Acinetobacter phage fEg-Aba01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5C7D7|A0A6M5C7D7_9CAUD Integrase OS=Acinetobacter phage fEg-Aba01 OX=2736181 GN=fEgAba01_25 PE=3 SV=1
MM1 pKa = 7.07VKK3 pKa = 10.37VPIFKK8 pKa = 10.47LARR11 pKa = 11.84ADD13 pKa = 3.76PAVTALLEE21 pKa = 4.53SNGILRR27 pKa = 11.84VWKK30 pKa = 10.15FGHH33 pKa = 7.04APDD36 pKa = 5.7DD37 pKa = 4.03PQTPYY42 pKa = 9.69VTWQTITGDD51 pKa = 3.81SNSNLDD57 pKa = 3.71SRR59 pKa = 11.84PVSDD63 pKa = 4.18NAIIQIDD70 pKa = 4.29VYY72 pKa = 10.9ATDD75 pKa = 3.7EE76 pKa = 4.28DD77 pKa = 4.38VVEE80 pKa = 4.42QVAIAMRR87 pKa = 11.84DD88 pKa = 3.74AIEE91 pKa = 4.12LDD93 pKa = 4.18CYY95 pKa = 8.73VVRR98 pKa = 11.84YY99 pKa = 10.3GEE101 pKa = 4.11ADD103 pKa = 3.48KK104 pKa = 11.52DD105 pKa = 3.93PVTGMPHH112 pKa = 5.91YY113 pKa = 10.74SFDD116 pKa = 3.49VSWIVNRR123 pKa = 11.84EE124 pKa = 3.69

Molecular weight: 13.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5CAY2|A0A6M5CAY2_9CAUD Cro repressor OS=Acinetobacter phage fEg-Aba01 OX=2736181 GN=fEgAba01_33 PE=4 SV=1
MM1 pKa = 7.35ATQIHH6 pKa = 6.05GLEE9 pKa = 3.91PALRR13 pKa = 11.84KK14 pKa = 8.42MQAIGNEE21 pKa = 4.14KK22 pKa = 8.1TVKK25 pKa = 10.3RR26 pKa = 11.84IARR29 pKa = 11.84KK30 pKa = 9.94AMRR33 pKa = 11.84QAMNIARR40 pKa = 11.84DD41 pKa = 3.54EE42 pKa = 4.03ARR44 pKa = 11.84QKK46 pKa = 10.75VKK48 pKa = 10.78RR49 pKa = 11.84LDD51 pKa = 4.27DD52 pKa = 3.68PTTPEE57 pKa = 4.81KK58 pKa = 9.61IWKK61 pKa = 9.51EE62 pKa = 3.47IVVQNGRR69 pKa = 11.84SRR71 pKa = 11.84NKK73 pKa = 7.96NTLVMRR79 pKa = 11.84VGVRR83 pKa = 11.84GGARR87 pKa = 11.84IPYY90 pKa = 8.42TNNAQNRR97 pKa = 11.84RR98 pKa = 11.84SGRR101 pKa = 11.84VGKK104 pKa = 8.62TYY106 pKa = 9.25QTDD109 pKa = 2.9GRR111 pKa = 11.84VFYY114 pKa = 10.44WRR116 pKa = 11.84FLEE119 pKa = 5.09LGTSRR124 pKa = 11.84QPATPFLRR132 pKa = 11.84PALYY136 pKa = 10.69EE137 pKa = 4.22NIEE140 pKa = 4.18QITDD144 pKa = 3.22KK145 pKa = 10.61FVQVFNFEE153 pKa = 4.3LSVVLGAAA161 pKa = 3.65

Molecular weight: 18.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10394

56

1195

199.9

22.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.168 ± 0.58

1.068 ± 0.18

6.003 ± 0.297

6.783 ± 0.352

3.666 ± 0.182

6.369 ± 0.258

1.597 ± 0.169

6.157 ± 0.319

7.524 ± 0.386

9.188 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.19

5.08 ± 0.332

3.55 ± 0.269

4.724 ± 0.374

4.705 ± 0.298

6.34 ± 0.335

5.926 ± 0.357

5.792 ± 0.177

1.26 ± 0.127

3.589 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski