Penicillium aurantiogriseum bipartite virus 1
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2KPE1|A0A0S2KPE1_9VIRU 20 kDa protein OS=Penicillium aurantiogriseum bipartite virus 1 OX=1755754 PE=4 SV=1
MM1 pKa = 7.49 VGVGSDD7 pKa = 3.01 TDD9 pKa = 4.04 GNDD12 pKa = 2.95 EE13 pKa = 4.34 HH14 pKa = 9.29 SYY16 pKa = 8.8 EE17 pKa = 4.05 QWRR20 pKa = 11.84 TTPLDD25 pKa = 3.58 VIIARR30 pKa = 11.84 DD31 pKa = 3.42 WVVEE35 pKa = 3.88 LEE37 pKa = 4.09 QGPMEE42 pKa = 4.81 LMAEE46 pKa = 4.38 LGLRR50 pKa = 11.84 SPSYY54 pKa = 10.66 PDD56 pKa = 4.19 LEE58 pKa = 4.7 HH59 pKa = 6.55 HH60 pKa = 6.5 ATWISDD66 pKa = 3.59 DD67 pKa = 4.63 DD68 pKa = 5.29 GDD70 pKa = 3.92 VTRR73 pKa = 11.84 TGVPYY78 pKa = 10.49 RR79 pKa = 11.84 GEE81 pKa = 3.78 NLFAQWVLEE90 pKa = 4.03 TLLGEE95 pKa = 4.18 SRR97 pKa = 11.84 MDD99 pKa = 3.37 VEE101 pKa = 4.51 LTAEE105 pKa = 4.31 VAAEE109 pKa = 4.01 MGVMAVAGYY118 pKa = 9.7 SAAVWLQGEE127 pKa = 4.58 VIDD130 pKa = 5.46 LRR132 pKa = 11.84 VLRR135 pKa = 11.84 VASTPLAQAYY145 pKa = 7.8 HH146 pKa = 6.63 ALVAGKK152 pKa = 10.0 SAVDD156 pKa = 3.59 EE157 pKa = 4.19 KK158 pKa = 11.37 HH159 pKa = 6.66 FMGGSANN166 pKa = 3.49
Molecular weight: 18.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.343
IPC2_protein 4.279
IPC_protein 4.215
Toseland 4.05
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.062
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.126
Nozaki 4.291
DTASelect 4.457
Thurlkill 4.075
EMBOSS 4.088
Sillero 4.342
Patrickios 3.617
IPC_peptide 4.177
IPC2_peptide 4.329
IPC2.peptide.svr19 4.234
Protein with the highest isoelectric point:
>tr|A0A0S2KPE1|A0A0S2KPE1_9VIRU 20 kDa protein OS=Penicillium aurantiogriseum bipartite virus 1 OX=1755754 PE=4 SV=1
MM1 pKa = 7.92 ADD3 pKa = 3.1 MTAPPSFLDD12 pKa = 3.4 KK13 pKa = 11.12 FGGSTANVDD22 pKa = 3.74 YY23 pKa = 10.96 TDD25 pKa = 5.13 DD26 pKa = 3.92 PSKK29 pKa = 10.92 QGGILPIPLQGDD41 pKa = 3.68 SLATLEE47 pKa = 4.31 TKK49 pKa = 9.22 MRR51 pKa = 11.84 NLARR55 pKa = 11.84 VVEE58 pKa = 4.99 RR59 pKa = 11.84 GASVARR65 pKa = 11.84 FTGMMGTPQQSRR77 pKa = 11.84 DD78 pKa = 3.41 ALQTAKK84 pKa = 10.02 MRR86 pKa = 11.84 MTEE89 pKa = 3.93 VEE91 pKa = 4.4 KK92 pKa = 11.03 EE93 pKa = 4.21 MFSHH97 pKa = 6.56 WEE99 pKa = 3.68 AGGRR103 pKa = 11.84 LPDD106 pKa = 4.03 VNWGTDD112 pKa = 3.18 VDD114 pKa = 4.18 AMPSGSEE121 pKa = 3.84 PRR123 pKa = 11.84 RR124 pKa = 11.84 KK125 pKa = 10.23 AEE127 pKa = 3.9 IQAEE131 pKa = 4.11 ALNRR135 pKa = 11.84 LYY137 pKa = 9.97 KK138 pKa = 10.14 TDD140 pKa = 3.49 AATAGHH146 pKa = 5.35 VVHH149 pKa = 7.02 WKK151 pKa = 7.86 KK152 pKa = 8.86 TFRR155 pKa = 11.84 AIIPLLVSMTKK166 pKa = 10.1 VVGSEE171 pKa = 3.7 RR172 pKa = 11.84 DD173 pKa = 3.4 FLGGQDD179 pKa = 3.52 VLDD182 pKa = 4.5 ANEE185 pKa = 3.93 MAEE188 pKa = 4.02 VQTIGKK194 pKa = 9.73 VNGTTYY200 pKa = 11.44 ASINAIQEE208 pKa = 4.06 EE209 pKa = 4.64 VHH211 pKa = 6.65 RR212 pKa = 11.84 LLSSINRR219 pKa = 11.84 SKK221 pKa = 11.43 DD222 pKa = 3.17 LLQARR227 pKa = 11.84 EE228 pKa = 4.02 HH229 pKa = 6.31 ALKK232 pKa = 10.75 AKK234 pKa = 9.53 NPKK237 pKa = 10.08 YY238 pKa = 10.12 KK239 pKa = 10.52 VKK241 pKa = 10.63 QSPANDD247 pKa = 2.9 ARR249 pKa = 11.84 QLLGKK254 pKa = 9.82 RR255 pKa = 11.84 RR256 pKa = 11.84 LGADD260 pKa = 3.03 VDD262 pKa = 5.14 FSADD266 pKa = 3.91 PIPEE270 pKa = 3.6 RR271 pKa = 11.84 RR272 pKa = 11.84 VRR274 pKa = 11.84 RR275 pKa = 11.84 NLALGVHH282 pKa = 5.39 QEE284 pKa = 4.26 SVPIASRR291 pKa = 11.84 AGEE294 pKa = 3.83 QRR296 pKa = 11.84 APTRR300 pKa = 11.84 GGINQRR306 pKa = 11.84 RR307 pKa = 11.84 PHH309 pKa = 7.31 PDD311 pKa = 3.13 TDD313 pKa = 3.77 PSADD317 pKa = 3.54 MDD319 pKa = 4.02 TQII322 pKa = 5.11
Molecular weight: 35.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.744
IPC2_protein 8.463
IPC_protein 8.478
Toseland 9.414
ProMoST 9.004
Dawson 9.589
Bjellqvist 9.194
Wikipedia 9.721
Rodwell 9.955
Grimsley 9.648
Solomon 9.677
Lehninger 9.648
Nozaki 9.355
DTASelect 9.209
Thurlkill 9.443
EMBOSS 9.809
Sillero 9.502
Patrickios 7.673
IPC_peptide 9.677
IPC2_peptide 7.629
IPC2.peptide.svr19 7.684
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1102
166
614
367.3
41.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.53 ± 1.296
0.454 ± 0.276
7.26 ± 0.149
7.169 ± 0.686
3.085 ± 0.78
6.897 ± 0.735
2.269 ± 0.197
4.446 ± 0.666
4.537 ± 0.869
8.53 ± 0.453
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.722 ± 0.72
2.904 ± 0.372
5.626 ± 0.531
4.628 ± 0.42
7.078 ± 0.669
5.626 ± 0.319
5.898 ± 0.15
7.169 ± 0.96
1.996 ± 0.442
3.176 ± 0.869
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here