Amino acid dipepetide frequency for Streptomyces sp. Root264

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.754AlaAla: 21.754 ± 0.169	1.108AlaCys: 1.108 ± 0.025	8.595AlaAsp: 8.595 ± 0.073	8.683AlaGlu: 8.683 ± 0.074	3.585AlaPhe: 3.585 ± 0.043	13.374AlaGly: 13.374 ± 0.09	2.994AlaHis: 2.994 ± 0.039	3.212AlaIle: 3.212 ± 0.048	2.903AlaLys: 2.903 ± 0.048	14.746AlaLeu: 14.746 ± 0.112	2.41AlaMet: 2.41 ± 0.034	1.992AlaAsn: 1.992 ± 0.035	7.166AlaPro: 7.166 ± 0.074	3.927AlaGln: 3.927 ± 0.048	10.474AlaArg: 10.474 ± 0.082	6.187AlaSer: 6.187 ± 0.058	7.048AlaThr: 7.048 ± 0.058	12.699AlaVal: 12.699 ± 0.092	1.892AlaTrp: 1.892 ± 0.03	2.856AlaTyr: 2.856 ± 0.033	0.005AlaXaa: 0.005 ± 0.001
Cys	1.138CysAla: 1.138 ± 0.027	0.097CysCys: 0.097 ± 0.007	0.474CysAsp: 0.474 ± 0.014	0.399CysGlu: 0.399 ± 0.014	0.235CysPhe: 0.235 ± 0.01	0.987CysGly: 0.987 ± 0.019	0.19CysHis: 0.19 ± 0.01	0.172CysIle: 0.172 ± 0.009	0.114CysLys: 0.114 ± 0.007	0.767CysLeu: 0.767 ± 0.019	0.125CysMet: 0.125 ± 0.006	0.139CysAsn: 0.139 ± 0.008	0.48CysPro: 0.48 ± 0.017	0.149CysGln: 0.149 ± 0.008	0.6CysArg: 0.6 ± 0.018	0.433CysSer: 0.433 ± 0.014	0.531CysThr: 0.531 ± 0.015	0.692CysVal: 0.692 ± 0.019	0.139CysTrp: 0.139 ± 0.007	0.138CysTyr: 0.138 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.019AspAla: 8.019 ± 0.071	0.453AspCys: 0.453 ± 0.016	3.809AspAsp: 3.809 ± 0.047	3.845AspGlu: 3.845 ± 0.044	1.709AspPhe: 1.709 ± 0.029	6.485AspGly: 6.485 ± 0.063	1.431AspHis: 1.431 ± 0.024	1.741AspIle: 1.741 ± 0.026	1.145AspLys: 1.145 ± 0.028	6.231AspLeu: 6.231 ± 0.055	0.836AspMet: 0.836 ± 0.021	0.992AspAsn: 0.992 ± 0.021	4.438AspPro: 4.438 ± 0.052	1.458AspGln: 1.458 ± 0.026	4.942AspArg: 4.942 ± 0.048	2.537AspSer: 2.537 ± 0.033	3.153AspThr: 3.153 ± 0.04	5.108AspVal: 5.108 ± 0.052	1.057AspTrp: 1.057 ± 0.024	1.095AspTyr: 1.095 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.346GluAla: 7.346 ± 0.072	0.362GluCys: 0.362 ± 0.015	2.732GluAsp: 2.732 ± 0.033	3.503GluGlu: 3.503 ± 0.043	1.455GluPhe: 1.455 ± 0.029	4.25GluGly: 4.25 ± 0.046	1.509GluHis: 1.509 ± 0.026	2.214GluIle: 2.214 ± 0.039	1.378GluLys: 1.378 ± 0.029	6.589GluLeu: 6.589 ± 0.057	0.874GluMet: 0.874 ± 0.021	1.051GluAsn: 1.051 ± 0.023	3.34GluPro: 3.34 ± 0.048	2.253GluGln: 2.253 ± 0.036	5.343GluArg: 5.343 ± 0.059	2.549GluSer: 2.549 ± 0.035	2.837GluThr: 2.837 ± 0.038	4.357GluVal: 4.357 ± 0.046	0.749GluTrp: 0.749 ± 0.019	1.09GluTyr: 1.09 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.762PheAla: 3.762 ± 0.042	0.272PheCys: 0.272 ± 0.012	1.953PheAsp: 1.953 ± 0.03	1.417PheGlu: 1.417 ± 0.024	0.867PhePhe: 0.867 ± 0.023	2.984PheGly: 2.984 ± 0.036	0.629PheHis: 0.629 ± 0.015	0.66PheIle: 0.66 ± 0.02	0.51PheLys: 0.51 ± 0.014	2.572PheLeu: 2.572 ± 0.038	0.401PheMet: 0.401 ± 0.013	0.558PheAsn: 0.558 ± 0.017	1.357PhePro: 1.357 ± 0.023	0.706PheGln: 0.706 ± 0.017	1.883PheArg: 1.883 ± 0.027	1.49PheSer: 1.49 ± 0.027	2.002PheThr: 2.002 ± 0.032	2.322PheVal: 2.322 ± 0.034	0.418PheTrp: 0.418 ± 0.016	0.576PheTyr: 0.576 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.541GlyAla: 11.541 ± 0.089	0.82GlyCys: 0.82 ± 0.021	5.356GlyAsp: 5.356 ± 0.053	5.023GlyGlu: 5.023 ± 0.044	2.885GlyPhe: 2.885 ± 0.037	8.984GlyGly: 8.984 ± 0.106	2.306GlyHis: 2.306 ± 0.034	3.169GlyIle: 3.169 ± 0.041	2.369GlyLys: 2.369 ± 0.039	9.402GlyLeu: 9.402 ± 0.069	1.915GlyMet: 1.915 ± 0.029	1.784GlyAsn: 1.784 ± 0.041	5.233GlyPro: 5.233 ± 0.06	2.65GlyGln: 2.65 ± 0.034	7.996GlyArg: 7.996 ± 0.065	5.531GlySer: 5.531 ± 0.052	5.991GlyThr: 5.991 ± 0.062	7.917GlyVal: 7.917 ± 0.068	1.716GlyTrp: 1.716 ± 0.029	2.291GlyTyr: 2.291 ± 0.042	0.003GlyXaa: 0.003 ± 0.001
His	2.792HisAla: 2.792 ± 0.04	0.207HisCys: 0.207 ± 0.009	1.421HisAsp: 1.421 ± 0.023	1.226HisGlu: 1.226 ± 0.025	0.617HisPhe: 0.617 ± 0.017	2.517HisGly: 2.517 ± 0.034	0.688HisHis: 0.688 ± 0.019	0.637HisIle: 0.637 ± 0.018	0.356HisLys: 0.356 ± 0.012	2.384HisLeu: 2.384 ± 0.027	0.328HisMet: 0.328 ± 0.012	0.376HisAsn: 0.376 ± 0.012	1.797HisPro: 1.797 ± 0.03	0.618HisGln: 0.618 ± 0.017	2.113HisArg: 2.113 ± 0.032	1.023HisSer: 1.023 ± 0.023	1.397HisThr: 1.397 ± 0.028	1.754HisVal: 1.754 ± 0.031	0.382HisTrp: 0.382 ± 0.015	0.486HisTyr: 0.486 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.609IleAla: 4.609 ± 0.056	0.269IleCys: 0.269 ± 0.011	2.144IleAsp: 2.144 ± 0.033	1.801IleGlu: 1.801 ± 0.032	0.623IlePhe: 0.623 ± 0.018	3.307IleGly: 3.307 ± 0.049	0.558IleHis: 0.558 ± 0.017	0.788IleIle: 0.788 ± 0.024	0.687IleLys: 0.687 ± 0.019	2.307IleLeu: 2.307 ± 0.034	0.434IleMet: 0.434 ± 0.015	0.645IleAsn: 0.645 ± 0.019	1.655IlePro: 1.655 ± 0.029	0.668IleGln: 0.668 ± 0.019	2.154IleArg: 2.154 ± 0.03	1.579IleSer: 1.579 ± 0.029	2.125IleThr: 2.125 ± 0.035	2.712IleVal: 2.712 ± 0.035	0.336IleTrp: 0.336 ± 0.012	0.482IleTyr: 0.482 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.028LysAla: 3.028 ± 0.043	0.117LysCys: 0.117 ± 0.008	1.317LysAsp: 1.317 ± 0.029	1.119LysGlu: 1.119 ± 0.028	0.449LysPhe: 0.449 ± 0.016	1.822LysGly: 1.822 ± 0.033	0.412LysHis: 0.412 ± 0.014	0.861LysIle: 0.861 ± 0.023	0.845LysLys: 0.845 ± 0.026	2.0LysLeu: 2.0 ± 0.032	0.372LysMet: 0.372 ± 0.013	0.541LysAsn: 0.541 ± 0.019	1.296LysPro: 1.296 ± 0.029	0.682LysGln: 0.682 ± 0.019	1.332LysArg: 1.332 ± 0.025	1.118LysSer: 1.118 ± 0.024	1.314LysThr: 1.314 ± 0.03	1.922LysVal: 1.922 ± 0.035	0.274LysTrp: 0.274 ± 0.011	0.484LysTyr: 0.484 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.068LeuAla: 15.068 ± 0.115	0.863LeuCys: 0.863 ± 0.02	6.616LeuAsp: 6.616 ± 0.062	4.755LeuGlu: 4.755 ± 0.048	2.591LeuPhe: 2.591 ± 0.04	9.132LeuGly: 9.132 ± 0.073	2.405LeuHis: 2.405 ± 0.031	3.087LeuIle: 3.087 ± 0.038	2.041LeuLys: 2.041 ± 0.037	11.303LeuLeu: 11.303 ± 0.095	1.611LeuMet: 1.611 ± 0.028	1.681LeuAsn: 1.681 ± 0.03	6.433LeuPro: 6.433 ± 0.051	2.303LeuGln: 2.303 ± 0.033	8.661LeuArg: 8.661 ± 0.075	5.278LeuSer: 5.278 ± 0.046	6.958LeuThr: 6.958 ± 0.058	8.887LeuVal: 8.887 ± 0.07	1.314LeuTrp: 1.314 ± 0.027	1.904LeuTyr: 1.904 ± 0.031	0.001LeuXaa: 0.001 ± 0.001
Met	2.192MetAla: 2.192 ± 0.034	0.135MetCys: 0.135 ± 0.007	0.886MetAsp: 0.886 ± 0.021	0.721MetGlu: 0.721 ± 0.02	0.443MetPhe: 0.443 ± 0.015	1.272MetGly: 1.272 ± 0.026	0.361MetHis: 0.361 ± 0.013	0.639MetIle: 0.639 ± 0.017	0.417MetLys: 0.417 ± 0.015	1.597MetLeu: 1.597 ± 0.028	0.283MetMet: 0.283 ± 0.012	0.426MetAsn: 0.426 ± 0.013	1.091MetPro: 1.091 ± 0.022	0.46MetGln: 0.46 ± 0.013	1.398MetArg: 1.398 ± 0.023	1.342MetSer: 1.342 ± 0.021	1.547MetThr: 1.547 ± 0.024	1.246MetVal: 1.246 ± 0.024	0.211MetTrp: 0.211 ± 0.008	0.318MetTyr: 0.318 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.276AsnAla: 2.276 ± 0.033	0.179AsnCys: 0.179 ± 0.009	0.967AsnAsp: 0.967 ± 0.022	0.798AsnGlu: 0.798 ± 0.02	0.487AsnPhe: 0.487 ± 0.016	1.986AsnGly: 1.986 ± 0.034	0.389AsnHis: 0.389 ± 0.013	0.61AsnIle: 0.61 ± 0.019	0.411AsnLys: 0.411 ± 0.015	1.659AsnLeu: 1.659 ± 0.028	0.306AsnMet: 0.306 ± 0.013	0.452AsnAsn: 0.452 ± 0.019	1.349AsnPro: 1.349 ± 0.026	0.523AsnGln: 0.523 ± 0.016	1.265AsnArg: 1.265 ± 0.024	0.962AsnSer: 0.962 ± 0.023	1.1AsnThr: 1.1 ± 0.028	1.412AsnVal: 1.412 ± 0.026	0.298AsnTrp: 0.298 ± 0.013	0.409AsnTyr: 0.409 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	8.732ProAla: 8.732 ± 0.085	0.353ProCys: 0.353 ± 0.012	4.466ProAsp: 4.466 ± 0.05	4.156ProGlu: 4.156 ± 0.052	1.546ProPhe: 1.546 ± 0.024	6.635ProGly: 6.635 ± 0.066	1.411ProHis: 1.411 ± 0.028	1.142ProIle: 1.142 ± 0.021	1.12ProLys: 1.12 ± 0.026	5.352ProLeu: 5.352 ± 0.051	0.984ProMet: 0.984 ± 0.022	0.878ProAsn: 0.878 ± 0.018	3.438ProPro: 3.438 ± 0.057	1.868ProGln: 1.868 ± 0.037	3.93ProArg: 3.93 ± 0.044	3.268ProSer: 3.268 ± 0.041	3.202ProThr: 3.202 ± 0.043	5.416ProVal: 5.416 ± 0.052	0.896ProTrp: 0.896 ± 0.02	1.389ProTyr: 1.389 ± 0.028	0.003ProXaa: 0.003 ± 0.001
Gln	3.796GlnAla: 3.796 ± 0.047	0.17GlnCys: 0.17 ± 0.008	1.48GlnAsp: 1.48 ± 0.028	1.455GlnGlu: 1.455 ± 0.028	0.674GlnPhe: 0.674 ± 0.018	2.378GlnGly: 2.378 ± 0.035	0.636GlnHis: 0.636 ± 0.017	1.086GlnIle: 1.086 ± 0.023	0.643GlnLys: 0.643 ± 0.019	2.95GlnLeu: 2.95 ± 0.038	0.482GlnMet: 0.482 ± 0.013	0.536GlnAsn: 0.536 ± 0.019	1.682GlnPro: 1.682 ± 0.036	1.271GlnGln: 1.271 ± 0.035	2.317GlnArg: 2.317 ± 0.037	1.313GlnSer: 1.313 ± 0.027	1.456GlnThr: 1.456 ± 0.025	2.405GlnVal: 2.405 ± 0.036	0.501GlnTrp: 0.501 ± 0.015	0.604GlnTyr: 0.604 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.887ArgAla: 9.887 ± 0.084	0.587ArgCys: 0.587 ± 0.017	4.211ArgAsp: 4.211 ± 0.044	4.752ArgGlu: 4.752 ± 0.056	2.375ArgPhe: 2.375 ± 0.031	5.803ArgGly: 5.803 ± 0.055	2.129ArgHis: 2.129 ± 0.033	3.099ArgIle: 3.099 ± 0.034	1.57ArgLys: 1.57 ± 0.027	9.055ArgLeu: 9.055 ± 0.071	1.696ArgMet: 1.696 ± 0.028	1.314ArgAsn: 1.314 ± 0.026	5.01ArgPro: 5.01 ± 0.055	2.312ArgGln: 2.312 ± 0.031	7.841ArgArg: 7.841 ± 0.084	4.157ArgSer: 4.157 ± 0.039	5.466ArgThr: 5.466 ± 0.045	5.956ArgVal: 5.956 ± 0.052	1.381ArgTrp: 1.381 ± 0.025	1.795ArgTyr: 1.795 ± 0.031	0.002ArgXaa: 0.002 ± 0.001
Ser	7.029SerAla: 7.029 ± 0.066	0.442SerCys: 0.442 ± 0.015	2.815SerAsp: 2.815 ± 0.038	2.379SerGlu: 2.379 ± 0.034	1.537SerPhe: 1.537 ± 0.026	6.079SerGly: 6.079 ± 0.064	1.04SerHis: 1.04 ± 0.021	1.376SerIle: 1.376 ± 0.027	1.04SerLys: 1.04 ± 0.025	4.847SerLeu: 4.847 ± 0.045	1.049SerMet: 1.049 ± 0.021	0.871SerAsn: 0.871 ± 0.022	3.229SerPro: 3.229 ± 0.041	1.23SerGln: 1.23 ± 0.022	3.707SerArg: 3.707 ± 0.042	3.05SerSer: 3.05 ± 0.04	3.141SerThr: 3.141 ± 0.042	4.384SerVal: 4.384 ± 0.045	0.925SerTrp: 0.925 ± 0.024	1.266SerTyr: 1.266 ± 0.023	0.002SerXaa: 0.002 ± 0.001
Thr	9.219ThrAla: 9.219 ± 0.071	0.448ThrCys: 0.448 ± 0.014	3.611ThrAsp: 3.611 ± 0.046	3.05ThrGlu: 3.05 ± 0.04	1.595ThrPhe: 1.595 ± 0.027	6.741ThrGly: 6.741 ± 0.052	1.224ThrHis: 1.224 ± 0.024	1.588ThrIle: 1.588 ± 0.032	1.125ThrLys: 1.125 ± 0.026	5.745ThrLeu: 5.745 ± 0.061	0.922ThrMet: 0.922 ± 0.02	1.0ThrAsn: 1.0 ± 0.024	4.127ThrPro: 4.127 ± 0.042	1.325ThrGln: 1.325 ± 0.021	3.889ThrArg: 3.889 ± 0.044	3.204ThrSer: 3.204 ± 0.047	3.848ThrThr: 3.848 ± 0.051	6.281ThrVal: 6.281 ± 0.059	0.926ThrTrp: 0.926 ± 0.023	1.331ThrTyr: 1.331 ± 0.026	0.002ThrXaa: 0.002 ± 0.001
Val	10.869ValAla: 10.869 ± 0.07	0.804ValCys: 0.804 ± 0.019	5.236ValAsp: 5.236 ± 0.049	4.734ValGlu: 4.734 ± 0.057	2.537ValPhe: 2.537 ± 0.038	6.745ValGly: 6.745 ± 0.056	2.047ValHis: 2.047 ± 0.03	2.748ValIle: 2.748 ± 0.037	1.831ValLys: 1.831 ± 0.031	9.557ValLeu: 9.557 ± 0.08	1.429ValMet: 1.429 ± 0.029	1.708ValAsn: 1.708 ± 0.03	5.238ValPro: 5.238 ± 0.054	2.194ValGln: 2.194 ± 0.032	7.364ValArg: 7.364 ± 0.064	4.432ValSer: 4.432 ± 0.043	5.774ValThr: 5.774 ± 0.051	8.313ValVal: 8.313 ± 0.08	1.211ValTrp: 1.211 ± 0.023	1.655ValTyr: 1.655 ± 0.026	0.001ValXaa: 0.001 ± 0.001
Trp	1.696TrpAla: 1.696 ± 0.029	0.146TrpCys: 0.146 ± 0.009	0.837TrpAsp: 0.837 ± 0.017	0.739TrpGlu: 0.739 ± 0.019	0.491TrpPhe: 0.491 ± 0.014	1.086TrpGly: 1.086 ± 0.025	0.368TrpHis: 0.368 ± 0.013	0.558TrpIle: 0.558 ± 0.016	0.391TrpLys: 0.391 ± 0.014	1.748TrpLeu: 1.748 ± 0.032	0.282TrpMet: 0.282 ± 0.011	0.457TrpAsn: 0.457 ± 0.015	0.778TrpPro: 0.778 ± 0.021	0.633TrpGln: 0.633 ± 0.018	1.33TrpArg: 1.33 ± 0.023	0.987TrpSer: 0.987 ± 0.023	1.111TrpThr: 1.111 ± 0.025	0.962TrpVal: 0.962 ± 0.021	0.338TrpTrp: 0.338 ± 0.013	0.388TrpTyr: 0.388 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.855TyrAla: 2.855 ± 0.034	0.168TyrCys: 0.168 ± 0.008	1.562TyrAsp: 1.562 ± 0.027	1.227TyrGlu: 1.227 ± 0.022	0.639TyrPhe: 0.639 ± 0.015	2.309TyrGly: 2.309 ± 0.035	0.39TyrHis: 0.39 ± 0.014	0.493TyrIle: 0.493 ± 0.016	0.411TyrLys: 0.411 ± 0.015	2.078TyrLeu: 2.078 ± 0.029	0.246TyrMet: 0.246 ± 0.01	0.436TyrAsn: 0.436 ± 0.015	1.045TyrPro: 1.045 ± 0.024	0.604TyrGln: 0.604 ± 0.017	1.858TyrArg: 1.858 ± 0.031	0.995TyrSer: 0.995 ± 0.025	1.199TyrThr: 1.199 ± 0.03	1.68TyrVal: 1.68 ± 0.03	0.362TyrTrp: 0.362 ± 0.013	0.482TyrTyr: 0.482 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.003XaaArg: 0.003 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.013XaaXaa: 0.013 ± 0.003

Statistics based on 7235 proteins (2342576 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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