Wenling sobemo-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF38|A0A1L3KF38_9VIRU RNA-directed RNA polymerase OS=Wenling sobemo-like virus 2 OX=1923541 PE=4 SV=1
MM1 pKa = 8.0EE2 pKa = 5.88IPDD5 pKa = 3.79DD6 pKa = 3.5WRR8 pKa = 11.84SDD10 pKa = 3.47VRR12 pKa = 11.84IDD14 pKa = 3.77QAIDD18 pKa = 3.34SLQGNSSPGVPFAHH32 pKa = 6.1VPSNNILRR40 pKa = 11.84EE41 pKa = 4.03KK42 pKa = 10.94YY43 pKa = 9.7RR44 pKa = 11.84GQLVTLVKK52 pKa = 10.41QRR54 pKa = 11.84LDD56 pKa = 3.47GNLPAFPIRR65 pKa = 11.84LFVKK69 pKa = 10.03PEE71 pKa = 3.28WHH73 pKa = 6.94KK74 pKa = 10.79IEE76 pKa = 4.78KK77 pKa = 9.88LRR79 pKa = 11.84QQRR82 pKa = 11.84YY83 pKa = 8.37RR84 pKa = 11.84LISQVSVVDD93 pKa = 3.54QVIDD97 pKa = 3.61KK98 pKa = 10.75LLVASYY104 pKa = 10.87YY105 pKa = 10.68DD106 pKa = 3.91YY107 pKa = 11.61EE108 pKa = 4.13NSHH111 pKa = 7.07YY112 pKa = 10.13IRR114 pKa = 11.84QPGKK118 pKa = 10.64VGWSYY123 pKa = 11.89VGGGYY128 pKa = 10.71KK129 pKa = 10.29LVPRR133 pKa = 11.84GWGYY137 pKa = 10.82DD138 pKa = 3.1RR139 pKa = 11.84KK140 pKa = 10.33AWDD143 pKa = 3.22WSVPSWLLEE152 pKa = 4.29DD153 pKa = 4.14YY154 pKa = 11.26EE155 pKa = 5.19FFLEE159 pKa = 4.23NQITSTKK166 pKa = 9.63DD167 pKa = 2.86HH168 pKa = 7.03KK169 pKa = 11.07DD170 pKa = 2.85WLRR173 pKa = 11.84KK174 pKa = 9.48RR175 pKa = 11.84FAEE178 pKa = 4.5LYY180 pKa = 8.41EE181 pKa = 4.37RR182 pKa = 11.84PILQLSDD189 pKa = 3.26GTLLQQLVSGVQKK202 pKa = 10.71SGAYY206 pKa = 7.44YY207 pKa = 9.59TLNGNTTMQVLLHH220 pKa = 6.53HH221 pKa = 6.75ATTLEE226 pKa = 4.1SGEE229 pKa = 5.05DD230 pKa = 3.87STPMIALGDD239 pKa = 3.89DD240 pKa = 3.81TVQPLASPAYY250 pKa = 10.62LEE252 pKa = 4.23VLQRR256 pKa = 11.84WVTLKK261 pKa = 10.56EE262 pKa = 3.73AVPNEE267 pKa = 4.2FCGMSYY273 pKa = 10.22SGEE276 pKa = 4.08NVEE279 pKa = 5.47PVYY282 pKa = 10.52QDD284 pKa = 3.01KK285 pKa = 10.95HH286 pKa = 7.69AMNLAADD293 pKa = 4.77LSQDD297 pKa = 3.2TLLQYY302 pKa = 10.96HH303 pKa = 7.13LLYY306 pKa = 10.6GQSTKK311 pKa = 10.66LPKK314 pKa = 10.04LRR316 pKa = 11.84RR317 pKa = 11.84LNALAGHH324 pKa = 6.67PPVHH328 pKa = 5.94QLWLSTIWGG337 pKa = 3.72

Molecular weight: 38.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF38|A0A1L3KF38_9VIRU RNA-directed RNA polymerase OS=Wenling sobemo-like virus 2 OX=1923541 PE=4 SV=1
MM1 pKa = 7.32FKK3 pKa = 10.61VIAIGVVLATLVALARR19 pKa = 11.84YY20 pKa = 8.7RR21 pKa = 11.84WDD23 pKa = 3.38YY24 pKa = 10.87LVTMGALAMVFVMLVWEE41 pKa = 4.14LLFRR45 pKa = 11.84FWQGALVPLYY55 pKa = 10.45EE56 pKa = 4.59SLLEE60 pKa = 4.03PLLVGDD66 pKa = 4.79DD67 pKa = 4.04PTPIFYY73 pKa = 9.67TPTPTTMEE81 pKa = 4.56LTPMMIYY88 pKa = 10.47FMIVAATVILVTLIVRR104 pKa = 11.84CTPSSRR110 pKa = 11.84IIYY113 pKa = 9.73SPEE116 pKa = 3.62AMVHH120 pKa = 5.97GSAFEE125 pKa = 3.96ALPPPKK131 pKa = 10.43CQVTLVAVTGTEE143 pKa = 3.9VSTGQGALIKK153 pKa = 10.1YY154 pKa = 8.98GEE156 pKa = 4.54GVAVVTALHH165 pKa = 5.38VVKK168 pKa = 10.42GASVVLAEE176 pKa = 4.09NRR178 pKa = 11.84EE179 pKa = 4.09GLSIRR184 pKa = 11.84LGARR188 pKa = 11.84KK189 pKa = 9.64VIHH192 pKa = 6.18QDD194 pKa = 2.85GVAFPVEE201 pKa = 3.99AAQMSRR207 pKa = 11.84LGVRR211 pKa = 11.84VGQTTVVTSPVAASAHH227 pKa = 4.65GVMGRR232 pKa = 11.84SSGCLMQISDD242 pKa = 4.87GLLSYY247 pKa = 10.52QGSTQPGYY255 pKa = 10.55SGAPVMVGGKK265 pKa = 9.35VAGIHH270 pKa = 6.16IGTVEE275 pKa = 3.9GRR277 pKa = 11.84NLAFDD282 pKa = 3.54ISLAISRR289 pKa = 11.84SNDD292 pKa = 2.92YY293 pKa = 10.42RR294 pKa = 11.84YY295 pKa = 10.62NPEE298 pKa = 3.82AQTTSEE304 pKa = 3.85MTPRR308 pKa = 11.84EE309 pKa = 3.69KK310 pKa = 10.61GYY312 pKa = 10.78YY313 pKa = 10.13GGMDD317 pKa = 3.12ARR319 pKa = 11.84FKK321 pKa = 11.01KK322 pKa = 10.53GLAKK326 pKa = 10.61LEE328 pKa = 4.28EE329 pKa = 4.38QDD331 pKa = 4.3DD332 pKa = 4.32QPGSWAHH339 pKa = 5.95IPEE342 pKa = 4.84EE343 pKa = 3.8IDD345 pKa = 3.3YY346 pKa = 10.98SRR348 pKa = 11.84KK349 pKa = 8.54LTFEE353 pKa = 3.91NQMLSLTKK361 pKa = 10.35SVAALEE367 pKa = 4.17EE368 pKa = 4.55TVAKK372 pKa = 9.8MDD374 pKa = 3.41FRR376 pKa = 11.84RR377 pKa = 11.84RR378 pKa = 11.84RR379 pKa = 11.84GSTRR383 pKa = 11.84RR384 pKa = 11.84HH385 pKa = 5.51LSSSSEE391 pKa = 4.23SSCSSSEE398 pKa = 3.9SSRR401 pKa = 11.84DD402 pKa = 2.98EE403 pKa = 4.19DD404 pKa = 3.48RR405 pKa = 11.84GSRR408 pKa = 11.84PSQKK412 pKa = 10.51SKK414 pKa = 8.64NHH416 pKa = 6.3RR417 pKa = 11.84KK418 pKa = 9.63RR419 pKa = 11.84PSKK422 pKa = 10.2HH423 pKa = 5.85LRR425 pKa = 11.84RR426 pKa = 11.84QASRR430 pKa = 11.84ISRR433 pKa = 11.84KK434 pKa = 9.66SVVVTVPHH442 pKa = 6.88PSEE445 pKa = 4.77PPPKK449 pKa = 9.34PGPSGLTSSTSGKK462 pKa = 10.54GPAHH466 pKa = 6.45PKK468 pKa = 10.06KK469 pKa = 10.85SEE471 pKa = 3.8GKK473 pKa = 9.0QQ474 pKa = 3.09

Molecular weight: 51.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

811

337

474

405.5

45.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.782 ± 1.101

0.617 ± 0.203

4.316 ± 1.214

5.425 ± 0.241

2.343 ± 0.168

7.645 ± 0.708

2.589 ± 0.24

4.069 ± 0.134

4.932 ± 0.071

10.111 ± 1.679

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.779

2.219 ± 0.85

6.165 ± 0.334

4.686 ± 1.168

6.165 ± 0.522

9.125 ± 1.269

5.672 ± 0.774

8.631 ± 0.769

1.85 ± 0.896

3.946 ± 0.884
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski