Changjiang tombus-like virus 18

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG06|A0A1L3KG06_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 18 OX=1922811 PE=4 SV=1
MM1 pKa = 7.66FKK3 pKa = 9.91TLNQKK8 pKa = 9.25QLALARR14 pKa = 11.84TRR16 pKa = 11.84SKK18 pKa = 10.85KK19 pKa = 10.02ILANRR24 pKa = 11.84YY25 pKa = 8.44LNCRR29 pKa = 11.84YY30 pKa = 10.37NPFEE34 pKa = 4.16SRR36 pKa = 11.84GGAMIPDD43 pKa = 5.35GIGKK47 pKa = 8.97HH48 pKa = 5.52LEE50 pKa = 4.0SRR52 pKa = 11.84DD53 pKa = 3.46FRR55 pKa = 11.84SIYY58 pKa = 9.04HH59 pKa = 6.33LKK61 pKa = 8.94VTGQAEE67 pKa = 4.48VLILPAIPMVAAVISPEE84 pKa = 4.01GNTLEE89 pKa = 4.19VNGMSIINGSYY100 pKa = 10.6VGLCGSNLLKK110 pKa = 11.13YY111 pKa = 7.17NTPLGGTVVQGDD123 pKa = 4.47DD124 pKa = 3.42VTSARR129 pKa = 11.84IVTVGYY135 pKa = 10.4RR136 pKa = 11.84LTYY139 pKa = 9.55EE140 pKa = 4.6GKK142 pKa = 8.27ATEE145 pKa = 4.4ANGQLVANSLPLMLDD160 pKa = 3.59GSPMLNSGSYY170 pKa = 9.93VFSGGNPVTKK180 pKa = 10.47VAGSMTAINIDD191 pKa = 3.81MEE193 pKa = 4.73TNAHH197 pKa = 4.68VTTRR201 pKa = 11.84DD202 pKa = 3.25TKK204 pKa = 10.32IFRR207 pKa = 11.84PEE209 pKa = 3.59EE210 pKa = 4.03GVAGVLKK217 pKa = 10.62RR218 pKa = 11.84QVTVANHH225 pKa = 5.72AFKK228 pKa = 10.6PFWEE232 pKa = 4.35TGVYY236 pKa = 9.84PNNLNGLDD244 pKa = 3.86NVSKK248 pKa = 10.65AAWLQNPPAGLVDD261 pKa = 4.05NNSISYY267 pKa = 7.53PTVAVLDD274 pKa = 3.81HH275 pKa = 6.72ALGVEE280 pKa = 4.41SIVINNDD287 pKa = 2.23NTTPLLYY294 pKa = 10.19RR295 pKa = 11.84LEE297 pKa = 5.16IIHH300 pKa = 6.57CVQFQHH306 pKa = 6.36SPNWGLADD314 pKa = 3.79LTVDD318 pKa = 3.98APAVDD323 pKa = 3.99KK324 pKa = 11.12QALEE328 pKa = 4.02ADD330 pKa = 3.84DD331 pKa = 4.8RR332 pKa = 11.84LNSTVTHH339 pKa = 6.32AVDD342 pKa = 3.7ATSSTIPTRR351 pKa = 11.84YY352 pKa = 9.8SSTVGPATSSIFSDD366 pKa = 3.27VTMDD370 pKa = 3.48MSTTKK375 pKa = 8.27TTTRR379 pKa = 11.84SPYY382 pKa = 10.98ARR384 pKa = 11.84TTTVLQQPKK393 pKa = 10.18RR394 pKa = 11.84KK395 pKa = 8.32ATTKK399 pKa = 9.21ATPSPAKK406 pKa = 10.3KK407 pKa = 10.06AGKK410 pKa = 9.56KK411 pKa = 9.55

Molecular weight: 44.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFU2|A0A1L3KFU2_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 18 OX=1922811 PE=4 SV=1
MM1 pKa = 7.07MLSSSVNSPVTPALSAQTPQRR22 pKa = 11.84IGGIQSKK29 pKa = 7.97RR30 pKa = 11.84QKK32 pKa = 10.4VMGYY36 pKa = 9.68LRR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 8.06LAKK43 pKa = 9.94VPYY46 pKa = 8.86HH47 pKa = 6.36VNPALTVRR55 pKa = 11.84DD56 pKa = 3.73LHH58 pKa = 8.02VDD60 pKa = 3.49EE61 pKa = 5.54PLSKK65 pKa = 10.24EE66 pKa = 3.44QTKK69 pKa = 10.09RR70 pKa = 11.84VEE72 pKa = 3.91EE73 pKa = 4.47FIEE76 pKa = 4.3VVEE79 pKa = 4.85DD80 pKa = 3.96PKK82 pKa = 11.27SSQALKK88 pKa = 10.41DD89 pKa = 3.26NRR91 pKa = 11.84YY92 pKa = 10.25AILEE96 pKa = 4.38DD97 pKa = 4.09EE98 pKa = 4.72VKK100 pKa = 9.33HH101 pKa = 6.18TIIQMPSPTGNTYY114 pKa = 9.45QVIRR118 pKa = 11.84NKK120 pKa = 8.82TPKK123 pKa = 9.27VKK125 pKa = 10.08KK126 pKa = 10.48VKK128 pKa = 9.7IDD130 pKa = 3.6SPVMPEE136 pKa = 3.65EE137 pKa = 4.18MFPDD141 pKa = 3.12RR142 pKa = 11.84QVINRR147 pKa = 11.84QVKK150 pKa = 10.2KK151 pKa = 10.75DD152 pKa = 3.68GILAKK157 pKa = 9.74TNPSLVYY164 pKa = 9.37YY165 pKa = 10.68LRR167 pKa = 11.84TKK169 pKa = 10.68FFLKK173 pKa = 10.56FRR175 pKa = 11.84DD176 pKa = 3.6HH177 pKa = 7.85ALMQTMVHH185 pKa = 6.03EE186 pKa = 4.35ARR188 pKa = 11.84MWLLKK193 pKa = 10.67NGHH196 pKa = 5.47TCDD199 pKa = 3.44NKK201 pKa = 10.17VDD203 pKa = 3.89YY204 pKa = 7.34EE205 pKa = 4.45TLTSAVTVAFMITKK219 pKa = 10.1EE220 pKa = 4.1EE221 pKa = 3.83IAFRR225 pKa = 11.84QLIKK229 pKa = 10.76NKK231 pKa = 10.34KK232 pKa = 8.36NFDD235 pKa = 3.73NMVHH239 pKa = 6.45LNKK242 pKa = 9.93TVTGNLGRR250 pKa = 11.84AAGLRR255 pKa = 11.84EE256 pKa = 4.42VKK258 pKa = 10.66SLGHH262 pKa = 5.86SFLSEE267 pKa = 3.63VHH269 pKa = 6.43LPTRR273 pKa = 11.84PIVAA277 pKa = 4.2

Molecular weight: 31.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1113

277

425

371.0

41.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.469 ± 1.065

1.078 ± 0.378

4.762 ± 0.199

4.762 ± 0.625

3.953 ± 1.054

5.391 ± 0.899

2.156 ± 0.36

5.93 ± 0.751

7.008 ± 0.97

8.715 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.875 ± 0.356

5.571 ± 0.581

4.762 ± 0.796

3.774 ± 0.393

6.11 ± 0.891

7.996 ± 0.936

7.457 ± 1.219

6.739 ± 1.719

0.898 ± 0.247

3.594 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski