Streptomyces phage Sebastisaurus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411B3V1|A0A411B3V1_9CAUD Uncharacterized protein OS=Streptomyces phage Sebastisaurus OX=2510572 GN=40 PE=4 SV=1
MM1 pKa = 7.3NNKK4 pKa = 9.04FVLPVSSLASAAVAFGLGAMIFTNGNTPADD34 pKa = 3.67AAKK37 pKa = 10.12PGPTPTVTKK46 pKa = 10.38SVPANEE52 pKa = 4.39KK53 pKa = 10.53AADD56 pKa = 3.57AHH58 pKa = 6.3EE59 pKa = 4.72KK60 pKa = 9.39STTDD64 pKa = 3.26AEE66 pKa = 4.37AVPASAGAKK75 pKa = 10.34GPFKK79 pKa = 10.61DD80 pKa = 3.54AQGDD84 pKa = 3.97GKK86 pKa = 11.25YY87 pKa = 10.65LDD89 pKa = 5.07DD90 pKa = 4.36FGKK93 pKa = 10.68AVMPNGVGVHH103 pKa = 5.61VPDD106 pKa = 5.88ALLPWQEE113 pKa = 4.15QDD115 pKa = 4.37HH116 pKa = 6.73SDD118 pKa = 3.36HH119 pKa = 6.08TTDD122 pKa = 5.02AGEE125 pKa = 4.31LPPGVVPAQPEE136 pKa = 4.33GTYY139 pKa = 10.09TDD141 pKa = 4.37PDD143 pKa = 3.89AGTDD147 pKa = 3.36TSDD150 pKa = 3.94DD151 pKa = 3.98VVSGSPGEE159 pKa = 4.27STGTGSTGTNSGSTYY174 pKa = 10.83EE175 pKa = 4.21EE176 pKa = 4.35AQPAPAPAVQAPSGVVRR193 pKa = 11.84SGGLPSGVVPVGPVLNSVTGVVSSAVTEE221 pKa = 4.17

Molecular weight: 21.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411B3V5|A0A411B3V5_9CAUD Uncharacterized protein OS=Streptomyces phage Sebastisaurus OX=2510572 GN=41 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84TRR4 pKa = 11.84CLEE7 pKa = 3.86CRR9 pKa = 11.84EE10 pKa = 4.15WATHH14 pKa = 5.67SGRR17 pKa = 11.84CALHH21 pKa = 6.21HH22 pKa = 5.37SHH24 pKa = 5.32YY25 pKa = 8.57TARR28 pKa = 11.84RR29 pKa = 11.84SVQSHH34 pKa = 5.63AKK36 pKa = 9.38RR37 pKa = 11.84RR38 pKa = 11.84EE39 pKa = 3.95AIARR43 pKa = 11.84GNNAAARR50 pKa = 11.84LRR52 pKa = 11.84KK53 pKa = 9.31AVRR56 pKa = 11.84KK57 pKa = 9.99AMAGQCVMCLRR68 pKa = 11.84VFLPSQVDD76 pKa = 3.18IDD78 pKa = 4.19HH79 pKa = 7.19KK80 pKa = 11.24LPLARR85 pKa = 11.84GGEE88 pKa = 4.5DD89 pKa = 2.77IDD91 pKa = 5.75SNVQVLCKK99 pKa = 10.11SCHH102 pKa = 4.35KK103 pKa = 9.44TKK105 pKa = 10.05TAMDD109 pKa = 4.17FGKK112 pKa = 10.68RR113 pKa = 11.84PFF115 pKa = 3.78

Molecular weight: 13.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12128

58

830

233.2

25.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.906 ± 0.453

0.8 ± 0.135

6.514 ± 0.222

6.077 ± 0.423

3.133 ± 0.183

8.353 ± 0.406

1.657 ± 0.171

3.463 ± 0.295

4.634 ± 0.368

7.982 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.103 ± 0.108

3.092 ± 0.149

5.483 ± 0.342

2.672 ± 0.169

6.621 ± 0.446

5.698 ± 0.24

7.297 ± 0.41

8.163 ± 0.229

1.723 ± 0.141

2.63 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski