Arthrobacter phage Makai

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordonvirus; unclassified Gordonvirus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D8S9|A0A5P8D8S9_9CAUD Uncharacterized protein OS=Arthrobacter phage Makai OX=2652409 GN=35 PE=4 SV=1
MM1 pKa = 7.53TKK3 pKa = 10.5LIWGSAGKK11 pKa = 9.71QFYY14 pKa = 9.9EE15 pKa = 4.19AGTDD19 pKa = 3.25RR20 pKa = 11.84GVLYY24 pKa = 11.02LDD26 pKa = 3.56GTGVAWEE33 pKa = 4.46GLVSVDD39 pKa = 3.82EE40 pKa = 4.53SPSGGEE46 pKa = 4.0SEE48 pKa = 4.31KK49 pKa = 10.93YY50 pKa = 10.56YY51 pKa = 10.97YY52 pKa = 10.63DD53 pKa = 3.81GLPYY57 pKa = 10.1IHH59 pKa = 6.95TSSVEE64 pKa = 3.81EE65 pKa = 4.07FKK67 pKa = 11.27ASIEE71 pKa = 4.19AYY73 pKa = 6.65TWPVEE78 pKa = 4.11FAQCDD83 pKa = 3.69GSEE86 pKa = 4.48EE87 pKa = 4.16IAQGLYY93 pKa = 9.99ISQQNRR99 pKa = 11.84KK100 pKa = 8.73EE101 pKa = 3.92FGLSYY106 pKa = 9.69RR107 pKa = 11.84TLVGNDD113 pKa = 3.28LDD115 pKa = 4.36EE116 pKa = 5.64LEE118 pKa = 4.58HH119 pKa = 6.82AYY121 pKa = 9.73KK122 pKa = 10.31IHH124 pKa = 7.0IVYY127 pKa = 10.63NCMASSSSRR136 pKa = 11.84AYY138 pKa = 9.66STMGDD143 pKa = 3.6GVDD146 pKa = 3.19PTMFKK151 pKa = 10.26WDD153 pKa = 3.07ISTRR157 pKa = 11.84PVKK160 pKa = 10.8FEE162 pKa = 4.49DD163 pKa = 3.62PAFGTRR169 pKa = 11.84YY170 pKa = 9.45GAHH173 pKa = 6.65LVLDD177 pKa = 4.06SRR179 pKa = 11.84EE180 pKa = 4.06MYY182 pKa = 9.79PWALTAVEE190 pKa = 4.22DD191 pKa = 3.97VLYY194 pKa = 8.81GTDD197 pKa = 3.37TEE199 pKa = 4.58APRR202 pKa = 11.84LPTPQEE208 pKa = 3.79LLALFIDD215 pKa = 3.83NAILKK220 pKa = 8.61ITDD223 pKa = 3.69NGDD226 pKa = 3.63GTWTADD232 pKa = 3.66GPDD235 pKa = 4.32SIISMLSTDD244 pKa = 3.36VFQINWTSANMIDD257 pKa = 3.58EE258 pKa = 4.83DD259 pKa = 4.84KK260 pKa = 10.64YY261 pKa = 9.35TVSSFF266 pKa = 3.16

Molecular weight: 29.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D907|A0A5P8D907_9CAUD Uncharacterized protein OS=Arthrobacter phage Makai OX=2652409 GN=77 PE=4 SV=1
MM1 pKa = 7.43GAGLKK6 pKa = 10.12PVFKK10 pKa = 9.48PACPKK15 pKa = 10.64CPFEE19 pKa = 4.59LKK21 pKa = 10.34YY22 pKa = 10.41HH23 pKa = 5.22VRR25 pKa = 11.84DD26 pKa = 4.13PEE28 pKa = 4.07VHH30 pKa = 6.42PDD32 pKa = 2.93GWVLTEE38 pKa = 4.33ASRR41 pKa = 11.84KK42 pKa = 9.28HH43 pKa = 5.66LAEE46 pKa = 3.81AHH48 pKa = 6.03IEE50 pKa = 4.19VPKK53 pKa = 10.21VRR55 pKa = 11.84KK56 pKa = 6.51VTKK59 pKa = 9.09PVRR62 pKa = 11.84RR63 pKa = 11.84HH64 pKa = 4.25SRR66 pKa = 11.84RR67 pKa = 11.84TTVLRR72 pKa = 11.84LPSSRR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84ALVV82 pKa = 2.98

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

18120

41

1530

197.0

21.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.83 ± 0.451

0.508 ± 0.086

5.977 ± 0.287

6.54 ± 0.335

3.659 ± 0.204

7.577 ± 0.473

1.821 ± 0.168

6.214 ± 0.217

6.915 ± 0.344

7.958 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.853 ± 0.153

4.564 ± 0.19

4.062 ± 0.227

3.4 ± 0.147

5.204 ± 0.302

6.087 ± 0.195

6.115 ± 0.295

6.827 ± 0.176

1.507 ± 0.122

3.383 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski