Avon-Heathcote Estuary associated circular virus 10

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IBL9|A0A0C5IBL9_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 10 OX=1618233 PE=3 SV=1
MM1 pKa = 7.84PVTQPVTKK9 pKa = 9.21ATRR12 pKa = 11.84AICFTEE18 pKa = 4.97FKK20 pKa = 10.93KK21 pKa = 9.75LTNWDD26 pKa = 3.68DD27 pKa = 3.5VKK29 pKa = 11.44SQLRR33 pKa = 11.84YY34 pKa = 8.63YY35 pKa = 10.81AYY37 pKa = 10.37AEE39 pKa = 4.19EE40 pKa = 4.53TCPKK44 pKa = 9.18TGKK47 pKa = 9.49KK48 pKa = 9.54HH49 pKa = 4.62YY50 pKa = 10.29QGFAYY55 pKa = 9.52SWKK58 pKa = 9.91PIRR61 pKa = 11.84FSGWKK66 pKa = 10.13KK67 pKa = 9.21MFPTAHH73 pKa = 6.45IEE75 pKa = 3.86QMRR78 pKa = 11.84GNFRR82 pKa = 11.84EE83 pKa = 3.94NTAYY87 pKa = 10.38CSKK90 pKa = 10.12EE91 pKa = 3.41GSLIEE96 pKa = 4.74FGEE99 pKa = 4.3KK100 pKa = 9.98PNEE103 pKa = 4.0NGVQGTAIEE112 pKa = 4.1YY113 pKa = 9.97KK114 pKa = 10.28RR115 pKa = 11.84RR116 pKa = 11.84IEE118 pKa = 4.21EE119 pKa = 4.25GQSTMEE125 pKa = 3.87IAEE128 pKa = 4.43DD129 pKa = 3.72EE130 pKa = 4.44NLFGQYY136 pKa = 9.63LHH138 pKa = 7.13SYY140 pKa = 9.33SALEE144 pKa = 4.45KK145 pKa = 10.44YY146 pKa = 8.01EE147 pKa = 3.59QHH149 pKa = 6.7IRR151 pKa = 11.84GKK153 pKa = 10.2KK154 pKa = 8.87IKK156 pKa = 10.34LDD158 pKa = 3.52EE159 pKa = 4.65TPPEE163 pKa = 4.15VYY165 pKa = 10.07IRR167 pKa = 11.84WGAAGTGKK175 pKa = 7.89THH177 pKa = 6.96GVFKK181 pKa = 10.72RR182 pKa = 11.84YY183 pKa = 9.84GRR185 pKa = 11.84DD186 pKa = 3.14KK187 pKa = 11.36VCIVPDD193 pKa = 3.67NTGKK197 pKa = 9.76WFDD200 pKa = 3.51NCHH203 pKa = 6.2NSDD206 pKa = 3.24VMLFDD211 pKa = 5.0DD212 pKa = 4.75VEE214 pKa = 4.77IDD216 pKa = 3.53AVPPISQFLRR226 pKa = 11.84LTDD229 pKa = 4.52RR230 pKa = 11.84YY231 pKa = 10.06PMQVPVKK238 pKa = 10.65GGFIWWKK245 pKa = 8.36PKK247 pKa = 10.8VIVFTSNLPWEE258 pKa = 4.51QWWNTLNTEE267 pKa = 4.24HH268 pKa = 6.84KK269 pKa = 10.13RR270 pKa = 11.84AVQRR274 pKa = 11.84RR275 pKa = 11.84LTRR278 pKa = 11.84VTRR281 pKa = 11.84IYY283 pKa = 10.59KK284 pKa = 9.71DD285 pKa = 3.39HH286 pKa = 7.06EE287 pKa = 4.02KK288 pKa = 10.96VEE290 pKa = 4.34YY291 pKa = 10.74EE292 pKa = 3.89NANYY296 pKa = 10.0EE297 pKa = 4.71LLSQTQGFFASPPQLQQEE315 pKa = 4.53VQEE318 pKa = 4.71EE319 pKa = 4.4PVHH322 pKa = 5.7SQEE325 pKa = 4.5EE326 pKa = 5.08GEE328 pKa = 4.39QVHH331 pKa = 6.12EE332 pKa = 4.37EE333 pKa = 4.03SGSASEE339 pKa = 4.11EE340 pKa = 4.03HH341 pKa = 6.71SAVV344 pKa = 3.35

Molecular weight: 40.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IBL9|A0A0C5IBL9_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 10 OX=1618233 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 10.09KK3 pKa = 9.55WNTKK7 pKa = 8.58MPITSYY13 pKa = 9.85FRR15 pKa = 11.84KK16 pKa = 9.88RR17 pKa = 11.84RR18 pKa = 11.84ASSQARR24 pKa = 11.84PNYY27 pKa = 9.77SKK29 pKa = 10.9KK30 pKa = 10.41SKK32 pKa = 10.13KK33 pKa = 10.0NPYY36 pKa = 6.4TRR38 pKa = 11.84KK39 pKa = 9.87RR40 pKa = 11.84KK41 pKa = 8.94ASKK44 pKa = 9.18YY45 pKa = 6.8MKK47 pKa = 10.13RR48 pKa = 11.84AVAQAKK54 pKa = 8.4STARR58 pKa = 11.84FNTQVKK64 pKa = 10.05NVMLAMEE71 pKa = 4.16EE72 pKa = 4.32TKK74 pKa = 11.08YY75 pKa = 9.66KK76 pKa = 7.95TTNLTGLPAYY86 pKa = 7.6ATTMYY91 pKa = 10.35HH92 pKa = 6.78NKK94 pKa = 8.49LTSFKK99 pKa = 10.56LWEE102 pKa = 4.12QGSNGTMALFPLQRR116 pKa = 11.84GTDD119 pKa = 3.16QDD121 pKa = 4.02EE122 pKa = 4.35IVGRR126 pKa = 11.84EE127 pKa = 3.99YY128 pKa = 10.86YY129 pKa = 10.77AKK131 pKa = 10.25SLKK134 pKa = 9.72IDD136 pKa = 3.55LQFTFPYY143 pKa = 10.26DD144 pKa = 3.51RR145 pKa = 11.84LGSTIKK151 pKa = 10.14VWYY154 pKa = 9.9VPVKK158 pKa = 9.89TGQPEE163 pKa = 3.64PAYY166 pKa = 10.71DD167 pKa = 3.37EE168 pKa = 4.77FFRR171 pKa = 11.84NTSGNIMLDD180 pKa = 3.27PRR182 pKa = 11.84NMQNYY187 pKa = 8.58PYY189 pKa = 10.6AKK191 pKa = 9.13YY192 pKa = 10.28LGSFRR197 pKa = 11.84PRR199 pKa = 11.84VKK201 pKa = 9.45QTTFGTLVNGNGVLGGRR218 pKa = 11.84DD219 pKa = 3.23ATVVKK224 pKa = 10.17SYY226 pKa = 10.61RR227 pKa = 11.84ISFNKK232 pKa = 9.41FFKK235 pKa = 10.62LRR237 pKa = 11.84SNVGGDD243 pKa = 3.64AEE245 pKa = 5.06IDD247 pKa = 3.86FNDD250 pKa = 3.94IPNLAEE256 pKa = 3.84KK257 pKa = 10.77AFLVFTAYY265 pKa = 10.63NEE267 pKa = 4.03EE268 pKa = 4.15AALEE272 pKa = 4.04TDD274 pKa = 3.54QVISNVEE281 pKa = 3.84GAFTFSYY288 pKa = 10.84KK289 pKa = 10.81DD290 pKa = 3.24PP291 pKa = 4.43

Molecular weight: 33.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

635

291

344

317.5

36.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.299 ± 0.8

0.787 ± 0.5

3.937 ± 0.118

7.717 ± 2.061

5.354 ± 0.527

6.142 ± 0.19

1.89 ± 0.981

3.937 ± 0.536

8.819 ± 0.728

5.354 ± 0.527

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.464

5.354 ± 0.963

5.039 ± 0.145

4.882 ± 0.699

5.669 ± 0.328

5.669 ± 0.328

7.402 ± 0.755

5.984 ± 0.308

2.047 ± 0.645

5.354 ± 0.527
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski