Callinectes sapidus associated circular virus
Average proteome isoelectric point is 8.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RLM8|A0A0K1RLM8_9CIRC Putative capsid protein OS=Callinectes sapidus associated circular virus OX=1692246 PE=4 SV=1
MM1 pKa = 7.46 TIYY4 pKa = 10.64 RR5 pKa = 11.84 EE6 pKa = 3.96 QSSAADD12 pKa = 3.36 WEE14 pKa = 4.24 RR15 pKa = 11.84 LKK17 pKa = 11.39 DD18 pKa = 3.41 KK19 pKa = 10.09 LTYY22 pKa = 9.63 FAYY25 pKa = 10.23 AEE27 pKa = 4.33 EE28 pKa = 4.57 TCPXTGRR35 pKa = 11.84 WHH37 pKa = 5.08 MQAYY41 pKa = 9.45 AYY43 pKa = 10.25 SRR45 pKa = 11.84 RR46 pKa = 11.84 AMRR49 pKa = 11.84 HH50 pKa = 4.12 SAWRR54 pKa = 11.84 KK55 pKa = 8.28 LFPKK59 pKa = 10.23 IHH61 pKa = 5.91 IEE63 pKa = 3.79 KK64 pKa = 9.43 MRR66 pKa = 11.84 GSFTDD71 pKa = 3.33 NEE73 pKa = 4.6 NYY75 pKa = 9.76 CSKK78 pKa = 10.42 EE79 pKa = 3.71 GQLIEE84 pKa = 4.4 FGEE87 pKa = 4.45 RR88 pKa = 11.84 PRR90 pKa = 11.84 QGEE93 pKa = 3.9 RR94 pKa = 11.84 SDD96 pKa = 3.5 LQEE99 pKa = 3.85 FKK101 pKa = 10.98 FRR103 pKa = 11.84 LDD105 pKa = 3.87 RR106 pKa = 11.84 GDD108 pKa = 3.66 KK109 pKa = 10.35 PLDD112 pKa = 3.11 IAYY115 pKa = 9.88 EE116 pKa = 4.09 DD117 pKa = 3.42 VSMFNAVGRR126 pKa = 11.84 MHH128 pKa = 7.36 RR129 pKa = 11.84 FAEE132 pKa = 4.81 TYY134 pKa = 9.27 SQYY137 pKa = 10.61 IRR139 pKa = 11.84 YY140 pKa = 8.75 RR141 pKa = 11.84 QIKK144 pKa = 9.08 DD145 pKa = 3.18 NRR147 pKa = 11.84 DD148 pKa = 3.22 APEE151 pKa = 3.9 VYY153 pKa = 10.43 LRR155 pKa = 11.84 IGPAGTGKK163 pKa = 8.47 TKK165 pKa = 10.11 WLDD168 pKa = 3.45 DD169 pKa = 3.96 TYY171 pKa = 10.96 GTAGYY176 pKa = 8.39 TIAPDD181 pKa = 3.48 NLGRR185 pKa = 11.84 WFDD188 pKa = 3.49 KK189 pKa = 10.83 CDD191 pKa = 3.87 CDD193 pKa = 4.76 VVLFDD198 pKa = 5.68 DD199 pKa = 4.6 VEE201 pKa = 5.37 AGQVPPFSLWKK212 pKa = 9.76 RR213 pKa = 11.84 LCDD216 pKa = 3.7 RR217 pKa = 11.84 YY218 pKa = 10.16 PIQVPVKK225 pKa = 10.62 GGFIWWKK232 pKa = 8.36 PKK234 pKa = 10.8 VIVFTSNQAPSKK246 pKa = 9.15 WWGEE250 pKa = 3.51 LDD252 pKa = 3.9 EE253 pKa = 4.8 LNKK256 pKa = 10.66 GAFDD260 pKa = 3.65 RR261 pKa = 11.84 RR262 pKa = 11.84 VTSIEE267 pKa = 3.99 VVEE270 pKa = 4.25
Molecular weight: 31.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.922
IPC2_protein 7.278
IPC_protein 7.293
Toseland 6.956
ProMoST 7.629
Dawson 7.951
Bjellqvist 8.156
Wikipedia 7.878
Rodwell 7.951
Grimsley 6.985
Solomon 8.053
Lehninger 8.068
Nozaki 8.317
DTASelect 8.024
Thurlkill 8.068
EMBOSS 8.17
Sillero 8.375
Patrickios 4.355
IPC_peptide 8.053
IPC2_peptide 7.293
IPC2.peptide.svr19 7.513
Protein with the highest isoelectric point:
>tr|A0A0K1RLM8|A0A0K1RLM8_9CIRC Putative capsid protein OS=Callinectes sapidus associated circular virus OX=1692246 PE=4 SV=1
MM1 pKa = 7.49 AYY3 pKa = 9.44 KK4 pKa = 10.26 RR5 pKa = 11.84 KK6 pKa = 9.82 RR7 pKa = 11.84 MPVRR11 pKa = 11.84 RR12 pKa = 11.84 FNKK15 pKa = 9.04 GRR17 pKa = 11.84 YY18 pKa = 5.5 TKK20 pKa = 10.33 RR21 pKa = 11.84 SRR23 pKa = 11.84 SRR25 pKa = 11.84 MTVNTVKK32 pKa = 10.68 KK33 pKa = 8.62 IAKK36 pKa = 8.42 SVVMRR41 pKa = 11.84 KK42 pKa = 9.24 VEE44 pKa = 4.13 PKK46 pKa = 10.1 HH47 pKa = 7.02 KK48 pKa = 10.56 SFNTGKK54 pKa = 10.61 YY55 pKa = 8.56 EE56 pKa = 4.44 LYY58 pKa = 10.41 HH59 pKa = 5.59 NTFNTYY65 pKa = 10.42 RR66 pKa = 11.84 INTDD70 pKa = 2.57 NQMPAEE76 pKa = 4.26 GTGDD80 pKa = 3.53 DD81 pKa = 3.76 QRR83 pKa = 11.84 IGDD86 pKa = 4.53 RR87 pKa = 11.84 IYY89 pKa = 8.31 TTGFKK94 pKa = 10.64 LRR96 pKa = 11.84 LLLGQKK102 pKa = 9.22 SDD104 pKa = 3.65 RR105 pKa = 11.84 PNVTFKK111 pKa = 10.55 IYY113 pKa = 10.71 VMKK116 pKa = 10.54 VPKK119 pKa = 10.12 GQGYY123 pKa = 10.19 AYY125 pKa = 10.48 ANFFSKK131 pKa = 9.89 MVPKK135 pKa = 10.3 HH136 pKa = 5.39 YY137 pKa = 9.75 RR138 pKa = 11.84 QCSTGG143 pKa = 3.17
Molecular weight: 16.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.293
IPC2_protein 10.131
IPC_protein 10.73
Toseland 10.818
ProMoST 10.467
Dawson 10.935
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 11.345
Grimsley 10.979
Solomon 11.008
Lehninger 10.979
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.818
EMBOSS 11.213
Sillero 10.847
Patrickios 11.052
IPC_peptide 11.008
IPC2_peptide 9.399
IPC2.peptide.svr19 8.187
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
413
143
270
206.5
24.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.569 ± 1.078
1.453 ± 0.392
6.295 ± 1.456
5.327 ± 1.679
5.085 ± 0.099
6.78 ± 0.111
1.695 ± 0.21
4.358 ± 0.448
8.717 ± 2.013
4.843 ± 0.7
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.148 ± 0.909
3.874 ± 1.258
4.6 ± 0.21
3.632 ± 0.07
9.443 ± 0.544
4.843 ± 0.027
6.053 ± 1.216
5.811 ± 0.615
2.421 ± 1.259
5.811 ± 0.615
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here