Amino acid dipepetide frequency for Burkholderia phage KS5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.371AlaAla: 15.371 ± 1.884
0.64AlaCys: 0.64 ± 0.224
8.783AlaAsp: 8.783 ± 1.144
6.221AlaGlu: 6.221 ± 0.708
3.202AlaPhe: 3.202 ± 0.639
11.528AlaGly: 11.528 ± 0.938
2.196AlaHis: 2.196 ± 0.472
5.489AlaIle: 5.489 ± 0.704
5.489AlaLys: 5.489 ± 0.702
12.534AlaLeu: 12.534 ± 1.317
2.836AlaMet: 2.836 ± 0.46
3.385AlaAsn: 3.385 ± 0.468
4.483AlaPro: 4.483 ± 0.635
4.666AlaGln: 4.666 ± 0.573
9.058AlaArg: 9.058 ± 0.933
6.587AlaSer: 6.587 ± 0.915
7.96AlaThr: 7.96 ± 0.886
6.679AlaVal: 6.679 ± 0.72
2.104AlaTrp: 2.104 ± 0.427
3.477AlaTyr: 3.477 ± 0.682
0.0AlaXaa: 0.0 ± 0.0
Cys
1.189CysAla: 1.189 ± 0.345
0.0CysCys: 0.0 ± 0.0
0.183CysAsp: 0.183 ± 0.124
0.549CysGlu: 0.549 ± 0.21
0.274CysPhe: 0.274 ± 0.133
0.366CysGly: 0.366 ± 0.156
0.183CysHis: 0.183 ± 0.124
0.183CysIle: 0.183 ± 0.114
0.091CysLys: 0.091 ± 0.101
0.823CysLeu: 0.823 ± 0.317
0.549CysMet: 0.549 ± 0.224
0.274CysAsn: 0.274 ± 0.157
0.457CysPro: 0.457 ± 0.196
0.366CysGln: 0.366 ± 0.21
0.549CysArg: 0.549 ± 0.258
0.457CysSer: 0.457 ± 0.174
0.732CysThr: 0.732 ± 0.287
0.64CysVal: 0.64 ± 0.219
0.274CysTrp: 0.274 ± 0.15
0.091CysTyr: 0.091 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
9.515AspAla: 9.515 ± 0.849
0.366AspCys: 0.366 ± 0.179
2.836AspAsp: 2.836 ± 0.484
3.568AspGlu: 3.568 ± 0.586
2.196AspPhe: 2.196 ± 0.43
6.13AspGly: 6.13 ± 0.799
1.281AspHis: 1.281 ± 0.309
2.653AspIle: 2.653 ± 0.513
2.287AspLys: 2.287 ± 0.659
5.306AspLeu: 5.306 ± 0.881
0.823AspMet: 0.823 ± 0.279
1.281AspAsn: 1.281 ± 0.411
3.477AspPro: 3.477 ± 0.642
2.653AspGln: 2.653 ± 0.517
4.758AspArg: 4.758 ± 0.536
1.921AspSer: 1.921 ± 0.395
3.843AspThr: 3.843 ± 0.686
5.947AspVal: 5.947 ± 0.997
0.823AspTrp: 0.823 ± 0.249
1.098AspTyr: 1.098 ± 0.29
0.0AspXaa: 0.0 ± 0.0
Glu
5.306GluAla: 5.306 ± 0.632
0.457GluCys: 0.457 ± 0.187
1.921GluAsp: 1.921 ± 0.461
2.836GluGlu: 2.836 ± 0.553
2.104GluPhe: 2.104 ± 0.47
2.562GluGly: 2.562 ± 0.496
1.189GluHis: 1.189 ± 0.333
3.477GluIle: 3.477 ± 0.698
2.928GluLys: 2.928 ± 0.506
6.13GluLeu: 6.13 ± 0.752
1.738GluMet: 1.738 ± 0.357
1.098GluAsn: 1.098 ± 0.256
3.568GluPro: 3.568 ± 0.672
2.379GluGln: 2.379 ± 0.441
5.764GluArg: 5.764 ± 0.709
3.568GluSer: 3.568 ± 0.738
1.738GluThr: 1.738 ± 0.453
4.026GluVal: 4.026 ± 0.664
0.823GluTrp: 0.823 ± 0.291
1.372GluTyr: 1.372 ± 0.422
0.0GluXaa: 0.0 ± 0.0
Phe
4.117PheAla: 4.117 ± 0.583
0.274PheCys: 0.274 ± 0.142
2.196PheAsp: 2.196 ± 0.41
1.83PheGlu: 1.83 ± 0.345
1.098PhePhe: 1.098 ± 0.354
2.562PheGly: 2.562 ± 0.441
1.006PheHis: 1.006 ± 0.336
1.006PheIle: 1.006 ± 0.284
1.647PheLys: 1.647 ± 0.353
2.196PheLeu: 2.196 ± 0.466
0.64PheMet: 0.64 ± 0.283
1.281PheAsn: 1.281 ± 0.314
1.464PhePro: 1.464 ± 0.388
1.189PheGln: 1.189 ± 0.386
2.287PheArg: 2.287 ± 0.468
2.745PheSer: 2.745 ± 0.523
1.647PheThr: 1.647 ± 0.437
2.47PheVal: 2.47 ± 0.541
0.732PheTrp: 0.732 ± 0.228
0.457PheTyr: 0.457 ± 0.18
0.0PheXaa: 0.0 ± 0.0
Gly
9.332GlyAla: 9.332 ± 1.191
0.915GlyCys: 0.915 ± 0.315
4.666GlyAsp: 4.666 ± 0.629
4.483GlyGlu: 4.483 ± 0.605
3.111GlyPhe: 3.111 ± 0.404
6.404GlyGly: 6.404 ± 0.938
1.921GlyHis: 1.921 ± 0.489
4.666GlyIle: 4.666 ± 0.827
3.477GlyLys: 3.477 ± 0.666
6.404GlyLeu: 6.404 ± 0.898
2.287GlyMet: 2.287 ± 0.445
2.196GlyAsn: 2.196 ± 0.531
2.562GlyPro: 2.562 ± 0.559
1.83GlyGln: 1.83 ± 0.394
5.215GlyArg: 5.215 ± 0.602
2.745GlySer: 2.745 ± 0.5
5.764GlyThr: 5.764 ± 1.126
6.587GlyVal: 6.587 ± 0.805
1.555GlyTrp: 1.555 ± 0.368
1.464GlyTyr: 1.464 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
2.562HisAla: 2.562 ± 0.5
0.091HisCys: 0.091 ± 0.087
1.372HisAsp: 1.372 ± 0.385
1.098HisGlu: 1.098 ± 0.28
0.732HisPhe: 0.732 ± 0.255
2.013HisGly: 2.013 ± 0.48
0.457HisHis: 0.457 ± 0.167
1.006HisIle: 1.006 ± 0.28
0.64HisLys: 0.64 ± 0.216
1.647HisLeu: 1.647 ± 0.424
0.366HisMet: 0.366 ± 0.187
0.549HisAsn: 0.549 ± 0.286
1.281HisPro: 1.281 ± 0.408
0.549HisGln: 0.549 ± 0.212
1.647HisArg: 1.647 ± 0.367
1.006HisSer: 1.006 ± 0.239
1.189HisThr: 1.189 ± 0.366
1.555HisVal: 1.555 ± 0.378
0.274HisTrp: 0.274 ± 0.164
0.366HisTyr: 0.366 ± 0.132
0.0HisXaa: 0.0 ± 0.0
Ile
6.587IleAla: 6.587 ± 0.766
0.457IleCys: 0.457 ± 0.172
4.483IleAsp: 4.483 ± 0.823
3.202IleGlu: 3.202 ± 0.598
0.915IlePhe: 0.915 ± 0.293
5.032IleGly: 5.032 ± 0.844
0.732IleHis: 0.732 ± 0.238
0.549IleIle: 0.549 ± 0.211
1.738IleLys: 1.738 ± 0.5
2.379IleLeu: 2.379 ± 0.476
0.549IleMet: 0.549 ± 0.164
1.098IleAsn: 1.098 ± 0.23
1.281IlePro: 1.281 ± 0.415
2.104IleGln: 2.104 ± 0.566
2.836IleArg: 2.836 ± 0.408
3.202IleSer: 3.202 ± 0.58
3.111IleThr: 3.111 ± 0.572
3.568IleVal: 3.568 ± 0.586
0.183IleTrp: 0.183 ± 0.105
1.006IleTyr: 1.006 ± 0.361
0.0IleXaa: 0.0 ± 0.0
Lys
4.941LysAla: 4.941 ± 0.652
0.366LysCys: 0.366 ± 0.198
1.555LysAsp: 1.555 ± 0.377
2.287LysGlu: 2.287 ± 0.42
1.647LysPhe: 1.647 ± 0.285
3.111LysGly: 3.111 ± 0.504
0.457LysHis: 0.457 ± 0.277
1.464LysIle: 1.464 ± 0.347
2.836LysLys: 2.836 ± 0.53
3.477LysLeu: 3.477 ± 0.718
1.098LysMet: 1.098 ± 0.303
1.555LysAsn: 1.555 ± 0.346
2.013LysPro: 2.013 ± 0.36
1.83LysGln: 1.83 ± 0.515
4.483LysArg: 4.483 ± 0.54
2.013LysSer: 2.013 ± 0.401
2.928LysThr: 2.928 ± 0.353
3.202LysVal: 3.202 ± 0.52
0.457LysTrp: 0.457 ± 0.19
0.915LysTyr: 0.915 ± 0.279
0.0LysXaa: 0.0 ± 0.0
Leu
11.619LeuAla: 11.619 ± 1.084
1.464LeuCys: 1.464 ± 0.431
6.77LeuAsp: 6.77 ± 0.638
5.581LeuGlu: 5.581 ± 0.563
3.66LeuPhe: 3.66 ± 0.619
6.496LeuGly: 6.496 ± 1.063
1.555LeuHis: 1.555 ± 0.399
3.568LeuIle: 3.568 ± 0.442
4.392LeuLys: 4.392 ± 0.666
8.143LeuLeu: 8.143 ± 0.933
2.104LeuMet: 2.104 ± 0.48
2.836LeuAsn: 2.836 ± 0.425
5.124LeuPro: 5.124 ± 0.808
3.202LeuGln: 3.202 ± 0.434
7.411LeuArg: 7.411 ± 0.655
5.672LeuSer: 5.672 ± 0.779
5.215LeuThr: 5.215 ± 0.66
6.313LeuVal: 6.313 ± 0.699
0.457LeuTrp: 0.457 ± 0.221
2.104LeuTyr: 2.104 ± 0.538
0.0LeuXaa: 0.0 ± 0.0
Met
2.379MetAla: 2.379 ± 0.439
0.0MetCys: 0.0 ± 0.0
0.823MetAsp: 0.823 ± 0.323
1.098MetGlu: 1.098 ± 0.363
0.823MetPhe: 0.823 ± 0.275
1.006MetGly: 1.006 ± 0.302
0.549MetHis: 0.549 ± 0.276
0.732MetIle: 0.732 ± 0.284
1.281MetLys: 1.281 ± 0.33
2.196MetLeu: 2.196 ± 0.368
0.366MetMet: 0.366 ± 0.248
0.823MetAsn: 0.823 ± 0.273
0.915MetPro: 0.915 ± 0.266
1.372MetGln: 1.372 ± 0.364
2.379MetArg: 2.379 ± 0.573
1.189MetSer: 1.189 ± 0.401
2.379MetThr: 2.379 ± 0.382
1.006MetVal: 1.006 ± 0.277
0.274MetTrp: 0.274 ± 0.167
0.366MetTyr: 0.366 ± 0.207
0.0MetXaa: 0.0 ± 0.0
Asn
2.836AsnAla: 2.836 ± 0.473
0.091AsnCys: 0.091 ± 0.079
1.83AsnAsp: 1.83 ± 0.396
1.647AsnGlu: 1.647 ± 0.31
1.098AsnPhe: 1.098 ± 0.305
3.202AsnGly: 3.202 ± 0.525
0.366AsnHis: 0.366 ± 0.132
1.006AsnIle: 1.006 ± 0.304
0.732AsnLys: 0.732 ± 0.28
2.196AsnLeu: 2.196 ± 0.521
0.457AsnMet: 0.457 ± 0.242
0.823AsnAsn: 0.823 ± 0.322
1.738AsnPro: 1.738 ± 0.42
1.006AsnGln: 1.006 ± 0.307
3.019AsnArg: 3.019 ± 0.493
1.372AsnSer: 1.372 ± 0.385
1.281AsnThr: 1.281 ± 0.362
2.562AsnVal: 2.562 ± 0.363
0.64AsnTrp: 0.64 ± 0.196
0.64AsnTyr: 0.64 ± 0.264
0.0AsnXaa: 0.0 ± 0.0
Pro
6.404ProAla: 6.404 ± 0.84
0.274ProCys: 0.274 ± 0.155
4.026ProAsp: 4.026 ± 0.816
2.562ProGlu: 2.562 ± 0.546
1.555ProPhe: 1.555 ± 0.343
2.196ProGly: 2.196 ± 0.331
0.732ProHis: 0.732 ± 0.34
3.111ProIle: 3.111 ± 0.478
1.555ProLys: 1.555 ± 0.322
4.575ProLeu: 4.575 ± 0.828
0.64ProMet: 0.64 ± 0.219
1.464ProAsn: 1.464 ± 0.306
2.836ProPro: 2.836 ± 0.564
0.732ProGln: 0.732 ± 0.232
3.843ProArg: 3.843 ± 0.639
3.385ProSer: 3.385 ± 0.535
2.104ProThr: 2.104 ± 0.392
2.745ProVal: 2.745 ± 0.565
0.549ProTrp: 0.549 ± 0.2
1.189ProTyr: 1.189 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
3.477GlnAla: 3.477 ± 0.451
0.549GlnCys: 0.549 ± 0.221
2.013GlnAsp: 2.013 ± 0.383
1.555GlnGlu: 1.555 ± 0.351
1.098GlnPhe: 1.098 ± 0.284
2.013GlnGly: 2.013 ± 0.524
1.189GlnHis: 1.189 ± 0.283
1.189GlnIle: 1.189 ± 0.518
1.372GlnLys: 1.372 ± 0.416
4.483GlnLeu: 4.483 ± 0.594
0.732GlnMet: 0.732 ± 0.34
0.915GlnAsn: 0.915 ± 0.246
1.006GlnPro: 1.006 ± 0.309
2.196GlnGln: 2.196 ± 0.59
3.385GlnArg: 3.385 ± 0.49
2.745GlnSer: 2.745 ± 0.462
2.196GlnThr: 2.196 ± 0.375
2.196GlnVal: 2.196 ± 0.468
0.549GlnTrp: 0.549 ± 0.306
1.83GlnTyr: 1.83 ± 0.34
0.0GlnXaa: 0.0 ± 0.0
Arg
11.894ArgAla: 11.894 ± 1.149
0.457ArgCys: 0.457 ± 0.219
4.849ArgAsp: 4.849 ± 0.598
4.392ArgGlu: 4.392 ± 0.633
1.83ArgPhe: 1.83 ± 0.313
6.496ArgGly: 6.496 ± 1.125
1.647ArgHis: 1.647 ± 0.446
4.209ArgIle: 4.209 ± 0.612
2.562ArgLys: 2.562 ± 0.616
8.051ArgLeu: 8.051 ± 0.632
1.281ArgMet: 1.281 ± 0.442
2.562ArgAsn: 2.562 ± 0.555
3.111ArgPro: 3.111 ± 0.458
3.477ArgGln: 3.477 ± 0.554
7.045ArgArg: 7.045 ± 1.018
3.66ArgSer: 3.66 ± 0.523
4.758ArgThr: 4.758 ± 0.642
5.855ArgVal: 5.855 ± 0.717
0.732ArgTrp: 0.732 ± 0.242
2.287ArgTyr: 2.287 ± 0.384
0.0ArgXaa: 0.0 ± 0.0
Ser
7.319SerAla: 7.319 ± 0.693
0.549SerCys: 0.549 ± 0.163
3.477SerAsp: 3.477 ± 0.624
1.921SerGlu: 1.921 ± 0.398
1.189SerPhe: 1.189 ± 0.311
4.026SerGly: 4.026 ± 0.732
1.464SerHis: 1.464 ± 0.404
2.653SerIle: 2.653 ± 0.527
2.562SerLys: 2.562 ± 0.573
5.581SerLeu: 5.581 ± 0.682
1.098SerMet: 1.098 ± 0.302
1.647SerAsn: 1.647 ± 0.375
3.019SerPro: 3.019 ± 0.682
2.013SerGln: 2.013 ± 0.447
4.209SerArg: 4.209 ± 0.527
2.745SerSer: 2.745 ± 0.535
2.836SerThr: 2.836 ± 0.417
3.111SerVal: 3.111 ± 0.484
0.64SerTrp: 0.64 ± 0.241
1.189SerTyr: 1.189 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
5.489ThrAla: 5.489 ± 0.748
0.366ThrCys: 0.366 ± 0.169
3.477ThrAsp: 3.477 ± 0.679
3.568ThrGlu: 3.568 ± 0.639
2.104ThrPhe: 2.104 ± 0.584
5.947ThrGly: 5.947 ± 0.832
0.915ThrHis: 0.915 ± 0.297
2.47ThrIle: 2.47 ± 0.409
2.196ThrLys: 2.196 ± 0.455
6.313ThrLeu: 6.313 ± 0.568
1.647ThrMet: 1.647 ± 0.352
1.647ThrAsn: 1.647 ± 0.351
3.294ThrPro: 3.294 ± 0.616
1.464ThrGln: 1.464 ± 0.352
4.026ThrArg: 4.026 ± 0.493
3.568ThrSer: 3.568 ± 0.665
5.215ThrThr: 5.215 ± 0.786
5.398ThrVal: 5.398 ± 0.78
1.189ThrTrp: 1.189 ± 0.273
1.464ThrTyr: 1.464 ± 0.413
0.0ThrXaa: 0.0 ± 0.0
Val
8.234ValAla: 8.234 ± 0.88
0.457ValCys: 0.457 ± 0.209
5.581ValAsp: 5.581 ± 0.508
4.849ValGlu: 4.849 ± 0.59
2.47ValPhe: 2.47 ± 0.413
4.026ValGly: 4.026 ± 0.571
1.098ValHis: 1.098 ± 0.305
3.751ValIle: 3.751 ± 0.469
3.294ValLys: 3.294 ± 0.451
6.862ValLeu: 6.862 ± 1.062
2.013ValMet: 2.013 ± 0.472
2.013ValAsn: 2.013 ± 0.5
3.019ValPro: 3.019 ± 0.456
2.287ValGln: 2.287 ± 0.322
5.672ValArg: 5.672 ± 0.738
2.379ValSer: 2.379 ± 0.409
5.032ValThr: 5.032 ± 0.9
4.3ValVal: 4.3 ± 0.58
1.006ValTrp: 1.006 ± 0.368
2.287ValTyr: 2.287 ± 0.555
0.0ValXaa: 0.0 ± 0.0
Trp
1.555TrpAla: 1.555 ± 0.372
0.091TrpCys: 0.091 ± 0.096
0.915TrpAsp: 0.915 ± 0.281
0.64TrpGlu: 0.64 ± 0.189
0.549TrpPhe: 0.549 ± 0.181
0.549TrpGly: 0.549 ± 0.22
0.732TrpHis: 0.732 ± 0.272
0.549TrpIle: 0.549 ± 0.21
0.457TrpLys: 0.457 ± 0.167
1.555TrpLeu: 1.555 ± 0.372
0.183TrpMet: 0.183 ± 0.139
0.64TrpAsn: 0.64 ± 0.193
0.64TrpPro: 0.64 ± 0.306
0.366TrpGln: 0.366 ± 0.147
1.647TrpArg: 1.647 ± 0.36
0.732TrpSer: 0.732 ± 0.252
0.732TrpThr: 0.732 ± 0.231
0.64TrpVal: 0.64 ± 0.188
0.0TrpTrp: 0.0 ± 0.0
0.64TrpTyr: 0.64 ± 0.283
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.562TyrAla: 2.562 ± 0.497
0.366TyrCys: 0.366 ± 0.165
1.006TyrAsp: 1.006 ± 0.274
1.098TyrGlu: 1.098 ± 0.393
1.189TyrPhe: 1.189 ± 0.47
1.921TyrGly: 1.921 ± 0.306
0.732TyrHis: 0.732 ± 0.229
1.281TyrIle: 1.281 ± 0.333
1.189TyrLys: 1.189 ± 0.292
2.836TyrLeu: 2.836 ± 0.539
0.457TyrMet: 0.457 ± 0.189
0.457TyrAsn: 0.457 ± 0.204
1.189TyrPro: 1.189 ± 0.267
1.006TyrGln: 1.006 ± 0.268
1.921TyrArg: 1.921 ± 0.462
1.555TyrSer: 1.555 ± 0.351
1.098TyrThr: 1.098 ± 0.3
1.83TyrVal: 1.83 ± 0.372
0.549TyrTrp: 0.549 ± 0.177
0.64TyrTyr: 0.64 ± 0.221
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (10931 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski