candidate division MSBL1 archaeon SCGC-AAA382N08

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VNQ8|A0A133VNQ8_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382N08 OX=1698285 GN=AKJ56_01915 PE=4 SV=1
MM1 pKa = 7.42NLLKK5 pKa = 10.38KK6 pKa = 10.61LSVIGLVAVFSFAMVGMALADD27 pKa = 3.67SASDD31 pKa = 3.61YY32 pKa = 10.81TSQADD37 pKa = 3.77CSDD40 pKa = 3.31AGYY43 pKa = 10.79YY44 pKa = 9.4WYY46 pKa = 9.37TGNEE50 pKa = 3.78TCYY53 pKa = 9.15EE54 pKa = 4.12TEE56 pKa = 3.93NAKK59 pKa = 10.73LSAQVSNLLEE69 pKa = 5.02RR70 pKa = 11.84IDD72 pKa = 4.51SLTATIAEE80 pKa = 4.26LTGDD84 pKa = 4.35EE85 pKa = 4.55EE86 pKa = 4.74PEE88 pKa = 4.06PATGGDD94 pKa = 3.47VPAVCEE100 pKa = 3.99GVTFDD105 pKa = 3.95RR106 pKa = 11.84ALDD109 pKa = 3.59VGMSGDD115 pKa = 5.68DD116 pKa = 3.76VLCLQTMLNQDD127 pKa = 3.81LSEE130 pKa = 5.23PIADD134 pKa = 4.48SGPGSPGNEE143 pKa = 3.22TSYY146 pKa = 10.81FGSITKK152 pKa = 10.47GGVVTFQEE160 pKa = 4.61NYY162 pKa = 10.32SEE164 pKa = 5.44DD165 pKa = 3.95VLAPWDD171 pKa = 3.78LTQGTGYY178 pKa = 10.34VGKK181 pKa = 7.18TTRR184 pKa = 11.84EE185 pKa = 4.05KK186 pKa = 11.16LNSLLDD192 pKa = 3.22QWAAEE197 pKa = 4.19EE198 pKa = 4.53KK199 pKa = 11.03APGDD203 pKa = 3.61YY204 pKa = 10.34TEE206 pKa = 5.27QSACEE211 pKa = 3.64DD212 pKa = 3.33AGYY215 pKa = 10.62YY216 pKa = 9.36WYY218 pKa = 10.17DD219 pKa = 3.61EE220 pKa = 4.38ACHH223 pKa = 5.99EE224 pKa = 4.25EE225 pKa = 4.34EE226 pKa = 4.56EE227 pKa = 4.88GEE229 pKa = 4.32EE230 pKa = 4.15VTGEE234 pKa = 4.08GLQVALADD242 pKa = 4.86DD243 pKa = 4.32NPAAATIISGTDD255 pKa = 2.95GGNAQSLVPFLKK267 pKa = 10.81VKK269 pKa = 10.83LEE271 pKa = 4.29NGDD274 pKa = 3.52NSAVDD279 pKa = 3.63VTQLDD284 pKa = 4.65FSRR287 pKa = 11.84SGISSDD293 pKa = 3.25SDD295 pKa = 2.98ISQGYY300 pKa = 8.85LFEE303 pKa = 6.71GDD305 pKa = 3.74EE306 pKa = 4.15QVAEE310 pKa = 4.02YY311 pKa = 11.03SSFNSGVMTFNDD323 pKa = 3.47SSGLFSVSANGSKK336 pKa = 10.46VVTLKK341 pKa = 10.99NDD343 pKa = 3.3IASGTASGKK352 pKa = 7.07TFKK355 pKa = 10.78FSLGEE360 pKa = 4.07ASDD363 pKa = 4.01VSSDD367 pKa = 3.23ASEE370 pKa = 4.25VSGDD374 pKa = 3.4FALNGNTMTTANVDD388 pKa = 3.73DD389 pKa = 5.15LGQITVAGTSSSPTGTVDD407 pKa = 3.22PQDD410 pKa = 3.96DD411 pKa = 4.1YY412 pKa = 12.14EE413 pKa = 4.46VFNFNLQAGQQDD425 pKa = 3.88MEE427 pKa = 4.38VQKK430 pKa = 11.24LGFTNVGSTDD440 pKa = 3.4HH441 pKa = 7.28EE442 pKa = 4.95DD443 pKa = 3.28IQDD446 pKa = 3.38FEE448 pKa = 4.97LYY450 pKa = 10.85YY451 pKa = 11.08GGTKK455 pKa = 10.26LAGPMQMASDD465 pKa = 4.02DD466 pKa = 4.08TVSFDD471 pKa = 5.62LSDD474 pKa = 3.75SPII477 pKa = 4.04

Molecular weight: 50.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VPE1|A0A133VPE1_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382N08 OX=1698285 GN=AKJ56_01585 PE=4 SV=1
MM1 pKa = 7.32YY2 pKa = 8.87VTQTNFSARR11 pKa = 11.84EE12 pKa = 3.77LLKK15 pKa = 10.89VLFRR19 pKa = 11.84NRR21 pKa = 11.84FRR23 pKa = 11.84IVNRR27 pKa = 11.84TGSNVKK33 pKa = 9.87LRR35 pKa = 11.84YY36 pKa = 8.11EE37 pKa = 4.56HH38 pKa = 6.9PTNDD42 pKa = 3.34DD43 pKa = 3.18NNRR46 pKa = 11.84IVIVPMHH53 pKa = 6.98DD54 pKa = 3.78SIKK57 pKa = 9.91TGTLRR62 pKa = 11.84SIANQAGAKK71 pKa = 9.49NFQKK75 pKa = 10.56IKK77 pKa = 10.86DD78 pKa = 3.96WINQNII84 pKa = 3.45

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

431

0

431

101451

45

1209

235.4

26.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.618 ± 0.119

0.914 ± 0.052

5.817 ± 0.11

9.934 ± 0.203

4.047 ± 0.097

7.001 ± 0.134

1.461 ± 0.051

6.959 ± 0.112

8.603 ± 0.197

8.899 ± 0.153

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.06

4.589 ± 0.115

3.656 ± 0.062

2.965 ± 0.106

5.009 ± 0.117

6.528 ± 0.14

4.928 ± 0.124

6.508 ± 0.107

1.159 ± 0.044

3.27 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski