± 0.042	3.867GlyVal: 3.867 ± 0.036	1.028GlyTrp: 1.028 ± 0.017	1.735GlyTyr: 1.735 ± 0.028	0.0GlyXaa: 0.0 ± 0.0
His	2.156HisAla: 2.156 ± 0.024	0.3HisCys: 0.3 ± 0.01	1.278HisAsp: 1.278 ± 0.019	1.132HisGlu: 1.132 ± 0.017	0.863HisPhe: 0.863 ± 0.016	1.55HisGly: 1.55 ± 0.024	1.118HisHis: 1.118 ± 0.026	1.06HisIle: 1.06 ± 0.018	0.94HisLys: 0.94 ± 0.015	2.542HisLeu: 2.542 ± 0.03	0.477HisMet: 0.477 ± 0.011	0.835HisAsn: 0.835 ± 0.013	1.852HisPro: 1.852 ± 0.028	1.192HisGln: 1.192 ± 0.023	1.604HisArg: 1.604 ± 0.023	2.18HisSer: 2.18 ± 0.027	1.327HisThr: 1.327 ± 0.019	1.367HisVal: 1.367 ± 0.017	0.296HisTrp: 0.296 ± 0.008	0.614HisTyr: 0.614 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.031IleAla: 4.031 ± 0.035	0.577IleCys: 0.577 ± 0.013	2.692IleAsp: 2.692 ± 0.026	2.472IleGlu: 2.472 ± 0.028	1.498IlePhe: 1.498 ± 0.023	2.685IleGly: 2.685 ± 0.03	1.054IleHis: 1.054 ± 0.017	1.737IleIle: 1.737 ± 0.026	2.07IleLys: 2.07 ± 0.026	3.747IleLeu: 3.747 ± 0.036	0.759IleMet: 0.759 ± 0.016	1.54IleAsn: 1.54 ± 0.019	2.469IlePro: 2.469 ± 0.029	1.729IleGln: 1.729 ± 0.021	2.56IleArg: 2.56 ± 0.025	3.567IleSer: 3.567 ± 0.029	2.34IleThr: 2.34 ± 0.023	2.608IleVal: 2.608 ± 0.028	0.562IleTrp: 0.562 ± 0.013	1.041IleTyr: 1.041 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	5.053LysAla: 5.053 ± 0.044	0.45LysCys: 0.45 ± 0.011	2.672LysAsp: 2.672 ± 0.032	3.258LysGlu: 3.258 ± 0.041	1.181LysPhe: 1.181 ± 0.021	3.197LysGly: 3.197 ± 0.033	1.164LysHis: 1.164 ± 0.017	1.902LysIle: 1.902 ± 0.023	3.274LysLys: 3.274 ± 0.044	4.311LysLeu: 4.311 ± 0.036	1.028LysMet: 1.028 ± 0.016	1.449LysAsn: 1.449 ± 0.023	2.703LysPro: 2.703 ± 0.033	2.307LysGln: 2.307 ± 0.027	3.473LysArg: 3.473 ± 0.036	3.596LysSer: 3.596 ± 0.039	2.618LysThr: 2.618 ± 0.027	2.935LysVal: 2.935 ± 0.03	0.577LysTrp: 0.577 ± 0.012	1.043LysTyr: 1.043 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	8.834LeuAla: 8.834 ± 0.056	1.078LeuCys: 1.078 ± 0.019	5.246LeuAsp: 5.246 ± 0.039	5.123LeuGlu: 5.123 ± 0.046	3.039LeuPhe: 3.039 ± 0.036	5.716LeuGly: 5.716 ± 0.039	2.277LeuHis: 2.277 ± 0.029	3.67LeuIle: 3.67 ± 0.041	3.913LeuLys: 3.913 ± 0.034	8.474LeuLeu: 8.474 ± 0.06	1.603LeuMet: 1.603 ± 0.019	3.016LeuAsn: 3.016 ± 0.031	5.795LeuPro: 5.795 ± 0.044	3.982LeuGln: 3.982 ± 0.039	5.558LeuArg: 5.558 ± 0.046	8.16LeuSer: 8.16 ± 0.05	4.899LeuThr: 4.899 ± 0.041	5.23LeuVal: 5.23 ± 0.039	0.982LeuTrp: 0.982 ± 0.017	1.951LeuTyr: 1.951 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	1.975MetAla: 1.975 ± 0.023	0.194MetCys: 0.194 ± 0.007	1.167MetAsp: 1.167 ± 0.019	1.083MetGlu: 1.083 ± 0.016	0.58MetPhe: 0.58 ± 0.013	1.333MetGly: 1.333 ± 0.022	0.505MetHis: 0.505 ± 0.012	0.817MetIle: 0.817 ± 0.015	0.755MetLys: 0.755 ± 0.012	1.92MetLeu: 1.92 ± 0.024	0.505MetMet: 0.505 ± 0.011	0.616MetAsn: 0.616 ± 0.015	1.304MetPro: 1.304 ± 0.018	0.99MetGln: 0.99 ± 0.019	1.162MetArg: 1.162 ± 0.017	1.896MetSer: 1.896 ± 0.024	1.131MetThr: 1.131 ± 0.017	1.205MetVal: 1.205 ± 0.016	0.207MetTrp: 0.207 ± 0.007	0.42MetTyr: 0.42 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.673AsnAla: 3.673 ± 0.034	0.341AsnCys: 0.341 ± 0.009	1.885AsnAsp: 1.885 ± 0.024	1.74AsnGlu: 1.74 ± 0.021	1.103AsnPhe: 1.103 ± 0.02	2.92AsnGly: 2.92 ± 0.031	0.798AsnHis: 0.798 ± 0.013	1.476AsnIle: 1.476 ± 0.025	1.682AsnLys: 1.682 ± 0.027	3.116AsnLeu: 3.116 ± 0.03	0.701AsnMet: 0.701 ± 0.014	1.502AsnAsn: 1.502 ± 0.026	2.199AsnPro: 2.199 ± 0.027	1.326AsnGln: 1.326 ± 0.022	1.9AsnArg: 1.9 ± 0.023	2.972AsnSer: 2.972 ± 0.032	2.115AsnThr: 2.115 ± 0.022	2.081AsnVal: 2.081 ± 0.025	0.437AsnTrp: 0.437 ± 0.01	0.788AsnTyr: 0.788 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	6.319ProAla: 6.319 ± 0.065	0.535ProCys: 0.535 ± 0.014	2.755ProAsp: 2.755 ± 0.027	3.019ProGlu: 3.019 ± 0.03	1.928ProPhe: 1.928 ± 0.022	3.576ProGly: 3.576 ± 0.044	1.443ProHis: 1.443 ± 0.021	2.376ProIle: 2.376 ± 0.024	2.5ProLys: 2.5 ± 0.029	4.998ProLeu: 4.998 ± 0.036	1.008ProMet: 1.008 ± 0.017	2.152ProAsn: 2.152 ± 0.024	5.342ProPro: 5.342 ± 0.076	2.465ProGln: 2.465 ± 0.038	3.282ProArg: 3.282 ± 0.033	7.809ProSer: 7.809 ± 0.068	4.448ProThr: 4.448 ± 0.039	3.105ProVal: 3.105 ± 0.031	0.588ProTrp: 0.588 ± 0.013	1.324ProTyr: 1.324 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	4.507GlnAla: 4.507 ± 0.042	0.403GlnCys: 0.403 ± 0.012	2.342GlnAsp: 2.342 ± 0.022	2.49GlnGlu: 2.49 ± 0.028	1.055GlnPhe: 1.055 ± 0.018	2.714GlnGly: 2.714 ± 0.034	1.466GlnHis: 1.466 ± 0.023	1.712GlnIle: 1.712 ± 0.02	1.876GlnLys: 1.876 ± 0.024	3.95GlnLeu: 3.95 ± 0.04	0.924GlnMet: 0.924 ± 0.016	1.41GlnAsn: 1.41 ± 0.02	2.885GlnPro: 2.885 ± 0.033	4.029GlnGln: 4.029 ± 0.098	2.99GlnArg: 2.99 ± 0.031	3.609GlnSer: 3.609 ± 0.035	2.316GlnThr: 2.316 ± 0.026	2.461GlnVal: 2.461 ± 0.025	0.442GlnTrp: 0.442 ± 0.01	0.958GlnTyr: 0.958 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	5.344ArgAla: 5.344 ± 0.048	0.658ArgCys: 0.658 ± 0.015	3.111ArgAsp: 3.111 ± 0.031	3.488ArgGlu: 3.488 ± 0.035	2.055ArgPhe: 2.055 ± 0.022	3.76ArgGly: 3.76 ± 0.043	1.529ArgHis: 1.529 ± 0.022	2.775ArgIle: 2.775 ± 0.028	3.542ArgLys: 3.542 ± 0.039	5.41ArgLeu: 5.41 ± 0.049	1.307ArgMet: 1.307 ± 0.018	2.2ArgAsn: 2.2 ± 0.022	3.593ArgPro: 3.593 ± 0.04	2.811ArgGln: 2.811 ± 0.026	5.222ArgArg: 5.222 ± 0.056	6.174ArgSer: 6.174 ± 0.05	3.386ArgThr: 3.386 ± 0.035	3.034ArgVal: 3.034 ± 0.03	0.771ArgTrp: 0.771 ± 0.015	1.433ArgTyr: 1.433 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	9.887SerAla: 9.887 ± 0.075	0.878SerCys: 0.878 ± 0.016	4.773SerAsp: 4.773 ± 0.04	4.184SerGlu: 4.184 ± 0.039	3.082SerPhe: 3.082 ± 0.032	6.284SerGly: 6.284 ± 0.056	2.444SerHis: 2.444 ± 0.028	4.152SerIle: 4.152 ± 0.035	4.3SerLys: 4.3 ± 0.044	8.031SerLeu: 8.031 ± 0.056	1.819SerMet: 1.819 ± 0.021	3.827SerAsn: 3.827 ± 0.035	6.3SerPro: 6.3 ± 0.076	3.905SerGln: 3.905 ± 0.035	5.857SerArg: 5.857 ± 0.06	14.261SerSer: 14.261 ± 0.129	7.393SerThr: 7.393 ± 0.064	4.702SerVal: 4.702 ± 0.037	0.947SerTrp: 0.947 ± 0.017	1.952SerTyr: 1.952 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	6.122ThrAla: 6.122 ± 0.044	0.701ThrCys: 0.701 ± 0.015	2.727ThrAsp: 2.727 ± 0.027	2.713ThrGlu: 2.713 ± 0.029	1.953ThrPhe: 1.953 ± 0.025	3.789ThrGly: 3.789 ± 0.034	1.322ThrHis: 1.322 ± 0.019	2.628ThrIle: 2.628 ± 0.031	2.599ThrLys: 2.599 ± 0.027	5.373ThrLeu: 5.373 ± 0.036	1.048ThrMet: 1.048 ± 0.018	2.104ThrAsn: 2.104 ± 0.025	4.608ThrPro: 4.608 ± 0.048	2.242ThrGln: 2.242 ± 0.024	3.178ThrArg: 3.178 ± 0.032	6.821ThrSer: 6.821 ± 0.049	4.33ThrThr: 4.33 ± 0.044	3.154ThrVal: 3.154 ± 0.031	0.69ThrTrp: 0.69 ± 0.014	1.238ThrTyr: 1.238 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	5.48ValAla: 5.48 ± 0.04	0.645ValCys: 0.645 ± 0.013	3.482ValAsp: 3.482 ± 0.031	3.666ValGlu: 3.666 ± 0.032	1.865ValPhe: 1.865 ± 0.025	3.895ValGly: 3.895 ± 0.038	1.299ValHis: 1.299 ± 0.018	2.323ValIle: 2.323 ± 0.028	2.829ValLys: 2.829 ± 0.03	5.085ValLeu: 5.085 ± 0.043	1.089ValMet: 1.089 ± 0.016	1.875ValAsn: 1.875 ± 0.024	3.257ValPro: 3.257 ± 0.029	2.452ValGln: 2.452 ± 0.026	3.37ValArg: 3.37 ± 0.031	4.582ValSer: 4.582 ± 0.036	2.882ValThr: 2.882 ± 0.033	3.73ValVal: 3.73 ± 0.035	0.733ValTrp: 0.733 ± 0.015	1.366ValTyr: 1.366 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	0.938TrpAla: 0.938 ± 0.014	0.162TrpCys: 0.162 ± 0.007	0.71TrpAsp: 0.71 ± 0.013	0.637TrpGlu: 0.637 ± 0.012	0.423TrpPhe: 0.423 ± 0.012	0.676TrpGly: 0.676 ± 0.015	0.332TrpHis: 0.332 ± 0.01	0.643TrpIle: 0.643 ± 0.014	0.681TrpLys: 0.681 ± 0.013	1.156TrpLeu: 1.156 ± 0.019	0.284TrpMet: 0.284 ± 0.008	0.527TrpAsn: 0.527 ± 0.012	0.542TrpPro: 0.542 ± 0.012	0.567TrpGln: 0.567 ± 0.013	0.728TrpArg: 0.728 ± 0.014	1.059TrpSer: 1.059 ± 0.017	0.738TrpThr: 0.738 ± 0.012	0.641TrpVal: 0.641 ± 0.014	0.218TrpTrp: 0.218 ± 0.008	0.304TrpTyr: 0.304 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.983TyrAla: 1.983 ± 0.024	0.313TyrCys: 0.313 ± 0.009	1.425TyrAsp: 1.425 ± 0.022	1.188TyrGlu: 1.188 ± 0.019	0.84TyrPhe: 0.84 ± 0.016	1.706TyrGly: 1.706 ± 0.031	0.651TyrHis: 0.651 ± 0.014	1.031TyrIle: 1.031 ± 0.019	0.928TyrLys: 0.928 ± 0.015	2.258TyrLeu: 2.258 ± 0.023	0.432TyrMet: 0.432 ± 0.009	0.878TyrAsn: 0.878 ± 0.018	1.222TyrPro: 1.222 ± 0.021	0.937TyrGln: 0.937 ± 0.017	1.419TyrArg: 1.419 ± 0.023	1.818TyrSer: 1.818 ± 0.024	1.308TyrThr: 1.308 ± 0.019	1.296TyrVal: 1.296 ± 0.017	0.288TyrTrp: 0.288 ± 0.01	0.646TyrTyr: 0.646 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 7111 proteins (4035239 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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