Paenibacillus sp. GP183

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H4V018|A0A1H4V018_9BACL Methyl-accepting chemotaxis protein OS=Paenibacillus sp. GP183 OX=1882751 GN=SAMN05443246_5199 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 10.35KK3 pKa = 9.62PQEE6 pKa = 4.11GLLRR10 pKa = 11.84NAYY13 pKa = 10.03GDD15 pKa = 5.14DD16 pKa = 3.58IILYY20 pKa = 9.53DD21 pKa = 3.95EE22 pKa = 4.51QGGSTVYY29 pKa = 10.51QLLAEE34 pKa = 4.41FVYY37 pKa = 10.9GNYY40 pKa = 9.49TYY42 pKa = 11.35AVLQSEE48 pKa = 4.58EE49 pKa = 4.23LKK51 pKa = 11.09KK52 pKa = 10.98EE53 pKa = 4.15DD54 pKa = 2.89VDD56 pKa = 3.89VAIFRR61 pKa = 11.84VVEE64 pKa = 4.02HH65 pKa = 7.33AEE67 pKa = 3.79QQYY70 pKa = 10.28EE71 pKa = 4.28LEE73 pKa = 4.5TIEE76 pKa = 6.46DD77 pKa = 3.79DD78 pKa = 5.45DD79 pKa = 3.6EE80 pKa = 4.65WEE82 pKa = 4.6TVSEE86 pKa = 4.97LYY88 pKa = 10.82DD89 pKa = 3.44EE90 pKa = 4.58MMFPVKK96 pKa = 10.4EE97 pKa = 4.04ILL99 pKa = 3.63

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H4RNQ3|A0A1H4RNQ3_9BACL UDP-N-acetylglucosamine 1-carboxyvinyltransferase OS=Paenibacillus sp. GP183 OX=1882751 GN=murA PE=3 SV=1
MM1 pKa = 7.61GPTFNPNNRR10 pKa = 11.84KK11 pKa = 9.34RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 8.97NHH16 pKa = 5.01GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 9.99NGRR28 pKa = 11.84KK29 pKa = 8.83VLSARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 8.85VISAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5493

0

5493

1600958

29

2731

291.5

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.66 ± 0.034

0.802 ± 0.009

5.023 ± 0.022

6.354 ± 0.041

4.317 ± 0.027

7.076 ± 0.031

2.205 ± 0.018

7.35 ± 0.033

5.97 ± 0.032

10.171 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.879 ± 0.017

4.134 ± 0.025

4.034 ± 0.024

4.034 ± 0.026

4.704 ± 0.031

6.498 ± 0.026

5.32 ± 0.029

6.854 ± 0.026

1.24 ± 0.014

3.377 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski