Torque teno mini virus 2
Average proteome isoelectric point is 7.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9JG54|Q9JG54_9VIRU Uncharacterized protein OS=Torque teno mini virus 2 OX=687370 PE=4 SV=1
MM1 pKa = 7.22 SLWKK5 pKa = 7.89 PTKK8 pKa = 10.01 YY9 pKa = 10.42 GPKK12 pKa = 9.76 RR13 pKa = 11.84 LEE15 pKa = 4.0 LQWLNGCITSHH26 pKa = 7.63 DD27 pKa = 4.63 YY28 pKa = 10.88 ICGCDD33 pKa = 3.66 DD34 pKa = 3.83 PAKK37 pKa = 10.52 HH38 pKa = 5.62 FLKK41 pKa = 10.98 VLVEE45 pKa = 4.48 KK46 pKa = 10.91 SGFDD50 pKa = 3.54 QKK52 pKa = 11.4 CLTFGDD58 pKa = 4.27 DD59 pKa = 3.99 PGTGAATTTDD69 pKa = 5.02 DD70 pKa = 4.3 IGIDD74 pKa = 3.57 QGDD77 pKa = 4.22 LEE79 pKa = 4.87 LLFAEE84 pKa = 4.93 EE85 pKa = 4.75 DD86 pKa = 3.51 ADD88 pKa = 4.03 GG89 pKa = 4.18
Molecular weight: 9.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.264
IPC2_protein 4.253
IPC_protein 4.19
Toseland 3.986
ProMoST 4.355
Dawson 4.19
Bjellqvist 4.342
Wikipedia 4.126
Rodwell 4.024
Grimsley 3.897
Solomon 4.177
Lehninger 4.139
Nozaki 4.304
DTASelect 4.558
Thurlkill 4.037
EMBOSS 4.139
Sillero 4.317
Patrickios 3.503
IPC_peptide 4.177
IPC2_peptide 4.291
IPC2.peptide.svr19 4.244
Protein with the highest isoelectric point:
>tr|Q9JG53|Q9JG53_9VIRU Capsid protein OS=Torque teno mini virus 2 OX=687370 PE=3 SV=1
MM1 pKa = 7.21 QCTLFISSSEE11 pKa = 4.16 DD12 pKa = 3.16 ALLQCKK18 pKa = 7.36 TLKK21 pKa = 10.08 TPANNPNSPCPVTSYY36 pKa = 10.82 KK37 pKa = 10.22 QYY39 pKa = 11.2 KK40 pKa = 10.17 SLIQQQTNNQCSTTLTNEE58 pKa = 4.75 DD59 pKa = 2.52 IHH61 pKa = 6.69 LQKK64 pKa = 10.51 QLRR67 pKa = 11.84 KK68 pKa = 8.68 EE69 pKa = 4.1 LKK71 pKa = 8.72 RR72 pKa = 11.84 LKK74 pKa = 9.03 TVKK77 pKa = 9.98 HH78 pKa = 6.51 LYY80 pKa = 9.51 SQISSTHH87 pKa = 4.35 EE88 pKa = 4.23 SKK90 pKa = 10.8 RR91 pKa = 11.84 KK92 pKa = 7.22 RR93 pKa = 11.84 HH94 pKa = 5.08 RR95 pKa = 11.84 RR96 pKa = 11.84 TRR98 pKa = 11.84 ARR100 pKa = 11.84 KK101 pKa = 9.63 KK102 pKa = 7.99 PTHH105 pKa = 6.54 HH106 pKa = 6.33 KK107 pKa = 8.88 TSPSSSSTSDD117 pKa = 3.29 EE118 pKa = 4.3 GDD120 pKa = 3.45 SSSSEE125 pKa = 4.05 SSNN128 pKa = 3.21
Molecular weight: 14.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.077
IPC2_protein 9.18
IPC_protein 9.121
Toseland 10.145
ProMoST 9.663
Dawson 10.262
Bjellqvist 9.882
Wikipedia 10.365
Rodwell 10.891
Grimsley 10.292
Solomon 10.292
Lehninger 10.277
Nozaki 10.175
DTASelect 9.853
Thurlkill 10.145
EMBOSS 10.511
Sillero 10.189
Patrickios 10.643
IPC_peptide 10.292
IPC2_peptide 8.58
IPC2.peptide.svr19 8.114
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
845
89
628
281.7
32.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.195 ± 0.551
2.012 ± 0.866
5.799 ± 1.746
3.905 ± 0.602
4.379 ± 1.329
4.615 ± 1.678
3.077 ± 0.55
5.917 ± 1.224
8.047 ± 1.059
8.047 ± 0.887
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.302 ± 0.215
5.089 ± 0.928
7.337 ± 1.422
5.562 ± 0.793
6.154 ± 1.224
7.219 ± 3.723
8.639 ± 0.798
2.367 ± 0.313
2.604 ± 1.008
4.734 ± 1.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here