Alternaria arborescens mitovirus 1
Average proteome isoelectric point is 8.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4Z142|A0A1B4Z142_9VIRU Polyprotein OS=Alternaria arborescens mitovirus 1 OX=1826822 GN=RdRp PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.21 KK3 pKa = 9.72 QLYY6 pKa = 9.72 NITVKK11 pKa = 10.53 LCSIVFPTINTLDD24 pKa = 3.56 YY25 pKa = 10.76 LNPYY29 pKa = 9.63 FKK31 pKa = 11.02 LLNRR35 pKa = 11.84 LLNTQGLLKK44 pKa = 9.34 TVKK47 pKa = 10.17 YY48 pKa = 10.47 LKK50 pKa = 9.76 QCRR53 pKa = 11.84 LHH55 pKa = 5.93 CTRR58 pKa = 11.84 YY59 pKa = 8.52 MCGSPLLFNKK69 pKa = 10.25 LKK71 pKa = 10.6 IGLDD75 pKa = 3.14 TDD77 pKa = 3.99 GWPKK81 pKa = 10.66 RR82 pKa = 11.84 LDD84 pKa = 3.59 FLKK87 pKa = 10.63 PLAKK91 pKa = 10.42 GSLEE95 pKa = 3.74 QRR97 pKa = 11.84 KK98 pKa = 8.45 FLMTILCLSRR108 pKa = 11.84 TLKK111 pKa = 11.16 AEE113 pKa = 4.21 GKK115 pKa = 9.14 EE116 pKa = 3.88 KK117 pKa = 11.07 LKK119 pKa = 10.71 IKK121 pKa = 10.07 PDD123 pKa = 3.44 YY124 pKa = 11.16 DD125 pKa = 4.58 SITKK129 pKa = 9.36 PGRR132 pKa = 11.84 IIKK135 pKa = 7.5 TIPTGFIKK143 pKa = 10.6 EE144 pKa = 4.11 FVSNYY149 pKa = 7.24 NLKK152 pKa = 9.09 MEE154 pKa = 4.4 KK155 pKa = 10.07 PKK157 pKa = 11.03 FEE159 pKa = 4.59 INNIYY164 pKa = 10.32 LSNKK168 pKa = 9.31 AGPNGKK174 pKa = 8.33 ATKK177 pKa = 8.69 TAYY180 pKa = 10.3 SSLLSYY186 pKa = 10.73 SYY188 pKa = 11.69 DD189 pKa = 3.26 LMASLFKK196 pKa = 10.27 ITDD199 pKa = 3.28 QSGIDD204 pKa = 3.85 YY205 pKa = 9.08 FQSQYY210 pKa = 11.21 NYY212 pKa = 9.94 AWEE215 pKa = 4.42 KK216 pKa = 10.86 NFPSQKK222 pKa = 9.94 LGKK225 pKa = 10.2 LSFIYY230 pKa = 10.57 DD231 pKa = 3.99 PEE233 pKa = 5.39 CKK235 pKa = 9.98 LRR237 pKa = 11.84 IVAIVDD243 pKa = 3.55 YY244 pKa = 8.81 YY245 pKa = 10.63 TQLFLKK251 pKa = 9.58 PIHH254 pKa = 6.02 EE255 pKa = 5.03 KK256 pKa = 10.46 IMKK259 pKa = 9.78 KK260 pKa = 10.0 LQNLPCDD267 pKa = 3.41 RR268 pKa = 11.84 TYY270 pKa = 10.44 TQSPLNEE277 pKa = 4.12 WKK279 pKa = 10.5 DD280 pKa = 3.74 DD281 pKa = 3.79 GNMFWSIDD289 pKa = 3.64 LSSATDD295 pKa = 3.73 RR296 pKa = 11.84 FPISLQRR303 pKa = 11.84 RR304 pKa = 11.84 LLEE307 pKa = 3.68 IAISKK312 pKa = 9.5 EE313 pKa = 4.19 VADD316 pKa = 4.0 GWSFILSDD324 pKa = 4.22 RR325 pKa = 11.84 KK326 pKa = 10.6 FEE328 pKa = 4.23 TPEE331 pKa = 4.08 GNLVQYY337 pKa = 8.75 RR338 pKa = 11.84 TGQPMGSYY346 pKa = 10.31 SSWAAFTLTHH356 pKa = 6.43 HH357 pKa = 7.27 LVLHH361 pKa = 6.33 WCAKK365 pKa = 10.56 LNGIDD370 pKa = 3.91 NFSDD374 pKa = 4.14 YY375 pKa = 10.75 IILGDD380 pKa = 5.33 DD381 pKa = 3.38 IVIKK385 pKa = 10.17 NDD387 pKa = 2.96 KK388 pKa = 9.71 VARR391 pKa = 11.84 TYY393 pKa = 10.58 IKK395 pKa = 9.98 WMDD398 pKa = 3.62 YY399 pKa = 11.11 LGVEE403 pKa = 4.3 LSDD406 pKa = 3.97 SKK408 pKa = 9.83 THH410 pKa = 5.56 VSKK413 pKa = 10.14 DD414 pKa = 3.03 TYY416 pKa = 10.94 EE417 pKa = 3.89 FAKK420 pKa = 10.36 RR421 pKa = 11.84 WFCKK425 pKa = 10.08 GKK427 pKa = 10.14 EE428 pKa = 4.15 FTGLPMNGIVEE439 pKa = 4.51 NIEE442 pKa = 3.8 NPFIVMVNLYY452 pKa = 10.66 DD453 pKa = 4.32 FYY455 pKa = 11.25 KK456 pKa = 11.13 VKK458 pKa = 10.75 GNYY461 pKa = 9.56 LGSTKK466 pKa = 10.38 NLPCILSSLYY476 pKa = 10.47 KK477 pKa = 10.61 GLSLKK482 pKa = 10.54 LSKK485 pKa = 10.57 KK486 pKa = 9.94 FNNSRR491 pKa = 11.84 FKK493 pKa = 9.77 MKK495 pKa = 9.91 IYY497 pKa = 9.79 TFHH500 pKa = 7.43 KK501 pKa = 10.38 SLDD504 pKa = 3.51 YY505 pKa = 11.18 SFGYY509 pKa = 8.28 LTYY512 pKa = 10.88 DD513 pKa = 3.21 SLRR516 pKa = 11.84 EE517 pKa = 3.85 LLCLNIKK524 pKa = 10.17 NEE526 pKa = 3.86 QFMIPDD532 pKa = 3.41 EE533 pKa = 4.02 QLIHH537 pKa = 5.72 NTYY540 pKa = 11.11 DD541 pKa = 3.43 DD542 pKa = 3.83 VVAQGMGGSVKK553 pKa = 10.84 NSMTSLNNLASKK565 pKa = 10.59 VIEE568 pKa = 4.23 NKK570 pKa = 9.56 TIYY573 pKa = 10.51 NLEE576 pKa = 4.45 DD577 pKa = 3.88 PNEE580 pKa = 3.89 LRR582 pKa = 11.84 NYY584 pKa = 9.71 PIFKK588 pKa = 10.67 GIVNYY593 pKa = 10.1 INNYY597 pKa = 8.62 KK598 pKa = 10.75 DD599 pKa = 4.98 SVSKK603 pKa = 10.57 WDD605 pKa = 3.77 VNHH608 pKa = 6.85 LNYY611 pKa = 9.88 RR612 pKa = 11.84 QKK614 pKa = 10.97 SKK616 pKa = 10.91 EE617 pKa = 3.96 LLMLNIDD624 pKa = 3.56 NVFGKK629 pKa = 9.9 EE630 pKa = 3.82 RR631 pKa = 11.84 NKK633 pKa = 9.97 TLEE636 pKa = 4.08 LLNTGKK642 pKa = 10.05 IFSLGFKK649 pKa = 10.14 KK650 pKa = 10.48 INEE653 pKa = 4.37 TDD655 pKa = 3.31 EE656 pKa = 3.95 IMYY659 pKa = 10.18 GSSIGEE665 pKa = 3.9 STYY668 pKa = 11.2 SYY670 pKa = 11.65 NLDD673 pKa = 3.93 LFNLIQNNYY682 pKa = 10.45 SIDD685 pKa = 3.77 LKK687 pKa = 10.94 KK688 pKa = 10.83 LKK690 pKa = 10.4 EE691 pKa = 4.12 LDD693 pKa = 3.07 EE694 pKa = 4.52 GTYY697 pKa = 10.05 KK698 pKa = 10.81 EE699 pKa = 4.5 PVKK702 pKa = 9.75 QTPASAYY709 pKa = 9.62 DD710 pKa = 3.55 AYY712 pKa = 11.04 ANFFNN717 pKa = 5.07
Molecular weight: 83.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.553
IPC2_protein 8.536
IPC_protein 8.361
Toseland 8.99
ProMoST 8.902
Dawson 9.355
Bjellqvist 9.165
Wikipedia 9.531
Rodwell 9.56
Grimsley 9.414
Solomon 9.37
Lehninger 9.326
Nozaki 9.224
DTASelect 9.092
Thurlkill 9.209
EMBOSS 9.472
Sillero 9.341
Patrickios 4.317
IPC_peptide 9.37
IPC2_peptide 7.746
IPC2.peptide.svr19 7.679
Protein with the highest isoelectric point:
>tr|A0A1B4Z142|A0A1B4Z142_9VIRU Polyprotein OS=Alternaria arborescens mitovirus 1 OX=1826822 GN=RdRp PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.21 KK3 pKa = 9.72 QLYY6 pKa = 9.72 NITVKK11 pKa = 10.53 LCSIVFPTINTLDD24 pKa = 3.56 YY25 pKa = 10.76 LNPYY29 pKa = 9.63 FKK31 pKa = 11.02 LLNRR35 pKa = 11.84 LLNTQGLLKK44 pKa = 9.34 TVKK47 pKa = 10.17 YY48 pKa = 10.47 LKK50 pKa = 9.76 QCRR53 pKa = 11.84 LHH55 pKa = 5.93 CTRR58 pKa = 11.84 YY59 pKa = 8.52 MCGSPLLFNKK69 pKa = 10.25 LKK71 pKa = 10.6 IGLDD75 pKa = 3.14 TDD77 pKa = 3.99 GWPKK81 pKa = 10.66 RR82 pKa = 11.84 LDD84 pKa = 3.59 FLKK87 pKa = 10.63 PLAKK91 pKa = 10.42 GSLEE95 pKa = 3.74 QRR97 pKa = 11.84 KK98 pKa = 8.45 FLMTILCLSRR108 pKa = 11.84 TLKK111 pKa = 11.16 AEE113 pKa = 4.21 GKK115 pKa = 9.14 EE116 pKa = 3.88 KK117 pKa = 11.07 LKK119 pKa = 10.71 IKK121 pKa = 10.07 PDD123 pKa = 3.44 YY124 pKa = 11.16 DD125 pKa = 4.58 SITKK129 pKa = 9.36 PGRR132 pKa = 11.84 IIKK135 pKa = 7.5 TIPTGFIKK143 pKa = 10.6 EE144 pKa = 4.11 FVSNYY149 pKa = 7.24 NLKK152 pKa = 9.09 MEE154 pKa = 4.4 KK155 pKa = 10.07 PKK157 pKa = 11.03 FEE159 pKa = 4.59 INNIYY164 pKa = 10.32 LSNKK168 pKa = 9.31 AGPNGKK174 pKa = 8.33 ATKK177 pKa = 8.69 TAYY180 pKa = 10.3 SSLLSYY186 pKa = 10.73 SYY188 pKa = 11.69 DD189 pKa = 3.26 LMASLFKK196 pKa = 10.27 ITDD199 pKa = 3.28 QSGIDD204 pKa = 3.85 YY205 pKa = 9.08 FQSQYY210 pKa = 11.21 NYY212 pKa = 9.94 AWEE215 pKa = 4.42 KK216 pKa = 10.86 NFPSQKK222 pKa = 9.94 LGKK225 pKa = 10.2 LSFIYY230 pKa = 10.57 DD231 pKa = 3.99 PEE233 pKa = 5.39 CKK235 pKa = 9.98 LRR237 pKa = 11.84 IVAIVDD243 pKa = 3.55 YY244 pKa = 8.81 YY245 pKa = 10.63 TQLFLKK251 pKa = 9.58 PIHH254 pKa = 6.02 EE255 pKa = 5.03 KK256 pKa = 10.46 IMKK259 pKa = 9.78 KK260 pKa = 10.0 LQNLPCDD267 pKa = 3.41 RR268 pKa = 11.84 TYY270 pKa = 10.44 TQSPLNEE277 pKa = 4.12 WKK279 pKa = 10.5 DD280 pKa = 3.74 DD281 pKa = 3.79 GNMFWSIDD289 pKa = 3.64 LSSATDD295 pKa = 3.73 RR296 pKa = 11.84 FPISLQRR303 pKa = 11.84 RR304 pKa = 11.84 LLEE307 pKa = 3.68 IAISKK312 pKa = 9.5 EE313 pKa = 4.19 VADD316 pKa = 4.0 GWSFILSDD324 pKa = 4.22 RR325 pKa = 11.84 KK326 pKa = 10.6 FEE328 pKa = 4.23 TPEE331 pKa = 4.08 GNLVQYY337 pKa = 8.75 RR338 pKa = 11.84 TGQPMGSYY346 pKa = 10.31 SSWAAFTLTHH356 pKa = 6.43 HH357 pKa = 7.27 LVLHH361 pKa = 6.33 WCAKK365 pKa = 10.56 LNGIDD370 pKa = 3.91 NFSDD374 pKa = 4.14 YY375 pKa = 10.75 IILGDD380 pKa = 5.33 DD381 pKa = 3.38 IVIKK385 pKa = 10.17 NDD387 pKa = 2.96 KK388 pKa = 9.71 VARR391 pKa = 11.84 TYY393 pKa = 10.58 IKK395 pKa = 9.98 WMDD398 pKa = 3.62 YY399 pKa = 11.11 LGVEE403 pKa = 4.3 LSDD406 pKa = 3.97 SKK408 pKa = 9.83 THH410 pKa = 5.56 VSKK413 pKa = 10.14 DD414 pKa = 3.03 TYY416 pKa = 10.94 EE417 pKa = 3.89 FAKK420 pKa = 10.36 RR421 pKa = 11.84 WFCKK425 pKa = 10.08 GKK427 pKa = 10.14 EE428 pKa = 4.15 FTGLPMNGIVEE439 pKa = 4.51 NIEE442 pKa = 3.8 NPFIVMVNLYY452 pKa = 10.66 DD453 pKa = 4.32 FYY455 pKa = 11.25 KK456 pKa = 11.13 VKK458 pKa = 10.75 GNYY461 pKa = 9.56 LGSTKK466 pKa = 10.38 NLPCILSSLYY476 pKa = 10.47 KK477 pKa = 10.61 GLSLKK482 pKa = 10.54 LSKK485 pKa = 10.57 KK486 pKa = 9.94 FNNSRR491 pKa = 11.84 FKK493 pKa = 9.77 MKK495 pKa = 9.91 IYY497 pKa = 9.79 TFHH500 pKa = 7.43 KK501 pKa = 10.38 SLDD504 pKa = 3.51 YY505 pKa = 11.18 SFGYY509 pKa = 8.28 LTYY512 pKa = 10.88 DD513 pKa = 3.21 SLRR516 pKa = 11.84 EE517 pKa = 3.85 LLCLNIKK524 pKa = 10.17 NEE526 pKa = 3.86 QFMIPDD532 pKa = 3.41 EE533 pKa = 4.02 QLIHH537 pKa = 5.72 NTYY540 pKa = 11.11 DD541 pKa = 3.43 DD542 pKa = 3.83 VVAQGMGGSVKK553 pKa = 10.84 NSMTSLNNLASKK565 pKa = 10.59 VIEE568 pKa = 4.23 NKK570 pKa = 9.56 TIYY573 pKa = 10.51 NLEE576 pKa = 4.45 DD577 pKa = 3.88 PNEE580 pKa = 3.89 LRR582 pKa = 11.84 NYY584 pKa = 9.71 PIFKK588 pKa = 10.67 GIVNYY593 pKa = 10.1 INNYY597 pKa = 8.62 KK598 pKa = 10.75 DD599 pKa = 4.98 SVSKK603 pKa = 10.57 WDD605 pKa = 3.77 VNHH608 pKa = 6.85 LNYY611 pKa = 9.88 RR612 pKa = 11.84 QKK614 pKa = 10.97 SKK616 pKa = 10.91 EE617 pKa = 3.96 LLMLNIDD624 pKa = 3.56 NVFGKK629 pKa = 9.9 EE630 pKa = 3.82 RR631 pKa = 11.84 NKK633 pKa = 9.97 TLEE636 pKa = 4.08 LLNTGKK642 pKa = 10.05 IFSLGFKK649 pKa = 10.14 KK650 pKa = 10.48 INEE653 pKa = 4.37 TDD655 pKa = 3.31 EE656 pKa = 3.95 IMYY659 pKa = 10.18 GSSIGEE665 pKa = 3.9 STYY668 pKa = 11.2 SYY670 pKa = 11.65 NLDD673 pKa = 3.93 LFNLIQNNYY682 pKa = 10.45 SIDD685 pKa = 3.77 LKK687 pKa = 10.94 KK688 pKa = 10.83 LKK690 pKa = 10.4 EE691 pKa = 4.12 LDD693 pKa = 3.07 EE694 pKa = 4.52 GTYY697 pKa = 10.05 KK698 pKa = 10.81 EE699 pKa = 4.5 PVKK702 pKa = 9.75 QTPASAYY709 pKa = 9.62 DD710 pKa = 3.55 AYY712 pKa = 11.04 ANFFNN717 pKa = 5.07
Molecular weight: 83.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.553
IPC2_protein 8.536
IPC_protein 8.361
Toseland 8.99
ProMoST 8.902
Dawson 9.355
Bjellqvist 9.165
Wikipedia 9.531
Rodwell 9.56
Grimsley 9.414
Solomon 9.37
Lehninger 9.326
Nozaki 9.224
DTASelect 9.092
Thurlkill 9.209
EMBOSS 9.472
Sillero 9.341
Patrickios 4.317
IPC_peptide 9.37
IPC2_peptide 7.746
IPC2.peptide.svr19 7.679
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
717
717
717
717.0
83.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.068 ± 0.0
1.534 ± 0.0
5.718 ± 0.0
4.742 ± 0.0
5.021 ± 0.0
5.16 ± 0.0
1.255 ± 0.0
7.113 ± 0.0
10.739 ± 0.0
11.994 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.371 ± 0.0
7.671 ± 0.0
3.626 ± 0.0
2.789 ± 0.0
2.929 ± 0.0
7.531 ± 0.0
5.439 ± 0.0
3.626 ± 0.0
1.395 ± 0.0
6.276 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here