Blastococcus sp. TF02A-30

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3930 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367ALH2|A0A367ALH2_9ACTN NADP-dependent oxidoreductase OS=Blastococcus sp. TF02A-30 OX=2250580 GN=DQ241_08025 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 9.25LTKK5 pKa = 10.11RR6 pKa = 11.84RR7 pKa = 11.84GAFVATALSAALVLSACGGGDD28 pKa = 4.33DD29 pKa = 4.44EE30 pKa = 6.67GGDD33 pKa = 3.79GGSASGGGEE42 pKa = 3.33FSIYY46 pKa = 10.23IGDD49 pKa = 4.11PEE51 pKa = 4.44NPLIPGNTTEE61 pKa = 4.32TEE63 pKa = 4.04GGQVVDD69 pKa = 4.19TLWTGLVTYY78 pKa = 10.28DD79 pKa = 4.41HH80 pKa = 6.69EE81 pKa = 4.63TSEE84 pKa = 4.21ATFDD88 pKa = 3.76GVAEE92 pKa = 4.58SIEE95 pKa = 4.78SDD97 pKa = 4.19DD98 pKa = 3.55QTTWTVTLKK107 pKa = 10.95DD108 pKa = 2.87GWTFHH113 pKa = 7.74DD114 pKa = 4.11GTPVNAEE121 pKa = 3.95SFVNAWNWNAYY132 pKa = 9.45SPNAAGNSYY141 pKa = 10.31FFANIEE147 pKa = 4.49GYY149 pKa = 10.57DD150 pKa = 3.62EE151 pKa = 4.4LQAEE155 pKa = 4.6EE156 pKa = 5.11GGTPAADD163 pKa = 3.22AMSGLTVVDD172 pKa = 4.43DD173 pKa = 3.81LTFEE177 pKa = 4.58VKK179 pKa = 10.67LSAPYY184 pKa = 9.87AQWPTTVGYY193 pKa = 7.89TAFFPLPEE201 pKa = 4.67AFFDD205 pKa = 4.02DD206 pKa = 4.57PEE208 pKa = 5.77AFGSQPIGNGPFQAEE223 pKa = 4.13EE224 pKa = 4.18PFIEE228 pKa = 4.74GQGITLTKK236 pKa = 10.34FEE238 pKa = 5.52DD239 pKa = 4.07YY240 pKa = 10.93AGEE243 pKa = 4.67DD244 pKa = 3.6EE245 pKa = 4.6AQADD249 pKa = 3.67AVRR252 pKa = 11.84YY253 pKa = 9.09VVYY256 pKa = 10.47TEE258 pKa = 5.6LNTAYY263 pKa = 9.57TDD265 pKa = 3.93LQGGTLDD272 pKa = 4.89IVDD275 pKa = 4.86TLPPDD280 pKa = 5.12AIASAEE286 pKa = 4.15GEE288 pKa = 4.0FGDD291 pKa = 5.31RR292 pKa = 11.84YY293 pKa = 10.52IEE295 pKa = 4.07TAQGDD300 pKa = 3.84ITSLGFPTYY309 pKa = 10.13DD310 pKa = 4.1EE311 pKa = 5.18RR312 pKa = 11.84FADD315 pKa = 3.77PNVRR319 pKa = 11.84KK320 pKa = 9.59AFSMAIDD327 pKa = 3.64RR328 pKa = 11.84QSISDD333 pKa = 4.15AIFNGTRR340 pKa = 11.84TPATSYY346 pKa = 10.67VSPVVDD352 pKa = 4.93GYY354 pKa = 11.58RR355 pKa = 11.84DD356 pKa = 3.78DD357 pKa = 4.76ACEE360 pKa = 3.97ACEE363 pKa = 5.31LDD365 pKa = 3.31VDD367 pKa = 4.07EE368 pKa = 5.97ANRR371 pKa = 11.84LLDD374 pKa = 3.33EE375 pKa = 5.15AGFDD379 pKa = 3.36RR380 pKa = 11.84TQPVDD385 pKa = 2.88LWFNAGGGHH394 pKa = 5.67EE395 pKa = 4.32EE396 pKa = 3.85WMQAVGNQIRR406 pKa = 11.84TNLGVEE412 pKa = 3.95YY413 pKa = 10.09QLRR416 pKa = 11.84GDD418 pKa = 3.92LQFAEE423 pKa = 4.45YY424 pKa = 10.73LPLQDD429 pKa = 3.88AQGMTGPFRR438 pKa = 11.84SGWIMDD444 pKa = 3.89YY445 pKa = 11.05PVMEE449 pKa = 4.64NFLGPLYY456 pKa = 9.91STAALPPAGSNVTFYY471 pKa = 11.55SNPEE475 pKa = 3.17FDD477 pKa = 4.29AALEE481 pKa = 4.48AGNSAASIDD490 pKa = 4.02EE491 pKa = 5.35AITAYY496 pKa = 10.51QEE498 pKa = 4.42AEE500 pKa = 4.59DD501 pKa = 4.51ILLEE505 pKa = 4.54DD506 pKa = 5.34FPAAPLFYY514 pKa = 10.52RR515 pKa = 11.84LNQGAHH521 pKa = 5.34SEE523 pKa = 4.19RR524 pKa = 11.84VSNVIINAFGRR535 pKa = 11.84IDD537 pKa = 3.43AAAVTVNDD545 pKa = 3.78

Molecular weight: 58.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A367AV44|A0A367AV44_9ACTN Uncharacterized protein OS=Blastococcus sp. TF02A-30 OX=2250580 GN=DQ241_06275 PE=4 SV=1
MM1 pKa = 6.81PTRR4 pKa = 11.84WGRR7 pKa = 11.84GRR9 pKa = 11.84GGPPGRR15 pKa = 11.84AAPSGSAPGGPCGAPRR31 pKa = 11.84RR32 pKa = 11.84AAAAGARR39 pKa = 11.84PGRR42 pKa = 11.84RR43 pKa = 11.84GPGRR47 pKa = 11.84RR48 pKa = 11.84SGPPASRR55 pKa = 11.84GPARR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84GRR63 pKa = 11.84PGALPP68 pKa = 3.66

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3930

0

3930

1241105

24

2049

315.8

33.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.43 ± 0.061

0.66 ± 0.011

6.228 ± 0.033

5.759 ± 0.041

2.574 ± 0.022

9.67 ± 0.042

1.975 ± 0.018

2.817 ± 0.029

1.435 ± 0.023

10.799 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.591 ± 0.013

1.424 ± 0.021

6.182 ± 0.036

2.726 ± 0.021

8.22 ± 0.049

4.698 ± 0.026

5.81 ± 0.029

9.817 ± 0.04

1.477 ± 0.017

1.709 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski