Candidatus Rhodobacter lobularis
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4763 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0J9E1N7|A0A0J9E1N7_9RHOB Sarcosine oxidase beta subunit OS=Candidatus Rhodobacter lobularis OX=1675527 GN=AIOL_001767 PE=4 SV=1
MM1 pKa = 7.82 SIFSKK6 pKa = 10.83 LNRR9 pKa = 11.84 ALSAIFFVAVAAAGSPSWAQPAFTVGFTPNSIAPSGTTRR48 pKa = 11.84 MIFTIDD54 pKa = 3.46 NSSGGAVTSMDD65 pKa = 3.65 FTNALPAGITVAVAPNAFTSCGGASASSATVTAMAGGSSIAMADD109 pKa = 3.62 GQLAPGATCTVEE121 pKa = 4.45 ANVTATATATNGPATLNTSNGSSASASVGLTVDD154 pKa = 3.8 AAATAVGKK162 pKa = 10.54 AFAPTTIGIGGVSRR176 pKa = 11.84 LTLTVDD182 pKa = 3.55 NSAGANAQNFTLTDD196 pKa = 3.64 NLPAGMVLADD206 pKa = 4.47 PPNTATTCPVAQSQTLTGVGGGSILEE232 pKa = 4.16 LQSTGGLVFTPSFMVVNALPAGTVCTVSADD262 pKa = 3.55 VTASVPGSLQNVTEE276 pKa = 4.34 PFLDD280 pKa = 3.91 SGSAAATLTVTGAPAGAPQLTKK302 pKa = 10.77 EE303 pKa = 4.24 FTDD306 pKa = 4.14 DD307 pKa = 3.28 PVAAGGSATLEE318 pKa = 3.78 FEE320 pKa = 3.78 ILNRR324 pKa = 11.84 SRR326 pKa = 11.84 SDD328 pKa = 3.08 AASNIAFTDD337 pKa = 3.53 DD338 pKa = 2.98 MSALFGALAGTVTAIGTNTCGGTPSGVGTSSLSYY372 pKa = 10.75 SGGTVGAGLRR382 pKa = 11.84 CNITVTISVPAGVSTGSYY400 pKa = 9.24 TNTSTAVTADD410 pKa = 3.48 VGGMSLTGNAATANLSVLAALDD432 pKa = 4.11 LPPVLSFTTPDD443 pKa = 3.23 GNAGGVSTWSFTLSNAASSALTDD466 pKa = 3.42 GTFDD470 pKa = 3.49 VQMNPPLPFPTNASLPPMPDD490 pKa = 3.29 PPCGVGSSLSLAFLDD505 pKa = 4.1 TDD507 pKa = 3.68 QQALRR512 pKa = 11.84 LTGASLATSGAAGDD526 pKa = 3.88 SCTFDD531 pKa = 3.27 VDD533 pKa = 3.37 IGLNSDD539 pKa = 5.14 LPPLQYY545 pKa = 11.04 NFTSGAPSGTVNGATRR561 pKa = 11.84 TGTPASSSMTVGTNLNLSFAKK582 pKa = 10.45 SFLATAVPGGTVDD595 pKa = 3.94 LEE597 pKa = 4.28 FVITSSGDD605 pKa = 3.46 STDD608 pKa = 2.91 ATAISFTDD616 pKa = 5.0 DD617 pKa = 3.09 INAMLAGTSLNAVISNEE634 pKa = 4.01 CGGNLTGTTNLSYY647 pKa = 10.57 TGGSLTGGTSCAIVTRR663 pKa = 11.84 LDD665 pKa = 3.43 VPAAAAIGTYY675 pKa = 10.32 TNTTSALSATPSGSSTVEE693 pKa = 3.91 TFGTASADD701 pKa = 3.47 LTVAGLTFEE710 pKa = 5.78 KK711 pKa = 10.68 EE712 pKa = 4.04 FLTNTVLAGDD722 pKa = 3.84 TTTLRR727 pKa = 11.84 FTISNVHH734 pKa = 5.72 PTADD738 pKa = 3.23 ATITSFTDD746 pKa = 3.15 NLQQNLTGLAATGGPSGNTCGGTLSGTTFLIYY778 pKa = 10.26 TGGSVTSGMSCTIDD792 pKa = 3.52 VEE794 pKa = 4.53 VLVPVGTADD803 pKa = 3.25 GSYY806 pKa = 11.42 GNTTSTLSATQGGGPVVVDD825 pKa = 4.0 PATDD829 pKa = 3.14 ILNVQGLQIEE839 pKa = 4.43 LSKK842 pKa = 11.32 AFTDD846 pKa = 4.42 DD847 pKa = 3.45 PVAPGDD853 pKa = 3.64 NATLEE858 pKa = 4.41 FTLSNLSSTEE868 pKa = 4.12 AISNIAFTDD877 pKa = 3.5 DD878 pKa = 3.76 LAAMLPGTTFASVAGNDD895 pKa = 3.41 CGATVGGTGTSTISVSGTSLAASATCTITVAVTVPGGAVDD935 pKa = 3.52 NTYY938 pKa = 10.96 TNTTSSVTGDD948 pKa = 2.79 IGGLAVTGDD957 pKa = 3.84 PATAQLSVSSILPLAFSKK975 pKa = 10.94 SFSGSVASGEE985 pKa = 4.37 SGTLTFTLSNPNTTALSDD1003 pKa = 4.2 LGFTDD1008 pKa = 5.29 DD1009 pKa = 4.78 LNAMLTGVVATSLPSAPCGLGSSITGTSSLTASGFSLDD1047 pKa = 4.27 ASGGSCTFDD1056 pKa = 2.78 VGFSVPVTATGATYY1070 pKa = 11.21 LNTTSEE1076 pKa = 4.37 LVSFGLSLAPPATANLIVTDD1096 pKa = 4.52 PVADD1100 pKa = 4.37 LSLAIADD1107 pKa = 4.24 SPDD1110 pKa = 3.59 PVTPGQNLVYY1120 pKa = 10.12 TVTASNAGPQTAQSVAVNLPVPTGTTLVSTTGCAEE1155 pKa = 4.41 DD1156 pKa = 3.86 PSGAATCSLGNIASGGNAQYY1176 pKa = 10.53 TLTVTVDD1183 pKa = 3.0 AATSGTVSASGTVSSSTSDD1202 pKa = 3.19 PVAGNNTATASTSAPATADD1221 pKa = 3.12 ISITKK1226 pKa = 10.16 SDD1228 pKa = 3.63 GVTSANSGGTTTYY1241 pKa = 10.78 SIVVANAGPSADD1253 pKa = 3.74 PAVSVADD1260 pKa = 3.92 TFAADD1265 pKa = 4.66 LGCATTSVAAGGATGNTAAFSGDD1288 pKa = 3.07 IAEE1291 pKa = 4.46 TLSMPASSSVTYY1303 pKa = 9.09 TATCDD1308 pKa = 3.17 IAITATGTLSNTATATASVLDD1329 pKa = 4.37 PNATNNSATDD1339 pKa = 3.17 SDD1341 pKa = 4.34 TALVAPVAMGFSKK1354 pKa = 10.76 AFSPATIDD1362 pKa = 3.19 QGANTTLTFTIDD1374 pKa = 3.04 NTANAVAADD1383 pKa = 3.92 NLNFTDD1389 pKa = 5.15 GFPLGVDD1396 pKa = 3.92 LSVAAPVVSSSTCGGTFNPVAGGGNLVFSGGSVAAGATCTVSVLLNARR1444 pKa = 11.84 GSGTATNTSSQLGSFFPTPSAAATADD1470 pKa = 3.23 ITVNPAVAPTFAKK1483 pKa = 10.65 AFGAATIEE1491 pKa = 4.06 QGQTTTATFTINNSANSIEE1510 pKa = 4.14 ASSLQFSDD1518 pKa = 3.71 TLPVEE1523 pKa = 4.44 LVVAATPNASTTCTGGTITATAGSGSILYY1552 pKa = 10.56 SGGEE1556 pKa = 4.03 VAAGATCTVTVTVDD1570 pKa = 3.32 SLGSGSIANPSLNLTSSLPTATAAATPLTINPATAPGVAKK1610 pKa = 9.81 TFTPSAITQGEE1621 pKa = 4.31 TSALSITIDD1630 pKa = 3.12 NSANFLEE1637 pKa = 4.45 MTGVAFTDD1645 pKa = 4.08 AFPSGMVVGPTPGASNTCGGTFSASAGSGTISLSGGTVAAGATCSVGVTVRR1696 pKa = 11.84 GNAAGTLTNPAFDD1709 pKa = 3.67 VTSNIATGTAAATDD1723 pKa = 4.08 LAVNPAAAPGFAKK1736 pKa = 10.65 AFGAGTINQGGTVVATVSVDD1756 pKa = 3.39 NSGNAADD1763 pKa = 4.19 LSGMGFTDD1771 pKa = 4.03 TLPAGLVIAATPAEE1785 pKa = 4.34 TNTCGGSLTANAGASTFALSGGALAGGGTCAVSVTLRR1822 pKa = 11.84 ALEE1825 pKa = 4.51 AGSLTNPMFDD1835 pKa = 3.3 LTSNIATATAASAILTVNAASAPVVAKK1862 pKa = 10.27 GFAPAIINQGQTTTATVSIDD1882 pKa = 3.07 NAANLIEE1889 pKa = 4.25 ATGVAFTDD1897 pKa = 3.85 ALPSGLVVAPTPAGSNTCGGTLTANAGASSFALSGGTIAEE1937 pKa = 4.42 GASCQVTVALRR1948 pKa = 11.84 ALDD1951 pKa = 4.43 GGTLTNPAFDD1961 pKa = 3.88 VTSSIATGTVAATDD1975 pKa = 3.76 LTVNAAAAPGFAKK1988 pKa = 10.46 AFSVSTLDD1996 pKa = 3.5 QGQSLTATVTIDD2008 pKa = 3.32 NAPNAIEE2015 pKa = 4.15 ASNLAFTDD2023 pKa = 3.75 ALPAGLVIGTASGGGSLISTTCGGTASATTGASSFSLSGGTLAEE2067 pKa = 4.56 GASCTVVVVLRR2078 pKa = 11.84 AVGAGTLTNPAMDD2091 pKa = 4.23 LTSSIATATSAPVSLTANAAAAPVVTKK2118 pKa = 9.82 VFAPATIEE2126 pKa = 3.89 QGQTTTATITIDD2138 pKa = 3.15 NAGNSIEE2145 pKa = 4.08 AASLAFTDD2153 pKa = 4.09 TLPAGLQVAAVPNEE2167 pKa = 3.97 SNTCGGTLTAAAGTGAVSLSGGTLAEE2193 pKa = 4.49 GASCAIAVDD2202 pKa = 4.69 LRR2204 pKa = 11.84 ATLPGTLTNPGFDD2217 pKa = 3.59 VTSSIATGSAAATDD2231 pKa = 3.92 LTVNAAAAMGFAKK2244 pKa = 10.4 AFAPATIDD2252 pKa = 3.53 LGSSSTLTFTIDD2264 pKa = 2.95 NSANAIEE2271 pKa = 4.29 AASMAFTDD2279 pKa = 4.28 SFPAGLIVSSAPAATNTCGGTFTATALSGLVSLSGGTLAAGTTCTLSVQVQALTTGSLDD2338 pKa = 3.56 NTSSEE2343 pKa = 4.36 LTSDD2347 pKa = 4.37 LPSASPVATASLTVNAVPITSAASFNPPIIEE2378 pKa = 3.98 LFEE2381 pKa = 4.04 LTEE2384 pKa = 4.78 LVFLFQNDD2392 pKa = 3.17 ADD2394 pKa = 3.98 IDD2396 pKa = 3.94 QILNFIQNLPAGLNVGGAATSRR2418 pKa = 11.84 ANAMAGTNVSTTCSGGTVTAVEE2440 pKa = 5.06 GSTQVQISGATVPANGSCEE2459 pKa = 4.13 LKK2461 pKa = 11.15 VNVIANAIGDD2471 pKa = 4.0 FSNLVGDD2478 pKa = 4.33 ADD2480 pKa = 4.2 LVVNTIARR2488 pKa = 11.84 AFVTYY2493 pKa = 10.6 VVTGSDD2499 pKa = 2.95 GSYY2502 pKa = 10.57 AFSSSATEE2510 pKa = 3.9 LNATVAVAGGTGTQGPLRR2528 pKa = 11.84 SSEE2531 pKa = 3.65 GSYY2534 pKa = 10.93 SVAIAPPTGVAFDD2547 pKa = 4.9 SGVCDD2552 pKa = 5.29 DD2553 pKa = 5.04 GDD2555 pKa = 4.22 SSVDD2559 pKa = 3.63 LANNAIALTLSPLEE2573 pKa = 4.34 SVTCTFTALSPVQQTVDD2590 pKa = 3.88 VINQFLTKK2598 pKa = 10.21 RR2599 pKa = 11.84 ADD2601 pKa = 4.26 LILSTEE2607 pKa = 4.21 PNLGARR2613 pKa = 11.84 GRR2615 pKa = 11.84 DD2616 pKa = 3.05 RR2617 pKa = 11.84 LGRR2620 pKa = 11.84 GFGNASALSFANGDD2634 pKa = 4.04 LKK2636 pKa = 11.71 AMLPFTATMQPGNYY2650 pKa = 9.16 SFKK2653 pKa = 11.03 SSLLQVRR2660 pKa = 11.84 QAAASVQLAHH2670 pKa = 7.21 GSTKK2674 pKa = 10.67 DD2675 pKa = 3.37 AIYY2678 pKa = 10.31 VPNYY2682 pKa = 10.41 RR2683 pKa = 11.84 FDD2685 pKa = 3.39 AWFEE2689 pKa = 3.89 AHH2691 pKa = 6.81 HH2692 pKa = 6.64 KK2693 pKa = 10.77 EE2694 pKa = 4.86 FDD2696 pKa = 3.18 TGTNGTGHH2704 pKa = 6.04 FTAAHH2709 pKa = 6.39 IGADD2713 pKa = 3.43 YY2714 pKa = 11.18 VLNEE2718 pKa = 4.01 NLLIGAMVSFDD2729 pKa = 4.05 SMEE2732 pKa = 5.42 DD2733 pKa = 3.24 DD2734 pKa = 3.83 TAISSASGSGWLIGPYY2750 pKa = 7.9 MTAKK2754 pKa = 10.47 LAPNLYY2760 pKa = 10.08 FDD2762 pKa = 4.57 ARR2764 pKa = 11.84 LAGGASNNKK2773 pKa = 8.6 VSPFNTYY2780 pKa = 9.01 TDD2782 pKa = 3.41 SFDD2785 pKa = 3.5 TRR2787 pKa = 11.84 RR2788 pKa = 11.84 LLASASLSGDD2798 pKa = 3.5 FQQGNWTISPTASLSYY2814 pKa = 10.56 YY2815 pKa = 10.5 RR2816 pKa = 11.84 EE2817 pKa = 4.19 TQDD2820 pKa = 4.61 SYY2822 pKa = 11.69 IDD2824 pKa = 3.55 STGASIPSQTVEE2836 pKa = 4.41 LGQLKK2841 pKa = 10.17 IGPTFTGRR2849 pKa = 11.84 FLGDD2853 pKa = 3.76 DD2854 pKa = 4.05 GEE2856 pKa = 5.58 IIAPYY2861 pKa = 9.88 FSVDD2865 pKa = 3.37 AIYY2868 pKa = 11.21 NLGQTSGVTVTNPDD2882 pKa = 3.47 TASTSGWRR2890 pKa = 11.84 GRR2892 pKa = 11.84 LKK2894 pKa = 10.92 AGVTVTEE2901 pKa = 4.23 EE2902 pKa = 3.87 NGTKK2906 pKa = 10.39 LGIGATYY2913 pKa = 10.74 DD2914 pKa = 3.27 GLFRR2918 pKa = 11.84 SDD2920 pKa = 3.01 FDD2922 pKa = 3.17 AWGLSFEE2929 pKa = 5.52 LEE2931 pKa = 4.07 IPLQKK2936 pKa = 9.32 PTARR2940 pKa = 4.09
Molecular weight: 287.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.668
IPC_protein 3.732
Toseland 3.49
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.389
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.126
Thurlkill 3.541
EMBOSS 3.681
Sillero 3.846
Patrickios 1.062
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A0J9H4A4|A0A0J9H4A4_9RHOB Membrane protein OS=Candidatus Rhodobacter lobularis OX=1675527 GN=AIOL_000659 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4763
0
4763
1428562
37
2940
299.9
32.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.623 ± 0.051
0.942 ± 0.01
5.927 ± 0.031
5.974 ± 0.034
3.832 ± 0.022
8.847 ± 0.039
2.074 ± 0.018
5.071 ± 0.027
3.098 ± 0.029
9.885 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.777 ± 0.017
2.673 ± 0.021
5.053 ± 0.026
3.367 ± 0.021
6.51 ± 0.037
5.076 ± 0.03
5.25 ± 0.029
7.257 ± 0.032
1.481 ± 0.014
2.283 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here