Vernonia yellow vein betasatellite
Average proteome isoelectric point is 9.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C9XIB2|C9XIB2_9VIRU C1 protein OS=Vernonia yellow vein betasatellite OX=666635 GN=C1 PE=4 SV=1
MM1 pKa = 7.43 TPLNTMRR8 pKa = 11.84 NNSRR12 pKa = 11.84 KK13 pKa = 6.78 THH15 pKa = 6.42 PKK17 pKa = 9.48 TMTIKK22 pKa = 9.18 HH23 pKa = 5.14 TTNKK27 pKa = 10.01 GIKK30 pKa = 8.57 FTIDD34 pKa = 2.83 AKK36 pKa = 11.11 LNEE39 pKa = 4.38 CFKK42 pKa = 10.82 IIVQVTRR49 pKa = 11.84 TNRR52 pKa = 11.84 NIITKK57 pKa = 8.45 HH58 pKa = 5.36 TYY60 pKa = 7.75 TMYY63 pKa = 10.54 YY64 pKa = 9.54 GYY66 pKa = 10.93 GAILVPFDD74 pKa = 4.09 FNGLEE79 pKa = 4.0 TQIRR83 pKa = 11.84 NTIEE87 pKa = 4.31 VMFHH91 pKa = 7.32 DD92 pKa = 4.32 IDD94 pKa = 3.73 HH95 pKa = 7.03 FKK97 pKa = 10.74 PEE99 pKa = 4.39 DD100 pKa = 3.47 MIGGNRR106 pKa = 11.84 CNHH109 pKa = 3.48 VRR111 pKa = 11.84 EE112 pKa = 4.25 YY113 pKa = 10.31 RR114 pKa = 11.84 FHH116 pKa = 7.68 RR117 pKa = 11.84 ITCTRR122 pKa = 11.84 TIQLL126 pKa = 3.67
Molecular weight: 14.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.156
IPC2_protein 9.194
IPC_protein 9.224
Toseland 9.853
ProMoST 9.575
Dawson 10.087
Bjellqvist 9.794
Wikipedia 10.248
Rodwell 10.438
Grimsley 10.16
Solomon 10.131
Lehninger 10.087
Nozaki 9.94
DTASelect 9.765
Thurlkill 9.926
EMBOSS 10.262
Sillero 10.014
Patrickios 9.926
IPC_peptide 10.116
IPC2_peptide 8.624
IPC2.peptide.svr19 8.044
Protein with the highest isoelectric point:
>tr|C9XIB2|C9XIB2_9VIRU C1 protein OS=Vernonia yellow vein betasatellite OX=666635 GN=C1 PE=4 SV=1
MM1 pKa = 7.43 TPLNTMRR8 pKa = 11.84 NNSRR12 pKa = 11.84 KK13 pKa = 6.78 THH15 pKa = 6.42 PKK17 pKa = 9.48 TMTIKK22 pKa = 9.18 HH23 pKa = 5.14 TTNKK27 pKa = 10.01 GIKK30 pKa = 8.57 FTIDD34 pKa = 2.83 AKK36 pKa = 11.11 LNEE39 pKa = 4.38 CFKK42 pKa = 10.82 IIVQVTRR49 pKa = 11.84 TNRR52 pKa = 11.84 NIITKK57 pKa = 8.45 HH58 pKa = 5.36 TYY60 pKa = 7.75 TMYY63 pKa = 10.54 YY64 pKa = 9.54 GYY66 pKa = 10.93 GAILVPFDD74 pKa = 4.09 FNGLEE79 pKa = 4.0 TQIRR83 pKa = 11.84 NTIEE87 pKa = 4.31 VMFHH91 pKa = 7.32 DD92 pKa = 4.32 IDD94 pKa = 3.73 HH95 pKa = 7.03 FKK97 pKa = 10.74 PEE99 pKa = 4.39 DD100 pKa = 3.47 MIGGNRR106 pKa = 11.84 CNHH109 pKa = 3.48 VRR111 pKa = 11.84 EE112 pKa = 4.25 YY113 pKa = 10.31 RR114 pKa = 11.84 FHH116 pKa = 7.68 RR117 pKa = 11.84 ITCTRR122 pKa = 11.84 TIQLL126 pKa = 3.67
Molecular weight: 14.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.156
IPC2_protein 9.194
IPC_protein 9.224
Toseland 9.853
ProMoST 9.575
Dawson 10.087
Bjellqvist 9.794
Wikipedia 10.248
Rodwell 10.438
Grimsley 10.16
Solomon 10.131
Lehninger 10.087
Nozaki 9.94
DTASelect 9.765
Thurlkill 9.926
EMBOSS 10.262
Sillero 10.014
Patrickios 9.926
IPC_peptide 10.116
IPC2_peptide 8.624
IPC2.peptide.svr19 8.044
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
126
126
126
126.0
14.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.587 ± 0.0
2.381 ± 0.0
3.968 ± 0.0
3.968 ± 0.0
5.556 ± 0.0
4.762 ± 0.0
5.556 ± 0.0
11.111 ± 0.0
7.143 ± 0.0
3.968 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.762 ± 0.0
8.73 ± 0.0
3.175 ± 0.0
2.381 ± 0.0
7.937 ± 0.0
0.794 ± 0.0
14.286 ± 0.0
3.968 ± 0.0
0.0 ± 0.0
3.968 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here