Candidatus Uzinura diaspidicola str. ASNER

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriales incertae sedis; Candidatus Uzinura; Candidatus Uzinura diaspidicola

Average proteome isoelectric point is 8.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7VKH8|L7VKH8_9FLAO Alanine--tRNA ligase OS=Candidatus Uzinura diaspidicola str. ASNER OX=1133592 GN=alaS PE=3 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.21LASHH10 pKa = 7.01LEE12 pKa = 4.12DD13 pKa = 5.37APWPANKK20 pKa = 10.13EE21 pKa = 3.94EE22 pKa = 5.04LIDD25 pKa = 3.62YY26 pKa = 8.75AIRR29 pKa = 11.84SGAPLEE35 pKa = 4.52IIEE38 pKa = 4.44NLQEE42 pKa = 4.62LEE44 pKa = 4.17DD45 pKa = 4.27DD46 pKa = 4.11SEE48 pKa = 4.48IFEE51 pKa = 4.61SIEE54 pKa = 4.95DD55 pKa = 3.23IWIDD59 pKa = 3.7YY60 pKa = 7.49PTQEE64 pKa = 4.85DD65 pKa = 4.83FFFNEE70 pKa = 4.49DD71 pKa = 3.13EE72 pKa = 4.46YY73 pKa = 11.99

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7VN29|L7VN29_9FLAO Succinate--CoA ligase [ADP-forming] subunit beta OS=Candidatus Uzinura diaspidicola str. ASNER OX=1133592 GN=sucC PE=3 SV=1
MM1 pKa = 7.54PHH3 pKa = 6.67PKK5 pKa = 9.5RR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.51SKK10 pKa = 10.15AKK12 pKa = 8.11RR13 pKa = 11.84DD14 pKa = 3.45KK15 pKa = 11.02RR16 pKa = 11.84RR17 pKa = 11.84THH19 pKa = 6.35HH20 pKa = 7.14KK21 pKa = 10.55AILPQLTSCPLTGQIHH37 pKa = 7.11LYY39 pKa = 9.45HH40 pKa = 6.67RR41 pKa = 11.84AYY43 pKa = 9.44WHH45 pKa = 7.39DD46 pKa = 3.76DD47 pKa = 2.71KK48 pKa = 11.53LFYY51 pKa = 10.12RR52 pKa = 11.84GRR54 pKa = 11.84VILQKK59 pKa = 10.78ADD61 pKa = 3.45YY62 pKa = 10.48

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

227

0

227

75735

38

1429

333.6

38.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.76 ± 0.117

1.12 ± 0.037

4.53 ± 0.063

6.553 ± 0.112

4.917 ± 0.105

6.092 ± 0.148

2.198 ± 0.065

11.012 ± 0.151

9.708 ± 0.159

9.228 ± 0.112

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.055 ± 0.059

5.598 ± 0.095

3.094 ± 0.069

2.919 ± 0.065

4.443 ± 0.121

7.164 ± 0.105

4.468 ± 0.071

5.081 ± 0.102

0.737 ± 0.057

4.322 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski