Amino acid dipepetide frequency for Staphylococcus phage phiMR11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.519AlaAla: 0.519 ± 0.197
0.297AlaCys: 0.297 ± 0.131
2.225AlaAsp: 2.225 ± 0.349
3.633AlaGlu: 3.633 ± 0.438
2.669AlaPhe: 2.669 ± 0.518
3.856AlaGly: 3.856 ± 0.821
1.186AlaHis: 1.186 ± 0.292
5.339AlaIle: 5.339 ± 0.756
6.599AlaLys: 6.599 ± 0.721
4.597AlaLeu: 4.597 ± 0.735
1.483AlaMet: 1.483 ± 0.502
3.411AlaAsn: 3.411 ± 0.422
2.002AlaPro: 2.002 ± 0.384
3.263AlaGln: 3.263 ± 0.452
2.595AlaArg: 2.595 ± 0.388
4.449AlaSer: 4.449 ± 0.692
4.375AlaThr: 4.375 ± 0.614
3.263AlaVal: 3.263 ± 0.767
0.89AlaTrp: 0.89 ± 0.316
2.373AlaTyr: 2.373 ± 0.44
0.0AlaXaa: 0.0 ± 0.0
Cys
0.148CysAla: 0.148 ± 0.1
0.074CysCys: 0.074 ± 0.082
0.297CysAsp: 0.297 ± 0.138
0.0CysGlu: 0.0 ± 0.0
0.371CysPhe: 0.371 ± 0.16
0.297CysGly: 0.297 ± 0.139
0.0CysHis: 0.0 ± 0.0
0.222CysIle: 0.222 ± 0.104
0.445CysLys: 0.445 ± 0.166
0.297CysLeu: 0.297 ± 0.147
0.074CysMet: 0.074 ± 0.069
0.742CysAsn: 0.742 ± 0.226
0.371CysPro: 0.371 ± 0.187
0.148CysGln: 0.148 ± 0.107
0.297CysArg: 0.297 ± 0.138
0.445CysSer: 0.445 ± 0.226
0.074CysThr: 0.074 ± 0.078
0.297CysVal: 0.297 ± 0.162
0.148CysTrp: 0.148 ± 0.098
0.371CysTyr: 0.371 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
4.227AspAla: 4.227 ± 0.616
0.297AspCys: 0.297 ± 0.158
4.746AspAsp: 4.746 ± 0.785
5.191AspGlu: 5.191 ± 0.641
2.966AspPhe: 2.966 ± 0.487
4.301AspGly: 4.301 ± 0.571
0.371AspHis: 0.371 ± 0.149
4.968AspIle: 4.968 ± 0.692
6.08AspLys: 6.08 ± 0.804
5.265AspLeu: 5.265 ± 0.621
1.557AspMet: 1.557 ± 0.345
3.559AspAsn: 3.559 ± 0.618
1.261AspPro: 1.261 ± 0.263
0.964AspGln: 0.964 ± 0.247
2.076AspArg: 2.076 ± 0.369
3.782AspSer: 3.782 ± 0.538
3.559AspThr: 3.559 ± 0.452
4.523AspVal: 4.523 ± 0.655
0.593AspTrp: 0.593 ± 0.21
3.559AspTyr: 3.559 ± 0.481
0.0AspXaa: 0.0 ± 0.0
Glu
4.375GluAla: 4.375 ± 0.533
0.593GluCys: 0.593 ± 0.203
3.93GluAsp: 3.93 ± 0.732
5.858GluGlu: 5.858 ± 0.821
2.299GluPhe: 2.299 ± 0.423
2.818GluGly: 2.818 ± 0.469
1.631GluHis: 1.631 ± 0.345
6.155GluIle: 6.155 ± 0.823
5.265GluLys: 5.265 ± 0.609
7.786GluLeu: 7.786 ± 0.883
2.521GluMet: 2.521 ± 0.509
4.449GluAsn: 4.449 ± 0.601
2.002GluPro: 2.002 ± 0.34
3.633GluGln: 3.633 ± 0.526
3.337GluArg: 3.337 ± 0.633
3.559GluSer: 3.559 ± 0.537
4.449GluThr: 4.449 ± 0.604
5.191GluVal: 5.191 ± 0.532
1.335GluTrp: 1.335 ± 0.302
3.411GluTyr: 3.411 ± 0.564
0.0GluXaa: 0.0 ± 0.0
Phe
1.928PheAla: 1.928 ± 0.408
0.222PheCys: 0.222 ± 0.123
3.559PheAsp: 3.559 ± 0.444
3.633PheGlu: 3.633 ± 0.531
1.261PhePhe: 1.261 ± 0.346
2.373PheGly: 2.373 ± 0.795
0.593PheHis: 0.593 ± 0.228
3.559PheIle: 3.559 ± 0.519
4.227PheLys: 4.227 ± 0.508
2.373PheLeu: 2.373 ± 0.377
0.667PheMet: 0.667 ± 0.23
2.966PheAsn: 2.966 ± 0.396
0.667PhePro: 0.667 ± 0.286
0.667PheGln: 0.667 ± 0.277
1.335PheArg: 1.335 ± 0.268
2.15PheSer: 2.15 ± 0.365
3.411PheThr: 3.411 ± 0.516
2.521PheVal: 2.521 ± 0.394
0.519PheTrp: 0.519 ± 0.242
1.928PheTyr: 1.928 ± 0.44
0.0PheXaa: 0.0 ± 0.0
Gly
4.968GlyAla: 4.968 ± 0.626
0.371GlyCys: 0.371 ± 0.172
3.93GlyAsp: 3.93 ± 0.606
2.595GlyGlu: 2.595 ± 0.414
2.892GlyPhe: 2.892 ± 0.495
3.188GlyGly: 3.188 ± 0.514
1.631GlyHis: 1.631 ± 0.424
5.042GlyIle: 5.042 ± 0.482
4.449GlyLys: 4.449 ± 0.467
4.375GlyLeu: 4.375 ± 0.688
1.631GlyMet: 1.631 ± 0.289
3.188GlyAsn: 3.188 ± 0.487
0.445GlyPro: 0.445 ± 0.198
2.818GlyGln: 2.818 ± 0.378
2.299GlyArg: 2.299 ± 0.407
3.04GlySer: 3.04 ± 0.469
3.856GlyThr: 3.856 ± 0.53
5.042GlyVal: 5.042 ± 0.918
1.186GlyTrp: 1.186 ± 0.443
3.263GlyTyr: 3.263 ± 0.495
0.0GlyXaa: 0.0 ± 0.0
His
1.261HisAla: 1.261 ± 0.257
0.074HisCys: 0.074 ± 0.078
0.593HisAsp: 0.593 ± 0.196
1.186HisGlu: 1.186 ± 0.315
0.667HisPhe: 0.667 ± 0.229
1.261HisGly: 1.261 ± 0.262
0.297HisHis: 0.297 ± 0.143
1.038HisIle: 1.038 ± 0.236
1.335HisLys: 1.335 ± 0.266
1.186HisLeu: 1.186 ± 0.313
0.222HisMet: 0.222 ± 0.125
1.335HisAsn: 1.335 ± 0.361
0.964HisPro: 0.964 ± 0.274
0.816HisGln: 0.816 ± 0.254
0.445HisArg: 0.445 ± 0.154
1.038HisSer: 1.038 ± 0.287
1.335HisThr: 1.335 ± 0.358
1.186HisVal: 1.186 ± 0.296
0.0HisTrp: 0.0 ± 0.0
1.112HisTyr: 1.112 ± 0.418
0.0HisXaa: 0.0 ± 0.0
Ile
4.894IleAla: 4.894 ± 0.713
0.148IleCys: 0.148 ± 0.103
5.561IleAsp: 5.561 ± 0.66
6.748IleGlu: 6.748 ± 0.882
2.744IlePhe: 2.744 ± 0.521
4.82IleGly: 4.82 ± 0.773
1.112IleHis: 1.112 ± 0.314
4.672IleIle: 4.672 ± 0.568
9.121IleLys: 9.121 ± 0.849
3.411IleLeu: 3.411 ± 0.482
2.225IleMet: 2.225 ± 0.389
4.004IleAsn: 4.004 ± 0.585
2.744IlePro: 2.744 ± 0.445
3.04IleGln: 3.04 ± 0.496
3.263IleArg: 3.263 ± 0.537
3.708IleSer: 3.708 ± 0.517
4.968IleThr: 4.968 ± 0.583
4.301IleVal: 4.301 ± 0.56
0.816IleTrp: 0.816 ± 0.325
2.521IleTyr: 2.521 ± 0.565
0.0IleXaa: 0.0 ± 0.0
Lys
4.968LysAla: 4.968 ± 0.5
0.222LysCys: 0.222 ± 0.125
5.561LysAsp: 5.561 ± 0.726
8.75LysGlu: 8.75 ± 1.175
2.669LysPhe: 2.669 ± 0.432
5.191LysGly: 5.191 ± 0.773
1.557LysHis: 1.557 ± 0.3
6.451LysIle: 6.451 ± 0.719
7.563LysLys: 7.563 ± 0.842
8.082LysLeu: 8.082 ± 0.853
2.521LysMet: 2.521 ± 0.454
5.413LysAsn: 5.413 ± 0.735
2.521LysPro: 2.521 ± 0.456
4.449LysGln: 4.449 ± 0.549
4.746LysArg: 4.746 ± 0.535
4.449LysSer: 4.449 ± 0.568
5.932LysThr: 5.932 ± 0.674
5.561LysVal: 5.561 ± 0.63
0.89LysTrp: 0.89 ± 0.249
3.263LysTyr: 3.263 ± 0.481
0.0LysXaa: 0.0 ± 0.0
Leu
3.856LeuAla: 3.856 ± 0.649
0.445LeuCys: 0.445 ± 0.204
5.339LeuAsp: 5.339 ± 0.503
5.858LeuGlu: 5.858 ± 1.002
3.485LeuPhe: 3.485 ± 0.541
3.782LeuGly: 3.782 ± 0.509
1.261LeuHis: 1.261 ± 0.351
4.746LeuIle: 4.746 ± 0.557
7.118LeuLys: 7.118 ± 0.9
5.487LeuLeu: 5.487 ± 0.707
1.631LeuMet: 1.631 ± 0.492
5.487LeuAsn: 5.487 ± 0.596
2.299LeuPro: 2.299 ± 0.459
2.669LeuGln: 2.669 ± 0.322
3.188LeuArg: 3.188 ± 0.604
5.042LeuSer: 5.042 ± 0.484
5.413LeuThr: 5.413 ± 0.698
4.078LeuVal: 4.078 ± 0.512
0.519LeuTrp: 0.519 ± 0.248
3.782LeuTyr: 3.782 ± 0.611
0.0LeuXaa: 0.0 ± 0.0
Met
1.705MetAla: 1.705 ± 0.48
0.074MetCys: 0.074 ± 0.063
1.261MetAsp: 1.261 ± 0.382
1.335MetGlu: 1.335 ± 0.292
1.038MetPhe: 1.038 ± 0.277
1.409MetGly: 1.409 ± 0.323
0.445MetHis: 0.445 ± 0.194
1.409MetIle: 1.409 ± 0.293
2.002MetLys: 2.002 ± 0.37
2.447MetLeu: 2.447 ± 0.431
1.186MetMet: 1.186 ± 0.279
1.631MetAsn: 1.631 ± 0.369
1.186MetPro: 1.186 ± 0.277
1.928MetGln: 1.928 ± 0.537
0.816MetArg: 0.816 ± 0.238
1.705MetSer: 1.705 ± 0.389
1.928MetThr: 1.928 ± 0.435
1.038MetVal: 1.038 ± 0.249
0.445MetTrp: 0.445 ± 0.149
1.038MetTyr: 1.038 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
4.523AsnAla: 4.523 ± 0.651
0.519AsnCys: 0.519 ± 0.179
5.116AsnAsp: 5.116 ± 0.672
5.116AsnGlu: 5.116 ± 0.592
2.818AsnPhe: 2.818 ± 0.474
4.597AsnGly: 4.597 ± 0.634
0.742AsnHis: 0.742 ± 0.263
4.152AsnIle: 4.152 ± 0.756
6.08AsnLys: 6.08 ± 0.846
4.004AsnLeu: 4.004 ± 0.57
1.409AsnMet: 1.409 ± 0.295
5.265AsnAsn: 5.265 ± 1.027
2.595AsnPro: 2.595 ± 0.489
2.373AsnGln: 2.373 ± 0.468
2.15AsnArg: 2.15 ± 0.364
3.559AsnSer: 3.559 ± 0.45
3.337AsnThr: 3.337 ± 0.486
3.188AsnVal: 3.188 ± 0.585
0.89AsnTrp: 0.89 ± 0.26
2.521AsnTyr: 2.521 ± 0.412
0.0AsnXaa: 0.0 ± 0.0
Pro
1.261ProAla: 1.261 ± 0.268
0.074ProCys: 0.074 ± 0.069
1.631ProAsp: 1.631 ± 0.332
1.928ProGlu: 1.928 ± 0.449
1.409ProPhe: 1.409 ± 0.329
1.928ProGly: 1.928 ± 0.514
0.519ProHis: 0.519 ± 0.2
2.373ProIle: 2.373 ± 0.486
2.744ProLys: 2.744 ± 0.473
1.557ProLeu: 1.557 ± 0.3
0.816ProMet: 0.816 ± 0.254
2.002ProAsn: 2.002 ± 0.495
0.593ProPro: 0.593 ± 0.28
1.261ProGln: 1.261 ± 0.299
1.261ProArg: 1.261 ± 0.307
2.002ProSer: 2.002 ± 0.393
1.78ProThr: 1.78 ± 0.299
1.705ProVal: 1.705 ± 0.381
0.074ProTrp: 0.074 ± 0.083
1.335ProTyr: 1.335 ± 0.327
0.0ProXaa: 0.0 ± 0.0
Gln
4.078GlnAla: 4.078 ± 0.481
0.371GlnCys: 0.371 ± 0.195
1.854GlnAsp: 1.854 ± 0.356
2.892GlnGlu: 2.892 ± 0.484
2.076GlnPhe: 2.076 ± 0.366
2.744GlnGly: 2.744 ± 0.4
1.261GlnHis: 1.261 ± 0.249
2.818GlnIle: 2.818 ± 0.368
2.521GlnLys: 2.521 ± 0.467
2.669GlnLeu: 2.669 ± 0.444
1.705GlnMet: 1.705 ± 0.421
3.04GlnAsn: 3.04 ± 0.494
1.928GlnPro: 1.928 ± 0.416
2.447GlnGln: 2.447 ± 0.52
1.705GlnArg: 1.705 ± 0.323
1.705GlnSer: 1.705 ± 0.354
1.631GlnThr: 1.631 ± 0.357
3.04GlnVal: 3.04 ± 0.502
0.297GlnTrp: 0.297 ± 0.148
1.186GlnTyr: 1.186 ± 0.323
0.0GlnXaa: 0.0 ± 0.0
Arg
1.557ArgAla: 1.557 ± 0.379
0.371ArgCys: 0.371 ± 0.142
2.966ArgAsp: 2.966 ± 0.452
3.337ArgGlu: 3.337 ± 0.467
2.15ArgPhe: 2.15 ± 0.455
2.299ArgGly: 2.299 ± 0.389
1.261ArgHis: 1.261 ± 0.316
2.447ArgIle: 2.447 ± 0.501
3.559ArgLys: 3.559 ± 0.546
3.782ArgLeu: 3.782 ± 0.581
0.964ArgMet: 0.964 ± 0.236
2.744ArgAsn: 2.744 ± 0.39
0.964ArgPro: 0.964 ± 0.228
1.78ArgGln: 1.78 ± 0.398
1.335ArgArg: 1.335 ± 0.269
1.705ArgSer: 1.705 ± 0.353
1.557ArgThr: 1.557 ± 0.347
2.595ArgVal: 2.595 ± 0.349
0.371ArgTrp: 0.371 ± 0.161
2.15ArgTyr: 2.15 ± 0.369
0.0ArgXaa: 0.0 ± 0.0
Ser
3.708SerAla: 3.708 ± 0.501
0.148SerCys: 0.148 ± 0.148
4.227SerAsp: 4.227 ± 0.55
3.782SerGlu: 3.782 ± 0.466
2.447SerPhe: 2.447 ± 0.551
4.375SerGly: 4.375 ± 0.648
0.593SerHis: 0.593 ± 0.168
5.191SerIle: 5.191 ± 0.704
5.784SerLys: 5.784 ± 0.621
2.892SerLeu: 2.892 ± 0.454
1.631SerMet: 1.631 ± 0.315
4.301SerAsn: 4.301 ± 0.524
0.964SerPro: 0.964 ± 0.296
2.521SerGln: 2.521 ± 0.495
2.002SerArg: 2.002 ± 0.304
3.263SerSer: 3.263 ± 0.561
3.485SerThr: 3.485 ± 0.467
3.559SerVal: 3.559 ± 0.609
0.667SerTrp: 0.667 ± 0.195
2.002SerTyr: 2.002 ± 0.343
0.0SerXaa: 0.0 ± 0.0
Thr
4.004ThrAla: 4.004 ± 0.642
0.074ThrCys: 0.074 ± 0.069
4.004ThrAsp: 4.004 ± 0.512
3.782ThrGlu: 3.782 ± 0.496
2.744ThrPhe: 2.744 ± 0.534
4.375ThrGly: 4.375 ± 0.681
1.631ThrHis: 1.631 ± 0.37
4.597ThrIle: 4.597 ± 0.811
4.894ThrLys: 4.894 ± 0.639
5.784ThrLeu: 5.784 ± 0.53
0.816ThrMet: 0.816 ± 0.251
4.523ThrAsn: 4.523 ± 0.706
1.557ThrPro: 1.557 ± 0.329
2.447ThrGln: 2.447 ± 0.574
2.225ThrArg: 2.225 ± 0.448
4.968ThrSer: 4.968 ± 0.845
3.633ThrThr: 3.633 ± 0.621
3.114ThrVal: 3.114 ± 0.481
0.742ThrTrp: 0.742 ± 0.293
2.447ThrTyr: 2.447 ± 0.454
0.0ThrXaa: 0.0 ± 0.0
Val
4.672ValAla: 4.672 ± 0.78
0.148ValCys: 0.148 ± 0.107
4.894ValAsp: 4.894 ± 0.745
4.449ValGlu: 4.449 ± 0.585
2.15ValPhe: 2.15 ± 0.408
3.114ValGly: 3.114 ± 0.487
0.371ValHis: 0.371 ± 0.16
5.042ValIle: 5.042 ± 0.653
5.858ValLys: 5.858 ± 0.638
5.339ValLeu: 5.339 ± 0.6
1.854ValMet: 1.854 ± 0.35
3.263ValAsn: 3.263 ± 0.479
1.928ValPro: 1.928 ± 0.36
1.557ValGln: 1.557 ± 0.349
2.521ValArg: 2.521 ± 0.381
3.856ValSer: 3.856 ± 0.672
4.078ValThr: 4.078 ± 0.656
3.411ValVal: 3.411 ± 0.557
1.038ValTrp: 1.038 ± 0.256
2.447ValTyr: 2.447 ± 0.437
0.0ValXaa: 0.0 ± 0.0
Trp
0.742TrpAla: 0.742 ± 0.245
0.074TrpCys: 0.074 ± 0.068
0.445TrpAsp: 0.445 ± 0.151
1.038TrpGlu: 1.038 ± 0.264
0.519TrpPhe: 0.519 ± 0.162
0.89TrpGly: 0.89 ± 0.296
0.222TrpHis: 0.222 ± 0.117
0.816TrpIle: 0.816 ± 0.299
1.038TrpLys: 1.038 ± 0.271
1.038TrpLeu: 1.038 ± 0.291
0.074TrpMet: 0.074 ± 0.073
0.89TrpAsn: 0.89 ± 0.261
0.0TrpPro: 0.0 ± 0.0
0.816TrpGln: 0.816 ± 0.242
0.445TrpArg: 0.445 ± 0.189
0.742TrpSer: 0.742 ± 0.25
1.038TrpThr: 1.038 ± 0.218
0.964TrpVal: 0.964 ± 0.267
0.0TrpTrp: 0.0 ± 0.0
0.371TrpTyr: 0.371 ± 0.183
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.631TyrAla: 1.631 ± 0.343
0.519TyrCys: 0.519 ± 0.167
1.705TyrAsp: 1.705 ± 0.47
3.485TyrGlu: 3.485 ± 0.596
1.186TyrPhe: 1.186 ± 0.287
2.595TyrGly: 2.595 ± 0.527
0.667TyrHis: 0.667 ± 0.247
3.782TyrIle: 3.782 ± 0.538
4.078TyrLys: 4.078 ± 0.638
3.114TyrLeu: 3.114 ± 0.48
0.964TyrMet: 0.964 ± 0.295
2.744TyrAsn: 2.744 ± 0.474
1.261TyrPro: 1.261 ± 0.353
2.373TyrGln: 2.373 ± 0.346
1.854TyrArg: 1.854 ± 0.439
2.447TyrSer: 2.447 ± 0.451
2.521TyrThr: 2.521 ± 0.484
3.411TyrVal: 3.411 ± 0.493
0.742TyrTrp: 0.742 ± 0.208
2.002TyrTyr: 2.002 ± 0.464
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (13487 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski