Amino acid dipepetide frequency for Azospirillum phage Cd

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.559AlaAla: 21.559 ± 2.208
1.281AlaCys: 1.281 ± 0.233
7.91AlaAsp: 7.91 ± 0.679
9.526AlaGlu: 9.526 ± 0.998
3.732AlaPhe: 3.732 ± 0.513
10.974AlaGly: 10.974 ± 0.93
1.894AlaHis: 1.894 ± 0.321
5.292AlaIle: 5.292 ± 0.673
6.852AlaLys: 6.852 ± 0.736
13.091AlaLeu: 13.091 ± 0.933
3.231AlaMet: 3.231 ± 0.473
3.454AlaAsn: 3.454 ± 0.587
4.178AlaPro: 4.178 ± 0.517
4.234AlaGln: 4.234 ± 0.524
9.192AlaArg: 9.192 ± 0.882
7.799AlaSer: 7.799 ± 0.776
8.746AlaThr: 8.746 ± 0.81
8.802AlaVal: 8.802 ± 0.714
2.451AlaTrp: 2.451 ± 0.383
2.563AlaTyr: 2.563 ± 0.395
0.0AlaXaa: 0.0 ± 0.0
Cys
1.337CysAla: 1.337 ± 0.345
0.111CysCys: 0.111 ± 0.084
1.226CysAsp: 1.226 ± 0.26
0.501CysGlu: 0.501 ± 0.169
0.39CysPhe: 0.39 ± 0.183
1.17CysGly: 1.17 ± 0.333
0.279CysHis: 0.279 ± 0.136
0.557CysIle: 0.557 ± 0.171
0.501CysLys: 0.501 ± 0.209
1.003CysLeu: 1.003 ± 0.274
0.167CysMet: 0.167 ± 0.096
0.446CysAsn: 0.446 ± 0.162
0.557CysPro: 0.557 ± 0.205
0.501CysGln: 0.501 ± 0.186
1.393CysArg: 1.393 ± 0.34
0.613CysSer: 0.613 ± 0.176
0.78CysThr: 0.78 ± 0.254
0.334CysVal: 0.334 ± 0.127
0.223CysTrp: 0.223 ± 0.11
0.056CysTyr: 0.056 ± 0.045
0.0CysXaa: 0.0 ± 0.0
Asp
8.133AspAla: 8.133 ± 0.689
0.557AspCys: 0.557 ± 0.24
3.565AspAsp: 3.565 ± 0.432
4.457AspGlu: 4.457 ± 0.683
1.504AspPhe: 1.504 ± 0.27
6.908AspGly: 6.908 ± 0.72
1.058AspHis: 1.058 ± 0.325
2.73AspIle: 2.73 ± 0.315
3.008AspLys: 3.008 ± 0.413
4.679AspLeu: 4.679 ± 0.529
1.616AspMet: 1.616 ± 0.311
1.783AspAsn: 1.783 ± 0.315
3.454AspPro: 3.454 ± 0.444
1.671AspGln: 1.671 ± 0.297
4.122AspArg: 4.122 ± 0.447
2.173AspSer: 2.173 ± 0.409
2.785AspThr: 2.785 ± 0.546
2.841AspVal: 2.841 ± 0.313
1.058AspTrp: 1.058 ± 0.298
1.281AspTyr: 1.281 ± 0.259
0.0AspXaa: 0.0 ± 0.0
Glu
10.027GluAla: 10.027 ± 0.904
0.836GluCys: 0.836 ± 0.229
2.73GluAsp: 2.73 ± 0.349
3.398GluGlu: 3.398 ± 0.531
1.838GluPhe: 1.838 ± 0.338
2.674GluGly: 2.674 ± 0.389
0.947GluHis: 0.947 ± 0.273
2.897GluIle: 2.897 ± 0.433
3.008GluLys: 3.008 ± 0.486
4.958GluLeu: 4.958 ± 0.587
2.173GluMet: 2.173 ± 0.357
1.226GluAsn: 1.226 ± 0.272
2.841GluPro: 2.841 ± 0.465
2.451GluGln: 2.451 ± 0.461
4.234GluArg: 4.234 ± 0.421
2.173GluSer: 2.173 ± 0.336
3.454GluThr: 3.454 ± 0.567
2.618GluVal: 2.618 ± 0.346
1.003GluTrp: 1.003 ± 0.268
1.114GluTyr: 1.114 ± 0.244
0.0GluXaa: 0.0 ± 0.0
Phe
3.9PheAla: 3.9 ± 0.384
0.446PheCys: 0.446 ± 0.162
1.95PheAsp: 1.95 ± 0.327
1.17PheGlu: 1.17 ± 0.287
0.836PhePhe: 0.836 ± 0.22
2.173PheGly: 2.173 ± 0.375
0.39PheHis: 0.39 ± 0.167
1.003PheIle: 1.003 ± 0.242
0.836PheLys: 0.836 ± 0.258
1.56PheLeu: 1.56 ± 0.27
0.613PheMet: 0.613 ± 0.2
1.17PheAsn: 1.17 ± 0.249
2.173PhePro: 2.173 ± 0.443
1.003PheGln: 1.003 ± 0.237
2.061PheArg: 2.061 ± 0.382
1.504PheSer: 1.504 ± 0.271
1.616PheThr: 1.616 ± 0.314
2.117PheVal: 2.117 ± 0.36
0.279PheTrp: 0.279 ± 0.133
0.613PheTyr: 0.613 ± 0.174
0.0PheXaa: 0.0 ± 0.0
Gly
8.189GlyAla: 8.189 ± 0.768
1.114GlyCys: 1.114 ± 0.364
4.735GlyAsp: 4.735 ± 0.548
3.565GlyGlu: 3.565 ± 0.507
3.008GlyPhe: 3.008 ± 0.354
7.465GlyGly: 7.465 ± 0.729
1.95GlyHis: 1.95 ± 0.334
3.844GlyIle: 3.844 ± 0.43
3.788GlyLys: 3.788 ± 0.481
7.91GlyLeu: 7.91 ± 0.673
3.51GlyMet: 3.51 ± 0.444
2.451GlyAsn: 2.451 ± 0.427
2.841GlyPro: 2.841 ± 0.47
3.565GlyGln: 3.565 ± 0.49
4.791GlyArg: 4.791 ± 0.725
4.679GlySer: 4.679 ± 0.883
6.295GlyThr: 6.295 ± 1.022
6.518GlyVal: 6.518 ± 0.57
2.34GlyTrp: 2.34 ± 0.303
2.451GlyTyr: 2.451 ± 0.506
0.0GlyXaa: 0.0 ± 0.0
His
2.34HisAla: 2.34 ± 0.469
0.223HisCys: 0.223 ± 0.125
1.058HisAsp: 1.058 ± 0.241
0.78HisGlu: 0.78 ± 0.186
0.39HisPhe: 0.39 ± 0.135
1.56HisGly: 1.56 ± 0.355
0.891HisHis: 0.891 ± 0.321
0.613HisIle: 0.613 ± 0.224
0.557HisLys: 0.557 ± 0.209
2.34HisLeu: 2.34 ± 0.372
0.446HisMet: 0.446 ± 0.146
0.724HisAsn: 0.724 ± 0.215
1.337HisPro: 1.337 ± 0.325
0.279HisGln: 0.279 ± 0.151
1.783HisArg: 1.783 ± 0.301
1.281HisSer: 1.281 ± 0.272
0.613HisThr: 0.613 ± 0.187
1.003HisVal: 1.003 ± 0.267
0.39HisTrp: 0.39 ± 0.14
0.39HisTyr: 0.39 ± 0.153
0.0HisXaa: 0.0 ± 0.0
Ile
6.573IleAla: 6.573 ± 0.629
0.724IleCys: 0.724 ± 0.202
2.395IleAsp: 2.395 ± 0.318
2.785IleGlu: 2.785 ± 0.375
0.39IlePhe: 0.39 ± 0.146
5.069IleGly: 5.069 ± 0.577
1.114IleHis: 1.114 ± 0.251
1.393IleIle: 1.393 ± 0.32
1.058IleLys: 1.058 ± 0.246
1.838IleLeu: 1.838 ± 0.378
0.501IleMet: 0.501 ± 0.216
1.281IleAsn: 1.281 ± 0.332
1.783IlePro: 1.783 ± 0.306
1.226IleGln: 1.226 ± 0.267
2.897IleArg: 2.897 ± 0.358
2.005IleSer: 2.005 ± 0.301
3.342IleThr: 3.342 ± 0.443
2.34IleVal: 2.34 ± 0.319
0.613IleTrp: 0.613 ± 0.165
0.557IleTyr: 0.557 ± 0.182
0.0IleXaa: 0.0 ± 0.0
Lys
7.465LysAla: 7.465 ± 0.887
0.446LysCys: 0.446 ± 0.196
2.73LysAsp: 2.73 ± 0.411
2.34LysGlu: 2.34 ± 0.363
0.724LysPhe: 0.724 ± 0.192
2.73LysGly: 2.73 ± 0.49
0.891LysHis: 0.891 ± 0.258
1.17LysIle: 1.17 ± 0.284
1.727LysLys: 1.727 ± 0.308
3.342LysLeu: 3.342 ± 0.405
0.891LysMet: 0.891 ± 0.216
0.78LysAsn: 0.78 ± 0.221
2.73LysPro: 2.73 ± 0.524
0.947LysGln: 0.947 ± 0.239
4.624LysArg: 4.624 ± 0.522
2.897LysSer: 2.897 ± 0.489
2.061LysThr: 2.061 ± 0.359
2.674LysVal: 2.674 ± 0.412
0.557LysTrp: 0.557 ± 0.186
0.557LysTyr: 0.557 ± 0.228
0.0LysXaa: 0.0 ± 0.0
Leu
10.027LeuAla: 10.027 ± 0.814
1.504LeuCys: 1.504 ± 0.379
5.404LeuAsp: 5.404 ± 0.547
4.958LeuGlu: 4.958 ± 0.588
1.894LeuPhe: 1.894 ± 0.267
7.075LeuGly: 7.075 ± 0.979
1.448LeuHis: 1.448 ± 0.381
2.952LeuIle: 2.952 ± 0.438
3.175LeuLys: 3.175 ± 0.451
6.128LeuLeu: 6.128 ± 0.663
1.671LeuMet: 1.671 ± 0.33
2.395LeuAsn: 2.395 ± 0.378
4.457LeuPro: 4.457 ± 0.487
1.838LeuGln: 1.838 ± 0.286
6.685LeuArg: 6.685 ± 0.53
6.406LeuSer: 6.406 ± 0.703
5.348LeuThr: 5.348 ± 0.523
5.404LeuVal: 5.404 ± 0.653
1.448LeuTrp: 1.448 ± 0.357
1.894LeuTyr: 1.894 ± 0.296
0.0LeuXaa: 0.0 ± 0.0
Met
3.175MetAla: 3.175 ± 0.398
0.167MetCys: 0.167 ± 0.112
1.281MetAsp: 1.281 ± 0.242
1.56MetGlu: 1.56 ± 0.264
0.446MetPhe: 0.446 ± 0.169
1.783MetGly: 1.783 ± 0.425
0.223MetHis: 0.223 ± 0.119
1.058MetIle: 1.058 ± 0.226
1.058MetLys: 1.058 ± 0.232
2.284MetLeu: 2.284 ± 0.381
0.446MetMet: 0.446 ± 0.173
0.78MetAsn: 0.78 ± 0.179
1.616MetPro: 1.616 ± 0.374
1.058MetGln: 1.058 ± 0.238
2.785MetArg: 2.785 ± 0.329
2.117MetSer: 2.117 ± 0.359
2.952MetThr: 2.952 ± 0.519
1.671MetVal: 1.671 ± 0.329
0.334MetTrp: 0.334 ± 0.135
0.279MetTyr: 0.279 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
4.067AsnAla: 4.067 ± 0.603
0.223AsnCys: 0.223 ± 0.102
1.17AsnAsp: 1.17 ± 0.206
1.17AsnGlu: 1.17 ± 0.295
1.058AsnPhe: 1.058 ± 0.287
3.454AsnGly: 3.454 ± 0.435
0.223AsnHis: 0.223 ± 0.087
0.891AsnIle: 0.891 ± 0.236
0.613AsnLys: 0.613 ± 0.264
2.34AsnLeu: 2.34 ± 0.374
0.891AsnMet: 0.891 ± 0.199
1.114AsnAsn: 1.114 ± 0.288
1.838AsnPro: 1.838 ± 0.277
1.003AsnGln: 1.003 ± 0.225
2.117AsnArg: 2.117 ± 0.324
1.783AsnSer: 1.783 ± 0.301
1.56AsnThr: 1.56 ± 0.393
2.005AsnVal: 2.005 ± 0.405
0.557AsnTrp: 0.557 ± 0.159
0.446AsnTyr: 0.446 ± 0.14
0.0AsnXaa: 0.0 ± 0.0
Pro
6.128ProAla: 6.128 ± 0.724
0.836ProCys: 0.836 ± 0.265
3.454ProAsp: 3.454 ± 0.511
2.73ProGlu: 2.73 ± 0.406
1.616ProPhe: 1.616 ± 0.355
4.401ProGly: 4.401 ± 0.48
1.058ProHis: 1.058 ± 0.287
1.838ProIle: 1.838 ± 0.281
1.504ProLys: 1.504 ± 0.29
3.677ProLeu: 3.677 ± 0.553
1.838ProMet: 1.838 ± 0.361
1.448ProAsn: 1.448 ± 0.336
2.618ProPro: 2.618 ± 0.533
1.281ProGln: 1.281 ± 0.342
2.507ProArg: 2.507 ± 0.439
3.231ProSer: 3.231 ± 0.5
3.287ProThr: 3.287 ± 0.488
3.955ProVal: 3.955 ± 0.479
1.616ProTrp: 1.616 ± 0.305
0.78ProTyr: 0.78 ± 0.174
0.0ProXaa: 0.0 ± 0.0
Gln
4.679GlnAla: 4.679 ± 0.524
0.223GlnCys: 0.223 ± 0.118
1.226GlnAsp: 1.226 ± 0.284
1.95GlnGlu: 1.95 ± 0.378
0.947GlnPhe: 0.947 ± 0.208
2.451GlnGly: 2.451 ± 0.351
0.446GlnHis: 0.446 ± 0.149
1.838GlnIle: 1.838 ± 0.411
1.838GlnLys: 1.838 ± 0.392
2.34GlnLeu: 2.34 ± 0.309
0.947GlnMet: 0.947 ± 0.232
0.836GlnAsn: 0.836 ± 0.204
2.228GlnPro: 2.228 ± 0.403
1.504GlnGln: 1.504 ± 0.39
2.952GlnArg: 2.952 ± 0.413
1.783GlnSer: 1.783 ± 0.354
1.616GlnThr: 1.616 ± 0.283
2.34GlnVal: 2.34 ± 0.363
0.39GlnTrp: 0.39 ± 0.13
0.724GlnTyr: 0.724 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
8.69ArgAla: 8.69 ± 0.776
1.114ArgCys: 1.114 ± 0.321
4.679ArgAsp: 4.679 ± 0.53
3.955ArgGlu: 3.955 ± 0.487
2.618ArgPhe: 2.618 ± 0.377
4.234ArgGly: 4.234 ± 0.436
2.563ArgHis: 2.563 ± 0.496
2.841ArgIle: 2.841 ± 0.462
3.287ArgLys: 3.287 ± 0.392
6.908ArgLeu: 6.908 ± 0.725
2.785ArgMet: 2.785 ± 0.476
1.894ArgAsn: 1.894 ± 0.306
3.064ArgPro: 3.064 ± 0.52
2.897ArgGln: 2.897 ± 0.425
6.963ArgArg: 6.963 ± 0.912
4.289ArgSer: 4.289 ± 0.61
3.287ArgThr: 3.287 ± 0.393
4.902ArgVal: 4.902 ± 0.54
1.783ArgTrp: 1.783 ± 0.357
1.671ArgTyr: 1.671 ± 0.289
0.0ArgXaa: 0.0 ± 0.0
Ser
8.746SerAla: 8.746 ± 0.683
0.501SerCys: 0.501 ± 0.167
3.287SerAsp: 3.287 ± 0.406
2.005SerGlu: 2.005 ± 0.385
1.56SerPhe: 1.56 ± 0.281
6.351SerGly: 6.351 ± 0.774
0.78SerHis: 0.78 ± 0.223
2.173SerIle: 2.173 ± 0.459
2.34SerLys: 2.34 ± 0.429
5.682SerLeu: 5.682 ± 0.573
1.56SerMet: 1.56 ± 0.291
1.894SerAsn: 1.894 ± 0.36
3.231SerPro: 3.231 ± 0.555
1.783SerGln: 1.783 ± 0.298
3.175SerArg: 3.175 ± 0.419
4.067SerSer: 4.067 ± 0.652
3.398SerThr: 3.398 ± 0.664
3.9SerVal: 3.9 ± 0.44
0.836SerTrp: 0.836 ± 0.191
1.616SerTyr: 1.616 ± 0.365
0.0SerXaa: 0.0 ± 0.0
Thr
9.303ThrAla: 9.303 ± 1.185
0.446ThrCys: 0.446 ± 0.184
4.122ThrAsp: 4.122 ± 0.493
3.788ThrGlu: 3.788 ± 0.376
1.671ThrPhe: 1.671 ± 0.365
5.571ThrGly: 5.571 ± 0.846
0.947ThrHis: 0.947 ± 0.263
2.618ThrIle: 2.618 ± 0.469
3.175ThrLys: 3.175 ± 0.383
4.624ThrLeu: 4.624 ± 0.788
1.058ThrMet: 1.058 ± 0.226
1.56ThrAsn: 1.56 ± 0.406
3.788ThrPro: 3.788 ± 0.484
1.616ThrGln: 1.616 ± 0.276
3.51ThrArg: 3.51 ± 0.385
2.952ThrSer: 2.952 ± 0.534
4.011ThrThr: 4.011 ± 0.641
4.847ThrVal: 4.847 ± 0.612
0.891ThrTrp: 0.891 ± 0.227
1.894ThrTyr: 1.894 ± 0.407
0.0ThrXaa: 0.0 ± 0.0
Val
8.022ValAla: 8.022 ± 0.89
0.947ValCys: 0.947 ± 0.289
4.067ValAsp: 4.067 ± 0.365
3.342ValGlu: 3.342 ± 0.491
1.95ValPhe: 1.95 ± 0.323
5.849ValGly: 5.849 ± 0.575
1.226ValHis: 1.226 ± 0.313
2.618ValIle: 2.618 ± 0.372
2.785ValLys: 2.785 ± 0.464
4.345ValLeu: 4.345 ± 0.446
1.727ValMet: 1.727 ± 0.316
2.005ValAsn: 2.005 ± 0.305
3.51ValPro: 3.51 ± 0.527
2.117ValGln: 2.117 ± 0.394
4.457ValArg: 4.457 ± 0.442
4.568ValSer: 4.568 ± 0.697
4.624ValThr: 4.624 ± 0.549
4.847ValVal: 4.847 ± 0.52
1.114ValTrp: 1.114 ± 0.257
1.393ValTyr: 1.393 ± 0.271
0.0ValXaa: 0.0 ± 0.0
Trp
1.448TrpAla: 1.448 ± 0.325
0.167TrpCys: 0.167 ± 0.098
0.891TrpAsp: 0.891 ± 0.225
1.114TrpGlu: 1.114 ± 0.22
0.279TrpPhe: 0.279 ± 0.125
1.504TrpGly: 1.504 ± 0.384
0.39TrpHis: 0.39 ± 0.148
0.836TrpIle: 0.836 ± 0.213
0.78TrpLys: 0.78 ± 0.265
1.56TrpLeu: 1.56 ± 0.272
0.557TrpMet: 0.557 ± 0.192
0.613TrpAsn: 0.613 ± 0.16
0.78TrpPro: 0.78 ± 0.192
1.003TrpGln: 1.003 ± 0.217
2.173TrpArg: 2.173 ± 0.314
1.17TrpSer: 1.17 ± 0.287
1.448TrpThr: 1.448 ± 0.212
1.337TrpVal: 1.337 ± 0.26
0.39TrpTrp: 0.39 ± 0.16
0.167TrpTyr: 0.167 ± 0.092
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.952TyrAla: 2.952 ± 0.369
0.279TyrCys: 0.279 ± 0.122
1.504TyrAsp: 1.504 ± 0.297
1.504TyrGlu: 1.504 ± 0.285
0.557TyrPhe: 0.557 ± 0.173
1.616TyrGly: 1.616 ± 0.32
0.279TyrHis: 0.279 ± 0.13
0.724TyrIle: 0.724 ± 0.276
0.39TyrLys: 0.39 ± 0.141
1.504TyrLeu: 1.504 ± 0.328
0.39TyrMet: 0.39 ± 0.156
0.668TyrAsn: 0.668 ± 0.221
0.78TyrPro: 0.78 ± 0.208
1.281TyrGln: 1.281 ± 0.271
2.005TyrArg: 2.005 ± 0.406
1.393TyrSer: 1.393 ± 0.29
1.17TyrThr: 1.17 ± 0.223
1.058TyrVal: 1.058 ± 0.224
0.334TyrTrp: 0.334 ± 0.117
0.557TyrTyr: 0.557 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (17952 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski