Equus caballus papillomavirus 8
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D9EPU1|A0A1D9EPU1_9PAPI Replication protein E1 OS=Equus caballus papillomavirus 8 OX=1912759 GN=E1 PE=3 SV=1
MM1 pKa = 7.99 RR2 pKa = 11.84 GRR4 pKa = 11.84 NVTLRR9 pKa = 11.84 DD10 pKa = 3.17 IVLEE14 pKa = 3.95 EE15 pKa = 4.12 RR16 pKa = 11.84 PQLVDD21 pKa = 3.4 LHH23 pKa = 8.17 CDD25 pKa = 3.16 EE26 pKa = 4.53 QLPPEE31 pKa = 4.28 EE32 pKa = 4.55 EE33 pKa = 4.18 EE34 pKa = 4.49 EE35 pKa = 4.03 EE36 pKa = 4.28 VANYY40 pKa = 10.49 EE41 pKa = 4.25 EE42 pKa = 4.67 VVEE45 pKa = 4.12 VTPYY49 pKa = 10.33 RR50 pKa = 11.84 VALPCGVCASPLRR63 pKa = 11.84 LFIQATDD70 pKa = 3.15 EE71 pKa = 4.12 GRR73 pKa = 11.84 RR74 pKa = 11.84 GLEE77 pKa = 3.48 LLLFRR82 pKa = 11.84 DD83 pKa = 4.26 LAIICPSCARR93 pKa = 11.84 NEE95 pKa = 4.25 TRR97 pKa = 11.84 HH98 pKa = 5.13 EE99 pKa = 4.01
Molecular weight: 11.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.588
IPC2_protein 4.635
IPC_protein 4.507
Toseland 4.368
ProMoST 4.52
Dawson 4.431
Bjellqvist 4.622
Wikipedia 4.253
Rodwell 4.342
Grimsley 4.279
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.609
Thurlkill 4.355
EMBOSS 4.279
Sillero 4.609
Patrickios 3.668
IPC_peptide 4.431
IPC2_peptide 4.609
IPC2.peptide.svr19 4.535
Protein with the highest isoelectric point:
>tr|A0A1D9EPW8|A0A1D9EPW8_9PAPI Regulatory protein E2 OS=Equus caballus papillomavirus 8 OX=1912759 GN=E2 PE=3 SV=1
MM1 pKa = 7.47 EE2 pKa = 5.5 RR3 pKa = 11.84 PRR5 pKa = 11.84 TLTDD9 pKa = 3.67 LSRR12 pKa = 11.84 QLGLGLTEE20 pKa = 5.64 INIQCIFCKK29 pKa = 10.37 FWLRR33 pKa = 11.84 RR34 pKa = 11.84 IDD36 pKa = 3.56 LSTFIRR42 pKa = 11.84 KK43 pKa = 8.81 SLNLAWRR50 pKa = 11.84 GGYY53 pKa = 9.39 PYY55 pKa = 10.58 ACCRR59 pKa = 11.84 SCSKK63 pKa = 10.87 VIAEE67 pKa = 4.34 RR68 pKa = 11.84 EE69 pKa = 3.85 RR70 pKa = 11.84 NQYY73 pKa = 10.85 YY74 pKa = 7.11 EE75 pKa = 4.29 TSAKK79 pKa = 9.61 GPRR82 pKa = 11.84 VEE84 pKa = 4.48 RR85 pKa = 11.84 LHH87 pKa = 6.19 GRR89 pKa = 11.84 PIGEE93 pKa = 3.72 IAVRR97 pKa = 11.84 CILCLSLLSYY107 pKa = 10.14 FEE109 pKa = 4.12 KK110 pKa = 10.69 VDD112 pKa = 3.8 VIEE115 pKa = 4.89 RR116 pKa = 11.84 GEE118 pKa = 3.88 SFYY121 pKa = 10.9 RR122 pKa = 11.84 VRR124 pKa = 11.84 DD125 pKa = 3.4 RR126 pKa = 11.84 WRR128 pKa = 11.84 AKK130 pKa = 10.23 CRR132 pKa = 11.84 EE133 pKa = 4.0 CRR135 pKa = 11.84 AQQDD139 pKa = 2.96 ARR141 pKa = 11.84 AA142 pKa = 3.76
Molecular weight: 16.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.986
IPC2_protein 8.887
IPC_protein 8.99
Toseland 9.268
ProMoST 9.297
Dawson 9.648
Bjellqvist 9.633
Wikipedia 9.838
Rodwell 9.736
Grimsley 9.677
Solomon 9.736
Lehninger 9.706
Nozaki 9.794
DTASelect 9.487
Thurlkill 9.545
EMBOSS 9.78
Sillero 9.75
Patrickios 4.863
IPC_peptide 9.721
IPC2_peptide 9.107
IPC2.peptide.svr19 7.784
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2560
99
709
426.7
47.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.797 ± 0.65
2.07 ± 0.537
5.234 ± 0.614
5.938 ± 0.71
3.789 ± 0.48
8.945 ± 1.422
2.266 ± 0.288
2.969 ± 0.712
3.477 ± 0.655
8.438 ± 0.423
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.523 ± 0.379
3.086 ± 0.461
8.516 ± 1.568
4.883 ± 0.773
7.383 ± 0.757
8.281 ± 0.799
5.469 ± 0.402
6.406 ± 0.922
1.953 ± 0.298
2.578 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here