Hubei virga-like virus 21

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJW8|A0A1L3KJW8_9VIRU Uncharacterized protein OS=Hubei virga-like virus 21 OX=1923336 PE=4 SV=1
MM1 pKa = 7.62SLEE4 pKa = 4.13WCCILALLSCRR15 pKa = 11.84LRR17 pKa = 11.84QLVLVSATPLVHH29 pKa = 7.44RR30 pKa = 11.84RR31 pKa = 11.84IQPLVTFSGVEE42 pKa = 3.74PEE44 pKa = 4.01YY45 pKa = 10.84TLLVDD50 pKa = 3.9HH51 pKa = 6.83LPVVTEE57 pKa = 4.13GEE59 pKa = 4.17KK60 pKa = 11.01CLVAVAGDD68 pKa = 3.82SCEE71 pKa = 4.35LPDD74 pKa = 3.47YY75 pKa = 10.26CGQVEE80 pKa = 4.37RR81 pKa = 11.84FHH83 pKa = 6.89MKK85 pKa = 10.03VQDD88 pKa = 4.21LLLVICAGHH97 pKa = 6.35HH98 pKa = 5.42VLPGHH103 pKa = 6.6KK104 pKa = 9.66SDD106 pKa = 3.86IEE108 pKa = 3.84IFRR111 pKa = 11.84AYY113 pKa = 10.76SLDD116 pKa = 3.18ARR118 pKa = 11.84GEE120 pKa = 4.22FIPVEE125 pKa = 4.17LYY127 pKa = 10.91FIDD130 pKa = 5.02VYY132 pKa = 11.58SLDD135 pKa = 3.12RR136 pKa = 11.84KK137 pKa = 9.54YY138 pKa = 10.61RR139 pKa = 11.84YY140 pKa = 9.89SVVLPWAMLSKK151 pKa = 10.64LVVKK155 pKa = 8.72TAVQHH160 pKa = 5.66SFYY163 pKa = 10.78VDD165 pKa = 2.97KK166 pKa = 10.89DD167 pKa = 3.37LRR169 pKa = 11.84ICRR172 pKa = 11.84RR173 pKa = 11.84GTYY176 pKa = 9.68LRR178 pKa = 11.84SLTRR182 pKa = 11.84LLSRR186 pKa = 11.84RR187 pKa = 11.84ILNSEE192 pKa = 3.77KK193 pKa = 10.23QLYY196 pKa = 9.45PGDD199 pKa = 4.08APFLEE204 pKa = 4.68ANSLRR209 pKa = 11.84GASCRR214 pKa = 11.84PSGTHH219 pKa = 5.89EE220 pKa = 4.75PYY222 pKa = 10.58SCTDD226 pKa = 4.54LIVVNEE232 pKa = 4.47TYY234 pKa = 10.98ALCSSVWTQLHH245 pKa = 6.7PGACPMDD252 pKa = 3.44RR253 pKa = 11.84QYY255 pKa = 11.66RR256 pKa = 11.84RR257 pKa = 11.84GEE259 pKa = 3.84FGFEE263 pKa = 3.89DD264 pKa = 4.09VCTNITVLEE273 pKa = 4.13KK274 pKa = 10.74KK275 pKa = 10.08KK276 pKa = 11.0AFGEE280 pKa = 4.38DD281 pKa = 4.12PDD283 pKa = 5.68DD284 pKa = 3.8GWLQRR289 pKa = 11.84SLKK292 pKa = 10.35QVVNWLTTKK301 pKa = 10.12IDD303 pKa = 3.74DD304 pKa = 3.56FAEE307 pKa = 5.04FIEE310 pKa = 4.7GLFLKK315 pKa = 10.74LLEE318 pKa = 4.15KK319 pKa = 10.82VIAFMFSQLEE329 pKa = 4.21VLDD332 pKa = 4.17SLVEE336 pKa = 4.45FVDD339 pKa = 3.11SRR341 pKa = 11.84YY342 pKa = 9.99VVFEE346 pKa = 4.08LMVVSFIICYY356 pKa = 10.1RR357 pKa = 11.84SNLPAALIFVVIFGVTCGYY376 pKa = 11.06DD377 pKa = 3.0RR378 pKa = 11.84TRR380 pKa = 11.84DD381 pKa = 3.69FRR383 pKa = 11.84LLPEE387 pKa = 4.84LKK389 pKa = 10.52ALLLWSGG396 pKa = 3.46

Molecular weight: 45.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJV3|A0A1L3KJV3_9VIRU Uncharacterized protein OS=Hubei virga-like virus 21 OX=1923336 PE=4 SV=1
MM1 pKa = 7.22VNGAFRR7 pKa = 11.84RR8 pKa = 11.84SRR10 pKa = 11.84ARR12 pKa = 11.84ATVARR17 pKa = 11.84RR18 pKa = 11.84SFLDD22 pKa = 3.1SLLVVLLRR30 pKa = 11.84VVAHH34 pKa = 6.11PVSLVLAVFLVLFVAAEE51 pKa = 4.18VLEE54 pKa = 4.46TTGPLEE60 pKa = 4.97SLDD63 pKa = 3.96KK64 pKa = 11.03LIKK67 pKa = 10.19QEE69 pKa = 5.06LGSKK73 pKa = 10.14DD74 pKa = 3.33INSLEE79 pKa = 3.95KK80 pKa = 10.5FLLKK84 pKa = 10.91GFDD87 pKa = 3.16KK88 pKa = 11.24CIVFVILYY96 pKa = 5.85KK97 pKa = 9.98TKK99 pKa = 10.5VVATLAYY106 pKa = 10.31SIFVALNPTKK116 pKa = 10.59LRR118 pKa = 11.84WSVFGAAVLIVVAIPSLPVFYY139 pKa = 10.59HH140 pKa = 7.08IITAVALVFYY150 pKa = 10.53LALQRR155 pKa = 11.84IEE157 pKa = 4.99HH158 pKa = 6.57KK159 pKa = 10.63ALTVFIYY166 pKa = 9.97VAGMVLYY173 pKa = 9.03TSAVVVSVATAGAGKK188 pKa = 10.14RR189 pKa = 11.84NSTGAPP195 pKa = 3.29

Molecular weight: 21.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3205

170

2444

801.3

90.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.27 ± 1.251

2.059 ± 0.506

6.115 ± 0.857

5.367 ± 0.49

4.618 ± 0.437

5.491 ± 0.668

2.559 ± 0.349

5.523 ± 0.27

5.086 ± 0.317

9.735 ± 1.731

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.309 ± 0.463

2.964 ± 0.579

4.399 ± 0.272

3.401 ± 0.622

6.739 ± 0.651

6.646 ± 0.053

5.71 ± 0.611

8.924 ± 1.751

0.78 ± 0.23

4.306 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski