Amino acid dipepetide frequency for Serratia phage JS26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.177AlaAla: 12.177 ± 1.875
1.198AlaCys: 1.198 ± 0.292
5.889AlaAsp: 5.889 ± 0.529
6.837AlaGlu: 6.837 ± 0.662
2.745AlaPhe: 2.745 ± 0.37
7.885AlaGly: 7.885 ± 0.668
1.447AlaHis: 1.447 ± 0.241
4.891AlaIle: 4.891 ± 0.552
4.741AlaLys: 4.741 ± 0.588
8.234AlaLeu: 8.234 ± 0.826
2.944AlaMet: 2.944 ± 0.39
4.192AlaAsn: 4.192 ± 0.472
3.943AlaPro: 3.943 ± 0.477
4.092AlaGln: 4.092 ± 0.627
5.49AlaArg: 5.49 ± 0.54
4.941AlaSer: 4.941 ± 0.603
4.991AlaThr: 4.991 ± 0.674
6.787AlaVal: 6.787 ± 0.796
1.946AlaTrp: 1.946 ± 0.346
3.144AlaTyr: 3.144 ± 0.352
0.0AlaXaa: 0.0 ± 0.0
Cys
0.499CysAla: 0.499 ± 0.171
0.349CysCys: 0.349 ± 0.21
0.948CysAsp: 0.948 ± 0.235
0.449CysGlu: 0.449 ± 0.16
0.15CysPhe: 0.15 ± 0.091
0.499CysGly: 0.499 ± 0.183
0.299CysHis: 0.299 ± 0.104
0.399CysIle: 0.399 ± 0.127
0.299CysLys: 0.299 ± 0.13
0.898CysLeu: 0.898 ± 0.219
0.2CysMet: 0.2 ± 0.097
0.449CysAsn: 0.449 ± 0.144
0.749CysPro: 0.749 ± 0.228
0.499CysGln: 0.499 ± 0.154
0.898CysArg: 0.898 ± 0.21
0.649CysSer: 0.649 ± 0.223
0.549CysThr: 0.549 ± 0.143
1.048CysVal: 1.048 ± 0.24
0.1CysTrp: 0.1 ± 0.065
0.299CysTyr: 0.299 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
5.589AspAla: 5.589 ± 0.513
0.798AspCys: 0.798 ± 0.192
4.192AspAsp: 4.192 ± 0.7
4.442AspGlu: 4.442 ± 0.554
3.144AspPhe: 3.144 ± 0.46
5.34AspGly: 5.34 ± 0.456
1.996AspHis: 1.996 ± 0.332
3.843AspIle: 3.843 ± 0.473
3.543AspLys: 3.543 ± 0.399
4.941AspLeu: 4.941 ± 0.477
2.046AspMet: 2.046 ± 0.365
2.645AspAsn: 2.645 ± 0.373
2.845AspPro: 2.845 ± 0.456
2.645AspGln: 2.645 ± 0.368
3.344AspArg: 3.344 ± 0.492
2.695AspSer: 2.695 ± 0.351
3.493AspThr: 3.493 ± 0.566
4.442AspVal: 4.442 ± 0.528
1.647AspTrp: 1.647 ± 0.228
2.346AspTyr: 2.346 ± 0.349
0.0AspXaa: 0.0 ± 0.0
Glu
4.891GluAla: 4.891 ± 0.698
0.549GluCys: 0.549 ± 0.17
3.843GluAsp: 3.843 ± 0.45
3.893GluGlu: 3.893 ± 0.484
2.046GluPhe: 2.046 ± 0.246
3.543GluGly: 3.543 ± 0.433
1.098GluHis: 1.098 ± 0.257
3.643GluIle: 3.643 ± 0.432
3.643GluLys: 3.643 ± 0.457
5.19GluLeu: 5.19 ± 0.441
2.795GluMet: 2.795 ± 0.422
2.895GluAsn: 2.895 ± 0.4
1.697GluPro: 1.697 ± 0.335
3.443GluGln: 3.443 ± 0.51
3.394GluArg: 3.394 ± 0.453
3.194GluSer: 3.194 ± 0.371
3.493GluThr: 3.493 ± 0.363
3.843GluVal: 3.843 ± 0.429
1.797GluTrp: 1.797 ± 0.311
1.996GluTyr: 1.996 ± 0.254
0.0GluXaa: 0.0 ± 0.0
Phe
2.895PheAla: 2.895 ± 0.429
0.2PheCys: 0.2 ± 0.099
2.845PheAsp: 2.845 ± 0.375
2.196PheGlu: 2.196 ± 0.347
0.948PhePhe: 0.948 ± 0.228
2.795PheGly: 2.795 ± 0.42
0.349PheHis: 0.349 ± 0.129
1.846PheIle: 1.846 ± 0.289
1.996PheLys: 1.996 ± 0.308
1.846PheLeu: 1.846 ± 0.315
1.198PheMet: 1.198 ± 0.224
1.697PheAsn: 1.697 ± 0.289
1.447PhePro: 1.447 ± 0.385
0.998PheGln: 0.998 ± 0.287
1.397PheArg: 1.397 ± 0.254
2.695PheSer: 2.695 ± 0.324
2.745PheThr: 2.745 ± 0.34
2.795PheVal: 2.795 ± 0.395
0.549PheTrp: 0.549 ± 0.17
1.347PheTyr: 1.347 ± 0.257
0.0PheXaa: 0.0 ± 0.0
Gly
7.236GlyAla: 7.236 ± 0.708
0.749GlyCys: 0.749 ± 0.212
4.791GlyAsp: 4.791 ± 0.465
3.743GlyGlu: 3.743 ± 0.41
2.895GlyPhe: 2.895 ± 0.388
6.088GlyGly: 6.088 ± 1.063
0.948GlyHis: 0.948 ± 0.196
3.693GlyIle: 3.693 ± 0.505
5.34GlyLys: 5.34 ± 0.555
4.541GlyLeu: 4.541 ± 0.348
2.595GlyMet: 2.595 ± 0.354
2.595GlyAsn: 2.595 ± 0.398
1.846GlyPro: 1.846 ± 0.317
3.144GlyGln: 3.144 ± 0.331
3.643GlyArg: 3.643 ± 0.418
4.591GlySer: 4.591 ± 0.536
5.24GlyThr: 5.24 ± 0.647
5.49GlyVal: 5.49 ± 0.533
1.347GlyTrp: 1.347 ± 0.253
2.695GlyTyr: 2.695 ± 0.305
0.0GlyXaa: 0.0 ± 0.0
His
1.148HisAla: 1.148 ± 0.287
0.15HisCys: 0.15 ± 0.081
1.298HisAsp: 1.298 ± 0.283
0.848HisGlu: 0.848 ± 0.188
0.649HisPhe: 0.649 ± 0.195
1.447HisGly: 1.447 ± 0.273
0.549HisHis: 0.549 ± 0.183
1.048HisIle: 1.048 ± 0.249
0.699HisLys: 0.699 ± 0.171
1.447HisLeu: 1.447 ± 0.287
0.599HisMet: 0.599 ± 0.148
0.848HisAsn: 0.848 ± 0.225
1.048HisPro: 1.048 ± 0.253
0.649HisGln: 0.649 ± 0.169
1.048HisArg: 1.048 ± 0.245
0.699HisSer: 0.699 ± 0.175
0.948HisThr: 0.948 ± 0.244
1.048HisVal: 1.048 ± 0.191
0.549HisTrp: 0.549 ± 0.177
0.848HisTyr: 0.848 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.34IleAla: 5.34 ± 0.508
0.699IleCys: 0.699 ± 0.159
4.641IleAsp: 4.641 ± 0.53
4.342IleGlu: 4.342 ± 0.375
1.298IlePhe: 1.298 ± 0.227
2.545IleGly: 2.545 ± 0.272
0.699IleHis: 0.699 ± 0.166
2.895IleIle: 2.895 ± 0.508
2.795IleLys: 2.795 ± 0.303
3.643IleLeu: 3.643 ± 0.41
1.447IleMet: 1.447 ± 0.232
2.645IleAsn: 2.645 ± 0.388
2.096IlePro: 2.096 ± 0.358
1.647IleGln: 1.647 ± 0.261
3.493IleArg: 3.493 ± 0.37
2.595IleSer: 2.595 ± 0.404
4.541IleThr: 4.541 ± 0.474
3.194IleVal: 3.194 ± 0.44
0.449IleTrp: 0.449 ± 0.126
1.797IleTyr: 1.797 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
6.288LysAla: 6.288 ± 0.633
0.499LysCys: 0.499 ± 0.163
3.643LysAsp: 3.643 ± 0.517
3.643LysGlu: 3.643 ± 0.482
1.797LysPhe: 1.797 ± 0.29
4.392LysGly: 4.392 ± 0.526
1.098LysHis: 1.098 ± 0.244
2.495LysIle: 2.495 ± 0.334
3.943LysLys: 3.943 ± 0.619
4.142LysLeu: 4.142 ± 0.461
2.246LysMet: 2.246 ± 0.251
1.347LysAsn: 1.347 ± 0.247
3.094LysPro: 3.094 ± 0.445
1.946LysGln: 1.946 ± 0.329
3.344LysArg: 3.344 ± 0.428
2.895LysSer: 2.895 ± 0.488
3.194LysThr: 3.194 ± 0.472
3.493LysVal: 3.493 ± 0.42
0.898LysTrp: 0.898 ± 0.234
1.597LysTyr: 1.597 ± 0.299
0.0LysXaa: 0.0 ± 0.0
Leu
6.637LeuAla: 6.637 ± 0.66
0.848LeuCys: 0.848 ± 0.215
4.941LeuAsp: 4.941 ± 0.537
5.09LeuGlu: 5.09 ± 0.513
2.096LeuPhe: 2.096 ± 0.315
5.24LeuGly: 5.24 ± 0.561
1.298LeuHis: 1.298 ± 0.221
4.292LeuIle: 4.292 ± 0.403
4.142LeuLys: 4.142 ± 0.571
5.789LeuLeu: 5.789 ± 0.665
2.296LeuMet: 2.296 ± 0.338
3.194LeuAsn: 3.194 ± 0.486
3.693LeuPro: 3.693 ± 0.466
3.893LeuGln: 3.893 ± 0.513
4.991LeuArg: 4.991 ± 0.514
3.992LeuSer: 3.992 ± 0.425
6.238LeuThr: 6.238 ± 0.557
5.24LeuVal: 5.24 ± 0.582
0.948LeuTrp: 0.948 ± 0.256
2.296LeuTyr: 2.296 ± 0.349
0.0LeuXaa: 0.0 ± 0.0
Met
3.943MetAla: 3.943 ± 0.477
0.299MetCys: 0.299 ± 0.12
2.046MetAsp: 2.046 ± 0.308
1.098MetGlu: 1.098 ± 0.267
0.998MetPhe: 0.998 ± 0.23
2.096MetGly: 2.096 ± 0.298
0.449MetHis: 0.449 ± 0.147
1.397MetIle: 1.397 ± 0.29
2.296MetLys: 2.296 ± 0.345
2.545MetLeu: 2.545 ± 0.314
0.948MetMet: 0.948 ± 0.287
1.198MetAsn: 1.198 ± 0.206
2.196MetPro: 2.196 ± 0.36
1.248MetGln: 1.248 ± 0.248
1.797MetArg: 1.797 ± 0.324
1.697MetSer: 1.697 ± 0.294
2.645MetThr: 2.645 ± 0.366
2.046MetVal: 2.046 ± 0.304
0.399MetTrp: 0.399 ± 0.133
0.749MetTyr: 0.749 ± 0.182
0.0MetXaa: 0.0 ± 0.0
Asn
3.992AsnAla: 3.992 ± 0.559
0.299AsnCys: 0.299 ± 0.12
2.445AsnAsp: 2.445 ± 0.319
1.946AsnGlu: 1.946 ± 0.278
1.048AsnPhe: 1.048 ± 0.248
4.442AsnGly: 4.442 ± 0.438
0.699AsnHis: 0.699 ± 0.201
2.096AsnIle: 2.096 ± 0.325
2.146AsnLys: 2.146 ± 0.395
3.344AsnLeu: 3.344 ± 0.439
0.998AsnMet: 0.998 ± 0.202
1.597AsnAsn: 1.597 ± 0.287
2.545AsnPro: 2.545 ± 0.331
1.048AsnGln: 1.048 ± 0.196
2.296AsnArg: 2.296 ± 0.328
2.046AsnSer: 2.046 ± 0.299
2.495AsnThr: 2.495 ± 0.365
2.046AsnVal: 2.046 ± 0.299
0.798AsnTrp: 0.798 ± 0.206
0.998AsnTyr: 0.998 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
3.843ProAla: 3.843 ± 0.392
0.549ProCys: 0.549 ± 0.156
4.192ProAsp: 4.192 ± 0.579
2.445ProGlu: 2.445 ± 0.467
1.747ProPhe: 1.747 ± 0.324
3.394ProGly: 3.394 ± 0.408
0.549ProHis: 0.549 ± 0.172
2.246ProIle: 2.246 ± 0.378
2.296ProLys: 2.296 ± 0.337
2.845ProLeu: 2.845 ± 0.393
1.347ProMet: 1.347 ± 0.295
1.497ProAsn: 1.497 ± 0.292
2.695ProPro: 2.695 ± 0.431
1.647ProGln: 1.647 ± 0.287
2.246ProArg: 2.246 ± 0.344
2.096ProSer: 2.096 ± 0.368
2.545ProThr: 2.545 ± 0.49
3.743ProVal: 3.743 ± 0.657
0.848ProTrp: 0.848 ± 0.216
1.896ProTyr: 1.896 ± 0.305
0.0ProXaa: 0.0 ± 0.0
Gln
5.34GlnAla: 5.34 ± 0.838
0.299GlnCys: 0.299 ± 0.104
2.046GlnAsp: 2.046 ± 0.491
2.495GlnGlu: 2.495 ± 0.387
1.797GlnPhe: 1.797 ± 0.28
2.495GlnGly: 2.495 ± 0.314
1.098GlnHis: 1.098 ± 0.227
2.296GlnIle: 2.296 ± 0.298
1.996GlnLys: 1.996 ± 0.361
3.344GlnLeu: 3.344 ± 0.46
1.347GlnMet: 1.347 ± 0.263
1.597GlnAsn: 1.597 ± 0.337
0.898GlnPro: 0.898 ± 0.243
1.946GlnGln: 1.946 ± 0.555
2.296GlnArg: 2.296 ± 0.335
2.645GlnSer: 2.645 ± 0.4
2.545GlnThr: 2.545 ± 0.366
2.695GlnVal: 2.695 ± 0.529
0.499GlnTrp: 0.499 ± 0.16
1.697GlnTyr: 1.697 ± 0.333
0.0GlnXaa: 0.0 ± 0.0
Arg
5.49ArgAla: 5.49 ± 0.57
0.599ArgCys: 0.599 ± 0.189
3.493ArgAsp: 3.493 ± 0.358
3.443ArgGlu: 3.443 ± 0.585
2.445ArgPhe: 2.445 ± 0.353
3.943ArgGly: 3.943 ± 0.41
0.948ArgHis: 0.948 ± 0.236
3.194ArgIle: 3.194 ± 0.391
3.743ArgLys: 3.743 ± 0.401
4.092ArgLeu: 4.092 ± 0.469
1.347ArgMet: 1.347 ± 0.307
1.896ArgAsn: 1.896 ± 0.3
2.395ArgPro: 2.395 ± 0.376
2.845ArgGln: 2.845 ± 0.48
3.943ArgArg: 3.943 ± 0.63
2.545ArgSer: 2.545 ± 0.371
2.795ArgThr: 2.795 ± 0.338
4.292ArgVal: 4.292 ± 0.381
0.898ArgTrp: 0.898 ± 0.262
2.246ArgTyr: 2.246 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
5.09SerAla: 5.09 ± 0.569
0.499SerCys: 0.499 ± 0.119
3.643SerAsp: 3.643 ± 0.447
2.545SerGlu: 2.545 ± 0.324
2.046SerPhe: 2.046 ± 0.332
4.741SerGly: 4.741 ± 0.604
0.948SerHis: 0.948 ± 0.218
2.895SerIle: 2.895 ± 0.343
2.445SerLys: 2.445 ± 0.391
4.192SerLeu: 4.192 ± 0.407
1.996SerMet: 1.996 ± 0.324
1.647SerAsn: 1.647 ± 0.305
2.445SerPro: 2.445 ± 0.414
2.645SerGln: 2.645 ± 0.469
3.044SerArg: 3.044 ± 0.423
3.194SerSer: 3.194 ± 0.431
3.294SerThr: 3.294 ± 0.488
3.743SerVal: 3.743 ± 0.513
0.798SerTrp: 0.798 ± 0.181
1.946SerTyr: 1.946 ± 0.318
0.0SerXaa: 0.0 ± 0.0
Thr
6.188ThrAla: 6.188 ± 0.643
0.549ThrCys: 0.549 ± 0.178
3.344ThrAsp: 3.344 ± 0.377
3.793ThrGlu: 3.793 ± 0.37
2.595ThrPhe: 2.595 ± 0.328
5.09ThrGly: 5.09 ± 0.573
1.098ThrHis: 1.098 ± 0.206
2.845ThrIle: 2.845 ± 0.398
3.843ThrLys: 3.843 ± 0.386
6.238ThrLeu: 6.238 ± 0.545
1.697ThrMet: 1.697 ± 0.263
2.495ThrAsn: 2.495 ± 0.361
3.893ThrPro: 3.893 ± 0.377
2.645ThrGln: 2.645 ± 0.312
3.044ThrArg: 3.044 ± 0.443
3.543ThrSer: 3.543 ± 0.423
4.541ThrThr: 4.541 ± 0.537
5.09ThrVal: 5.09 ± 0.592
1.048ThrTrp: 1.048 ± 0.214
1.697ThrTyr: 1.697 ± 0.28
0.0ThrXaa: 0.0 ± 0.0
Val
7.236ValAla: 7.236 ± 0.614
0.399ValCys: 0.399 ± 0.127
4.042ValAsp: 4.042 ± 0.414
4.591ValGlu: 4.591 ± 0.47
2.745ValPhe: 2.745 ± 0.428
3.893ValGly: 3.893 ± 0.434
0.848ValHis: 0.848 ± 0.194
4.342ValIle: 4.342 ± 0.385
3.893ValLys: 3.893 ± 0.451
5.19ValLeu: 5.19 ± 0.582
2.845ValMet: 2.845 ± 0.414
2.994ValAsn: 2.994 ± 0.442
2.994ValPro: 2.994 ± 0.381
1.747ValGln: 1.747 ± 0.281
3.943ValArg: 3.943 ± 0.423
4.042ValSer: 4.042 ± 0.575
5.04ValThr: 5.04 ± 0.528
4.342ValVal: 4.342 ± 0.48
0.749ValTrp: 0.749 ± 0.204
2.495ValTyr: 2.495 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
1.697TrpAla: 1.697 ± 0.233
0.15TrpCys: 0.15 ± 0.098
1.248TrpAsp: 1.248 ± 0.249
1.098TrpGlu: 1.098 ± 0.255
0.599TrpPhe: 0.599 ± 0.162
1.148TrpGly: 1.148 ± 0.231
0.499TrpHis: 0.499 ± 0.189
0.798TrpIle: 0.798 ± 0.217
0.749TrpLys: 0.749 ± 0.246
1.647TrpLeu: 1.647 ± 0.326
0.499TrpMet: 0.499 ± 0.127
0.349TrpAsn: 0.349 ± 0.184
0.499TrpPro: 0.499 ± 0.203
0.749TrpGln: 0.749 ± 0.173
0.998TrpArg: 0.998 ± 0.244
1.048TrpSer: 1.048 ± 0.189
1.397TrpThr: 1.397 ± 0.347
0.998TrpVal: 0.998 ± 0.163
0.25TrpTrp: 0.25 ± 0.122
0.798TrpTyr: 0.798 ± 0.216
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.994TyrAla: 2.994 ± 0.395
0.549TyrCys: 0.549 ± 0.162
2.445TyrAsp: 2.445 ± 0.383
1.996TyrGlu: 1.996 ± 0.303
0.998TyrPhe: 0.998 ± 0.259
2.196TyrGly: 2.196 ± 0.338
0.649TyrHis: 0.649 ± 0.136
1.647TyrIle: 1.647 ± 0.24
1.447TyrLys: 1.447 ± 0.282
3.044TyrLeu: 3.044 ± 0.412
0.649TyrMet: 0.649 ± 0.173
1.547TyrAsn: 1.547 ± 0.245
1.647TyrPro: 1.647 ± 0.294
1.896TyrGln: 1.896 ± 0.297
1.946TyrArg: 1.946 ± 0.357
1.996TyrSer: 1.996 ± 0.296
2.545TyrThr: 2.545 ± 0.306
1.996TyrVal: 1.996 ± 0.301
0.699TyrTrp: 0.699 ± 0.212
1.298TyrTyr: 1.298 ± 0.241
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (20039 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski