Amino acid dipepetide frequency for Kitasatospora sp. MMS16-BH015

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	24.577AlaAla: 24.577 ± 0.183	1.072AlaCys: 1.072 ± 0.02	8.154AlaAsp: 8.154 ± 0.072	10.175AlaGlu: 10.175 ± 0.133	3.536AlaPhe: 3.536 ± 0.037	14.584AlaGly: 14.584 ± 0.097	2.873AlaHis: 2.873 ± 0.036	3.298AlaIle: 3.298 ± 0.046	2.893AlaLys: 2.893 ± 0.053	15.509AlaLeu: 15.509 ± 0.112	2.307AlaMet: 2.307 ± 0.028	2.165AlaAsn: 2.165 ± 0.038	7.986AlaPro: 7.986 ± 0.079	4.177AlaGln: 4.177 ± 0.043	9.723AlaArg: 9.723 ± 0.092	5.464AlaSer: 5.464 ± 0.052	8.1AlaThr: 8.1 ± 0.072	12.759AlaVal: 12.759 ± 0.088	1.868AlaTrp: 1.868 ± 0.027	2.685AlaTyr: 2.685 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	1.109CysAla: 1.109 ± 0.022	0.087CysCys: 0.087 ± 0.006	0.429CysAsp: 0.429 ± 0.013	0.367CysGlu: 0.367 ± 0.012	0.233CysPhe: 0.233 ± 0.011	0.959CysGly: 0.959 ± 0.023	0.202CysHis: 0.202 ± 0.009	0.162CysIle: 0.162 ± 0.008	0.099CysLys: 0.099 ± 0.006	0.817CysLeu: 0.817 ± 0.019	0.115CysMet: 0.115 ± 0.006	0.155CysAsn: 0.155 ± 0.008	0.515CysPro: 0.515 ± 0.015	0.18CysGln: 0.18 ± 0.009	0.585CysArg: 0.585 ± 0.019	0.485CysSer: 0.485 ± 0.016	0.579CysThr: 0.579 ± 0.021	0.566CysVal: 0.566 ± 0.014	0.14CysTrp: 0.14 ± 0.007	0.192CysTyr: 0.192 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	6.295AspAla: 6.295 ± 0.056	0.436AspCys: 0.436 ± 0.012	2.368AspAsp: 2.368 ± 0.034	2.989AspGlu: 2.989 ± 0.041	1.508AspPhe: 1.508 ± 0.025	5.897AspGly: 5.897 ± 0.059	1.475AspHis: 1.475 ± 0.026	1.401AspIle: 1.401 ± 0.027	0.892AspLys: 0.892 ± 0.023	6.266AspLeu: 6.266 ± 0.055	0.565AspMet: 0.565 ± 0.013	0.925AspAsn: 0.925 ± 0.025	4.564AspPro: 4.564 ± 0.051	1.896AspGln: 1.896 ± 0.031	4.602AspArg: 4.602 ± 0.053	2.534AspSer: 2.534 ± 0.033	3.044AspThr: 3.044 ± 0.047	3.425AspVal: 3.425 ± 0.038	0.994AspTrp: 0.994 ± 0.021	1.164AspTyr: 1.164 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.477GluAla: 7.477 ± 0.097	0.348GluCys: 0.348 ± 0.013	2.412GluAsp: 2.412 ± 0.035	3.382GluGlu: 3.382 ± 0.049	1.425GluPhe: 1.425 ± 0.024	3.991GluGly: 3.991 ± 0.042	1.536GluHis: 1.536 ± 0.029	1.996GluIle: 1.996 ± 0.031	0.992GluLys: 0.992 ± 0.025	7.903GluLeu: 7.903 ± 0.087	0.667GluMet: 0.667 ± 0.016	0.828GluAsn: 0.828 ± 0.018	3.225GluPro: 3.225 ± 0.045	2.61GluGln: 2.61 ± 0.037	4.957GluArg: 4.957 ± 0.06	2.253GluSer: 2.253 ± 0.032	2.733GluThr: 2.733 ± 0.04	4.387GluVal: 4.387 ± 0.05	0.719GluTrp: 0.719 ± 0.018	1.169GluTyr: 1.169 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.736PheAla: 3.736 ± 0.044	0.273PheCys: 0.273 ± 0.011	1.854PheAsp: 1.854 ± 0.03	1.422PheGlu: 1.422 ± 0.023	0.807PhePhe: 0.807 ± 0.022	3.02PheGly: 3.02 ± 0.042	0.616PheHis: 0.616 ± 0.015	0.619PheIle: 0.619 ± 0.017	0.456PheLys: 0.456 ± 0.015	2.494PheLeu: 2.494 ± 0.037	0.328PheMet: 0.328 ± 0.013	0.59PheAsn: 0.59 ± 0.017	1.344PhePro: 1.344 ± 0.023	0.719PheGln: 0.719 ± 0.018	1.682PheArg: 1.682 ± 0.027	1.349PheSer: 1.349 ± 0.023	2.055PheThr: 2.055 ± 0.034	1.946PheVal: 1.946 ± 0.033	0.394PheTrp: 0.394 ± 0.013	0.551PheTyr: 0.551 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.814GlyAla: 11.814 ± 0.1	0.902GlyCys: 0.902 ± 0.024	4.381GlyAsp: 4.381 ± 0.046	5.081GlyGlu: 5.081 ± 0.056	2.781GlyPhe: 2.781 ± 0.042	9.254GlyGly: 9.254 ± 0.097	2.374GlyHis: 2.374 ± 0.031	3.077GlyIle: 3.077 ± 0.039	2.208GlyLys: 2.208 ± 0.04	10.567GlyLeu: 10.567 ± 0.081	1.772GlyMet: 1.772 ± 0.028	1.794GlyAsn: 1.794 ± 0.034	5.725GlyPro: 5.725 ± 0.066	3.147GlyGln: 3.147 ± 0.042	7.714GlyArg: 7.714 ± 0.072	5.776GlySer: 5.776 ± 0.066	6.48GlyThr: 6.48 ± 0.085	7.312GlyVal: 7.312 ± 0.069	1.841GlyTrp: 1.841 ± 0.03	2.588GlyTyr: 2.588 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.576HisAla: 2.576 ± 0.034	0.24HisCys: 0.24 ± 0.009	1.138HisAsp: 1.138 ± 0.02	1.101HisGlu: 1.101 ± 0.025	0.609HisPhe: 0.609 ± 0.016	2.417HisGly: 2.417 ± 0.033	0.788HisHis: 0.788 ± 0.02	0.505HisIle: 0.505 ± 0.015	0.297HisLys: 0.297 ± 0.012	2.759HisLeu: 2.759 ± 0.046	0.284HisMet: 0.284 ± 0.012	0.378HisAsn: 0.378 ± 0.012	2.101HisPro: 2.101 ± 0.036	0.805HisGln: 0.805 ± 0.018	2.319HisArg: 2.319 ± 0.031	1.092HisSer: 1.092 ± 0.022	1.304HisThr: 1.304 ± 0.024	1.422HisVal: 1.422 ± 0.024	0.415HisTrp: 0.415 ± 0.013	0.505HisTyr: 0.505 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.435IleAla: 4.435 ± 0.045	0.285IleCys: 0.285 ± 0.012	1.957IleAsp: 1.957 ± 0.034	1.816IleGlu: 1.816 ± 0.03	0.6IlePhe: 0.6 ± 0.012	3.425IleGly: 3.425 ± 0.038	0.555IleHis: 0.555 ± 0.016	0.735IleIle: 0.735 ± 0.022	0.636IleLys: 0.636 ± 0.017	2.118IleLeu: 2.118 ± 0.035	0.346IleMet: 0.346 ± 0.013	0.629IleAsn: 0.629 ± 0.018	1.641IlePro: 1.641 ± 0.028	0.72IleGln: 0.72 ± 0.017	2.013IleArg: 2.013 ± 0.03	1.605IleSer: 1.605 ± 0.025	2.156IleThr: 2.156 ± 0.034	2.211IleVal: 2.211 ± 0.032	0.346IleTrp: 0.346 ± 0.013	0.457IleTyr: 0.457 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.709LysAla: 2.709 ± 0.046	0.105LysCys: 0.105 ± 0.006	1.051LysAsp: 1.051 ± 0.025	0.896LysGlu: 0.896 ± 0.021	0.407LysPhe: 0.407 ± 0.013	1.547LysGly: 1.547 ± 0.029	0.411LysHis: 0.411 ± 0.014	0.742LysIle: 0.742 ± 0.02	0.554LysLys: 0.554 ± 0.024	1.976LysLeu: 1.976 ± 0.034	0.312LysMet: 0.312 ± 0.012	0.428LysAsn: 0.428 ± 0.017	1.296LysPro: 1.296 ± 0.031	0.658LysGln: 0.658 ± 0.018	1.099LysArg: 1.099 ± 0.021	1.025LysSer: 1.025 ± 0.029	1.192LysThr: 1.192 ± 0.027	1.741LysVal: 1.741 ± 0.032	0.228LysTrp: 0.228 ± 0.01	0.458LysTyr: 0.458 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	17.885LeuAla: 17.885 ± 0.138	0.893LeuCys: 0.893 ± 0.019	6.688LeuAsp: 6.688 ± 0.063	4.409LeuGlu: 4.409 ± 0.051	2.584LeuPhe: 2.584 ± 0.039	10.28LeuGly: 10.28 ± 0.084	2.507LeuHis: 2.507 ± 0.033	3.149LeuIle: 3.149 ± 0.046	1.821LeuLys: 1.821 ± 0.03	12.787LeuLeu: 12.787 ± 0.122	1.421LeuMet: 1.421 ± 0.024	1.731LeuAsn: 1.731 ± 0.028	7.353LeuPro: 7.353 ± 0.061	2.36LeuGln: 2.36 ± 0.031	8.9LeuArg: 8.9 ± 0.084	5.227LeuSer: 5.227 ± 0.052	7.739LeuThr: 7.739 ± 0.063	9.157LeuVal: 9.157 ± 0.075	1.324LeuTrp: 1.324 ± 0.027	1.844LeuTyr: 1.844 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	1.982MetAla: 1.982 ± 0.031	0.105MetCys: 0.105 ± 0.007	0.713MetAsp: 0.713 ± 0.017	0.549MetGlu: 0.549 ± 0.014	0.371MetPhe: 0.371 ± 0.013	1.102MetGly: 1.102 ± 0.024	0.309MetHis: 0.309 ± 0.01	0.572MetIle: 0.572 ± 0.016	0.334MetLys: 0.334 ± 0.011	1.52MetLeu: 1.52 ± 0.028	0.254MetMet: 0.254 ± 0.01	0.379MetAsn: 0.379 ± 0.012	0.976MetPro: 0.976 ± 0.018	0.388MetGln: 0.388 ± 0.012	1.088MetArg: 1.088 ± 0.019	1.142MetSer: 1.142 ± 0.023	1.41MetThr: 1.41 ± 0.026	1.168MetVal: 1.168 ± 0.025	0.157MetTrp: 0.157 ± 0.008	0.274MetTyr: 0.274 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.128AsnAla: 2.128 ± 0.034	0.17AsnCys: 0.17 ± 0.009	0.88AsnAsp: 0.88 ± 0.02	0.749AsnGlu: 0.749 ± 0.019	0.508AsnPhe: 0.508 ± 0.017	2.152AsnGly: 2.152 ± 0.045	0.39AsnHis: 0.39 ± 0.012	0.551AsnIle: 0.551 ± 0.016	0.327AsnLys: 0.327 ± 0.013	1.787AsnLeu: 1.787 ± 0.032	0.255AsnMet: 0.255 ± 0.01	0.479AsnAsn: 0.479 ± 0.025	1.408AsnPro: 1.408 ± 0.03	0.614AsnGln: 0.614 ± 0.019	1.16AsnArg: 1.16 ± 0.026	1.088AsnSer: 1.088 ± 0.027	1.214AsnThr: 1.214 ± 0.034	1.291AsnVal: 1.291 ± 0.03	0.296AsnTrp: 0.296 ± 0.011	0.415AsnTyr: 0.415 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	10.742ProAla: 10.742 ± 0.089	0.354ProCys: 0.354 ± 0.012	3.998ProAsp: 3.998 ± 0.044	4.468ProGlu: 4.468 ± 0.055	1.512ProPhe: 1.512 ± 0.024	7.308ProGly: 7.308 ± 0.07	1.336ProHis: 1.336 ± 0.025	1.38ProIle: 1.38 ± 0.024	1.172ProLys: 1.172 ± 0.026	5.641ProLeu: 5.641 ± 0.055	0.885ProMet: 0.885 ± 0.02	1.051ProAsn: 1.051 ± 0.033	3.596ProPro: 3.596 ± 0.058	1.961ProGln: 1.961 ± 0.031	3.479ProArg: 3.479 ± 0.04	3.293ProSer: 3.293 ± 0.052	4.086ProThr: 4.086 ± 0.075	5.864ProVal: 5.864 ± 0.057	0.923ProTrp: 0.923 ± 0.02	1.414ProTyr: 1.414 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	4.42GlnAla: 4.42 ± 0.046	0.177GlnCys: 0.177 ± 0.008	1.374GlnAsp: 1.374 ± 0.026	1.409GlnGlu: 1.409 ± 0.025	0.645GlnPhe: 0.645 ± 0.017	2.453GlnGly: 2.453 ± 0.035	0.729GlnHis: 0.729 ± 0.016	1.027GlnIle: 1.027 ± 0.023	0.51GlnLys: 0.51 ± 0.018	3.91GlnLeu: 3.91 ± 0.041	0.399GlnMet: 0.399 ± 0.013	0.532GlnAsn: 0.532 ± 0.014	2.109GlnPro: 2.109 ± 0.036	1.573GlnGln: 1.573 ± 0.035	2.426GlnArg: 2.426 ± 0.036	1.371GlnSer: 1.371 ± 0.024	1.643GlnThr: 1.643 ± 0.033	2.696GlnVal: 2.696 ± 0.035	0.501GlnTrp: 0.501 ± 0.017	0.705GlnTyr: 0.705 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.776ArgAla: 9.776 ± 0.086	0.562ArgCys: 0.562 ± 0.014	3.265ArgAsp: 3.265 ± 0.044	4.399ArgGlu: 4.399 ± 0.054	2.087ArgPhe: 2.087 ± 0.033	5.238ArgGly: 5.238 ± 0.055	2.02ArgHis: 2.02 ± 0.03	2.898ArgIle: 2.898 ± 0.041	1.31ArgLys: 1.31 ± 0.03	9.058ArgLeu: 9.058 ± 0.088	1.446ArgMet: 1.446 ± 0.025	1.183ArgAsn: 1.183 ± 0.024	5.188ArgPro: 5.188 ± 0.054	2.348ArgGln: 2.348 ± 0.033	7.232ArgArg: 7.232 ± 0.079	3.987ArgSer: 3.987 ± 0.042	4.97ArgThr: 4.97 ± 0.047	5.302ArgVal: 5.302 ± 0.052	1.354ArgTrp: 1.354 ± 0.026	1.862ArgTyr: 1.862 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	7.051SerAla: 7.051 ± 0.064	0.488SerCys: 0.488 ± 0.015	2.261SerAsp: 2.261 ± 0.044	2.074SerGlu: 2.074 ± 0.029	1.579SerPhe: 1.579 ± 0.027	5.932SerGly: 5.932 ± 0.067	1.023SerHis: 1.023 ± 0.021	1.42SerIle: 1.42 ± 0.027	0.875SerLys: 0.875 ± 0.019	4.757SerLeu: 4.757 ± 0.047	0.969SerMet: 0.969 ± 0.018	0.937SerAsn: 0.937 ± 0.025	3.349SerPro: 3.349 ± 0.044	1.269SerGln: 1.269 ± 0.03	3.293SerArg: 3.293 ± 0.037	3.011SerSer: 3.011 ± 0.053	3.43SerThr: 3.43 ± 0.048	4.107SerVal: 4.107 ± 0.046	0.995SerTrp: 0.995 ± 0.023	1.363SerTyr: 1.363 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	10.192ThrAla: 10.192 ± 0.085	0.433ThrCys: 0.433 ± 0.017	3.669ThrAsp: 3.669 ± 0.048	3.343ThrGlu: 3.343 ± 0.039	1.619ThrPhe: 1.619 ± 0.026	7.178ThrGly: 7.178 ± 0.081	1.179ThrHis: 1.179 ± 0.026	1.647ThrIle: 1.647 ± 0.03	1.165ThrLys: 1.165 ± 0.029	6.041ThrLeu: 6.041 ± 0.052	0.861ThrMet: 0.861 ± 0.018	1.153ThrAsn: 1.153 ± 0.032	4.864ThrPro: 4.864 ± 0.077	1.432ThrGln: 1.432 ± 0.028	3.659ThrArg: 3.659 ± 0.046	3.133ThrSer: 3.133 ± 0.048	4.47ThrThr: 4.47 ± 0.066	6.483ThrVal: 6.483 ± 0.078	0.868ThrTrp: 0.868 ± 0.021	1.302ThrTyr: 1.302 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	10.519ValAla: 10.519 ± 0.083	0.675ValCys: 0.675 ± 0.017	4.594ValAsp: 4.594 ± 0.04	4.646ValGlu: 4.646 ± 0.052	2.309ValPhe: 2.309 ± 0.037	6.48ValGly: 6.48 ± 0.066	1.882ValHis: 1.882 ± 0.03	2.629ValIle: 2.629 ± 0.035	1.665ValLys: 1.665 ± 0.038	9.855ValLeu: 9.855 ± 0.074	1.171ValMet: 1.171 ± 0.023	1.673ValAsn: 1.673 ± 0.031	5.119ValPro: 5.119 ± 0.055	2.192ValGln: 2.192 ± 0.034	6.282ValArg: 6.282 ± 0.058	4.317ValSer: 4.317 ± 0.046	5.751ValThr: 5.751 ± 0.071	7.435ValVal: 7.435 ± 0.072	1.049ValTrp: 1.049 ± 0.018	1.542ValTyr: 1.542 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.772TrpAla: 1.772 ± 0.032	0.165TrpCys: 0.165 ± 0.007	0.711TrpAsp: 0.711 ± 0.019	0.68TrpGlu: 0.68 ± 0.017	0.455TrpPhe: 0.455 ± 0.014	1.062TrpGly: 1.062 ± 0.023	0.393TrpHis: 0.393 ± 0.014	0.497TrpIle: 0.497 ± 0.016	0.296TrpLys: 0.296 ± 0.011	1.822TrpLeu: 1.822 ± 0.037	0.239TrpMet: 0.239 ± 0.009	0.372TrpAsn: 0.372 ± 0.014	0.85TrpPro: 0.85 ± 0.019	0.732TrpGln: 0.732 ± 0.019	1.273TrpArg: 1.273 ± 0.023	1.008TrpSer: 1.008 ± 0.018	1.054TrpThr: 1.054 ± 0.023	0.996TrpVal: 0.996 ± 0.021	0.331TrpTrp: 0.331 ± 0.013	0.42TrpTyr: 0.42 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.714TyrAla: 2.714 ± 0.033	0.207TyrCys: 0.207 ± 0.008	1.341TyrAsp: 1.341 ± 0.031	1.057TyrGlu: 1.057 ± 0.02	0.676TyrPhe: 0.676 ± 0.017	2.308TyrGly: 2.308 ± 0.035	0.458TyrHis: 0.458 ± 0.014	0.379TyrIle: 0.379 ± 0.012	0.331TyrLys: 0.331 ± 0.015	2.47TyrLeu: 2.47 ± 0.034	0.2TyrMet: 0.2 ± 0.01	0.48TyrAsn: 0.48 ± 0.016	1.257TyrPro: 1.257 ± 0.021	0.831TyrGln: 0.831 ± 0.02	2.0TyrArg: 2.0 ± 0.03	1.022TyrSer: 1.022 ± 0.023	1.281TyrThr: 1.281 ± 0.029	1.515TyrVal: 1.515 ± 0.024	0.384TyrTrp: 0.384 ± 0.015	0.492TyrTyr: 0.492 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7193 proteins (2544074 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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