Honeysuckle yellow vein betasatellite
Average proteome isoelectric point is 4.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7T470|Q7T470_9VIRU C1 protein OS=Honeysuckle yellow vein betasatellite OX=221037 GN=C1 PE=4 SV=1
MM1 pKa = 7.45 TIIYY5 pKa = 10.74 NNGNGIKK12 pKa = 10.29 FIIDD16 pKa = 3.05 VRR18 pKa = 11.84 LQQFIRR24 pKa = 11.84 VQVQVYY30 pKa = 7.48 STNQPVLTGIRR41 pKa = 11.84 CNIPYY46 pKa = 9.25 TYY48 pKa = 10.99 VQMVPPFDD56 pKa = 4.36 FNGTEE61 pKa = 3.9 EE62 pKa = 4.28 LVRR65 pKa = 11.84 DD66 pKa = 4.27 TLEE69 pKa = 4.62 LMYY72 pKa = 10.9 DD73 pKa = 3.99 DD74 pKa = 5.88 SDD76 pKa = 3.33 ISNFKK81 pKa = 9.67 QEE83 pKa = 4.06 EE84 pKa = 4.41 MIDD87 pKa = 4.07 SIDD90 pKa = 3.54 MVMMHH95 pKa = 7.47 WLSDD99 pKa = 3.56 MGVDD103 pKa = 3.34 TVDD106 pKa = 3.32 RR107 pKa = 11.84 YY108 pKa = 9.43 VVRR111 pKa = 11.84 NTYY114 pKa = 7.55 TVV116 pKa = 2.82
Molecular weight: 13.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.102
IPC2_protein 4.228
IPC_protein 4.151
Toseland 3.948
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.088
Rodwell 3.986
Grimsley 3.859
Solomon 4.139
Lehninger 4.101
Nozaki 4.266
DTASelect 4.507
Thurlkill 3.999
EMBOSS 4.101
Sillero 4.279
Patrickios 2.994
IPC_peptide 4.139
IPC2_peptide 4.253
IPC2.peptide.svr19 4.193
Protein with the highest isoelectric point:
>tr|Q7T470|Q7T470_9VIRU C1 protein OS=Honeysuckle yellow vein betasatellite OX=221037 GN=C1 PE=4 SV=1
MM1 pKa = 7.45 TIIYY5 pKa = 10.74 NNGNGIKK12 pKa = 10.29 FIIDD16 pKa = 3.05 VRR18 pKa = 11.84 LQQFIRR24 pKa = 11.84 VQVQVYY30 pKa = 7.48 STNQPVLTGIRR41 pKa = 11.84 CNIPYY46 pKa = 9.25 TYY48 pKa = 10.99 VQMVPPFDD56 pKa = 4.36 FNGTEE61 pKa = 3.9 EE62 pKa = 4.28 LVRR65 pKa = 11.84 DD66 pKa = 4.27 TLEE69 pKa = 4.62 LMYY72 pKa = 10.9 DD73 pKa = 3.99 DD74 pKa = 5.88 SDD76 pKa = 3.33 ISNFKK81 pKa = 9.67 QEE83 pKa = 4.06 EE84 pKa = 4.41 MIDD87 pKa = 4.07 SIDD90 pKa = 3.54 MVMMHH95 pKa = 7.47 WLSDD99 pKa = 3.56 MGVDD103 pKa = 3.34 TVDD106 pKa = 3.32 RR107 pKa = 11.84 YY108 pKa = 9.43 VVRR111 pKa = 11.84 NTYY114 pKa = 7.55 TVV116 pKa = 2.82
Molecular weight: 13.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.102
IPC2_protein 4.228
IPC_protein 4.151
Toseland 3.948
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.088
Rodwell 3.986
Grimsley 3.859
Solomon 4.139
Lehninger 4.101
Nozaki 4.266
DTASelect 4.507
Thurlkill 3.999
EMBOSS 4.101
Sillero 4.279
Patrickios 2.994
IPC_peptide 4.139
IPC2_peptide 4.253
IPC2.peptide.svr19 4.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
116
116
116
116.0
13.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
0.0 ± 0.0
0.862 ± 0.0
9.483 ± 0.0
4.31 ± 0.0
4.31 ± 0.0
4.31 ± 0.0
0.862 ± 0.0
9.483 ± 0.0
1.724 ± 0.0
5.172 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
6.897 ± 0.0
6.897 ± 0.0
3.448 ± 0.0
6.034 ± 0.0
5.172 ± 0.0
4.31 ± 0.0
7.759 ± 0.0
12.069 ± 0.0
0.862 ± 0.0
6.034 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here