Anaerobacillus isosaccharinicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anaerobacillus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7S7L9A9|A0A7S7L9A9_9BACI Tyrosine-type recombinase/integrase OS=Anaerobacillus isosaccharinicus OX=1532552 GN=AWH56_003765 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.39KK3 pKa = 9.05ATRR6 pKa = 11.84LLTPALGVGLVFGAMGVSAQSEE28 pKa = 4.81TVTVEE33 pKa = 4.41SGDD36 pKa = 4.14TYY38 pKa = 10.84WGIAQGFDD46 pKa = 3.13GVTTEE51 pKa = 5.44DD52 pKa = 3.18LVEE55 pKa = 4.31ANEE58 pKa = 3.97YY59 pKa = 9.85NPRR62 pKa = 11.84AIPIGAEE69 pKa = 3.56IKK71 pKa = 10.59IPDD74 pKa = 4.42GEE76 pKa = 4.44TSSDD80 pKa = 3.31NDD82 pKa = 3.41VVTHH86 pKa = 6.51VIQPGNTLTNIAAVYY101 pKa = 10.58DD102 pKa = 4.08GVTVDD107 pKa = 5.96DD108 pKa = 5.23LFRR111 pKa = 11.84LNPGIDD117 pKa = 3.72PYY119 pKa = 11.46SLTVGSEE126 pKa = 3.99IVVVDD131 pKa = 3.55YY132 pKa = 11.33NSEE135 pKa = 3.92FGEE138 pKa = 4.61DD139 pKa = 3.74YY140 pKa = 10.57LYY142 pKa = 9.56HH143 pKa = 6.07TVQPGNTFNEE153 pKa = 4.18IASVYY158 pKa = 10.85DD159 pKa = 3.52GVTVDD164 pKa = 6.11DD165 pKa = 4.91LLEE168 pKa = 4.57ANPNEE173 pKa = 4.45DD174 pKa = 4.04PYY176 pKa = 11.49EE177 pKa = 3.91LTIGSRR183 pKa = 11.84IVIPLNN189 pKa = 3.43

Molecular weight: 20.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2MFI9|A0A1S2MFI9_9BACI 2-C-methyl-D-erythritol 2 4-cyclodiphosphate synthase OS=Anaerobacillus isosaccharinicus OX=1532552 GN=ispF PE=3 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.45GFRR20 pKa = 11.84ARR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.91VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5454

0

5454

1477624

26

1728

270.9

30.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.362 ± 0.039

0.823 ± 0.012

4.931 ± 0.022

7.609 ± 0.041

4.792 ± 0.028

6.514 ± 0.034

2.084 ± 0.015

8.21 ± 0.032

7.175 ± 0.036

10.015 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.014

4.708 ± 0.027

3.473 ± 0.023

3.674 ± 0.023

4.045 ± 0.025

5.95 ± 0.026

5.433 ± 0.023

6.996 ± 0.028

1.0 ± 0.013

3.569 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski