Mycobacterium phage DillTech15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1PB72|A0A2S1PB72_9CAUD Uncharacterized protein OS=Mycobacterium phage DillTech15 OX=2163591 GN=104 PE=4 SV=1
MM1 pKa = 7.98PDD3 pKa = 3.43SDD5 pKa = 5.65PIDD8 pKa = 3.45FTAAGEE14 pKa = 4.09AVAEE18 pKa = 4.15IFMDD22 pKa = 4.95GIRR25 pKa = 11.84AIIAQEE31 pKa = 3.5LDD33 pKa = 3.05ARR35 pKa = 11.84GVKK38 pKa = 10.45GPSTVVNNVVPYY50 pKa = 10.34SLPDD54 pKa = 3.59SQDD57 pKa = 3.03AQYY60 pKa = 10.67MEE62 pKa = 4.28ATYY65 pKa = 10.41EE66 pKa = 4.01ADD68 pKa = 3.24VV69 pKa = 3.7

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1PB35|A0A2S1PB35_9CAUD 4Fe-4S Wbl-type domain-containing protein OS=Mycobacterium phage DillTech15 OX=2163591 GN=56 PE=3 SV=1
MM1 pKa = 7.49NNNTKK6 pKa = 10.34RR7 pKa = 11.84AGRR10 pKa = 11.84PEE12 pKa = 3.61VGRR15 pKa = 11.84PVNVRR20 pKa = 11.84LGDD23 pKa = 3.99DD24 pKa = 3.89LLAEE28 pKa = 4.15VDD30 pKa = 3.97EE31 pKa = 4.56YY32 pKa = 11.81AAAEE36 pKa = 4.85GIARR40 pKa = 11.84AEE42 pKa = 4.62AIRR45 pKa = 11.84HH46 pKa = 5.31LLRR49 pKa = 11.84RR50 pKa = 11.84GLKK53 pKa = 9.27RR54 pKa = 11.84GKK56 pKa = 10.02RR57 pKa = 3.59

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

111

0

111

18381

25

1176

165.6

18.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.429 ± 0.376

1.268 ± 0.167

6.632 ± 0.225

5.718 ± 0.354

2.921 ± 0.216

8.819 ± 0.613

2.263 ± 0.213

4.325 ± 0.204

3.389 ± 0.227

7.247 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.143

3.351 ± 0.164

6.142 ± 0.192

3.438 ± 0.185

6.985 ± 0.405

5.974 ± 0.308

6.692 ± 0.203

7.214 ± 0.298

2.41 ± 0.158

2.508 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski