Polystyrenella longa
Average proteome isoelectric point is 5.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4607 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
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Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A518CTS8|A0A518CTS8_9PLAN Uncharacterized protein OS=Polystyrenella longa OX=2528007 GN=Pla110_43690 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.09 PFFWFEE8 pKa = 3.79 SLRR11 pKa = 11.84 NKK13 pKa = 9.74 LRR15 pKa = 11.84 NNTEE19 pKa = 3.3 KK20 pKa = 10.8 RR21 pKa = 11.84 KK22 pKa = 8.78 VLKK25 pKa = 10.19 RR26 pKa = 11.84 HH27 pKa = 5.73 RR28 pKa = 11.84 VDD30 pKa = 3.35 HH31 pKa = 5.8 KK32 pKa = 10.81 QIPASVEE39 pKa = 4.01 SLEE42 pKa = 3.97 EE43 pKa = 3.9 RR44 pKa = 11.84 LLLSVTTTDD53 pKa = 3.98 IDD55 pKa = 4.1 PNPADD60 pKa = 3.67 TSTSATSNVSPEE72 pKa = 4.42 SIDD75 pKa = 4.06 LQLLLNQSITEE86 pKa = 4.4 TVTLTLPTGGIGEE99 pKa = 5.05 EE100 pKa = 4.34 DD101 pKa = 3.91 PQVPTADD108 pKa = 3.48 VIFVVDD114 pKa = 5.16 EE115 pKa = 4.15 SASMGGEE122 pKa = 3.91 HH123 pKa = 6.24 SWISEE128 pKa = 3.96 MVLDD132 pKa = 5.69 LEE134 pKa = 5.17 TALEE138 pKa = 4.2 DD139 pKa = 3.89 QGIFNNRR146 pKa = 11.84 FALVGYY152 pKa = 9.39 LDD154 pKa = 4.1 KK155 pKa = 11.6 GSVFNMAPSVDD166 pKa = 3.68 QISIYY171 pKa = 10.42 DD172 pKa = 3.58 EE173 pKa = 4.16 SGAIVDD179 pKa = 3.5 KK180 pKa = 10.49 GTVKK184 pKa = 10.47 RR185 pKa = 11.84 EE186 pKa = 3.68 YY187 pKa = 10.61 RR188 pKa = 11.84 DD189 pKa = 3.9 DD190 pKa = 3.97 GSGNVGYY197 pKa = 10.72 VRR199 pKa = 11.84 AEE201 pKa = 4.0 FNLPADD207 pKa = 3.66 GTYY210 pKa = 10.71 YY211 pKa = 11.13 AVLNTQQLDD220 pKa = 3.64 LLGEE224 pKa = 4.09 VSIEE228 pKa = 3.77 AEE230 pKa = 4.13 NYY232 pKa = 7.46 KK233 pKa = 9.17 TATTGSLGDD242 pKa = 3.32 TWTVEE247 pKa = 4.18 DD248 pKa = 4.81 NYY250 pKa = 10.66 VASSGQQIASGPDD263 pKa = 2.72 NGTSYY268 pKa = 11.76 GSGSVNNAPRR278 pKa = 11.84 LDD280 pKa = 3.57 YY281 pKa = 10.97 DD282 pKa = 3.76 INFDD286 pKa = 3.88 EE287 pKa = 4.96 ADD289 pKa = 3.69 SYY291 pKa = 9.65 YY292 pKa = 10.68 VWIRR296 pKa = 11.84 GEE298 pKa = 4.08 RR299 pKa = 11.84 PDD301 pKa = 3.69 TNGDD305 pKa = 3.62 QIHH308 pKa = 6.63 FGLNGSRR315 pKa = 11.84 ISSINSFNFWEE326 pKa = 4.53 NNLGWAEE333 pKa = 4.16 AGTRR337 pKa = 11.84 ITVPSTGIHH346 pKa = 5.83 TLNLWMGEE354 pKa = 3.94 DD355 pKa = 4.96 GINIDD360 pKa = 5.35 KK361 pKa = 10.7 IVLTKK366 pKa = 10.76 DD367 pKa = 3.14 PNFNPTGQGPSEE379 pKa = 4.25 SVQDD383 pKa = 3.65 QNGVFLQNTSSHH395 pKa = 6.5 EE396 pKa = 3.82 YY397 pKa = 10.95 GFYY400 pKa = 11.08 LEE402 pKa = 5.56 DD403 pKa = 3.41 NSAAITNAALSFDD416 pKa = 3.59 TAYY419 pKa = 10.88 QGVTTKK425 pKa = 10.96 AGIDD429 pKa = 3.3 HH430 pKa = 7.3 TYY432 pKa = 11.22 SFTTGSTTDD441 pKa = 4.04 LLFEE445 pKa = 4.78 PLSNDD450 pKa = 3.32 EE451 pKa = 4.14 NYY453 pKa = 9.74 EE454 pKa = 3.87 WSLSGPSGEE463 pKa = 4.04 IVADD467 pKa = 3.54 KK468 pKa = 11.03 GFNLADD474 pKa = 3.73 TLLSATASGTYY485 pKa = 9.49 TLTIRR490 pKa = 11.84 ATGPTNGPYY499 pKa = 10.75 GFRR502 pKa = 11.84 LVDD505 pKa = 3.77 LTTPASLTTGTTTTATLDD523 pKa = 3.44 PVAEE527 pKa = 4.2 SKK529 pKa = 10.87 VYY531 pKa = 10.56 SLAMVAGSQYY541 pKa = 10.89 YY542 pKa = 10.42 FNEE545 pKa = 4.39 TGWDD549 pKa = 3.43 GTANARR555 pKa = 11.84 WRR557 pKa = 11.84 LVDD560 pKa = 4.38 DD561 pKa = 4.31 NGAVLFDD568 pKa = 4.22 SSLSDD573 pKa = 3.53 DD574 pKa = 4.46 HH575 pKa = 8.72 DD576 pKa = 5.9 LITATSTATHH586 pKa = 5.52 YY587 pKa = 11.01 LIVEE591 pKa = 4.36 GDD593 pKa = 3.19 IHH595 pKa = 5.86 EE596 pKa = 4.62 TASTARR602 pKa = 11.84 SFTFTVEE609 pKa = 4.17 DD610 pKa = 3.93 LGSVSSATQALTLGTAINSSLTQIGEE636 pKa = 4.01 QAHH639 pKa = 4.84 YY640 pKa = 9.49 TFTLASDD647 pKa = 3.48 ALLYY651 pKa = 10.5 FDD653 pKa = 5.45 SLTNSTYY660 pKa = 11.2 LNWSLTGPSGEE671 pKa = 3.82 IVVNQDD677 pKa = 2.98 FTGGNQVLNLSSGNYY692 pKa = 8.21 TLTVDD697 pKa = 3.76 GTEE700 pKa = 3.86 TAIGSFHH707 pKa = 7.31 FNLQSLSTLATTMTPGIAVTDD728 pKa = 3.74 TLDD731 pKa = 3.94 PEE733 pKa = 4.78 TEE735 pKa = 3.9 SHH737 pKa = 6.64 VYY739 pKa = 9.7 QFSAATGGMYY749 pKa = 10.52 SFDD752 pKa = 3.81 SQSWTGSANASWKK765 pKa = 10.76 LVDD768 pKa = 4.16 PYY770 pKa = 11.8 GNILFDD776 pKa = 3.68 EE777 pKa = 4.55 TLDD780 pKa = 3.8 TDD782 pKa = 3.57 VSTLHH787 pKa = 5.49 FHH789 pKa = 7.26 EE790 pKa = 5.64 GGTYY794 pKa = 10.01 SLIVEE799 pKa = 4.29 GDD801 pKa = 3.42 EE802 pKa = 5.29 AEE804 pKa = 4.31 TSTTPGYY811 pKa = 9.02 TINIVAHH818 pKa = 5.83 GTDD821 pKa = 3.33 TSTPAPLDD829 pKa = 3.45 LSQVVSGDD837 pKa = 2.85 ISASNEE843 pKa = 3.31 QDD845 pKa = 2.86 TFTFTVASAGLFAFDD860 pKa = 4.92 ALTDD864 pKa = 3.72 NGPVRR869 pKa = 11.84 WSLTGSTSGTVVSNRR884 pKa = 11.84 LFSASNGKK892 pKa = 8.99 NVSDD896 pKa = 5.1 ANATLNLAAEE906 pKa = 5.04 TYY908 pKa = 9.93 TLTIDD913 pKa = 4.17 ADD915 pKa = 3.88 TDD917 pKa = 3.75 VTGDD921 pKa = 3.52 YY922 pKa = 11.29 AFALIDD928 pKa = 4.17 LGASATTLTAGTALNPRR945 pKa = 11.84 FNSHH949 pKa = 7.26 AIKK952 pKa = 10.02 PYY954 pKa = 10.74 KK955 pKa = 9.37 FTATANDD962 pKa = 4.08 RR963 pKa = 11.84 LHH965 pKa = 6.99 LLLNNWTGTTEE976 pKa = 4.24 SQWRR980 pKa = 11.84 VVDD983 pKa = 3.86 DD984 pKa = 4.41 AGTVVASADD993 pKa = 3.75 LNQGTGAITLTAGGTYY1009 pKa = 10.54 YY1010 pKa = 11.46 LLLEE1014 pKa = 4.74 GDD1016 pKa = 3.5 QSEE1019 pKa = 4.68 TVLNSSVNVEE1029 pKa = 3.94 IEE1031 pKa = 4.18 LEE1033 pKa = 4.19 EE1034 pKa = 4.32 VTTSATLVMSTPTTGNIASVGDD1056 pKa = 3.27 KK1057 pKa = 10.86 HH1058 pKa = 6.92 EE1059 pKa = 4.18 YY1060 pKa = 9.96 TFTGTEE1066 pKa = 4.01 GDD1068 pKa = 3.77 TVLVDD1073 pKa = 4.04 FTSAATTDD1081 pKa = 3.3 HH1082 pKa = 6.73 QITLRR1087 pKa = 11.84 GPNGEE1092 pKa = 4.21 TLFVSNSHH1100 pKa = 6.76 PANVGIQVTDD1110 pKa = 4.07 LEE1112 pKa = 4.37 LSEE1115 pKa = 4.31 TGTYY1119 pKa = 10.25 QLVISAEE1126 pKa = 4.15 DD1127 pKa = 3.54 SSTGAYY1133 pKa = 8.39 EE1134 pKa = 4.05 FQVSVRR1140 pKa = 11.84 DD1141 pKa = 4.01 DD1142 pKa = 3.56 LQMDD1146 pKa = 4.01 HH1147 pKa = 6.23 EE1148 pKa = 4.9 VKK1150 pKa = 10.5 GVYY1153 pKa = 10.05 DD1154 pKa = 3.19 SFYY1157 pKa = 9.2 TRR1159 pKa = 11.84 EE1160 pKa = 3.89 VFKK1163 pKa = 11.22 FNGLEE1168 pKa = 4.08 GQSIQLKK1175 pKa = 8.11 MDD1177 pKa = 3.24 AWGTAADD1184 pKa = 4.26 FGYY1187 pKa = 10.59 AAKK1190 pKa = 10.46 RR1191 pKa = 11.84 LSTLGGDD1198 pKa = 3.39 EE1199 pKa = 4.76 DD1200 pKa = 5.02 GYY1202 pKa = 11.71 NAIEE1206 pKa = 4.73 LGLNLLDD1213 pKa = 4.23 FRR1215 pKa = 11.84 EE1216 pKa = 4.58 DD1217 pKa = 3.4 ASKK1220 pKa = 10.7 HH1221 pKa = 5.65 IILITDD1227 pKa = 3.52 EE1228 pKa = 5.3 DD1229 pKa = 4.51 RR1230 pKa = 11.84 DD1231 pKa = 3.92 NFDD1234 pKa = 4.0 NNLTASTLQADD1245 pKa = 5.14 LEE1247 pKa = 4.28 AQGINLHH1254 pKa = 6.76 SILNMQIDD1262 pKa = 3.81 STDD1265 pKa = 3.51 QVDD1268 pKa = 3.92 LALGVNGDD1276 pKa = 4.13 SPDD1279 pKa = 3.32 SRR1281 pKa = 11.84 AYY1283 pKa = 10.61 FPDD1286 pKa = 3.98 GNGGYY1291 pKa = 9.62 ISEE1294 pKa = 4.44 PSGGVTFNRR1303 pKa = 11.84 YY1304 pKa = 9.21 SSPSYY1309 pKa = 10.52 EE1310 pKa = 3.95 YY1311 pKa = 10.15 TSNVDD1316 pKa = 3.4 TQVDD1320 pKa = 4.39 YY1321 pKa = 11.23 INPTLAVEE1329 pKa = 4.26 GTIWDD1334 pKa = 3.82 LRR1336 pKa = 11.84 QLRR1339 pKa = 11.84 SGGTLGTTFATALSFQEE1356 pKa = 4.48 AFVDD1360 pKa = 4.78 VITEE1364 pKa = 4.1 EE1365 pKa = 4.43 VADD1368 pKa = 4.36 HH1369 pKa = 6.45 YY1370 pKa = 11.76 YY1371 pKa = 11.22 SLALSKK1377 pKa = 10.97 SGVDD1381 pKa = 4.0 FAFSAATFTEE1391 pKa = 4.45 EE1392 pKa = 4.06 TVSFDD1397 pKa = 3.51 VTFTEE1402 pKa = 4.74 NGASHH1407 pKa = 7.28 IFDD1410 pKa = 3.8 LQFIQSGDD1418 pKa = 3.25 TANPFGSLPVSLTSNYY1434 pKa = 9.67 VKK1436 pKa = 10.49 LAEE1439 pKa = 4.12 EE1440 pKa = 4.41 ADD1442 pKa = 3.96 FNTAYY1447 pKa = 11.01 SDD1449 pKa = 3.58 TFTLDD1454 pKa = 2.69 SGTNALALRR1463 pKa = 11.84 FNNLNFDD1470 pKa = 3.63 TSTVDD1475 pKa = 3.72 EE1476 pKa = 4.82 INDD1479 pKa = 3.63 AFEE1482 pKa = 4.35 LALVDD1487 pKa = 4.27 SNGDD1491 pKa = 3.64 PVVGTILQNRR1501 pKa = 11.84 DD1502 pKa = 2.41 AFFNYY1507 pKa = 9.89 TEE1509 pKa = 4.85 GEE1511 pKa = 4.24 TPNYY1515 pKa = 9.76 GNGVLFEE1522 pKa = 4.34 TDD1524 pKa = 2.66 ISNSFSTVTVDD1535 pKa = 4.45 VSHH1538 pKa = 6.51 LTAGTSLTLIARR1550 pKa = 11.84 LVNDD1554 pKa = 5.15 DD1555 pKa = 4.08 NDD1557 pKa = 3.8 STTSVIIDD1565 pKa = 3.63 PRR1567 pKa = 11.84 LLQLDD1572 pKa = 3.83 VSPVVNPTTTSPTEE1586 pKa = 4.03 ANNTPQTIYY1595 pKa = 9.25 PARR1598 pKa = 11.84 MEE1600 pKa = 4.78 DD1601 pKa = 3.65 VTDD1604 pKa = 4.07 LTTSSYY1610 pKa = 10.96 RR1611 pKa = 11.84 ATSFNEE1617 pKa = 4.06 EE1618 pKa = 3.76 TDD1620 pKa = 3.61 VLYY1623 pKa = 11.0 SAVEE1627 pKa = 3.9 IEE1629 pKa = 4.26 NTSAEE1634 pKa = 4.27 ILRR1637 pKa = 11.84 GPILIGVQNISNVNVSLTNIDD1658 pKa = 3.18 GTLPDD1663 pKa = 3.74 GTPYY1667 pKa = 11.18 YY1668 pKa = 10.42 DD1669 pKa = 3.68 ISHH1672 pKa = 7.56 LLTGDD1677 pKa = 2.78 VWDD1680 pKa = 3.94 PTEE1683 pKa = 3.99 VLSGFSLQFTNSNQVQFDD1701 pKa = 3.72 FDD1703 pKa = 4.23 LVVYY1707 pKa = 10.11 ADD1709 pKa = 3.8 YY1710 pKa = 11.02 MEE1712 pKa = 5.56 TITGAPTVNEE1722 pKa = 4.01 GSTYY1726 pKa = 9.72 TLNLAVSDD1734 pKa = 4.33 FNVSSWVIDD1743 pKa = 3.31 WGDD1746 pKa = 3.79 GGSSTITGGTLTATHH1761 pKa = 7.31 TYY1763 pKa = 11.29 ADD1765 pKa = 3.93 GPNNYY1770 pKa = 8.72 TITSTASDD1778 pKa = 3.43 GTLIVDD1784 pKa = 3.89 TNDD1787 pKa = 3.16 LSVAVANVDD1796 pKa = 3.51 PVLTISGAQSVVQDD1810 pKa = 3.3 STYY1813 pKa = 10.93 PLGLSVTDD1821 pKa = 4.81 PGDD1824 pKa = 3.6 DD1825 pKa = 3.65 TLTEE1829 pKa = 4.19 WVIDD1833 pKa = 3.71 WGDD1836 pKa = 3.47 GTSPQTIPGTSLAVDD1851 pKa = 3.66 HH1852 pKa = 6.69 VFTSPGAYY1860 pKa = 9.02 TITATASDD1868 pKa = 3.73 EE1869 pKa = 4.49 DD1870 pKa = 3.93 GTYY1873 pKa = 10.8 SSNSLIVQVGEE1884 pKa = 3.94 VFLIEE1889 pKa = 4.01 NSHH1892 pKa = 6.14 FVVEE1896 pKa = 4.36 HH1897 pKa = 6.72 RR1898 pKa = 11.84 YY1899 pKa = 10.01 EE1900 pKa = 4.09 FTLDD1904 pKa = 3.0 EE1905 pKa = 4.05 STAAVEE1911 pKa = 4.22 VQTYY1915 pKa = 11.35 DD1916 pKa = 4.8 LFFDD1920 pKa = 3.93 TNDD1923 pKa = 4.34 LISINDD1929 pKa = 3.7 AFEE1932 pKa = 4.22 LALVDD1937 pKa = 4.05 SEE1939 pKa = 4.3 GDD1941 pKa = 3.87 SVVGTIIAGRR1951 pKa = 11.84 DD1952 pKa = 2.95 AFYY1955 pKa = 11.01 NQTEE1959 pKa = 4.33 GEE1961 pKa = 4.05 LRR1963 pKa = 11.84 RR1964 pKa = 11.84 LGGGAHH1970 pKa = 6.02 YY1971 pKa = 10.56 NAGTGAVTIDD1981 pKa = 3.53 VSHH1984 pKa = 6.77 LAVGTEE1990 pKa = 3.82 LTLIARR1996 pKa = 11.84 LVNNDD2001 pKa = 4.08 DD2002 pKa = 3.78 DD2003 pKa = 4.37 TTTSVGLGEE2012 pKa = 5.49 DD2013 pKa = 3.63 LTFLTVSPVVGPTTTTPIPVEE2034 pKa = 4.11 TLSQAINLNEE2044 pKa = 4.47 LDD2046 pKa = 4.93 DD2047 pKa = 4.02 VTNSIGVAYY2056 pKa = 8.9 TVTSFNEE2063 pKa = 3.81 EE2064 pKa = 3.81 TDD2066 pKa = 3.34 RR2067 pKa = 11.84 LTTGIQLTNEE2077 pKa = 3.76 GTYY2080 pKa = 9.74 SVRR2083 pKa = 11.84 GPLLMGINGITAAHH2097 pKa = 5.65 VRR2099 pKa = 11.84 LEE2101 pKa = 4.12 NADD2104 pKa = 3.47 VVGPDD2109 pKa = 3.04 GTRR2112 pKa = 11.84 YY2113 pKa = 10.2 YY2114 pKa = 11.08 DD2115 pKa = 3.67 LSQYY2119 pKa = 11.17 LFDD2122 pKa = 4.96 EE2123 pKa = 4.2 QDD2125 pKa = 2.89 SWFEE2129 pKa = 3.8 PSEE2132 pKa = 4.32 TTTEE2136 pKa = 4.24 EE2137 pKa = 3.63 IFLTFFNPNHH2147 pKa = 5.98 NQFDD2151 pKa = 3.94 YY2152 pKa = 11.41 DD2153 pKa = 3.64 IVVLAKK2159 pKa = 10.68 LNSAPLFTTSPVVEE2173 pKa = 4.87 VIAGEE2178 pKa = 4.31 DD2179 pKa = 3.45 YY2180 pKa = 11.1 SYY2182 pKa = 11.71 DD2183 pKa = 3.49 SDD2185 pKa = 4.71 AVDD2188 pKa = 3.66 PEE2190 pKa = 4.32 EE2191 pKa = 6.44 DD2192 pKa = 3.44 EE2193 pKa = 4.34 VTYY2196 pKa = 11.2 SLVSGPTGLTIDD2208 pKa = 3.83 EE2209 pKa = 4.51 TTGIVSWTTSLTDD2222 pKa = 3.48 ANSIEE2227 pKa = 4.4 SVVIQAQDD2235 pKa = 3.57 PYY2237 pKa = 11.58 AGTTTQEE2244 pKa = 3.81 FSIHH2248 pKa = 5.83 VRR2250 pKa = 11.84 DD2251 pKa = 4.17 TVANRR2256 pKa = 11.84 PPRR2259 pKa = 11.84 FTSIPVLDD2267 pKa = 4.53 AYY2269 pKa = 11.17 VGLEE2273 pKa = 3.78 YY2274 pKa = 9.93 TYY2276 pKa = 10.94 QATADD2281 pKa = 4.3 DD2282 pKa = 4.86 PDD2284 pKa = 5.54 GDD2286 pKa = 4.14 SLVFAGYY2293 pKa = 8.49 EE2294 pKa = 3.84 IPGGDD2299 pKa = 3.36 FTVGTNGAVIWTPTPDD2315 pKa = 6.28 DD2316 pKa = 3.33 IDD2318 pKa = 3.61 QYY2320 pKa = 11.61 FDD2322 pKa = 4.01 VEE2324 pKa = 4.28 LQVDD2328 pKa = 3.92 DD2329 pKa = 5.01 GNGGSARR2336 pKa = 11.84 QAYY2339 pKa = 9.3 QILVHH2344 pKa = 6.86 ANPANHH2350 pKa = 7.05 DD2351 pKa = 4.48 PIIVSDD2357 pKa = 3.9 PQTEE2361 pKa = 4.11 HH2362 pKa = 7.51 RR2363 pKa = 11.84 LPAPRR2368 pKa = 11.84 NAATGTVSPEE2378 pKa = 4.32 LISLLLEE2385 pKa = 4.27 EE2386 pKa = 5.58 GEE2388 pKa = 4.38 TSIEE2392 pKa = 3.98 PVFYY2396 pKa = 9.84 TSPVGEE2402 pKa = 4.67 LSQATDD2408 pKa = 3.09 VVFIVDD2414 pKa = 3.94 EE2415 pKa = 4.62 SGSMTDD2421 pKa = 2.97 EE2422 pKa = 4.47 HH2423 pKa = 7.1 DD2424 pKa = 3.52 WLGEE2428 pKa = 4.09 MINQLDD2434 pKa = 3.56 SHH2436 pKa = 6.95 LNSEE2440 pKa = 5.01 GISPNYY2446 pKa = 9.13 YY2447 pKa = 10.73 GLVGYY2452 pKa = 10.2 LGTGRR2457 pKa = 11.84 AITNEE2462 pKa = 3.54 EE2463 pKa = 4.35 PYY2465 pKa = 10.43 RR2466 pKa = 11.84 IRR2468 pKa = 11.84 IYY2470 pKa = 11.15 DD2471 pKa = 3.6 NNNVLVQDD2479 pKa = 4.17 LDD2481 pKa = 3.69 INASYY2486 pKa = 10.85 LSPDD2490 pKa = 2.89 IVLPTDD2496 pKa = 4.63 GIYY2499 pKa = 10.39 TIVVDD2504 pKa = 3.87 TEE2506 pKa = 4.2 GAAGLTNTNFILTSITDD2523 pKa = 3.74 EE2524 pKa = 4.22 PVTTSGLDD2532 pKa = 3.25 TTYY2535 pKa = 10.67 SGSITGVEE2543 pKa = 4.12 TVTVNFAASAGQEE2556 pKa = 3.91 IFIDD2560 pKa = 3.77 TTDD2563 pKa = 3.26 VDD2565 pKa = 4.4 DD2566 pKa = 6.57 SIVLYY2571 pKa = 10.64 DD2572 pKa = 3.76 IVHH2575 pKa = 6.45 EE2576 pKa = 4.36 SGKK2579 pKa = 10.1 IAMADD2584 pKa = 2.97 QDD2586 pKa = 3.86 ASVDD2590 pKa = 3.61 SGVFSIPRR2598 pKa = 11.84 SGNYY2602 pKa = 8.83 TLTLKK2607 pKa = 10.11 TSDD2610 pKa = 3.43 GTSTGDD2616 pKa = 3.3 YY2617 pKa = 10.86 AFRR2620 pKa = 11.84 LVDD2623 pKa = 3.84 LTNASTIDD2631 pKa = 3.8 TSWSAGTLSGTLEE2644 pKa = 3.93 TGYY2647 pKa = 10.32 EE2648 pKa = 3.6 AEE2650 pKa = 4.51 YY2651 pKa = 10.16 YY2652 pKa = 10.33 QFAGEE2657 pKa = 4.51 VGDD2660 pKa = 5.1 FLQLDD2665 pKa = 4.04 SRR2667 pKa = 11.84 STGADD2672 pKa = 2.93 TADD2675 pKa = 3.17 ITLISPSGSILLDD2688 pKa = 3.64 GVDD2691 pKa = 3.92 ANSDD2695 pKa = 3.31 AGLTILPEE2703 pKa = 4.16 EE2704 pKa = 4.25 GTYY2707 pKa = 10.84 LVVVSNSSAVSASYY2721 pKa = 10.48 EE2722 pKa = 4.03 FKK2724 pKa = 11.2 LNDD2727 pKa = 3.84 LSTVPTIDD2735 pKa = 3.53 FNTTITGTFDD2745 pKa = 3.12 TVASTNIVKK2754 pKa = 10.08 FDD2756 pKa = 3.62 ASAEE2760 pKa = 4.0 EE2761 pKa = 5.38 LIDD2764 pKa = 3.82 FDD2766 pKa = 3.72 VTARR2770 pKa = 11.84 TGAANATWEE2779 pKa = 4.19 LLSPYY2784 pKa = 9.7 GASLFSGEE2792 pKa = 4.35 FSSASTSDD2800 pKa = 2.99 QTVFATHH2807 pKa = 7.36 DD2808 pKa = 3.34 GTYY2811 pKa = 10.83 YY2812 pKa = 11.43 LLLKK2816 pKa = 10.62 SSPLDD2821 pKa = 3.67 AAGGTYY2827 pKa = 10.15 DD2828 pKa = 4.45 FQMTTQSVTSTGMSLGTLVSDD2849 pKa = 4.76 TISAPGEE2856 pKa = 4.06 YY2857 pKa = 9.89 DD2858 pKa = 3.02 LHH2860 pKa = 6.21 TFTLSEE2866 pKa = 4.15 TKK2868 pKa = 10.26 LVYY2871 pKa = 10.22 FDD2873 pKa = 5.28 SRR2875 pKa = 11.84 TNDD2878 pKa = 3.47 PLLNWSLTGPGGTIVDD2894 pKa = 3.78 RR2895 pKa = 11.84 VRR2897 pKa = 11.84 LSEE2900 pKa = 4.61 ADD2902 pKa = 3.85 GKK2904 pKa = 10.54 YY2905 pKa = 10.59 LPNDD2909 pKa = 3.33 EE2910 pKa = 4.98 AALEE2914 pKa = 4.31 LTAGTYY2920 pKa = 7.8 VVKK2923 pKa = 10.61 IAGNGYY2929 pKa = 8.25 STGDD2933 pKa = 3.41 YY2934 pKa = 10.66 EE2935 pKa = 5.19 FNLVDD2940 pKa = 3.72 LHH2942 pKa = 6.01 TANSAVFNEE2951 pKa = 4.04 NTYY2954 pKa = 9.27 LTLSPGDD2961 pKa = 3.11 RR2962 pKa = 11.84 SAFRR2966 pKa = 11.84 KK2967 pKa = 10.26 FSATAGDD2974 pKa = 3.61 KK2975 pKa = 11.09 LLFEE2979 pKa = 5.58 SISATANRR2987 pKa = 11.84 GYY2989 pKa = 10.08 WRR2991 pKa = 11.84 LIDD2994 pKa = 4.0 PNGEE2998 pKa = 4.09 VVFTRR3003 pKa = 11.84 KK3004 pKa = 10.29 GEE3006 pKa = 3.97 AHH3008 pKa = 6.57 GGYY3011 pKa = 9.91 HH3012 pKa = 6.79 EE3013 pKa = 4.35 LTEE3016 pKa = 4.23 TGIYY3020 pKa = 8.77 TVLIEE3025 pKa = 4.24 GRR3027 pKa = 11.84 GSNTVNFNFNFKK3039 pKa = 8.68 ITPIYY3044 pKa = 8.96 MQGTPLTLGATTSGAISVSGEE3065 pKa = 3.31 HH3066 pKa = 7.5 DD3067 pKa = 3.1 IYY3069 pKa = 11.18 TFTLTQDD3076 pKa = 3.42 ALAHH3080 pKa = 6.42 FDD3082 pKa = 4.37 PLTADD3087 pKa = 3.93 TSLEE3091 pKa = 3.91 WTLFGPNGIVNNQSYY3106 pKa = 10.66 NFAAYY3111 pKa = 10.23 DD3112 pKa = 3.72 DD3113 pKa = 5.9 DD3114 pKa = 4.33 YY3115 pKa = 10.89 HH3116 pKa = 7.1 QHH3118 pKa = 5.58 EE3119 pKa = 4.94 SEE3121 pKa = 4.46 VNSKK3125 pKa = 10.25 VLLDD3129 pKa = 4.51 LLPGNYY3135 pKa = 9.01 TLMIKK3140 pKa = 10.35 DD3141 pKa = 4.19 AGSGTPSYY3149 pKa = 11.4 SFALRR3154 pKa = 11.84 DD3155 pKa = 3.56 LWSEE3159 pKa = 4.42 TISSATVDD3167 pKa = 3.41 GQTINGTLDD3176 pKa = 3.57 PGNGTDD3182 pKa = 4.22 FYY3184 pKa = 10.88 KK3185 pKa = 10.64 ISATAGDD3192 pKa = 3.98 RR3193 pKa = 11.84 LDD3195 pKa = 4.13 FVNSGDD3201 pKa = 3.5 FYY3203 pKa = 11.52 DD3204 pKa = 4.65 YY3205 pKa = 11.07 IRR3207 pKa = 11.84 LLDD3210 pKa = 3.97 PDD3212 pKa = 4.12 GNIVVDD3218 pKa = 4.15 TNANADD3224 pKa = 3.75 FLYY3227 pKa = 10.05 TAEE3230 pKa = 4.14 KK3231 pKa = 9.34 TGDD3234 pKa = 3.61 YY3235 pKa = 10.66 LYY3237 pKa = 11.13 LLEE3240 pKa = 5.38 GDD3242 pKa = 4.76 AYY3244 pKa = 11.25 NSATVSYY3251 pKa = 10.01 SFSVDD3256 pKa = 3.33 LYY3258 pKa = 10.71 VPPGPGGGGSPTVTTSSLTLDD3279 pKa = 3.81 TNVSDD3284 pKa = 4.96 SVATDD3289 pKa = 3.28 EE3290 pKa = 4.32 VNEE3293 pKa = 4.09 YY3294 pKa = 9.76 TFTLGGAKK3302 pKa = 9.6 SLYY3305 pKa = 9.92 FDD3307 pKa = 4.67 ARR3309 pKa = 11.84 TSSADD3314 pKa = 3.67 LSWTLSSTNGGDD3326 pKa = 3.09 IVINRR3331 pKa = 11.84 SFNQSNADD3339 pKa = 4.33 DD3340 pKa = 3.83 ISGLDD3345 pKa = 3.34 VMLNLPADD3353 pKa = 4.7 DD3354 pKa = 3.95 YY3355 pKa = 11.64 TLTISNSGSSTLTYY3369 pKa = 11.02 DD3370 pKa = 4.99 FGLLDD3375 pKa = 4.0 LASATSHH3382 pKa = 7.37 DD3383 pKa = 4.61 INSSSHH3389 pKa = 5.35 VEE3391 pKa = 3.87 YY3392 pKa = 10.86 DD3393 pKa = 3.19 LSTGYY3398 pKa = 8.49 STDD3401 pKa = 2.76 IFSFSATAGDD3411 pKa = 3.43 IFTFSSQDD3419 pKa = 3.3 PGGGGTLDD3427 pKa = 4.26 DD3428 pKa = 4.09 GLLRR3432 pKa = 11.84 VVEE3435 pKa = 4.21 PNGNVVLEE3443 pKa = 4.11 VNYY3446 pKa = 10.64 DD3447 pKa = 3.41 SSHH3450 pKa = 5.79 TGFVAEE3456 pKa = 4.48 TTGTYY3461 pKa = 10.55 YY3462 pKa = 10.26 ILFEE3466 pKa = 6.03 GDD3468 pKa = 3.46 DD3469 pKa = 4.48 SNDD3472 pKa = 2.94 EE3473 pKa = 4.44 TYY3475 pKa = 11.29 EE3476 pKa = 3.74 LTAYY3480 pKa = 8.01 KK3481 pKa = 9.69 TGTGGTVPSPTSITVGQNIDD3501 pKa = 3.5 STISVANEE3509 pKa = 3.02 QDD3511 pKa = 3.77 YY3512 pKa = 11.63 YY3513 pKa = 11.35 EE3514 pKa = 4.2 FTLANDD3520 pKa = 3.37 SLIYY3524 pKa = 10.34 FDD3526 pKa = 5.07 SFSNTSSIRR3535 pKa = 11.84 WDD3537 pKa = 3.48 LASGSGLTLDD3547 pKa = 3.47 NFNYY3551 pKa = 10.27 FSGSDD3556 pKa = 3.47 ANNVSDD3562 pKa = 4.32 AEE3564 pKa = 4.52 VVRR3567 pKa = 11.84 SLKK3570 pKa = 10.79 AGTYY3574 pKa = 8.74 RR3575 pKa = 11.84 IKK3577 pKa = 11.07 VFGSGSSTGDD3587 pKa = 3.07 YY3588 pKa = 11.07 SFTLKK3593 pKa = 10.66 ALSSGTSTSVDD3604 pKa = 3.03 TDD3606 pKa = 3.5 VTGLLDD3612 pKa = 4.0 PANSTNIYY3620 pKa = 9.68 QFSGTAGDD3628 pKa = 4.27 RR3629 pKa = 11.84 LLFDD3633 pKa = 4.13 AQVIGAPTGGKK3644 pKa = 8.2 WRR3646 pKa = 11.84 LIDD3649 pKa = 3.84 PDD3651 pKa = 4.05 GAVLFNTNRR3660 pKa = 11.84 TADD3663 pKa = 3.74 LGVVEE3668 pKa = 4.94 LSKK3671 pKa = 10.06 TGTFYY3676 pKa = 11.65 LLIEE3680 pKa = 4.4 GAVNNSPTGTATYY3693 pKa = 9.73 EE3694 pKa = 3.57 FRR3696 pKa = 11.84 IQSEE3700 pKa = 4.76 VIQTTALTLGTTVSDD3715 pKa = 5.1 AISQIGQKK3723 pKa = 10.07 NEE3725 pKa = 3.67 YY3726 pKa = 9.34 TFTLNSRR3733 pKa = 11.84 TSLYY3737 pKa = 10.69 FDD3739 pKa = 3.47 TLTEE3743 pKa = 4.06 NEE3745 pKa = 3.84 QLYY3748 pKa = 9.46 WKK3750 pKa = 9.11 LTGPKK3755 pKa = 10.37 GEE3757 pKa = 4.32 IVPLTTFSYY3766 pKa = 11.03 EE3767 pKa = 4.0 DD3768 pKa = 4.97 DD3769 pKa = 3.74 YY3770 pKa = 11.91 HH3771 pKa = 7.89 GDD3773 pKa = 3.43 PEE3775 pKa = 4.4 YY3776 pKa = 11.26 DD3777 pKa = 3.36 NRR3779 pKa = 11.84 NLVIDD3784 pKa = 4.04 HH3785 pKa = 6.01 TQVKK3789 pKa = 9.49 PGDD3792 pKa = 3.79 YY3793 pKa = 8.91 TLSVQGTSSGNQYY3806 pKa = 9.15 STGNYY3811 pKa = 9.19 SFKK3814 pKa = 10.96 LSDD3817 pKa = 4.18 LSDD3820 pKa = 3.7 SSSSTVILPGTQVDD3834 pKa = 4.1 QTASPSPANGTDD3846 pKa = 2.68 IYY3848 pKa = 10.99 RR3849 pKa = 11.84 FDD3851 pKa = 3.63 VQAGEE3856 pKa = 4.61 LFHH3859 pKa = 7.1 FDD3861 pKa = 3.65 SVSVTGDD3868 pKa = 3.17 TNGFWRR3874 pKa = 11.84 LIEE3877 pKa = 4.29 PYY3879 pKa = 8.41 GTEE3882 pKa = 3.76 VFEE3885 pKa = 5.37 LSRR3888 pKa = 11.84 NVDD3891 pKa = 3.82 DD3892 pKa = 6.36 GSAADD3897 pKa = 5.07 PIILSIPGTYY3907 pKa = 10.02 YY3908 pKa = 11.44 LLVEE3912 pKa = 4.58 GQAADD3917 pKa = 3.97 TVATDD3922 pKa = 2.97 YY3923 pKa = 11.43 SFNVKK3928 pKa = 10.17 RR3929 pKa = 11.84 IAEE3932 pKa = 4.17 PQLQPSRR3939 pKa = 11.84 DD3940 pKa = 3.78 ALSLNLDD3947 pKa = 3.57 QEE3949 pKa = 4.24 ISGTIDD3955 pKa = 3.08 QASEE3959 pKa = 3.57 PVIYY3963 pKa = 10.57 SFSGTKK3969 pKa = 8.91 GQRR3972 pKa = 11.84 LFYY3975 pKa = 10.77 DD3976 pKa = 4.07 GLSGNSTYY3984 pKa = 10.94 LRR3986 pKa = 11.84 AKK3988 pKa = 9.98 LVQPNGQNLFDD3999 pKa = 4.21 VPANDD4004 pKa = 4.68 DD4005 pKa = 3.7 SGTFTLTHH4013 pKa = 5.56 TGEE4016 pKa = 4.35 YY4017 pKa = 10.31 EE4018 pKa = 4.46 LIIHH4022 pKa = 5.66 STGSTGAFSFRR4033 pKa = 11.84 MVGIDD4038 pKa = 3.19 NTEE4041 pKa = 3.58 IATFDD4046 pKa = 3.55 QEE4048 pKa = 4.53 NVNSISQQNKK4058 pKa = 8.69 SNTFVVNGLEE4068 pKa = 4.13 GDD4070 pKa = 3.75 QFRR4073 pKa = 11.84 ITPGFNFYY4081 pKa = 10.33 MGDD4084 pKa = 3.39 ALDD4087 pKa = 4.14 FQDD4090 pKa = 5.29 RR4091 pKa = 11.84 TDD4093 pKa = 4.15 DD4094 pKa = 3.98 LNTGGGVEE4102 pKa = 4.81 DD4103 pKa = 5.48 GYY4105 pKa = 11.74 AAIDD4109 pKa = 3.56 FALTNFPFRR4118 pKa = 11.84 PGAAVNLVLVTDD4130 pKa = 4.21 EE4131 pKa = 5.35 DD4132 pKa = 4.26 RR4133 pKa = 11.84 DD4134 pKa = 3.91 PFDD4137 pKa = 3.71 VSLSYY4142 pKa = 11.22 SSILADD4148 pKa = 3.85 LEE4150 pKa = 3.99 ARR4152 pKa = 11.84 GAVLHH4157 pKa = 6.43 SVVKK4161 pKa = 10.74 HH4162 pKa = 5.58 NFSTSPYY4169 pKa = 7.66 TGAVIGVDD4177 pKa = 3.92 NEE4179 pKa = 3.79 GMAYY4183 pKa = 9.53 IADD4186 pKa = 3.84 GSGGFTTTSGGTATSGYY4203 pKa = 8.31 GTTKK4207 pKa = 10.6 ADD4209 pKa = 4.17 YY4210 pKa = 10.65 IDD4212 pKa = 5.34 LSWDD4216 pKa = 3.29 NSGVAWNLDD4225 pKa = 3.15 ILRR4228 pKa = 11.84 SGGNSATSFTAAFTEE4243 pKa = 4.09 ILADD4247 pKa = 4.55 EE4248 pKa = 4.71 ITDD4251 pKa = 3.49 QLAIEE4256 pKa = 4.79 VIPSIEE4262 pKa = 3.8 GVTINNLTGVIEE4274 pKa = 4.23 SVLAGEE4280 pKa = 4.45 TVSFDD4285 pKa = 3.57 VEE4287 pKa = 4.39 FTGTTEE4293 pKa = 3.94 AQSFDD4298 pKa = 3.73 LLIVRR4303 pKa = 11.84 KK4304 pKa = 9.55 NDD4306 pKa = 3.01 HH4307 pKa = 7.36 DD4308 pKa = 4.3 YY4309 pKa = 11.36 VLASIPVQINTQYY4322 pKa = 10.71 YY4323 pKa = 8.13 YY4324 pKa = 11.12 NVDD4327 pKa = 4.54 AIDD4330 pKa = 4.49 PDD4332 pKa = 4.15 ADD4334 pKa = 3.28 ILSYY4338 pKa = 10.86 EE4339 pKa = 4.39 LTGEE4343 pKa = 4.13 THH4345 pKa = 6.06 GAEE4348 pKa = 3.9 IDD4350 pKa = 3.54 ADD4352 pKa = 3.88 SGVLVWEE4359 pKa = 4.35 PTAEE4363 pKa = 4.01 GVYY4366 pKa = 10.12 EE4367 pKa = 3.97 FAVLVTDD4374 pKa = 4.46 GNGGKK4379 pKa = 7.99 DD4380 pKa = 3.31 TQTWTVTVGNSQEE4393 pKa = 4.33 EE4394 pKa = 4.11 NTAPIILEE4402 pKa = 4.25 APALTANPGEE4412 pKa = 4.23 SFQYY4416 pKa = 10.44 QVLAEE4421 pKa = 4.66 DD4422 pKa = 4.53 ADD4424 pKa = 4.17 GDD4426 pKa = 4.38 TISYY4430 pKa = 7.66 LTQGVPPEE4438 pKa = 4.21 GLTIDD4443 pKa = 3.46 SHH4445 pKa = 5.96 TGLVEE4450 pKa = 3.8 WTPTIPGEE4458 pKa = 4.22 YY4459 pKa = 9.79 EE4460 pKa = 3.42 FGLRR4464 pKa = 11.84 VSDD4467 pKa = 3.25 RR4468 pKa = 11.84 RR4469 pKa = 11.84 GGVDD4473 pKa = 3.3 VEE4475 pKa = 4.71 TFTITVNEE4483 pKa = 4.08 YY4484 pKa = 9.55 PPANRR4489 pKa = 11.84 RR4490 pKa = 11.84 PIITSTPHH4498 pKa = 5.16 YY4499 pKa = 10.09 SGVSNEE4505 pKa = 3.98 LYY4507 pKa = 10.26 RR4508 pKa = 11.84 YY4509 pKa = 9.2 DD4510 pKa = 4.37 AAAIDD4515 pKa = 4.23 YY4516 pKa = 9.95 DD4517 pKa = 3.85 FEE4519 pKa = 5.22 PIRR4522 pKa = 11.84 FEE4524 pKa = 4.93 LVAGPAGMTIDD4535 pKa = 3.79 EE4536 pKa = 4.34 QTGVLVWNPRR4546 pKa = 11.84 DD4547 pKa = 3.52 KK4548 pKa = 11.21 DD4549 pKa = 3.38 LGYY4552 pKa = 10.11 IPVGIKK4558 pKa = 10.62 VIDD4561 pKa = 3.72 TQGGFDD4567 pKa = 3.32 TQYY4570 pKa = 10.78 FEE4572 pKa = 5.43 IVIHH4576 pKa = 5.97 SLNEE4580 pKa = 3.66 PPEE4583 pKa = 4.4 FVSEE4587 pKa = 4.23 PSPAMGVNQPWSYY4600 pKa = 10.91 QSEE4603 pKa = 4.21 AVDD4606 pKa = 3.71 PNGDD4610 pKa = 3.36 VLAYY4614 pKa = 10.69 SLLQGPAGMTVSSDD4628 pKa = 3.29 GLVNWTPTTEE4638 pKa = 3.49 GSYY4641 pKa = 9.94 RR4642 pKa = 11.84 VRR4644 pKa = 11.84 IQADD4648 pKa = 3.54 DD4649 pKa = 3.57 QRR4651 pKa = 11.84 GGTAYY4656 pKa = 10.48 QEE4658 pKa = 4.16 FTLTITDD4665 pKa = 3.85 NSPTSITSTPEE4676 pKa = 3.38 YY4677 pKa = 8.56 YY4678 pKa = 10.15 TYY4680 pKa = 10.88 INEE4683 pKa = 4.63 DD4684 pKa = 3.34 YY4685 pKa = 9.83 TYY4687 pKa = 9.93 EE4688 pKa = 4.01 ITVDD4692 pKa = 3.97 DD4693 pKa = 5.16 PDD4695 pKa = 3.98 VGDD4698 pKa = 3.95 TVTISLDD4705 pKa = 3.63 DD4706 pKa = 3.82 EE4707 pKa = 4.47 SLARR4711 pKa = 11.84 GMTLTGSTLSWTPDD4725 pKa = 3.03 VLGQFEE4731 pKa = 4.37 VRR4733 pKa = 11.84 ITATDD4738 pKa = 3.24 QDD4740 pKa = 4.24 GAGQAQVYY4748 pKa = 8.43 HH4749 pKa = 5.65 LHH4751 pKa = 5.93 VVEE4754 pKa = 4.34 EE4755 pKa = 4.8 VIRR4758 pKa = 11.84 SQPPIFDD4765 pKa = 3.81 SSPTGPAVVDD4775 pKa = 3.91 EE4776 pKa = 4.34 EE4777 pKa = 4.28 WTYY4780 pKa = 10.94 EE4781 pKa = 4.37 IEE4783 pKa = 4.39 VTDD4786 pKa = 4.06 PDD4788 pKa = 4.02 GDD4790 pKa = 4.0 EE4791 pKa = 4.83 VILSLVSGSFPVGMSLNGTTVSWTPSAVSAGEE4823 pKa = 3.91 LVSIQAEE4830 pKa = 4.35 DD4831 pKa = 3.44 PHH4833 pKa = 8.64 GSATIQTFEE4842 pKa = 4.76 LPVVSTPEE4850 pKa = 3.8 VNNAPVITSIPNVSTPVEE4868 pKa = 3.82 EE4869 pKa = 4.45 EE4870 pKa = 3.45 YY4871 pKa = 10.39 RR4872 pKa = 11.84 YY4873 pKa = 10.3 QIEE4876 pKa = 4.81 AYY4878 pKa = 9.95 DD4879 pKa = 3.98 PNGDD4883 pKa = 3.78 PLSYY4887 pKa = 10.52 SFSVTDD4893 pKa = 4.21 RR4894 pKa = 11.84 VASSIGSSLIDD4905 pKa = 3.78 GSITDD4910 pKa = 3.65 SSLSGGEE4917 pKa = 3.83 ATFSITGPTNVVEE4930 pKa = 4.52 GTDD4933 pKa = 3.5 DD4934 pKa = 3.47 ATFTLSYY4941 pKa = 10.43 TGLLNEE4947 pKa = 4.6 GQTASVDD4954 pKa = 3.77 LAHH4957 pKa = 6.87 VLDD4960 pKa = 4.23 STDD4963 pKa = 3.16 ASDD4966 pKa = 4.47 YY4967 pKa = 11.4 SNSLQAAINDD4977 pKa = 4.61 AISGEE4982 pKa = 4.25 SLISFDD4988 pKa = 3.43 GTTLTFTGASEE4999 pKa = 4.52 DD5000 pKa = 4.0 PLPGSSEE5007 pKa = 4.37 GYY5009 pKa = 10.43 AGAATSITSSFTNWSSPTNATGDD5032 pKa = 3.9 STGSPASISLNSNQPSEE5049 pKa = 4.21 ALAVTDD5055 pKa = 3.67 YY5056 pKa = 11.47 GLNIPANATITGIQLTLVTTGSEE5079 pKa = 3.98 EE5080 pKa = 4.32 TGWEE5084 pKa = 4.3 DD5085 pKa = 4.45 IALTKK5090 pKa = 10.81 NGTSGVSYY5098 pKa = 10.53 KK5099 pKa = 10.9 NSGSSGAWDD5108 pKa = 3.81 GGSIVYY5114 pKa = 10.27 GGSTDD5119 pKa = 3.06 LWGTTWTPAEE5129 pKa = 4.13 INSTNFGSLIVVEE5142 pKa = 5.4 RR5143 pKa = 11.84 YY5144 pKa = 9.97 SGTDD5148 pKa = 2.78 SFYY5151 pKa = 10.94 VYY5153 pKa = 10.34 SAEE5156 pKa = 3.72 IEE5158 pKa = 4.44 VFYY5161 pKa = 7.99 EE5162 pKa = 4.32 TPTIPQITTTDD5173 pKa = 2.69 IDD5175 pKa = 3.88 FTLDD5179 pKa = 3.08 IADD5182 pKa = 4.49 DD5183 pKa = 3.72 ATTEE5187 pKa = 4.25 GNEE5190 pKa = 3.94 QFQITLSNPQTTNASGAALIAGAAATTIIDD5220 pKa = 4.71 DD5221 pKa = 4.26 EE5222 pKa = 4.64 TSSAPIVFNITGGKK5236 pKa = 8.46 NASEE5240 pKa = 3.77 NSPEE5244 pKa = 3.98 NEE5246 pKa = 3.54 LDD5248 pKa = 3.38 YY5249 pKa = 11.21 TIGYY5253 pKa = 7.62 TGALTAGDD5261 pKa = 3.43 IAQINVLHH5269 pKa = 6.44 NLNEE5273 pKa = 4.51 TTVSDD5278 pKa = 3.84 YY5279 pKa = 11.65 DD5280 pKa = 3.82 VDD5282 pKa = 4.07 VVTAIQNAAAATDD5295 pKa = 3.89 GVLFDD5300 pKa = 4.01 GTTLTFFGGNDD5311 pKa = 3.63 YY5312 pKa = 10.72 PSSIDD5317 pKa = 3.67 FSVVLADD5324 pKa = 4.1 DD5325 pKa = 4.82 AEE5327 pKa = 4.54 LEE5329 pKa = 4.39 STEE5332 pKa = 4.27 SFSIEE5337 pKa = 3.86 LSQTTGNTSITGLDD5351 pKa = 3.65 FTEE5354 pKa = 4.34 EE5355 pKa = 5.12 GILTWTPDD5363 pKa = 3.59 FEE5365 pKa = 5.03 SSIDD5369 pKa = 3.74 VEE5371 pKa = 4.39 ITVSDD5376 pKa = 3.73 GRR5378 pKa = 11.84 GGSATQTYY5386 pKa = 8.31 TLTSYY5391 pKa = 10.67 IPNTPPDD5398 pKa = 3.64 ISSVPSGPAVVDD5410 pKa = 4.79 DD5411 pKa = 3.78 EE5412 pKa = 4.47 WTYY5415 pKa = 11.53 QLDD5418 pKa = 3.75 AFDD5421 pKa = 4.53 IDD5423 pKa = 4.42 EE5424 pKa = 5.32 DD5425 pKa = 4.14 SLSYY5429 pKa = 11.22 ALNASALSSGFTIDD5443 pKa = 4.61 ANGLVSWTPTAVGSEE5458 pKa = 3.96 AVEE5461 pKa = 3.97 ITVSDD5466 pKa = 3.62 GRR5468 pKa = 11.84 GGIAIQSFTMNAIAEE5483 pKa = 4.47 HH5484 pKa = 6.07 TLNHH5488 pKa = 6.41 APVITSVPEE5497 pKa = 4.1 GPAEE5501 pKa = 4.29 VGSTWTYY5508 pKa = 11.15 NIVATDD5514 pKa = 3.72 ADD5516 pKa = 5.16 LDD5518 pKa = 3.93 PLNYY5522 pKa = 9.42 FVSVPAPEE5530 pKa = 4.61 APSVVVASIAGSIVDD5545 pKa = 3.9 NQSTTSSEE5553 pKa = 3.74 ATFAISGDD5561 pKa = 3.48 ATVTEE5566 pKa = 5.08 DD5567 pKa = 5.08 PNDD5570 pKa = 3.88 ADD5572 pKa = 4.75 GNTANYY5578 pKa = 9.8 QVTFTSSLASTEE5590 pKa = 4.16 TASVDD5595 pKa = 2.8 ISQFLGDD5602 pKa = 3.76 TTSGDD5607 pKa = 3.5 FSEE5610 pKa = 4.62 TMISALTTAANAEE5623 pKa = 4.4 SSVSLSGSTLTFQGNGTVSSSAEE5646 pKa = 3.95 EE5647 pKa = 4.18 EE5648 pKa = 4.09 FATAAVADD5656 pKa = 3.98 VGAGSEE5662 pKa = 3.84 EE5663 pKa = 4.19 WANVSYY5669 pKa = 10.74 AADD5672 pKa = 3.98 GNEE5675 pKa = 3.82 STAAHH5680 pKa = 6.29 AVLDD5684 pKa = 4.24 KK5685 pKa = 11.82 YY5686 pKa = 10.7 EE5687 pKa = 4.67 FPDD5690 pKa = 3.97 PLQLTDD5696 pKa = 4.04 YY5697 pKa = 10.55 DD5698 pKa = 4.3 FSIPSTATIEE5708 pKa = 4.76 GIKK5711 pKa = 9.27 VTVNTSGSNNPMTLTSPIAITKK5733 pKa = 10.4 DD5734 pKa = 3.23 GTSDD5738 pKa = 3.09 AGTPGTTTGTWSGGTVVYY5756 pKa = 10.46 GGSSDD5761 pKa = 3.59 LWGTTWTAADD5771 pKa = 3.61 INSSNFGVIVAPQGSNNGPDD5791 pKa = 3.91 YY5792 pKa = 10.38 YY5793 pKa = 11.19 VHH5795 pKa = 6.3 SAKK5798 pKa = 10.15 IEE5800 pKa = 3.75 ITYY5803 pKa = 10.64 EE5804 pKa = 4.07 SVTGDD5809 pKa = 3.36 GTTVLQFSLPVTNDD5823 pKa = 2.92 SEE5825 pKa = 4.74 TEE5827 pKa = 3.82 EE5828 pKa = 4.35 SEE5830 pKa = 5.18 AYY5832 pKa = 10.15 ALQLSNAQTANTDD5845 pKa = 3.15 GVLITQNTTSTTIVDD5860 pKa = 4.54 DD5861 pKa = 4.01 EE5862 pKa = 4.55 TTAAPIVFSITGDD5875 pKa = 3.2 TSTTEE5880 pKa = 3.78 NTGDD5884 pKa = 3.38 VDD5886 pKa = 4.17 NNEE5889 pKa = 3.72 ATYY5892 pKa = 9.41 TISYY5896 pKa = 8.66 TGTLAEE5902 pKa = 4.53 GEE5904 pKa = 4.4 VTRR5907 pKa = 11.84 IDD5909 pKa = 3.81 VVHH5912 pKa = 6.19 VLNDD5916 pKa = 3.65 TASADD5921 pKa = 3.9 YY5922 pKa = 9.28 ITDD5925 pKa = 3.14 VVTALTSAAAATSGVDD5941 pKa = 4.12 FDD5943 pKa = 4.16 GTTLTFYY5950 pKa = 11.14 GGGSNPNSIDD5960 pKa = 3.47 FTVVLSDD5967 pKa = 4.08 DD5968 pKa = 3.77 ATSEE5972 pKa = 4.07 STEE5975 pKa = 3.92 SYY5977 pKa = 10.24 RR5978 pKa = 11.84 IEE5980 pKa = 4.07 LHH5982 pKa = 4.84 TTEE5985 pKa = 4.62 SPYY5988 pKa = 10.98 VPIGMNLTSGGVLTWDD6004 pKa = 3.4 STAEE6008 pKa = 4.04 TEE6010 pKa = 4.2 LDD6012 pKa = 3.14 ILITVDD6018 pKa = 4.05 DD6019 pKa = 4.38 GRR6021 pKa = 11.84 GGYY6024 pKa = 7.84 ATQSFTLSSVDD6035 pKa = 3.53 PNTPPGITSVPTGPAYY6051 pKa = 10.67 VDD6053 pKa = 3.73 EE6054 pKa = 4.22 QWTYY6058 pKa = 9.13 QVEE6061 pKa = 4.37 AEE6063 pKa = 4.49 DD6064 pKa = 5.25 LEE6066 pKa = 4.54 QATLDD6071 pKa = 3.84 FDD6073 pKa = 5.23 LDD6075 pKa = 3.76 AASVSNGFAIDD6086 pKa = 3.84 SNGLITWTPTAVTSEE6101 pKa = 4.35 TVEE6104 pKa = 4.11 VTVTDD6109 pKa = 4.19 GNGGSGTQTFMIDD6122 pKa = 3.57 AIVRR6126 pKa = 11.84 STIDD6130 pKa = 3.35 NAPTITSTPEE6140 pKa = 3.98 GPGLTEE6146 pKa = 4.27 TLWSYY6151 pKa = 11.14 QVTATDD6157 pKa = 4.08 PNEE6160 pKa = 4.47 DD6161 pKa = 4.18 PLTFSLDD6168 pKa = 3.25 QDD6170 pKa = 3.9 SLNRR6174 pKa = 11.84 GMTITDD6180 pKa = 4.07 GLVEE6184 pKa = 4.21 WTPNTIGGYY6193 pKa = 9.2 PIIVSVDD6200 pKa = 3.48 DD6201 pKa = 4.1 GNGNVVTQSFTLQVIDD6217 pKa = 4.5 SSATNSPPAINSSPQGPAYY6236 pKa = 10.65 LNNEE6240 pKa = 3.12 WSYY6243 pKa = 11.47 QVDD6246 pKa = 3.58 ATDD6249 pKa = 4.68 PDD6251 pKa = 4.99 LDD6253 pKa = 4.15 PLTYY6257 pKa = 10.82 SLDD6260 pKa = 3.95 SAGLAAGLQISAGGLITWTPTVEE6283 pKa = 4.16 TEE6285 pKa = 3.48 ITAVITVEE6293 pKa = 4.16 DD6294 pKa = 4.19 GKK6296 pKa = 10.88 AAYY6299 pKa = 7.49 ATQTFTLASINEE6311 pKa = 4.3 PPPSNDD6317 pKa = 3.02 PPVISSVPGNQVKK6330 pKa = 10.72 SGDD6333 pKa = 3.53 LYY6335 pKa = 11.01 LYY6337 pKa = 10.06 QVSAYY6342 pKa = 10.11 DD6343 pKa = 3.71 PNGDD6347 pKa = 3.6 ALIYY6351 pKa = 10.64 SLVTAPSSMTISSSGLISWTAGDD6374 pKa = 4.11 ADD6376 pKa = 4.26 TYY6378 pKa = 10.27 SVSVKK6383 pKa = 10.52 VEE6385 pKa = 4.13 DD6386 pKa = 3.53 SSGASVTQTYY6396 pKa = 7.95 TLHH6399 pKa = 7.17 VLEE6402 pKa = 4.96 EE6403 pKa = 4.18 RR6404 pKa = 11.84 PPLEE6408 pKa = 4.27 EE6409 pKa = 4.48 NEE6411 pKa = 4.38 PPVITSTPTGPTALNRR6427 pKa = 11.84 GYY6429 pKa = 10.37 QYY6431 pKa = 9.84 QTIASDD6437 pKa = 3.5 ANQDD6441 pKa = 3.77 VITFSLDD6448 pKa = 3.19 QNSLGRR6454 pKa = 11.84 GATIDD6459 pKa = 3.81 SEE6461 pKa = 4.58 TGLIEE6466 pKa = 3.95 WTPITAGTFEE6476 pKa = 4.35 FTVTATDD6483 pKa = 3.68 PFGASDD6489 pKa = 3.81 SQTFSLPVVDD6499 pKa = 5.15 NAPPYY6504 pKa = 9.16 ITSKK6508 pKa = 9.93 PDD6510 pKa = 2.69 QYY6512 pKa = 11.27 TDD6514 pKa = 2.86 MDD6516 pKa = 3.75 VEE6518 pKa = 4.07 YY6519 pKa = 8.81 TYY6521 pKa = 11.51 AVTAQDD6527 pKa = 4.6 PNPGDD6532 pKa = 3.78 TITYY6536 pKa = 9.63 SLSSPLSGMSIDD6548 pKa = 3.7 AQTGQFSWTPSSIGVFRR6565 pKa = 11.84 VNIIVTDD6572 pKa = 3.73 NVGASSGQSFDD6583 pKa = 5.04 LVVSDD6588 pKa = 5.35 PSTPNQNPSIISSPRR6603 pKa = 11.84 TSIQLGTSFVHH6614 pKa = 6.23 QVIATDD6620 pKa = 4.27 PDD6622 pKa = 4.26 FDD6624 pKa = 4.45 DD6625 pKa = 5.43 LNYY6628 pKa = 11.05 SLLASAPGGMVIDD6641 pKa = 3.99 NGGLITWTPDD6651 pKa = 3.17 GEE6653 pKa = 4.67 DD6654 pKa = 3.52 VATSPHH6660 pKa = 6.74 SYY6662 pKa = 8.43 TVRR6665 pKa = 11.84 VEE6667 pKa = 4.24 DD6668 pKa = 3.42 GHH6670 pKa = 8.47 GGFTEE6675 pKa = 3.88 QTYY6678 pKa = 10.69 LLSVVTSEE6686 pKa = 4.46 VNSAPAITSTPSVNATTGLGYY6707 pKa = 10.32 DD6708 pKa = 3.81 YY6709 pKa = 10.82 QAAATDD6715 pKa = 4.12 PEE6717 pKa = 5.41 ADD6719 pKa = 3.53 SLVWNLNNGPTGMQIDD6735 pKa = 4.4 SEE6737 pKa = 4.5 TGYY6740 pKa = 11.16 LSWSPNLNQIGQHH6753 pKa = 5.87 DD6754 pKa = 4.36 VSLTVTDD6761 pKa = 4.15 TYY6763 pKa = 11.46 GGNSTQTYY6771 pKa = 7.65 TLTVRR6776 pKa = 11.84 SSNLAPMIDD6785 pKa = 3.44 SFPITEE6791 pKa = 5.41 AIANTAYY6798 pKa = 10.51 YY6799 pKa = 9.09 YY6800 pKa = 10.67 QVDD6803 pKa = 4.12 AYY6805 pKa = 10.78 DD6806 pKa = 4.26 PDD6808 pKa = 4.32 GDD6810 pKa = 4.31 EE6811 pKa = 4.07 ITYY6814 pKa = 11.01 SLAAYY6819 pKa = 8.51 PSGMTINATSGMINWTPAGSGSVNVIVRR6847 pKa = 11.84 VTDD6850 pKa = 3.34 ARR6852 pKa = 11.84 GASVDD6857 pKa = 3.35 QSYY6860 pKa = 8.34 TLVVAAPATNEE6871 pKa = 3.87 PPQFASTPPSLALVGTLYY6889 pKa = 9.75 THH6891 pKa = 7.81 ALQTTDD6897 pKa = 4.05 PNNDD6901 pKa = 2.9 TVTFEE6906 pKa = 4.68 LLNGPTSMSIDD6917 pKa = 3.67 TNTGLIEE6924 pKa = 4.06 WTPASASSNWEE6935 pKa = 3.99 PIRR6938 pKa = 11.84 IRR6940 pKa = 11.84 ISDD6943 pKa = 3.54 GTYY6946 pKa = 9.84 LVSKK6950 pKa = 8.68 TFYY6953 pKa = 10.6 VKK6955 pKa = 10.57 ARR6957 pKa = 11.84 ANSAPAIAAINDD6969 pKa = 3.51 KK6970 pKa = 10.09 TISAGKK6976 pKa = 9.39 SFRR6979 pKa = 11.84 VDD6981 pKa = 3.33 VNASDD6986 pKa = 4.67 PDD6988 pKa = 3.9 GDD6990 pKa = 4.52 PIFYY6994 pKa = 10.6 SLTTAPDD7001 pKa = 3.54 GATVDD7006 pKa = 3.56 DD7007 pKa = 5.15 FGRR7010 pKa = 11.84 ILWTTDD7016 pKa = 2.64 SSDD7019 pKa = 3.51 VGTHH7023 pKa = 6.17 PVTVTVTDD7031 pKa = 4.05 LLGVSTSEE7039 pKa = 4.18 TFDD7042 pKa = 3.0 ITVNADD7048 pKa = 3.45 TEE7050 pKa = 4.86 VPFVNLYY7057 pKa = 10.17 IDD7059 pKa = 4.19 TYY7061 pKa = 11.17 PNGNLGTEE7069 pKa = 4.18 VRR7071 pKa = 11.84 LQLYY7075 pKa = 10.8 ANDD7078 pKa = 3.58 NVAIASKK7085 pKa = 9.04 TLNLASITVGGTTTPINRR7103 pKa = 11.84 VLVLDD7108 pKa = 4.04 SNGWTTLTLDD7118 pKa = 4.82 QIGTLNFEE7126 pKa = 4.13 GTAVDD7131 pKa = 3.6 LAGNVGTATPISLQVVDD7148 pKa = 4.84 PADD7151 pKa = 3.61 VLPPSVNILLPDD7163 pKa = 3.36 SDD7165 pKa = 5.77 AILTEE7170 pKa = 4.05 PTDD7173 pKa = 3.82 VIGSVDD7179 pKa = 3.99 DD7180 pKa = 5.62 DD7181 pKa = 3.98 IPASVSWTLTLNSLDD7196 pKa = 3.53 GSISKK7201 pKa = 10.16 EE7202 pKa = 3.88 LGTGTGALSNAVLGEE7217 pKa = 3.9 IDD7219 pKa = 3.79 TTLLPNGTYY7228 pKa = 11.13 ALTLDD7233 pKa = 3.91 AVDD7236 pKa = 4.6 NGAHH7240 pKa = 6.07 SASEE7244 pKa = 3.88 TTYY7247 pKa = 10.75 IEE7249 pKa = 3.81 IEE7251 pKa = 4.21 GALKK7255 pKa = 10.63 LGNFSVSFLDD7265 pKa = 3.88 LAVPVAGIPITITRR7279 pKa = 11.84 TYY7281 pKa = 10.0 DD7282 pKa = 3.03 TLNANKK7288 pKa = 10.05 QGDD7291 pKa = 3.91 FGYY7294 pKa = 10.77 GWNLDD7299 pKa = 3.38 ISNVKK7304 pKa = 10.05 VDD7306 pKa = 3.23 VDD7308 pKa = 4.05 YY7309 pKa = 11.77 VSGVPQEE7316 pKa = 4.36 SSLGDD7321 pKa = 4.08 DD7322 pKa = 3.51 IYY7324 pKa = 11.46 YY7325 pKa = 9.75 PQDD7328 pKa = 3.05 TRR7330 pKa = 11.84 IVITMPDD7337 pKa = 3.41 GSTEE7341 pKa = 4.04 GFTLYY7346 pKa = 10.27 YY7347 pKa = 10.87 APDD7350 pKa = 4.28 AEE7352 pKa = 4.55 TAIPIYY7358 pKa = 10.81 GPMYY7362 pKa = 9.38 WIPKK7366 pKa = 7.82 FQSDD7370 pKa = 4.35 SGNQTEE7376 pKa = 4.5 LVVPEE7381 pKa = 4.52 ILLQQVEE7388 pKa = 4.52 GEE7390 pKa = 4.3 MYY7392 pKa = 10.89 LPGTLTGYY7400 pKa = 10.75 NPTLSEE7406 pKa = 4.14 FGGGFTLKK7414 pKa = 10.26 YY7415 pKa = 10.53 RR7416 pKa = 11.84 NDD7418 pKa = 3.45 AEE7420 pKa = 4.55 LFIDD7424 pKa = 5.37 GDD7426 pKa = 4.32 DD7427 pKa = 4.38 LQLSTIKK7434 pKa = 10.98 DD7435 pKa = 3.24 SDD7437 pKa = 3.79 GNKK7440 pKa = 10.25 LFFTEE7445 pKa = 4.33 TGITSDD7451 pKa = 3.08 SGRR7454 pKa = 11.84 GIEE7457 pKa = 4.98 FEE7459 pKa = 3.82 RR7460 pKa = 11.84 DD7461 pKa = 2.81 QAGRR7465 pKa = 11.84 IKK7467 pKa = 10.84 AVIDD7471 pKa = 3.54 PLGNRR7476 pKa = 11.84 ITYY7479 pKa = 8.09 TYY7481 pKa = 10.0 NANGDD7486 pKa = 4.65 LIAVTDD7492 pKa = 3.94 RR7493 pKa = 11.84 VEE7495 pKa = 3.84 ATTQFTYY7502 pKa = 10.83 LDD7504 pKa = 3.77 EE7505 pKa = 6.55 SGDD7508 pKa = 3.62 PAHH7511 pKa = 6.65 YY7512 pKa = 9.83 LDD7514 pKa = 6.21 EE7515 pKa = 6.19 IIAPNGLPAARR7526 pKa = 11.84 SEE7528 pKa = 3.95 YY7529 pKa = 10.91 DD7530 pKa = 3.01 EE7531 pKa = 4.41 TGRR7534 pKa = 11.84 LTKK7537 pKa = 10.74 VIDD7540 pKa = 3.94 ADD7542 pKa = 3.87 GQTILLDD7549 pKa = 3.63 YY7550 pKa = 11.27 DD7551 pKa = 3.79 SDD7553 pKa = 3.91 LKK7555 pKa = 11.36 KK7556 pKa = 10.46 EE7557 pKa = 4.06 VRR7559 pKa = 11.84 TDD7561 pKa = 3.32 QLDD7564 pKa = 4.36 EE7565 pKa = 4.51 IWTTWYY7571 pKa = 10.25 DD7572 pKa = 3.5 DD7573 pKa = 3.56 NGNVIRR7579 pKa = 11.84 TEE7581 pKa = 4.2 DD7582 pKa = 3.84 PLGGIVIMTYY7592 pKa = 11.06 NDD7594 pKa = 3.8 DD7595 pKa = 4.47 DD7596 pKa = 4.47 LVLSEE7601 pKa = 4.11 TRR7603 pKa = 11.84 VIGSEE7608 pKa = 3.76 DD7609 pKa = 3.5 DD7610 pKa = 3.94 GVNGEE7615 pKa = 4.23 TDD7617 pKa = 4.01 DD7618 pKa = 3.61 LTTYY7622 pKa = 8.63 YY7623 pKa = 10.02 TYY7625 pKa = 11.08 KK7626 pKa = 10.98 ANGDD7630 pKa = 3.7 PRR7632 pKa = 11.84 TVTDD7636 pKa = 3.27 IFGNTSQSFYY7646 pKa = 11.24 NPDD7649 pKa = 2.98 GFLYY7653 pKa = 10.35 QQVDD7657 pKa = 3.37 TFGNVTRR7664 pKa = 11.84 YY7665 pKa = 9.44 SQSPYY7670 pKa = 10.89 GSGNKK7675 pKa = 9.04 QSVTDD7680 pKa = 3.87 PFGNTTTITQTADD7693 pKa = 2.96 GGGNRR7698 pKa = 11.84 YY7699 pKa = 10.0 DD7700 pKa = 5.36 LIEE7703 pKa = 4.66 DD7704 pKa = 3.32 QNGNITRR7711 pKa = 11.84 IDD7713 pKa = 3.63 YY7714 pKa = 10.88 NSYY7717 pKa = 10.71 GDD7719 pKa = 3.77 VIKK7722 pKa = 10.03 TSGSLGSPVTFTIDD7736 pKa = 4.23 DD7737 pKa = 4.24 LGNQIGTSVIWVNPDD7752 pKa = 2.95 NPTDD7756 pKa = 3.43 THH7758 pKa = 5.97 TLSSSTEE7765 pKa = 3.67 YY7766 pKa = 10.87 DD7767 pKa = 3.24 DD7768 pKa = 3.82 QGRR7771 pKa = 11.84 VFHH7774 pKa = 6.1 TTDD7777 pKa = 2.99 AQGNEE7782 pKa = 3.4 SWTNYY7787 pKa = 9.88 NNIGQVASTTNDD7799 pKa = 3.26 LGQSTYY7805 pKa = 11.25 YY7806 pKa = 10.75 LYY7808 pKa = 10.53 DD7809 pKa = 3.09 ARR7811 pKa = 11.84 GQVVMSINHH7820 pKa = 6.93 DD7821 pKa = 3.17 GSYY7824 pKa = 10.08 SQTVFDD7830 pKa = 4.09 AKK7832 pKa = 10.77 GRR7834 pKa = 11.84 STHH7837 pKa = 6.14 SASTNYY7843 pKa = 10.54 AGLSRR7848 pKa = 11.84 GGSHH7852 pKa = 7.54 SIYY7855 pKa = 10.39 DD7856 pKa = 3.5 ALDD7859 pKa = 3.34 RR7860 pKa = 11.84 VITSEE7865 pKa = 4.51 SRR7867 pKa = 11.84 DD7868 pKa = 3.7 DD7869 pKa = 3.63 LTIQLVDD7876 pKa = 3.65 ANEE7879 pKa = 3.96 VAFNVSINAQGKK7891 pKa = 9.92 LIDD7894 pKa = 4.81 DD7895 pKa = 4.05 ATSLEE7900 pKa = 4.0 VDD7902 pKa = 3.87 LDD7904 pKa = 4.56 AIFGTIVTNAGTVTDD7919 pKa = 4.11 SSSTVYY7925 pKa = 10.88 DD7926 pKa = 4.57 PISGRR7931 pKa = 11.84 MLSTSNFDD7939 pKa = 3.83 GDD7941 pKa = 3.96 TTTYY7945 pKa = 10.36 EE7946 pKa = 4.29 YY7947 pKa = 11.22 NEE7949 pKa = 4.64 KK7950 pKa = 9.95 SQQSAVEE7957 pKa = 4.32 SVSDD7961 pKa = 4.6 GITRR7965 pKa = 11.84 RR7966 pKa = 11.84 SEE7968 pKa = 3.88 TEE7970 pKa = 3.29 YY7971 pKa = 11.34 DD7972 pKa = 3.36 EE7973 pKa = 5.41 FGRR7976 pKa = 11.84 TYY7978 pKa = 9.99 IQRR7981 pKa = 11.84 QFVVDD7986 pKa = 4.78 LNNPTADD7993 pKa = 3.45 PEE7995 pKa = 4.08 IRR7997 pKa = 11.84 EE7998 pKa = 4.27 TIYY8001 pKa = 10.71 EE8002 pKa = 3.85 YY8003 pKa = 11.25 DD8004 pKa = 3.09 EE8005 pKa = 4.8 FGRR8008 pKa = 11.84 QFRR8011 pKa = 11.84 TTDD8014 pKa = 2.97 YY8015 pKa = 10.14 HH8016 pKa = 7.33 GRR8018 pKa = 11.84 VSTTTYY8024 pKa = 10.98 DD8025 pKa = 3.31 DD8026 pKa = 3.23 QGRR8029 pKa = 11.84 VDD8031 pKa = 3.63 TQTSYY8036 pKa = 11.71 NGTVTKK8042 pKa = 10.47 NYY8044 pKa = 8.26 YY8045 pKa = 10.52 DD8046 pKa = 3.22 NYY8048 pKa = 8.48 GRR8050 pKa = 11.84 QYY8052 pKa = 9.54 KK8053 pKa = 9.64 TDD8055 pKa = 3.37 VTVGTEE8061 pKa = 3.68 TRR8063 pKa = 11.84 TTEE8066 pKa = 3.78 TVYY8069 pKa = 11.14 DD8070 pKa = 4.01 SNGNRR8075 pKa = 11.84 AQNIDD8080 pKa = 3.49 PLGRR8084 pKa = 11.84 ITMYY8088 pKa = 10.76 AYY8090 pKa = 10.26 DD8091 pKa = 3.36 QFGRR8095 pKa = 11.84 QVSMTQVVGGIDD8107 pKa = 3.59 GSAGHH8112 pKa = 6.36 EE8113 pKa = 4.3 SEE8115 pKa = 4.45 TDD8117 pKa = 3.38 DD8118 pKa = 5.51 LATQTFYY8125 pKa = 11.4 DD8126 pKa = 3.24 VDD8128 pKa = 3.84 GNIDD8132 pKa = 3.55 YY8133 pKa = 10.04 EE8134 pKa = 4.37 INQLGLTRR8142 pKa = 11.84 DD8143 pKa = 3.65 YY8144 pKa = 11.13 EE8145 pKa = 4.21 YY8146 pKa = 11.04 DD8147 pKa = 3.34 AEE8149 pKa = 4.39 GRR8151 pKa = 11.84 LTGVVLPQVYY8161 pKa = 10.49 DD8162 pKa = 3.63 PVSQQTISPRR8172 pKa = 11.84 YY8173 pKa = 7.76 VYY8175 pKa = 10.83 VYY8177 pKa = 10.32 DD8178 pKa = 3.53 DD8179 pKa = 4.7 HH8180 pKa = 7.55 GNQTEE8185 pKa = 3.9 IHH8187 pKa = 7.03 EE8188 pKa = 4.53 YY8189 pKa = 9.75 TSATVFRR8196 pKa = 11.84 KK8197 pKa = 7.21 TTFTYY8202 pKa = 10.74 NDD8204 pKa = 3.08 YY8205 pKa = 10.79 GQRR8208 pKa = 11.84 LTRR8211 pKa = 11.84 EE8212 pKa = 4.2 LPSGEE8217 pKa = 4.01 IEE8219 pKa = 4.32 YY8220 pKa = 8.47 FTYY8223 pKa = 10.5 DD8224 pKa = 3.19 VEE8226 pKa = 4.68 EE8227 pKa = 4.27 YY8228 pKa = 10.67 RR8229 pKa = 11.84 QDD8231 pKa = 2.96 THH8233 pKa = 7.07 TDD8235 pKa = 3.64 FEE8237 pKa = 5.12 GNIVDD8242 pKa = 4.58 SVFNADD8248 pKa = 5.08 DD8249 pKa = 3.89 FLEE8252 pKa = 4.1 QMLYY8256 pKa = 7.99 FTPGSDD8262 pKa = 3.44 PAVDD8266 pKa = 4.29 APAEE8270 pKa = 4.27 TVTYY8274 pKa = 10.13 GYY8276 pKa = 10.78 DD8277 pKa = 3.16 DD8278 pKa = 4.15 YY8279 pKa = 11.84 GRR8281 pKa = 11.84 KK8282 pKa = 9.17 ISMTDD8287 pKa = 2.91 NRR8289 pKa = 11.84 GTTTYY8294 pKa = 10.67 SYY8296 pKa = 11.35 DD8297 pKa = 3.34 SNGRR8301 pKa = 11.84 QNRR8304 pKa = 11.84 IEE8306 pKa = 4.11 SPEE8309 pKa = 4.18 GIINYY8314 pKa = 9.06 EE8315 pKa = 3.85 FDD8317 pKa = 5.57 PITGQKK8323 pKa = 8.51 TRR8325 pKa = 11.84 TTTEE8329 pKa = 3.89 EE8330 pKa = 3.69 IGTANITNDD8339 pKa = 2.99 FRR8341 pKa = 11.84 YY8342 pKa = 8.59 TYY8344 pKa = 10.77 DD8345 pKa = 2.83 DD8346 pKa = 3.23 WGRR8349 pKa = 11.84 LKK8351 pKa = 9.94 TVEE8354 pKa = 3.91 VHH8356 pKa = 5.45 EE8357 pKa = 4.43 QNDD8360 pKa = 3.76 VVLVNPEE8367 pKa = 3.87 VTTYY8371 pKa = 10.74 QYY8373 pKa = 11.85 DD8374 pKa = 3.52 LVGNLTYY8381 pKa = 10.78 TFLTNGTITAYY8392 pKa = 9.98 EE8393 pKa = 3.84 YY8394 pKa = 11.2 DD8395 pKa = 3.42 EE8396 pKa = 5.65 LYY8398 pKa = 10.94 RR8399 pKa = 11.84 LDD8401 pKa = 5.61 LLTHH8405 pKa = 6.39 YY8406 pKa = 10.73 YY8407 pKa = 11.22 GDD8409 pKa = 3.91 GDD8411 pKa = 4.59 NINDD8415 pKa = 3.68 TDD8417 pKa = 3.46 MSDD8420 pKa = 3.35 NVKK8423 pKa = 9.48 MAEE8426 pKa = 3.58 YY8427 pKa = 9.74 DD8428 pKa = 3.6 YY8429 pKa = 11.07 TVRR8432 pKa = 11.84 ADD8434 pKa = 3.34 GRR8436 pKa = 11.84 RR8437 pKa = 11.84 TGVTEE8442 pKa = 4.19 STWQSDD8448 pKa = 3.59 EE8449 pKa = 4.17 TLITTDD8455 pKa = 3.13 IFSWTYY8461 pKa = 11.57 DD8462 pKa = 3.17 NLGRR8466 pKa = 11.84 MTQEE8470 pKa = 4.21 VLDD8473 pKa = 4.16 STDD8476 pKa = 3.72 DD8477 pKa = 3.82 SLDD8480 pKa = 3.28 FTATYY8485 pKa = 10.24 TFDD8488 pKa = 4.12 LLGNRR8493 pKa = 11.84 LTKK8496 pKa = 10.81 DD8497 pKa = 3.13 LDD8499 pKa = 3.67 YY8500 pKa = 10.94 FADD8503 pKa = 3.67 DD8504 pKa = 3.83 TRR8506 pKa = 11.84 DD8507 pKa = 3.29 EE8508 pKa = 4.77 VITYY8512 pKa = 7.58 TYY8514 pKa = 11.33 NSANQLTSEE8523 pKa = 4.32 EE8524 pKa = 4.51 KK8525 pKa = 10.32 VVGTVDD8531 pKa = 2.98 VGTAVNDD8538 pKa = 3.22 KK8539 pKa = 9.12 HH8540 pKa = 5.88 TVYY8543 pKa = 10.62 SYY8545 pKa = 10.84 TGTLRR8550 pKa = 11.84 TGKK8553 pKa = 7.92 TVYY8556 pKa = 10.72 NKK8558 pKa = 9.84 VDD8560 pKa = 3.96 FEE8562 pKa = 4.61 TLSFPDD8568 pKa = 4.16 PLEE8571 pKa = 4.35 HH8572 pKa = 6.42 TTFEE8576 pKa = 4.47 YY8577 pKa = 11.0 NLQGRR8582 pKa = 11.84 MGKK8585 pKa = 8.22 VTVDD8589 pKa = 3.49 TYY8591 pKa = 10.62 TGSSITKK8598 pKa = 9.87 QEE8600 pKa = 4.02 VTEE8603 pKa = 4.0 YY8604 pKa = 10.2 EE8605 pKa = 4.16 YY8606 pKa = 11.46 NEE8608 pKa = 3.84 RR8609 pKa = 11.84 GIRR8612 pKa = 11.84 TEE8614 pKa = 3.54 AHH8616 pKa = 5.52 HH8617 pKa = 6.58 TVDD8620 pKa = 3.74 EE8621 pKa = 4.95 GNDD8624 pKa = 3.35 SVLEE8628 pKa = 3.99 VDD8630 pKa = 3.87 EE8631 pKa = 4.47 TTTYY8635 pKa = 11.39 LNDD8638 pKa = 3.83 PNNFTGYY8645 pKa = 9.6 NQVLEE8650 pKa = 4.24 EE8651 pKa = 3.98 VTYY8654 pKa = 11.36 DD8655 pKa = 3.55 NLAEE8659 pKa = 4.16 AEE8661 pKa = 4.26 KK8662 pKa = 11.07 DD8663 pKa = 3.29 RR8664 pKa = 11.84 AIYY8667 pKa = 8.58 TLGMDD8672 pKa = 4.36 IISQFRR8678 pKa = 11.84 VNSSYY8683 pKa = 10.78 PAGFDD8688 pKa = 3.59 TVFHH8692 pKa = 6.7 YY8693 pKa = 10.61 DD8694 pKa = 3.11 GHH8696 pKa = 6.73 GSTRR8700 pKa = 11.84 MLTDD8704 pKa = 2.95 MTGALAVYY8712 pKa = 8.3 NTVTQLFRR8720 pKa = 11.84 YY8721 pKa = 8.94 DD8722 pKa = 3.41 AYY8724 pKa = 11.01 GEE8726 pKa = 3.9 AVGFNKK8732 pKa = 10.17 SQAATNFLYY8741 pKa = 10.72 SGEE8744 pKa = 4.19 QFDD8747 pKa = 4.71 QRR8749 pKa = 11.84 INQQYY8754 pKa = 10.39 LRR8756 pKa = 11.84 ARR8758 pKa = 11.84 YY8759 pKa = 9.09 YY8760 pKa = 10.91 DD8761 pKa = 3.95 LSTGTFNRR8769 pKa = 11.84 LDD8771 pKa = 3.58 PFFGNLKK8778 pKa = 10.38 DD8779 pKa = 3.71 PQSLHH8784 pKa = 6.52 KK8785 pKa = 10.54 YY8786 pKa = 9.65 LYY8788 pKa = 8.94 THH8790 pKa = 7.51 ADD8792 pKa = 3.64 PVNGIDD8798 pKa = 3.28 PTGRR8802 pKa = 11.84 SFGSLVGTALVTGAWGGAIGGVAGGVLAKK8831 pKa = 10.87 FNDD8834 pKa = 3.3 QSVWKK8839 pKa = 10.26 GIGIGAASGFVGGLLLPFSAALGTAGFTAMGVTTGSAAAFGLFAGGIFAGSVSGALDD8896 pKa = 2.83 AWLRR8900 pKa = 11.84 GEE8902 pKa = 4.29 NPVEE8906 pKa = 4.0 GAMIGAFTGLIGGAVAKK8923 pKa = 10.59 AVGSSLNAIAQRR8935 pKa = 11.84 APSLTDD8941 pKa = 4.39 DD8942 pKa = 4.67 VIQGLKK8948 pKa = 10.22 GSEE8951 pKa = 3.93 VLVLGRR8957 pKa = 11.84 IQDD8960 pKa = 3.81 AVEE8963 pKa = 3.86 EE8964 pKa = 4.24 LADD8967 pKa = 4.02 EE8968 pKa = 4.17 LRR8970 pKa = 11.84 KK8971 pKa = 10.41 AGVNAYY8977 pKa = 8.01 TVADD8981 pKa = 4.16 PARR8984 pKa = 11.84 KK8985 pKa = 8.17 FAWMKK8990 pKa = 10.82 AIEE8993 pKa = 4.79 DD8994 pKa = 3.52 WAEE8997 pKa = 3.95 ANPAWFRR9004 pKa = 11.84 NVYY9007 pKa = 10.05 RR9008 pKa = 11.84 DD9009 pKa = 3.33 LANYY9013 pKa = 9.91 VSDD9016 pKa = 3.61 AGQIIRR9022 pKa = 11.84 ANKK9025 pKa = 9.57 IIQNTWDD9032 pKa = 3.6 WTSSGYY9038 pKa = 6.97 TTLEE9042 pKa = 4.1 MIEE9045 pKa = 4.0 TGIWSSVRR9053 pKa = 11.84 RR9054 pKa = 11.84 LGLSEE9059 pKa = 5.02 KK9060 pKa = 10.63 GPEE9063 pKa = 4.1 TIVRR9067 pKa = 11.84 ILRR9070 pKa = 11.84 EE9071 pKa = 3.9 RR9072 pKa = 11.84 LL9073 pKa = 3.19
Molecular weight: 973.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.923
Patrickios 0.909
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A518CS95|A0A518CS95_9PLAN DUF1080 domain-containing protein OS=Polystyrenella longa OX=2528007 GN=Pla110_38400 PE=4 SV=1
MM1 pKa = 7.38 ATRR4 pKa = 11.84 TTSKK8 pKa = 7.76 TTTKK12 pKa = 10.81 SKK14 pKa = 8.25 VTKK17 pKa = 9.96 SRR19 pKa = 11.84 RR20 pKa = 11.84 STPAAAKK27 pKa = 8.2 STKK30 pKa = 10.29 AKK32 pKa = 9.91 TSAKK36 pKa = 8.88 STAAKK41 pKa = 10.2 SGAKK45 pKa = 8.57 KK46 pKa = 6.61 TTSRR50 pKa = 11.84 TTAAKK55 pKa = 8.83 STAAKK60 pKa = 8.48 STKK63 pKa = 10.43 AKK65 pKa = 9.73 TGAAKK70 pKa = 8.98 STAAKK75 pKa = 10.24 KK76 pKa = 7.94 PTTSRR81 pKa = 11.84 SASTAKK87 pKa = 9.51 RR88 pKa = 11.84 TTAAKK93 pKa = 8.59 STKK96 pKa = 10.43 AKK98 pKa = 10.0 TSTAKK103 pKa = 8.63 PTKK106 pKa = 10.5 AKK108 pKa = 6.97 TTAAKK113 pKa = 8.71 STKK116 pKa = 10.4 AKK118 pKa = 9.93 TGTAKK123 pKa = 9.91 ATTAKK128 pKa = 9.09 KK129 pKa = 5.9 TTSRR133 pKa = 11.84 STTKK137 pKa = 10.63 SPAKK141 pKa = 9.15 RR142 pKa = 11.84 TTAAKK147 pKa = 8.64 STKK150 pKa = 8.93 TRR152 pKa = 11.84 TSAKK156 pKa = 9.25 PAAKK160 pKa = 8.83 RR161 pKa = 11.84 TTTRR165 pKa = 11.84 KK166 pKa = 5.87 TTTKK170 pKa = 9.81 RR171 pKa = 11.84 TTTRR175 pKa = 11.84 GKK177 pKa = 10.29 KK178 pKa = 9.49
Molecular weight: 18.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.479
IPC2_protein 11.447
IPC_protein 12.925
Toseland 13.115
ProMoST 13.583
Dawson 13.13
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 13.217
Grimsley 13.159
Solomon 13.598
Lehninger 13.495
Nozaki 13.115
DTASelect 13.1
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.939
IPC_peptide 13.598
IPC2_peptide 12.574
IPC2.peptide.svr19 9.201
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4607
0
4607
1712920
31
9073
371.8
41.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.803 ± 0.035
1.073 ± 0.016
5.822 ± 0.037
7.295 ± 0.039
4.029 ± 0.021
7.105 ± 0.042
2.238 ± 0.017
5.661 ± 0.025
4.503 ± 0.038
10.045 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.327 ± 0.018
3.872 ± 0.031
4.911 ± 0.028
4.41 ± 0.031
5.572 ± 0.033
6.712 ± 0.033
5.654 ± 0.042
6.648 ± 0.027
1.475 ± 0.015
2.845 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here