Flavobacterium sp. BFFFF2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257K9L4|A0A257K9L4_9FLAO Uncharacterized protein OS=Flavobacterium sp. BFFFF2 OX=2015558 GN=CFE24_09210 PE=4 SV=1
MM1 pKa = 7.86IYY3 pKa = 10.37NIRR6 pKa = 11.84AILDD10 pKa = 3.49TEE12 pKa = 3.94EE13 pKa = 5.04DD14 pKa = 3.87VFRR17 pKa = 11.84DD18 pKa = 3.21IAIRR22 pKa = 11.84DD23 pKa = 3.65TDD25 pKa = 3.8TLEE28 pKa = 5.46DD29 pKa = 3.65LHH31 pKa = 8.21QSLINAFGFDD41 pKa = 3.97GMEE44 pKa = 3.66MASFYY49 pKa = 10.78TCDD52 pKa = 4.12DD53 pKa = 3.05RR54 pKa = 11.84WNQEE58 pKa = 3.73DD59 pKa = 5.14EE60 pKa = 4.39IPLFDD65 pKa = 4.22TGDD68 pKa = 3.52EE69 pKa = 4.27PGQVQTMNDD78 pKa = 3.4YY79 pKa = 11.39SLLEE83 pKa = 4.39LLHH86 pKa = 6.82EE87 pKa = 4.56DD88 pKa = 3.23HH89 pKa = 6.89TKK91 pKa = 10.22IIYY94 pKa = 10.36VYY96 pKa = 10.7DD97 pKa = 4.28FLNMWTFLVEE107 pKa = 3.98LASITEE113 pKa = 4.08EE114 pKa = 4.03QVGVVYY120 pKa = 10.46PEE122 pKa = 4.34LMHH125 pKa = 6.55AHH127 pKa = 6.23GVLPEE132 pKa = 4.17SAPNAQFEE140 pKa = 4.9SDD142 pKa = 3.25GTEE145 pKa = 3.85NNPYY149 pKa = 10.96ADD151 pKa = 4.81FEE153 pKa = 5.26DD154 pKa = 6.22DD155 pKa = 3.65YY156 pKa = 12.06DD157 pKa = 5.03EE158 pKa = 6.87DD159 pKa = 6.18DD160 pKa = 5.16FDD162 pKa = 5.44QEE164 pKa = 4.72DD165 pKa = 4.13EE166 pKa = 4.66FGGEE170 pKa = 4.07HH171 pKa = 6.59YY172 pKa = 10.5EE173 pKa = 4.75DD174 pKa = 5.79LGFEE178 pKa = 4.84DD179 pKa = 3.59NWNN182 pKa = 3.42

Molecular weight: 21.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257KC55|A0A257KC55_9FLAO ATPase OS=Flavobacterium sp. BFFFF2 OX=2015558 GN=CFE24_04360 PE=4 SV=1
MM1 pKa = 7.64LSSLKK6 pKa = 10.44KK7 pKa = 9.14ATVKK11 pKa = 10.22TFMATVKK18 pKa = 10.04TFMASGKK25 pKa = 8.24TFMASGKK32 pKa = 8.27TFMATVKK39 pKa = 10.01TFMTSVQTFMTAFKK53 pKa = 9.97SLPIAMVIGRR63 pKa = 11.84RR64 pKa = 11.84GLGVRR69 pKa = 11.84RR70 pKa = 11.84PRR72 pKa = 11.84HH73 pKa = 4.21QSKK76 pKa = 10.82

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3021

0

3021

1062431

38

9436

351.7

39.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.347 ± 0.086

1.03 ± 0.024

4.95 ± 0.057

5.396 ± 0.078

5.171 ± 0.046

6.538 ± 0.067

1.942 ± 0.032

7.011 ± 0.039

6.374 ± 0.087

9.279 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.172 ± 0.032

5.818 ± 0.05

3.779 ± 0.033

4.323 ± 0.04

3.461 ± 0.049

6.833 ± 0.084

6.965 ± 0.171

6.394 ± 0.048

1.246 ± 0.022

3.972 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski