Paraphotobacterium marinum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Paraphotobacterium

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2308 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220VCV3|A0A220VCV3_9GAMM Diaminopimelate epimerase OS=Paraphotobacterium marinum OX=1755811 GN=dapF PE=3 SV=1
MM1 pKa = 7.96EE2 pKa = 5.19YY3 pKa = 8.77NTSEE7 pKa = 3.68ICDD10 pKa = 3.33IYY12 pKa = 10.74LDD14 pKa = 3.65QVDD17 pKa = 4.1VVEE20 pKa = 5.59PIFNSYY26 pKa = 9.92GGRR29 pKa = 11.84DD30 pKa = 3.46SFAGIITTVKK40 pKa = 10.3CFEE43 pKa = 5.06DD44 pKa = 3.65NQVLYY49 pKa = 11.09NVLNEE54 pKa = 4.62DD55 pKa = 3.04GSGRR59 pKa = 11.84VLLIDD64 pKa = 4.1AGGSLRR70 pKa = 11.84KK71 pKa = 9.85AILDD75 pKa = 3.34IDD77 pKa = 4.21LVEE80 pKa = 4.72LASDD84 pKa = 3.96NQWEE88 pKa = 4.85GIVVYY93 pKa = 10.54GCVRR97 pKa = 11.84NVDD100 pKa = 4.49EE101 pKa = 4.78IQHH104 pKa = 5.98LNIGIQALTSIPVLADD120 pKa = 3.26NQGTGEE126 pKa = 3.99IDD128 pKa = 3.48VPVNFGGVTFLPEE141 pKa = 4.77DD142 pKa = 3.66YY143 pKa = 10.64LYY145 pKa = 11.29ADD147 pKa = 3.41NTGIILSPEE156 pKa = 4.08ALDD159 pKa = 5.17LEE161 pKa = 4.73

Molecular weight: 17.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220VCS4|A0A220VCS4_9GAMM Glycine--tRNA ligase beta subunit OS=Paraphotobacterium marinum OX=1755811 GN=glyS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.36RR14 pKa = 11.84SHH16 pKa = 6.13GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.92NGQRR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.9GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2308

0

2308

704008

38

5776

305.0

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.362 ± 0.053

1.126 ± 0.026

5.739 ± 0.346

5.534 ± 0.06

5.264 ± 0.062

5.706 ± 0.074

1.935 ± 0.028

9.136 ± 0.105

8.113 ± 0.105

10.258 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.033

6.876 ± 0.065

3.301 ± 0.035

3.545 ± 0.045

3.07 ± 0.042

7.46 ± 0.058

5.111 ± 0.083

5.611 ± 0.062

0.961 ± 0.023

3.665 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski