Mycobacterium virus Brujita

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Chebruvirinae; Brujitavirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5U392|B5U392_9CAUD Uncharacterized protein OS=Mycobacterium virus Brujita OX=561996 GN=24 PE=4 SV=1
MM1 pKa = 7.65ANEE4 pKa = 5.45LYY6 pKa = 10.21TKK8 pKa = 10.41YY9 pKa = 10.27KK10 pKa = 10.56EE11 pKa = 4.03KK12 pKa = 10.95ALDD15 pKa = 4.27GDD17 pKa = 4.31ISWTGDD23 pKa = 3.4NIRR26 pKa = 11.84VYY28 pKa = 10.85LVDD31 pKa = 4.14ANDD34 pKa = 3.5YY35 pKa = 9.39TVDD38 pKa = 3.87LDD40 pKa = 4.16ADD42 pKa = 4.05EE43 pKa = 5.27FLDD46 pKa = 5.63DD47 pKa = 4.4IPSGAIVATSGNLASKK63 pKa = 9.18TVTGGVADD71 pKa = 3.97AANVTFSAVSGDD83 pKa = 3.0ISEE86 pKa = 4.75ALVIAKK92 pKa = 7.65YY93 pKa = 9.1TGTASTSPLIAYY105 pKa = 9.39IDD107 pKa = 4.27DD108 pKa = 3.83ATGLAVTPNGGDD120 pKa = 3.18ITIIWPDD127 pKa = 3.41DD128 pKa = 4.15GIFEE132 pKa = 4.58LL133 pKa = 5.36

Molecular weight: 13.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5U3A2|B5U3A2_9CAUD Uncharacterized protein OS=Mycobacterium virus Brujita OX=561996 GN=34 PE=4 SV=1
MM1 pKa = 7.26TRR3 pKa = 11.84EE4 pKa = 4.04QTDD7 pKa = 3.3SPRR10 pKa = 11.84LVSSGFKK17 pKa = 10.16GQYY20 pKa = 9.49PMVNRR25 pKa = 11.84WRR27 pKa = 11.84TWQFAQSLSARR38 pKa = 11.84TVEE41 pKa = 4.0EE42 pKa = 3.63RR43 pKa = 11.84VATVRR48 pKa = 11.84RR49 pKa = 11.84MAAWCGVEE57 pKa = 4.34PEE59 pKa = 4.35FAQVEE64 pKa = 4.58QIVSWLAEE72 pKa = 4.04GGNWSARR79 pKa = 11.84TRR81 pKa = 11.84WTYY84 pKa = 11.34YY85 pKa = 10.31GALSAWFLWLQQQGHH100 pKa = 5.14RR101 pKa = 11.84HH102 pKa = 6.2DD103 pKa = 4.56NPMVMIGRR111 pKa = 11.84PKK113 pKa = 10.09RR114 pKa = 11.84PKK116 pKa = 9.75SVPRR120 pKa = 11.84PVSNLDD126 pKa = 3.29VQRR129 pKa = 11.84LLAVRR134 pKa = 11.84AHH136 pKa = 6.47KK137 pKa = 9.25RR138 pKa = 11.84TKK140 pKa = 11.33AMILLAAFQGLRR152 pKa = 11.84VHH154 pKa = 7.38EE155 pKa = 4.11IAQIKK160 pKa = 10.05GEE162 pKa = 4.07HH163 pKa = 6.8LDD165 pKa = 4.12LIEE168 pKa = 4.13RR169 pKa = 11.84TMTVTGKK176 pKa = 11.13GNVTATLPLHH186 pKa = 5.96HH187 pKa = 6.79RR188 pKa = 11.84VVEE191 pKa = 4.15IAYY194 pKa = 8.29QMPRR198 pKa = 11.84RR199 pKa = 11.84GHH201 pKa = 6.73WFPGPDD207 pKa = 2.81RR208 pKa = 11.84GHH210 pKa = 5.51QRR212 pKa = 11.84RR213 pKa = 11.84EE214 pKa = 4.31SVSGTIKK221 pKa = 10.28EE222 pKa = 4.01AMIRR226 pKa = 11.84AGVVGSAHH234 pKa = 7.08CLRR237 pKa = 11.84HH238 pKa = 4.94WFGTALLEE246 pKa = 4.69AGVDD250 pKa = 3.56LRR252 pKa = 11.84TVQEE256 pKa = 4.11LMRR259 pKa = 11.84HH260 pKa = 4.7QSLTSTEE267 pKa = 3.62IYY269 pKa = 10.3TRR271 pKa = 11.84VTDD274 pKa = 3.31QRR276 pKa = 11.84RR277 pKa = 11.84AEE279 pKa = 4.59GIEE282 pKa = 3.77RR283 pKa = 11.84LDD285 pKa = 3.76PFRR288 pKa = 11.84VAPATRR294 pKa = 11.84VTDD297 pKa = 3.71RR298 pKa = 11.84LLAQIAEE305 pKa = 4.29RR306 pKa = 11.84DD307 pKa = 3.57EE308 pKa = 4.58GAPGAPLSAAA318 pKa = 3.87

Molecular weight: 36.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

15101

39

1276

204.1

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.39 ± 0.52

0.887 ± 0.115

6.88 ± 0.257

5.986 ± 0.322

2.881 ± 0.165

9.033 ± 0.511

1.94 ± 0.167

4.477 ± 0.205

3.278 ± 0.199

7.847 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.13

3.271 ± 0.191

6.072 ± 0.207

3.649 ± 0.205

7.377 ± 0.462

5.145 ± 0.189

5.9 ± 0.224

7.145 ± 0.273

2.073 ± 0.136

2.616 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski