Arcticibacter svalbardensis MN12-7
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4309 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9GMY0|R9GMY0_9SPHI RNA polymerase ECF-type sigma factor OS=Arcticibacter svalbardensis MN12-7 OX=1150600 GN=ADIARSV_3755 PE=3 SV=1
MM1 pKa = 7.46 LKK3 pKa = 9.5 MVEE6 pKa = 4.22 TEE8 pKa = 4.09 QALMTYY14 pKa = 9.96 YY15 pKa = 11.18 GLPFTLKK22 pKa = 10.47 YY23 pKa = 10.68 SEE25 pKa = 4.78 LFQFFALKK33 pKa = 10.34 EE34 pKa = 4.13 DD35 pKa = 4.03 FVLADD40 pKa = 3.05 IAEE43 pKa = 5.11 FILSLEE49 pKa = 4.21 EE50 pKa = 3.87 AANEE54 pKa = 4.32 YY55 pKa = 10.22 ILQPVLTDD63 pKa = 3.41 VQLLQQAKK71 pKa = 9.14 EE72 pKa = 4.06 NSLKK76 pKa = 11.06 AEE78 pKa = 4.18 DD79 pKa = 4.11 VLPEE83 pKa = 4.48 LSLHH87 pKa = 6.25 LQPQHH92 pKa = 6.66 YY93 pKa = 10.35 YY94 pKa = 10.82 LFLYY98 pKa = 10.43 DD99 pKa = 4.42 VLFLQEE105 pKa = 5.72 DD106 pKa = 3.35 IDD108 pKa = 3.95 AAAILQEE115 pKa = 4.0 MQLVSEE121 pKa = 4.18 NQIAGRR127 pKa = 11.84 LHH129 pKa = 7.38 LIGIDD134 pKa = 3.45 NMLLAEE140 pKa = 4.86 EE141 pKa = 5.03 DD142 pKa = 4.14 NNDD145 pKa = 2.89 IWEE148 pKa = 4.49 MGLQFLGDD156 pKa = 3.56 FMGGYY161 pKa = 9.01 PRR163 pKa = 11.84 YY164 pKa = 10.24 AEE166 pKa = 4.56 EE167 pKa = 3.94 NLCNKK172 pKa = 9.4 SANPFDD178 pKa = 5.24 PDD180 pKa = 4.31 YY181 pKa = 11.73 DD182 pKa = 5.8 DD183 pKa = 6.79 DD184 pKa = 7.37 DD185 pKa = 7.44 DD186 pKa = 6.15 DD187 pKa = 5.98 DD188 pKa = 6.03 KK189 pKa = 11.64 PDD191 pKa = 4.9 PFDD194 pKa = 5.21 PDD196 pKa = 3.77 YY197 pKa = 11.82 NDD199 pKa = 3.7 IKK201 pKa = 10.52 PDD203 pKa = 3.7 PFII206 pKa = 6.08
Molecular weight: 23.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.694
IPC_protein 3.694
Toseland 3.478
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.528
Grimsley 3.389
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.821
Patrickios 1.125
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|R9GXV9|R9GXV9_9SPHI Uncharacterized protein OS=Arcticibacter svalbardensis MN12-7 OX=1150600 GN=ADIARSV_3047 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 10.5 LFNRR6 pKa = 11.84 TQQTKK11 pKa = 9.83 YY12 pKa = 10.47 AIEE15 pKa = 4.13 HH16 pKa = 5.97 EE17 pKa = 4.36 RR18 pKa = 11.84 LAGTIASFIIRR29 pKa = 11.84 IQQRR33 pKa = 11.84 IASFLNEE40 pKa = 4.77 GILRR44 pKa = 11.84 IPPSLRR50 pKa = 11.84 LILLLATGLAFAACCLYY67 pKa = 11.26 LLIKK71 pKa = 10.13 GFKK74 pKa = 9.78
Molecular weight: 8.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.619
IPC_protein 10.101
Toseland 10.482
ProMoST 10.233
Dawson 10.613
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.965
Grimsley 10.657
Solomon 10.687
Lehninger 10.657
Nozaki 10.496
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.54
Patrickios 10.774
IPC_peptide 10.687
IPC2_peptide 9.399
IPC2.peptide.svr19 8.485
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4309
0
4309
1348798
37
5671
313.0
35.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.911 ± 0.036
0.783 ± 0.011
5.361 ± 0.03
5.807 ± 0.04
4.947 ± 0.033
6.652 ± 0.042
1.827 ± 0.018
7.747 ± 0.032
7.137 ± 0.043
9.692 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.325 ± 0.021
5.661 ± 0.04
3.717 ± 0.017
3.598 ± 0.023
3.821 ± 0.025
6.861 ± 0.035
5.666 ± 0.055
6.253 ± 0.034
1.114 ± 0.016
4.118 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here