Blautia sp. CAG:52

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia; environmental samples

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2465 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6G8M4|R6G8M4_9FIRM Uncharacterized protein OS=Blautia sp. CAG:52 OX=1262758 GN=BN690_00824 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 9.78KK3 pKa = 10.03KK4 pKa = 9.68WMASTALACSCMLLISGCSGNKK26 pKa = 9.71ADD28 pKa = 5.92DD29 pKa = 3.98FDD31 pKa = 4.89AVAYY35 pKa = 5.98TTAYY39 pKa = 9.51LDD41 pKa = 3.33GMIRR45 pKa = 11.84GNVDD49 pKa = 3.33TLSSLSGVSSDD60 pKa = 5.38DD61 pKa = 3.2LTATFNSMIDD71 pKa = 3.78DD72 pKa = 4.53LVSSSIGGDD81 pKa = 3.29GSSPGSTQISPQLRR95 pKa = 11.84QDD97 pKa = 3.4YY98 pKa = 10.65TDD100 pKa = 3.55FWKK103 pKa = 10.7QAFSNTKK110 pKa = 9.92YY111 pKa = 10.38DD112 pKa = 3.49VKK114 pKa = 10.98KK115 pKa = 10.73ADD117 pKa = 3.79KK118 pKa = 10.89KK119 pKa = 10.43EE120 pKa = 3.68NTYY123 pKa = 10.81QITVDD128 pKa = 3.63TQQMQLYY135 pKa = 7.99TAMEE139 pKa = 5.12PIYY142 pKa = 10.26DD143 pKa = 4.11EE144 pKa = 5.26KK145 pKa = 11.22LADD148 pKa = 3.76YY149 pKa = 11.0LKK151 pKa = 10.98DD152 pKa = 3.51NSVDD156 pKa = 3.24SDD158 pKa = 3.72QYY160 pKa = 11.38IEE162 pKa = 4.83DD163 pKa = 4.15VYY165 pKa = 11.83SLMLEE170 pKa = 4.83AYY172 pKa = 7.75QTALDD177 pKa = 3.64KK178 pKa = 11.58AEE180 pKa = 4.21YY181 pKa = 9.93NDD183 pKa = 4.25PEE185 pKa = 4.73SVTVTLQKK193 pKa = 10.79DD194 pKa = 3.91DD195 pKa = 4.06SEE197 pKa = 4.31QWSISSDD204 pKa = 4.06DD205 pKa = 3.42MTTLKK210 pKa = 10.77NALIDD215 pKa = 3.97LASLNPSADD224 pKa = 3.36GTTGTPAQVTAEE236 pKa = 4.96DD237 pKa = 3.71IANSQSEE244 pKa = 4.32GAPNMEE250 pKa = 4.07YY251 pKa = 10.15PKK253 pKa = 10.68DD254 pKa = 3.93LEE256 pKa = 4.14STPAYY261 pKa = 9.69KK262 pKa = 10.66VGDD265 pKa = 4.73AITIQSDD272 pKa = 3.69GKK274 pKa = 10.58DD275 pKa = 3.28VATFTIDD282 pKa = 3.18KK283 pKa = 11.21VEE285 pKa = 4.18VTDD288 pKa = 5.81DD289 pKa = 3.26RR290 pKa = 11.84SEE292 pKa = 4.4YY293 pKa = 9.84DD294 pKa = 2.97TSNPDD299 pKa = 3.01KK300 pKa = 11.07VIVITYY306 pKa = 7.23TYY308 pKa = 10.76KK309 pKa = 10.86NLAFDD314 pKa = 5.53DD315 pKa = 4.48PLLYY319 pKa = 10.74DD320 pKa = 3.24QMSFRR325 pKa = 11.84VLDD328 pKa = 3.94GDD330 pKa = 4.56TVCLPYY336 pKa = 10.9YY337 pKa = 10.44LADD340 pKa = 3.69LTSPDD345 pKa = 3.85LATTGGDD352 pKa = 3.23AVTATAAYY360 pKa = 9.22GISASCNEE368 pKa = 3.47VTIYY372 pKa = 11.17VDD374 pKa = 4.01GAQIAFPFQVSASVSS389 pKa = 3.32

Molecular weight: 42.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6G8F1|R6G8F1_9FIRM HTH lysR-type domain-containing protein OS=Blautia sp. CAG:52 OX=1262758 GN=BN690_00792 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.56GRR39 pKa = 11.84KK40 pKa = 8.92SLSAA44 pKa = 3.86

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2465

0

2465

785732

29

2004

318.8

35.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.318 ± 0.048

1.556 ± 0.019

5.498 ± 0.04

7.89 ± 0.059

3.911 ± 0.037

7.355 ± 0.044

1.829 ± 0.02

7.044 ± 0.047

6.761 ± 0.045

8.879 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.227 ± 0.027

3.999 ± 0.029

3.406 ± 0.026

3.642 ± 0.036

4.425 ± 0.038

5.421 ± 0.037

5.577 ± 0.039

7.05 ± 0.041

1.057 ± 0.021

4.156 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski