Paramuricea placomus associated circular virus
Average proteome isoelectric point is 9.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RLR9|A0A0K1RLR9_9CIRC Putative capsid protein OS=Paramuricea placomus associated circular virus OX=1692260 PE=4 SV=1
MM1 pKa = 7.31 FGEE4 pKa = 4.16 ADD6 pKa = 3.15 EE7 pKa = 4.89 GEE9 pKa = 4.4 RR10 pKa = 11.84 ANLDD14 pKa = 3.03 IEE16 pKa = 4.39 PPKK19 pKa = 10.28 KK20 pKa = 9.84 RR21 pKa = 11.84 KK22 pKa = 9.76 KK23 pKa = 9.19 RR24 pKa = 11.84 DD25 pKa = 2.96 HH26 pKa = 5.99 RR27 pKa = 11.84 AEE29 pKa = 4.1 GKK31 pKa = 9.96 RR32 pKa = 11.84 RR33 pKa = 11.84 ALRR36 pKa = 11.84 YY37 pKa = 9.39 RR38 pKa = 11.84 NWVFTLNNPQEE49 pKa = 4.39 HH50 pKa = 6.51 GFPRR54 pKa = 11.84 LTSDD58 pKa = 4.62 MIWLRR63 pKa = 11.84 YY64 pKa = 9.03 GEE66 pKa = 4.54 EE67 pKa = 4.09 VAPTTGTPHH76 pKa = 6.02 LQGVVHH82 pKa = 6.68 FKK84 pKa = 10.51 SKK86 pKa = 10.47 RR87 pKa = 11.84 VMPTDD92 pKa = 4.16 LYY94 pKa = 8.67 PWCRR98 pKa = 11.84 NAHH101 pKa = 5.34 WEE103 pKa = 4.2 HH104 pKa = 6.21 MGGTISANLAYY115 pKa = 9.34 TGKK118 pKa = 9.9 EE119 pKa = 3.91 ANEE122 pKa = 4.09 EE123 pKa = 3.95 AGTLHH128 pKa = 6.38 EE129 pKa = 4.86 FGVRR133 pKa = 11.84 PLSNKK138 pKa = 7.19 EE139 pKa = 3.78 ARR141 pKa = 11.84 EE142 pKa = 3.88 KK143 pKa = 10.81 GAAATKK149 pKa = 9.77 EE150 pKa = 3.92 RR151 pKa = 11.84 YY152 pKa = 9.6 INILAHH158 pKa = 6.51 AKK160 pKa = 10.35 AGEE163 pKa = 3.82 FDD165 pKa = 3.78 KK166 pKa = 11.68 LEE168 pKa = 4.06 EE169 pKa = 4.44 EE170 pKa = 4.69 YY171 pKa = 10.54 PGDD174 pKa = 3.91 FLRR177 pKa = 11.84 MYY179 pKa = 8.77 KK180 pKa = 9.15 TLHH183 pKa = 6.83 CIRR186 pKa = 11.84 DD187 pKa = 3.73 GAQGRR192 pKa = 11.84 SEE194 pKa = 4.33 PIARR198 pKa = 11.84 LEE200 pKa = 4.06 HH201 pKa = 5.45 WWIVGPTGCGKK212 pKa = 10.24 SRR214 pKa = 11.84 AVWEE218 pKa = 4.4 TVGASQLYY226 pKa = 10.03 SKK228 pKa = 10.75 LLRR231 pKa = 11.84 TSGGTTTTTKK241 pKa = 9.62 TGSYY245 pKa = 8.55 STTILPYY252 pKa = 10.75 GKK254 pKa = 8.09 TKK256 pKa = 10.12 PVSKK260 pKa = 9.93 IGRR263 pKa = 11.84 TTIPSLRR270 pKa = 11.84 RR271 pKa = 11.84 SRR273 pKa = 11.84 ALQKK277 pKa = 10.76 EE278 pKa = 4.3 SAQLISSSLATTASQTDD295 pKa = 3.4 SSSPMMSSQQ304 pKa = 3.35
Molecular weight: 34.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.12
IPC2_protein 9.019
IPC_protein 8.975
Toseland 9.721
ProMoST 9.443
Dawson 9.955
Bjellqvist 9.648
Wikipedia 10.116
Rodwell 10.306
Grimsley 10.028
Solomon 9.984
Lehninger 9.955
Nozaki 9.765
DTASelect 9.619
Thurlkill 9.809
EMBOSS 10.145
Sillero 9.882
Patrickios 7.629
IPC_peptide 9.984
IPC2_peptide 8.229
IPC2.peptide.svr19 8.057
Protein with the highest isoelectric point:
>tr|A0A0K1RLR9|A0A0K1RLR9_9CIRC Putative capsid protein OS=Paramuricea placomus associated circular virus OX=1692260 PE=4 SV=1
MM1 pKa = 7.63 AYY3 pKa = 9.58 VRR5 pKa = 11.84 KK6 pKa = 9.92 RR7 pKa = 11.84 SFRR10 pKa = 11.84 KK11 pKa = 7.57 RR12 pKa = 11.84 TSWKK16 pKa = 9.94 KK17 pKa = 4.82 MTRR20 pKa = 11.84 GFRR23 pKa = 11.84 KK24 pKa = 9.75 KK25 pKa = 10.4 KK26 pKa = 8.46 PAKK29 pKa = 10.51 ANLNWRR35 pKa = 11.84 KK36 pKa = 9.41 RR37 pKa = 11.84 RR38 pKa = 11.84 SQSFRR43 pKa = 11.84 RR44 pKa = 11.84 ARR46 pKa = 11.84 RR47 pKa = 11.84 TRR49 pKa = 11.84 RR50 pKa = 11.84 AANPGTAIVTSSTFEE65 pKa = 3.85 MNFAGTSPILGILNGTNLAEE85 pKa = 4.21 MGGVASCLVRR95 pKa = 11.84 EE96 pKa = 4.99 LYY98 pKa = 9.99 IQPGRR103 pKa = 11.84 IAGLLARR110 pKa = 11.84 QFVYY114 pKa = 11.06 NMYY117 pKa = 10.23 KK118 pKa = 9.91 IYY120 pKa = 10.48 SVEE123 pKa = 4.02 YY124 pKa = 10.39 KK125 pKa = 10.65 LIRR128 pKa = 11.84 SDD130 pKa = 3.18 KK131 pKa = 10.51 SRR133 pKa = 11.84 ISSGPISADD142 pKa = 3.07 YY143 pKa = 10.63 AYY145 pKa = 10.72 ADD147 pKa = 3.86 FAMLMPNTHH156 pKa = 6.35 NEE158 pKa = 4.04 DD159 pKa = 3.54 FPGISTAGSIQTTRR173 pKa = 11.84 KK174 pKa = 9.53 LMNWMLQQKK183 pKa = 8.92 GSKK186 pKa = 7.93 MVKK189 pKa = 9.38 LQQNFAKK196 pKa = 9.51 MKK198 pKa = 8.91 VPAMVNVEE206 pKa = 5.27 RR207 pKa = 11.84 EE208 pKa = 4.14 FQQNTGTGTVTQMSTIPHH226 pKa = 6.37 PWIEE230 pKa = 3.95 IDD232 pKa = 3.43 TDD234 pKa = 3.48 KK235 pKa = 11.04 MNTMNIGQVIFAMPALNISTFYY257 pKa = 10.66 PVFPTPEE264 pKa = 3.97 AQTAAGTGDD273 pKa = 3.34 VGAPGQTYY281 pKa = 11.19 DD282 pKa = 4.3 NLKK285 pKa = 10.73 KK286 pKa = 10.21 MYY288 pKa = 9.56 DD289 pKa = 3.49 YY290 pKa = 11.26 SIFATVRR297 pKa = 11.84 WGVKK301 pKa = 9.4 GKK303 pKa = 10.94 YY304 pKa = 9.11 IDD306 pKa = 3.72 PAVAQEE312 pKa = 3.84 YY313 pKa = 8.8 HH314 pKa = 6.19 QPSQVGDD321 pKa = 3.98 DD322 pKa = 3.74 EE323 pKa = 4.89 PEE325 pKa = 3.95 EE326 pKa = 4.24 FGEE329 pKa = 4.48 DD330 pKa = 3.6 TVDD333 pKa = 3.19 MGSLIDD339 pKa = 4.06 NMQNMTIAA347 pKa = 3.88
Molecular weight: 39.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.134
IPC2_protein 9.311
IPC_protein 9.311
Toseland 9.94
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.54
Grimsley 10.233
Solomon 10.175
Lehninger 10.145
Nozaki 9.911
DTASelect 9.823
Thurlkill 9.999
EMBOSS 10.35
Sillero 10.072
Patrickios 9.853
IPC_peptide 10.175
IPC2_peptide 8.375
IPC2.peptide.svr19 8.318
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
651
304
347
325.5
36.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.295 ± 0.049
0.614 ± 0.258
3.687 ± 0.504
5.837 ± 1.427
3.379 ± 0.747
7.834 ± 0.498
1.997 ± 0.896
5.069 ± 0.778
6.759 ± 0.103
5.991 ± 1.092
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.147 ± 1.279
4.301 ± 0.93
5.223 ± 0.028
3.994 ± 0.945
7.373 ± 0.59
7.066 ± 0.575
8.449 ± 0.528
4.455 ± 0.808
1.843 ± 0.319
3.687 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here