Meiothermus granaticius NBRC 107808
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3220 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A399F108|A0A399F108_9DEIN Uncharacterized protein OS=Meiothermus granaticius NBRC 107808 OX=1227551 GN=Mgrana_03245 PE=4 SV=1
MM1 pKa = 7.15 KK2 pKa = 10.14 QVALLSSILALAFAGCGLISSPPIDD27 pKa = 4.79 NPFQLAGTSTTLNLVSNQATGTATVTATFDD57 pKa = 4.36 DD58 pKa = 4.36 LTNLNLPATPSGFNYY73 pKa = 10.26 NLAITGVSFGSGCPATIPSPINVTMSATLTVSDD106 pKa = 4.45 NGGSGNTQRR115 pKa = 11.84 SSTASASNVQFTLTNNGGQIAVGNLTNGTFNFNAGQLLSIIQTGGPNTANLQAQVTTVSTPDD177 pKa = 3.34 LAGCAMTLTWGGGQGILKK195 pKa = 9.78 FF196 pKa = 3.95
Molecular weight: 19.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.933
IPC2_protein 4.342
IPC_protein 4.075
Toseland 3.846
ProMoST 4.317
Dawson 4.113
Bjellqvist 4.279
Wikipedia 4.164
Rodwell 3.91
Grimsley 3.77
Solomon 4.088
Lehninger 4.037
Nozaki 4.266
DTASelect 4.584
Thurlkill 3.973
EMBOSS 4.151
Sillero 4.202
Patrickios 2.015
IPC_peptide 4.075
IPC2_peptide 4.164
IPC2.peptide.svr19 4.11
Protein with the highest isoelectric point:
>tr|A0A399FC72|A0A399FC72_9DEIN Lipopolysaccharide export system ATP-binding protein LptB OS=Meiothermus granaticius NBRC 107808 OX=1227551 GN=lptB_2 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.47 RR3 pKa = 11.84 TWQPNKK9 pKa = 9.68 RR10 pKa = 11.84 KK11 pKa = 9.52 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.29 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.43 TANGRR28 pKa = 11.84 KK29 pKa = 8.52 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 VKK41 pKa = 10.03 LTVSSEE47 pKa = 4.12 PQLKK51 pKa = 9.94 RR52 pKa = 3.34
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.444
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.179
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.076
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3220
0
3220
987299
29
2789
306.6
33.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.006 ± 0.046
0.525 ± 0.011
3.931 ± 0.03
6.615 ± 0.053
3.576 ± 0.029
8.922 ± 0.04
1.945 ± 0.022
4.048 ± 0.033
3.287 ± 0.037
12.843 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.853 ± 0.02
2.52 ± 0.025
5.883 ± 0.038
4.193 ± 0.03
6.963 ± 0.041
5.166 ± 0.031
4.798 ± 0.037
7.445 ± 0.034
1.612 ± 0.021
2.87 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here